Determining Ruminant Materials In Feed

IngredientsLarry L. Whitlock, Sr. Ph. D.

Office of The Texas State Chemist

College Station, Texas 77841

ASFFPCO Annual Meeting

June 22-25, 2003

Fort Worth, Texas

Microscopy Equipment

• Compound Microscope

• Steroscopic Microscope

• Reagents for Spot Tests

• Accessories

Microscopic Procedures

• Use a 20 gram Sample for Viewing with the Stereomicroscope

• Take a Separate 10 gram Sample for Floatation

• Observe Mineral Portion

• Spot Tests for Confirmation

Spot Testing Reagants

Examples of Spot Testing• 10% HC1 for Carbonates, Limestone, or

Oyster Shell• Nesslers Reagent for Ammonium Containing

Compounds• Urea Spot Testing• Silver Nitrate for Chlorides• Quimociac for Phosphates

NOTE:Work with all floatation procedures in a hood to prevent breathing the vapors from the solvent

Application of Microscopy

• Levels of Meat and Bone in Feed Sample

• Identify Milk and Dried Blood

• Microscopy is a Rapid Procedure

• OTSC Current Surveys

Meat and Bone in Feed

Meat and Bone Meal in Feed

Meat and Bone Meal in Feed

Meat and Bone Meal in Feed

Bone Fragment

Animal Bone in Feed

Blood Meal in Feed

Meat and Bone Meal

Poultry Meal

Feather Meal

Hydrolyzed Feathers

Agri-Screen by Neogen Corporation

• Neogen has Two Test Strips

• Presence of Ruminant Meat and Bone in Animal Protein Products

• Presence of Ruminant Meat and Bone in Feeds

Agri-Screen Procedures for Ruminant M&B Meal

• Weigh 10 grams Sample in Mason Type Jar• Add 100 ml Extraction Solution• Place in Boiling Water Bath for 10 Minutes• Remove Mason Jar from Water Bath• Prepare Sample Tubes and Transfer 0.5 ml of

Extract to a Tube (Use different pipette for each sample)

• Place Sample Strip into each Sample Tube• Develop for 10 Minutes and Read Results

Reading Strip Results

• Negative-Control Line Appears Only

• Postive-Control Line and Sample Line Appear

Discussion