DEVELOPMENT AND COMMERCIALIZATION FOR BIO-PESTICIDE BY USING PSEUDOMONAS

FLUORESCENCE

INTRODUCTION P. fluorescence is gram negative bacillus

These bacteria are easy to culture, as they grow on minimal media and at 25-30 degrees Celsius

They destroy certain toxins and pollutants including styrene, polycyclic aromatic hydrocarbons, and TNT

They also protect plants from infection by pathogens by producing secondary metabolites like antibiotics and hydrogen cyanide which kill other bacteria and fungi

ABSTRACT In physical characteristic test in various media P. fluorescence shows positive growth

In biochemical characteristic on various media P. fluorescence shows positive result

In anti-pathogenic activity test of P. fluorescence also shows positive result against F. oxysporum. This test confirm that P. fluorescence can be use as bio-fertilizer

MATERIAL AND METHOD All the pure culture of P. fluorescence are taken from DESIGN

BIOSIS

All chemical & media were prepared to evaluate the biochemical assay, physical appearance & anti-pathogenic activity test of P. fluorescence

Also microscopic observation of P. fluorescence was done

Physical activity test

various media was used to determine the physical appearance of P. fluorescenceThose are King’s B agar media King’s Broth media Starch agar and starch broth media LB Broth media pikovaskaya's agar and broth media

Biochemical activity test

various media was use to determine the Biochemical activity test for P. fluorescenceThose are Citrate agar slant media Starch agar media Czapek agar medium

Anti-pathogenic activity test

various media was use to determine the anti-pathogenic activity test for P. fluorescenceThose are LB agar media Potato dextrose agar medium

Physical characteristic photos

King’s B agar medium

King’s B Broth media LB Broth media

Starch Broth media Starch agar media

Pikovaskaya's agar media Pikovaskaya's agar media

Biochemical activity test photos

Citrate agar medium

Starch agar medium

Czapek agar medium

Anti-pathogenic activity testIn PDA media F.oxysporum

P. fluorescence

Mat based inhibition of F.oxysporium(Plant Pathogen)

In PDA media

F. oxysporum P. fluorescence F.oxysporum

Inoculum based inhibition of F.oxysporium (Plant -Pathogen)

In LB agar mediaF. oxysporum

P. fluorescence

F. oxysporium is inoculated in middle and P. fluorescence Inoculated in around that F. oxysporium(Plant pathogen)

In LB agar media

F. oxysporum

P. fluorescence

Mat based inhibition of F.oxysporum (Plant Pathogen)

Mass Culture, Bio-formulation and commercialization

Blander

Mass culture ofPseudomonas fluorescence

Market product

RESULTPhysical activity test

From the above photos we can observed that in starch agar media the growth of Pseudomonas fluorescence is greater than Kings B media, pikovaskaya's media.

In fact in pikovaskaya's agar slant media was to less.

Biochemical activity test The biochemical assay confirmed that Pseudomonas

fluorescence has citrate utilizing, starch hydrolysis and cellulose activity.

This experiment shows the proof and practical identification

and confirmation method for P. fluorescence.

Anti-pathogenic activity test The anti-pathogenic activity of P. fluorescence against F.

oxysporum shows that the mat based formulation of bio-fertilizer is effective against F. oxysporum rather than liquid based preparation.

Also P. fluorescence can be added in combination with other soil friendly microorganisms to inhibit of the plant pathogens including F. oxysporum in it’s soil based application

Mass Culture, Bio-formulation and commercialization

The bio-formulation of P. fluorescence is an easy way if can be done by P. fluorescence Mat mixing with Talc powder and CMC.

The commercialization of this bio-formulation can be widely used as the plant pathogen eradication or control by using natural resources are very few in Agriculture sector.

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