Difco - BD

Difco & BBL ManualManual of Microbiological Culture Media

Second Edition

Editors

Mary Jo Zimbro, B.S., MT (ASCP) David A. Power, Ph.D.

Sharon M. Miller, B.S., MT (ASCP)George E. Wilson, MBA, B.S., MT (ASCP)

Julie A. Johnson, B.A.

BD Diagnostics – Diagnostic Systems7 Loveton Circle

Sparks, MD 21152

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Contents

Preface ...............................................................................................................................................................v

About This Manual ...........................................................................................................................................vii

History of BD Diagnostics .................................................................................................................................ix

Section I: Monographs .......................................................................................................................................1History of Microbiology and Culture Media ...................................................................................................3Microorganism Growth Requirements .............................................................................................................4Functional Types of Culture Media ..................................................................................................................5Culture Media Ingredients – Agars ...................................................................................................................6Culture Media Ingredients – Peptones and Hydrolysates ..................................................................................7Media Sterilization .........................................................................................................................................13Quality Control Organisms............................................................................................................................15Typical Analyses .............................................................................................................................................17

Section II: General Technical Information ........................................................................................................19Dehydrated Culture Media ............................................................................................................................21Prepared Plated Media ...................................................................................................................................25Prepared Tubed, Bottled and Mycoflask™ Media ............................................................................................27

Section III: Culture Media and Ingredients .......................................................................................................31

Section IV: Reference Guide ...........................................................................................................................635Culture Media for Specific Groups of Microorganisms ................................................................................637Application Tables .......................................................................................................................................644

Agar Selection Guide ................................................................................................................................644Antimicrobial Effectiveness Testing ...........................................................................................................645Antimicrobial Residue Testing ..................................................................................................................646Bionutrient Selection Guide ......................................................................................................................647Cosmetic Testing .......................................................................................................................................648Environmental Sampling ...........................................................................................................................650Food, Dairy and Beverage Testing .............................................................................................................651Food Testing for E. coli O157:H7 using BBL™ CHROMagar™ O157 ......................................................655Food Testing for Listeria using BBL™ CHROMagar™ Listeria ..................................................................656Food Testing for Salmonella using BBL™ CHROMagar™ Salmonella ........................................................657Food Testing for Staphylococcus aureus using BBL™ CHROMagar™ Staph aureus ...................................658Molecular Genetics Selection Guide ..........................................................................................................659Pharmaceutical Testing per USP ................................................................................................................660USP Chapter <61>: Microbial Enumeration Tests .....................................................................................661USP Chapter <62>: Tests for Specified Organisms ....................................................................................662Veterinary Testing .....................................................................................................................................663Water/Wastewater Testing .........................................................................................................................666Water Testing for Enterococcus using BBL™ mEI Agar .............................................................................669Water Testing for E. coli using BBL™ Modified mTEC Agar ......................................................................670Water Testing for Total Coliforms and E. coli using BBL™ MI Agar ..........................................................671

Product Tables .............................................................................................................................................672Culture Media – Antimicrobial Susceptibility Testing................................................................................672Culture Media – General-Purpose .............................................................................................................672Culture Media – Supplements/Selective Agents .........................................................................................673Peptones and Hydrolysates (product listing) .............................................................................................674Peptones by Category ...............................................................................................................................675Typical Analyses – Peptones and Hydrolysates ..........................................................................................676

Product Index ..................................................................................................................................................679

Table of Contents


First Edition 2003Second Edition 2009

Copyright 2009 by Becton, Dickinson and Company

Sparks, Maryland 21152 USA

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CDC Anaerobe 5% Sheep Blood Agar CDC Anaerobe 5% Sheep Blood Agar with Kanamycin and Vancomycin (KV) • CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV)Intended Use CDC Anaerobe 5% Sheep Blood Agar is used for the isolation and cultivation of fastidious and slow growing, obligately anaerobic bacteria from a variety of clinical and nonclini-cal materials. It also supports good growth of most aerobic, facultatively anaerobic and microaerophilic bacteria if incubated appropriately.

CDC Anaerobe 5% Sheep Blood Agar with Kanamycin and Vancomycin (KV), CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA) and CDC Anaerobe Laked Sheep Blood Agar with Kanamycin and Vancomycin (KV) are used for the selective isolation of fastidious and slow-growing, obligately anaerobic bacteria from a variety of clinical and nonclinical materials.

Summary and Explanation The isolation of obligately anaerobic bacteria from clinical and nonclinical materials requires the use of selective, nonselective and enrichment media.1 The choice of media to be employed is based upon the type of material and the results of direct microscopic observation. Nonselective media are used to isolate organisms present in low numbers and to provide an indication of the numbers and types of organisms present in the specimen or sample. Selective media are employed to facilitate recovery of the desired organisms present in mixed populations.

CDC Anaerobe 5% Sheep Blood Agar was formulated by Dowell et. al. of the Center for Disease Control (currently named the Centers for Disease Control and Prevention CDC) as an enriched, nonselective medium for the isola-tion and cultivation of a wide variety of obligately anaerobic microorganisms, particularly those found in clinical materials.2-5 The medium employs BBL™ Trypticase™ Soy Agar supple-mented with additional agar, yeast extract, vitamin K1, hemin, cystine and 5% sheep blood. Improved growth of Prevotella melaninogenica, Fusobacterium necrophorum, Clostridium haemolyticum, as well as certain strains of Actinomyces israelii and Bacteroides thetaiotaomicron, has been demonstrated on this medium.3 Furthermore, less smooth to rough colonial variation has been reported on this medium than on Schaedler Blood Agar.6

CDC Anaerobe 5% Sheep Blood Agar with Kanamycin and Vancomycin was formulated as an enriched, selective medium for the isolation of obligately anaerobic, gram-negative bacilli from clinical materials.2 The medium employs Trypticase Soy Agar supplemented with additional agar, yeast extract, vitamin K1, hemin, cystine, 5% sheep blood, kanamycin and vancomycin. The combination of kanamycin and vancomycin for use in selective isolation of gram-negative anaerobes was first described by Finegold et. al.7 Vancomycin, however, may inhibit Porphyromonas asaccharolytica.8

CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol was formulated as an enriched selective medium for the isolation and cultivation of obligately anaerobic bacteria from clinical materials containing rapidly growing, facultatively anaerobic bacteria such as Proteus and other members of the family Enterobacteriaceae.2 The medium employs Trypticase Soy Agar supplemented with additional agar, yeast extract, vitamin K1, hemin, cystine, 5% sheep blood and phenylethyl alcohol.

CDC Anaerobe Laked Blood Agar with Kanamycin and Vancomycin was formulated as an enriched, selective medium for the isolation and cultivation of Prevotella melaninogenica, Fusobacterium necrophorum, Fusobacterium nucleatum and other fastidious, obligately anaerobic, gram-negative bacilli, from clinical materials containing mixed populations.1,3 The medium employs Trypticase Soy Agar supplemented with additional agar, yeast extract, vitamin K1, hemin, cystine, 5% sheep blood, kanamycin and vancomycin. The combination of kanamycin and vancomycin for use in selective isolation of gram-negative anaerobes was first described by Finegold et. al.7 Vancomycin, however, may inhibit Porphyromonas asaccharolytica.8 This medium is similar to CDC Anaerobe 5% Sheep Blood Agar and Kanamycin and Vancomycin except that the blood has been laked, by subjecting it to three freeze-thaw cycles, for improved pigmentation of the P. melaninogenica-P. asaccharolytica group.9

Principles of the Procedure CDC Anaerobe 5% Sheep Blood Agar is a highly nutritious medium due to its content of peptones, yeast extract, hemin, vitamin K1 and sheep blood. The peptones provide nitrogenous growth factors, carbon, sulfur and trace ingredients. Yeast extract is an important source of B vitamins. Sodium chloride

CDC Anaerobe 5% SB Agar

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CDC Anaerobe 5% Sheep Blood AgarClostridium perfringensATCC™ 13124

Bacteroides fragilisATCC™ 25285

CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA)

Peptostreptococcus anaerobiusATCC™ 27337

CDC Anaerobe Agar with Laked Sheep Blood and KVPorphyromonas leviiATCC™ 29147

Bacteroides fragilisATCC™ 25285

maintains osmotic equilibrium. Sheep blood constituents, hemin, cystine and vitamin K1 provide growth factors required by certain obligate anaerobes.3-6,10,11

The addition of the antimicrobial agents, kanamycin and vancomycin, renders the medium selective for gram-negative microorganisms. The kanamycin inhibits protein synthesis in susceptible organisms, whereas the vancomycin inhibits gram-positive bacteria by interfering with cell wall synthesis.12

Selectivity is achieved through the addition of phenylethyl alcohol which reduces the growth of facultatively anaerobic, gram-negative bacteria without inhibiting growth of obligately anaerobic bacteria.13

The addition of laked sheep blood to CDC Anaerobe Agar improves pigmentation of the Prevotella, Porphyromonas, and pigmented Bacteroides species.12

Divided Petri dishes containing CDC Anaerobe 5% Sheep Blood Agar in each half enable two specimens to be streaked on one plate. Combinations of selective media are also provided in divided dishes.

Procedure Streak the specimen as soon as possible after it is received in the laboratory. Minimize exposure to air. With liquid specimens, media should be inoculated with one drop of the specimen. Tissue specimens should be minced and then ground in sterile broth, such as BBL Enriched Thioglycollate Medium, before inoculation. Inoculation is then performed as for liquid speci-mens. Swab specimens may be rolled onto the first quadrant of plated media and then used to inoculate liquid media. Alternatively, the swab may be “scrubbed” in a small volume

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of reduced broth and the broth used to inoculate media as performed with liquid specimens.

These media should be reduced immediately prior to inoculation by placing under anaerobic conditions for 18-24 hours.3 An efficient and easy way to obtain suitable anaerobic conditions is through the use of BD GasPak™ EZ anaerobic systems or an alternative anaerobic system.14

Plated media should be inoculated using the streak plate method in order to obtain pure cultures from specimens containing mixed flora.

An enrichment broth, such as BBL Enriched Thioglycollate Medium, should be inoculated at the same time as the primary isolation plates.

Incubate immediately under anaerobic conditions or place in a holding jar flushed with oxygen free gas(es) until sufficient plates are accumulated (but no longer than 3 hours).14 Incubation should be at 35 ± 2°C for at least 48 hours and up to 7 days.

Expected Results Examine colonies using a dissecting microscope and a long-wave UV lamp (colonies of the pigmenting Porphyromonas- Prevotella species should fluoresce orange to brick-red under UV light). Fluorescence is visible before pigmentation.

In order to determine the relationship to oxygen of each colony type present on anaerobic solid media, inoculate the following media:15

1. One anaerobe blood agar plate to be incubated anaerobi-cally.

2. One aerobic blood agar (or chocolate agar) plate to be incubated in an aerobic atmosphere enriched with carbon dioxide. The chocolate agar is particularly needed to distinguish nutritionally-fastidious Haemophilus species and other bacteria which will grow on anaerobe blood agar incubated anaerobically and on chocolate agar under increased carbon dioxide tension but which fail to grow on blood agar in the presence of carbon dioxide or in the air.

3. One aerobic blood agar plate to be incubated aerobically without added carbon dioxide.

4. Tubes of Enriched Thioglycollate Medium and/or Cooked Meat Medium and a tube of Peptone Yeast Extract Glucose Broth.

Incubate all cultures at 35 ± 2°C for a minimum of 24 hours and up to 7 days.

Record the relationship to oxygen as either obligate anaerobe or nonanaerobe (aerotolerant anaerobe, microaerophilic, or facultative anaerobe).15

Colonies of the type(s) which proved to be obligate anaerobes can be further studied using the corresponding broth cultures.

Organisms failing to grow on the aerobic subculture plates may be presumed to be obligately anaerobic in terms of their oxygen requirements.

References1. Dowell. 1975. In Balows (ed.), Clinical microbiology. How to start and when to stop Charles

C. Thomas, Springfield, Ill.2. Dowell, Lombard, Thompson and Armfield. 1977. Media for isolation, characterization, and

identification of obligately anaerobic bacteria. CDC laboratory manual. Center for Disease Control, Atlanta, Ga.

3. Dowell and Hawkins. 1987. Laboratory methods in anaerobic bacteriology. CDC laboratory manual. HHS Publication (CDC) 87-8272. Center for Disease Control, Atlanta, Ga.

4. Forbes and Granato. 1995. In Murray, Baron, Pfaller, Tenover and Yolken (ed.), Manual of clinical microbiology, 6th ed. American Society for Microbiology, Washington, D.C.

5. Rodloff, Applebaum and Zabransky. 1991. Cumitech 5A, Practical anaerobic bacteriology. Coord. ed., Rodloff. American Society for Microbiology, Washington, D.C.

6. Star, Killgore and Dowell. 1971 Appl. Microbiol. 22:655.7. Finegold, Miller, and Posnick. 1965. Ernahrungsforschung. 10:517.8. van Winkelhoff and de Graaf. 1983. J. Clin. Microbiol. 18:1282.9. Finegold and Citron. 1980. In Lennette, Balows, Hausler and Truant (ed.), Manual of clinical micro-

biology, 3rd ed. American Society for Microbiology, Washington, D.C.10. Gibbons and MacDonald. 1960. J. Bacteriol. 80:164.11. Wilkins, Chalgren, Jimenez-Ulate, Drake and Johnson. 1976. J. Clin. Microbiol. 3:359.12. Estevez. 1984. Lab. Med. 15:258.13. Dowell, Hill and Altemeir. 1964. J. Bacteriol. 88:1811.14. Martin. 1971. Appl. Microbiol. 22:1168.15. Allen, Siders and Marler. 1985. In Lennette, Balows, Hausler, and Shadomy (ed.), Manual of clinical

microbiology, 4th ed. American Society for Microbiology, Washington, D.C.

Availability BBL™ CDC Anaerobe 5% Sheep Blood AgarBS12 CMPH2 MCM9

United States and Canada Cat. No. 221733 Prepared Plates – Pkg. of 20* 221734 Prepared Plates – Ctn. of 100*

Europe Cat. No. 256506 Prepared Plates – Pkg. of 20*

Japan Cat. No. 251733 Prepared Plates – Pkg. of 20* 251584 Prepared Plates (150 × 15 mm-style) – Pkg. of 24*

BBL™ CDC Anaerobe 5% Sheep Blood Agar with Kanamycin and Vancomycin (KV)CMPH2 MCM9

Cat. No. 221735 Prepared Plates – Pkg. of 20* 221736 Prepared Plates – Ctn. of 100*

BBL™ CDC Anaerobe 5% Sheep Blood Agar with Phenylethyl Alcohol (PEA)BS12 CMPH2 MCM9

Cat. No. 221739 Prepared Plates – Pkg. of 20*

BBL™ CDC Anaerobe Agar with Laked Sheep Blood and KVCMPH2 MCM9

Cat. No. 221846 Prepared Plates – Pkg. of 20*

BBL™ CDC Anaerobe 5% Sheep Blood Agar// CDC Anaerobe 5% Sheep Blood Agar with PEACat. No. 297646 Prepared Bi-Plate Dishes – Pkg. of 20*

BBL™ CDC Anaerobe 5% Sheep Blood Agar with KV//CDC Anaerobe 5% Sheep Blood Agar with PEACat. No. 297004 Prepared Bi-Plate Dishes – Pkg. of 20*

BBL™ CDC Anaerobe 5% Sheep Blood Agar with PEA//Anaerobe Laked Sheep Blood Agar with KVCat. No. 299611 Prepared I Plate™ Dishes – Pkg. of 20**Store at 2-8°C.

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Product Index

AA-1 Medium .................................................................................33A7 Agar, Modified ........................................................................34AC Broth ......................................................................................35Acetamide Agar ............................................................................38Acetate Differential Agar...............................................................39Acidicase™ Peptone .............................................................40, 126Actinomyces Broth ........................................................................40Actinomycete Isolation Agar .........................................................41Agar, 1.5% ...................................................................................45Agar, Bacto™ ................................................................................42Agar, Grade A ...............................................................................42Agar, Granulated ..........................................................................42Agar, Noble ..................................................................................42Agar, Select ..................................................................................42Agar, Technical ..............................................................................42Agarose ........................................................................................42Agars ............................................................................................42AK Agar #2 (Sporulating Agar) ......................................................36Alkaline Peptone Water.................................................................45Amies Transport Media .........................................................46, 559Amino Acid Assay Media (Lysine and Cystine) ...............................46Anaerobe Broth MIC .............................................................48, 615Anaerobe CNA Agar with 5% Sheep Blood ..................................48Anaerobe Laked Sheep Blood KV Agar ..........................................49Anaerobe Neomycin 5% Sheep Blood Agar ..................................50Anaerobic Agar .............................................................................50Antibiotic Assay Broth ...................................................................52Antibiotic Assay Media ..................................................................52Antibiotic Medium 1 .....................................................................52Antibiotic Medium 2 .....................................................................52Antibiotic Medium 3 .....................................................................52Antibiotic Medium 4 .....................................................................52Antibiotic Medium 5 .....................................................................52Antibiotic Medium 8 .....................................................................52Antibiotic Medium 9 .....................................................................52Antibiotic Medium 10 ...................................................................52Antibiotic Medium 11 ...................................................................52Antibiotic Medium 12 ...................................................................52Antibiotic Medium 13 ...................................................................52Antibiotic Medium 19 ...................................................................52Antimicrobic Vial A .....................................................................163Antimicrobic Vial K .....................................................................480Antimicrobic Vial P ..............................................................327, 480APT Agar ......................................................................................37APT Broth .....................................................................................37Arylsulfatase Broth (0.001 M and 0.003 M) ...................................57Aspergillus Differential Agar ..........................................................58Azide Blood Agar Base ..................................................................59Azide Dextrose Broth ....................................................................60

BB12 Assay Medium .........................................................................61B12 Culture Agar ...........................................................................63B12 Inoculum Broth ........................................................................63Bacteroides Bile Esculin Agar (BBE) ................................................70Baird-Parker Agar Base ..................................................................71Baird-Parker Agar ..........................................................................71BCYE Agar ....................................................................................64BCYE Agar Base ............................................................................64BCYE Differential Agar ..................................................................64BCYE Selective Agars (CCVC, PAC, PAV) .......................................64Beef Extract ..................................................................................73Beef Extract, Desiccated, Bacto™...................................................73Beef Extract Powder ......................................................................73Beef Heart for Infusion ..................................................................74BG Sulfa Agar ...............................................................................67BiGGY Agar ..................................................................................69Bile Esculin Agar ...........................................................................75Biosate™ Peptone ........................................................................76Biotin Assay Medium ....................................................................77

Bird Seed Agar ..............................................................................79Bismuth Sulfite Agar .....................................................................79Blood Agar Base (Infusion Agar) ....................................................82Bordet Gengou Agar Base .............................................................84Bordet Gengou Blood Agar ...........................................................84Bovine Albumin 5% ......................................................................85Brain Heart CC Agar .....................................................................86Brain Heart Infusion ......................................................................88Brain Heart Infusion with Supplements ..........................................88Brain Heart Infusion without Dextrose ...........................................88Brain Heart Infusion Broth, Modified .............................................88Brain Heart Infusion Agar ..............................................................90Brain Heart Infusion Agar, Modified ..............................................90Brain Heart Infusion Agars, Selective .............................................86Brain Heart Infusion Sheep Blood Agar ..........................................90Brain Heart Infusion, Porcine, Bacto™ ............................................93Brain Heart Infusion with PAB and Agar ........................................94Brain Heart Infusion with PABA .....................................................94Brewer Anaerobic Agar .................................................................95Brilliant Green Agar ......................................................................96Brilliant Green Agar Modified ........................................................97Brilliant Green Bile Agar ................................................................99Brilliant Green Bile Broth 2%.......................................................100m Brilliant Green Broth................................................................101Brucella Agar ..............................................................................103Brucella Agar with 5% Horse Blood ............................................103Brucella Agar with 5% Sheep Blood, Hemin and Vitamin K1 ........105Brucella Broth .............................................................................103Brucella Broth with 20% Glycerol................................................106Brucella Laked Sheep Blood Agar

with Kanamycin and Vancomycin ............................................105Buffered Listeria Enrichment Broth Base ..............................106, 303Buffered Peptone Water ..............................................................106Buffered Peptone Casein Water ..................................................106Buffered Sodium Chloride-Peptone Solution................................108Bushnell-Haas Broth ....................................................................109

CCampy CSM Agar .......................................................................120Campy CVA Agar ........................................................................120Campy-Cefex Agar .....................................................................119Campylobacter Agar Base ...........................................................120Campylobacter Agar with 5 Antimicrobics

and 10% Sheep Blood ............................................................120Camplyobacter Antimicrobic Supplement Blaser ..........................120Camplyobacter Antimicrobic Supplement Skirrow .......................120Campylobacter Thioglycollate Medium

with 5 Antimicrobics ...............................................................123Candida BCG Agar Base .............................................................124Candida Bromcresol Green Agar .................................................124Cary and Blair Transport Medium ........................................126, 559Casamino Acids ..........................................................................126Casamino Acids, Bacto™ .............................................................126Casamino Acids, Technical, Bacto™ .............................................126Casamino Acids, Vitamin Assay ...................................................126Casein Agar ........................................................................128, 396Casein Digest ..............................................................................128Casitone, Bacto™ ........................................................................128Casman Agar Base ......................................................................131CDC Anaerobe 5% Sheep Blood Agar ........................................110CDC Anaerobe 5% Sheep Blood Agar

with Kanamycin and Vancomycin ............................................110CDC Anaerobe 5% Sheep Blood Agar

with Phenylethyl Alcohol .........................................................110CDC Anaerobe Laked Sheep Blood Agar

with Kanamycin and Vancomycin ............................................110Cetrimide Agar Base ...................................................................132Chapman Stone Medium ............................................................134Charcoal Agar .............................................................................135Chocolate II Agar ........................................................................136Chocolate II Agar with Bacitracin ................................................136Chocolate II Agar with Pyridoxal ..................................................136

Product Index

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Choline Assay Medium ...............................................................138Chopped Meat Carbohydrate Broth, PR II ....................................139Chopped Meat Glucose Broth, PR II ............................................139CHROMagar™ Candida ..............................................................140CHROMagar™ Listeria ................................................................141CHROMagar™ MRSA .................................................................143CHROMagar™ MRSA II ...............................................................145CHROMagar™ O157 ..................................................................145CHROMagar™ Orientation .........................................................147CHROMagar™ Salmonella ..........................................................149CHROMagar™ Staph aureus .......................................................151CIN Agar .....................................................................................113CLED Agar ..................................................................................114Clostridium Difficile Selective Agar ..............................................153Coagulase Mannitol Agar ...........................................................154Columbia Agar Base ...................................................................155Columbia Agar with 5% Sheep Blood .........................................155Columbia Agar with Fildes Enrichment and Bacitracin .................155Columbia Anaerobe 5% Sheep Blood Agar .................................159Columbia Blood Agar Base ..........................................................155Columbia Blood Agar Base EH ....................................................155Columbia Broth ..........................................................................160Columbia CNA Agar ...................................................................161Columbia CNA Agar, Modified ....................................................161Columbia PNA Agar ....................................................................161Cooke Rose Bengal Agar .............................................................163Cooked Meat Medium ................................................................164Cooked Meat Medium with Glucose, Hemin and Vitamin K1 .......164Corn Meal Agar ..........................................................................166Corn Meal Agar with 1% Dextrose .............................................166Corn Meal Agar with Polysorbate 80...........................................166CTA Agar ....................................................................................116CTA Medium™ ...........................................................................117CTA Medium™ with Carbohydrates ............................................117Cystine Assay Medium ..........................................................46, 167Cystine Heart Agar ......................................................................167Cystine Tryptic Agar ............................................................117, 169Czapek Solution Agar .................................................................169Czapek-Dox Broth .......................................................................169

DD/E Neutralizing Agar ..................................................................172D/E Neutralizing Broth .................................................................172DCLS Agar ..................................................................................170Decarboxylase Base Moeller ........................................................179Decarboxylase Differential Media ................................................179Decarboxylase Medium Base .......................................................179Demi-Fraser Broth Base ...............................................................183Dermatophyte Test Medium Base ................................................184Dermatophyte Test Medium, Modified with Chloramphenicol .....184Desoxycholate Agar ....................................................................186Desoxycholate Citrate Agar .........................................................187Desoxycholate Lactose Agar ........................................................189Dextrose Agar .............................................................................190Dextrose Broth ............................................................................190Dextrose Starch Agar ..................................................................191Dextrose Tryptone Agar ...............................................................192Differential Reinforced Clostridial Agar ........................................193DNase Test Agar ..........................................................................175DNase Test Agar with Methyl Green ............................................175DNase Test Agar with Toluidine Blue ............................................175DRBC Agar .................................................................................178Dubos Broth Base .......................................................................195Dubos Broth, Enriched ................................................................195Dubos Medium Albumin .............................................................195Dubos Oleic Agar Base ................................................................195Dubos Oleic Albumin Complex ....................................................195

Em E Agar ....................................................................................197EC Medium .................................................................................200EC Medium, Modified .................................................................202EC Medium with MUG ................................................................201EE Broth Mossel Enrichment ........................................................204Egg Yolk Agar, Modified .............................................................209Egg Yolk Enrichment 50% ..................................................351, 480

mEI Agar .....................................................................................206Elliker Broth ................................................................................210EMB Agar, Levine, without Lactose..............................................222Endo Agar ..................................................................................211m Endo Agar LES ........................................................................212m Endo Broth MF™ ......................................................................213Enriched Thioglycollate Media .............................................215, 547Enriched Thioglycollate Medium ..................................................547Enriched Thioglycollate Medium with Calcium Carbonate ...........547Enteric Fermentation Base ...........................................................215Enterococcosel™ Agar ...............................................................216Enterococcosel™ Agar with Vancomycin, 8 µg/mL .....................218Enterococcosel™ Broth ..............................................................216m Enterococcus Agar ..................................................................219 Enterococcus Screen Agar QUAD Plate ........................................220Eosin Methylene Blue Agar, Levine ..............................................222Eosin Methylene Blue Agar, Modified, Holt-Harris and Teague .....224Esculin Agar ................................................................................225Esculin Iron Agar .................................................................197, 225Eugon Agar ................................................................................226Eugon Broth, Bacto™ ..................................................................227Eugonbroth™ Medium ...............................................................227EVA Broth ...................................................................................208EY Tellurite Enrichment .................................................................71

Fm FC Agar ..................................................................................228m FC Basal Medium ....................................................................230m FC Broth Base .........................................................................228Ferric Chloride Reagent ...............................................................433Fildes Enrichment ........................................................................232Fletcher Medium Base .................................................................233Fletcher’s Medium .......................................................................233Fletcher’s Medium with 5-FU .......................................................233FLN Agar .....................................................................................231Flo Agar ..............................................................................234, 454Fluid A ........................................................................................234Fluid D ........................................................................................234Fluid Sabouraud Medium ....................................................235, 490Fluid Thioglycollate Media ...................................................235, 490Fluid Thioglycollate Medium ........................................................547Fluid Thioglycollate Medium, Enriched ........................................547Fluid Thioglycollate Medium without Dextrose ............................547Fluid Thioglycollate Medium with Beef Extract .............................547Folic Acid Assay Medium .............................................................237Folic Acid Casei Medium .............................................................239Folic AOAC Medium ...................................................................235Fraser Broth Base ........................................................................241Fraser Broth Supplement .....................................................183, 241FTA Hemagglutination Buffer ......................................................232

GGC II Agar with IsoVitaleX™ Enrichment ....................................247GC Medium Base ........................................................................242GC-Lect™ Agar ...........................................................................248Gelatin ........................................................................................251Gelysate™ Peptone ....................................................................252Giolitti-Cantoni Broth Base ..........................................................253GN Broth ....................................................................................250GN Broth, Hajna .........................................................................250M-Green Yeast and Mold Broth ...................................................255Group A Selective Strep Agar with 5% Sheep Blood (ssA™) ........256

HHaemophilus Isolation Agar with Bacitracin .................................260Haemophilus Test Medium Agar (HTM Agar) ...............................261HBT Bilayer Medium ....................................................................257HC Agar Base .............................................................................258Heart Infusion Agar .....................................................................262Heart Infusion Broth, Bacto™ ......................................................264Hektoen Enteric Agar ..................................................................265Hemo (Haemophilus) Identification (ID) QUAD Plate ....................266Hemoglobin ................................................................................242Herrold’s Egg Yolk Agar with Mycobactin J and ANV ...................268Herrold’s Egg Yolk Agar without Mycobactin J with ANV .............268m HPC Agar ................................................................................259


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IIndole Nitrite Medium (Trypticase™ Nitrate Broth) ......................270Infusion Agar (Blood Agar Base) ....................................................82Inhibitory Mold Agar ...................................................................271Inhibitory Mold Agar with Gentamicin ........................................271Inositol Assay Medium ................................................................273IsoVitaleX™ Enrichment .............................................................242ISP Medium 1 .............................................................................269ISP Medium 2 .............................................................................269ISP Medium 4 .............................................................................269

JJordan’s Tartrate Agar .................................................................274

KKF Streptococcus Agar ................................................................275KF Streptococcus Broth ...............................................................276Kligler Iron Agar ..........................................................................278Koser Citrate Medium .................................................................280

LLactobacilli Agar AOAC ...............................................................287Lactobacilli Broth AOAC ..............................................................287Lactobacilli MRS Agar .................................................................289Lactobacilli MRS Broth ................................................................289Lactose Broth ..............................................................................290Lactose Peptone Broth ................................................................292Lauryl Sulfate Broth .....................................................................293Lauryl Sulfate Broth with MUG ....................................................294Lauryl Tryptose Broth ...................................................................293Lauryl Tryptose Broth with MUG ..................................................294LB Agar, Lennox ..........................................................................281LB Agar, Miller ............................................................................282LB Broth Base (Animal Free).........................................................283LB Broth, Lennox .........................................................................281LB Broth, Miller ...........................................................................282LBS Agar .....................................................................................284LBS Broth ....................................................................................284Lecithin Lactose Agar ..................................................................296Legionella Agar Base .....................................................................64Legionella Agar Enrichment ..........................................................64Legionella Selective Agar .............................................................297Legionella Selective Agar DGVP ...................................................297Leptospira Enrichment EMJH .......................................................298Leptospira Medium Base EMJH ...................................................298Letheen Agar ..............................................................................299Letheen Agar, Modified ..............................................................301Letheen Broth .............................................................................299Letheen Broth, Modified .............................................................301Levine EMB Agar .................................................................222, 302Lim Broth ....................................................................................302Lipase Reagent ............................................................................513Listeria Enrichment Broth ............................................................303Listeria Enrichment Broth, Modified ............................................303Listeria Enrichment Broth Base, Buffered .....................................303Listeria Selective Supplement ......................................................285Litmus Milk .................................................................................305Liver Infusion Agar ......................................................................307Liver Infusion Broth .....................................................................307Liver Veal Agar ............................................................................308Lowenstein Medium Base ...........................................................309Lowenstein-Jensen Medium ........................................................309Lowenstein-Jensen Medium, Gruft ..............................................309Lowenstein-Jensen Medium with 5% Sodium Chloride ...............309Lowenstein-Jensen Medium with Iron .........................................309Lowenstein-Jensen Medium with Pyruvic Acid .............................309LPM Agar Base ............................................................................285Luria Agar Base, Miller ................................................................312Luria Broth Base, Miller ...............................................................312Lysine Assay Medium ............................................................46, 313Lysine Decarboxylase Broth .................................................179, 313Lysine Iron Agar ..........................................................................313Lysozyme Broth ...........................................................................315Lysozyme Control ........................................................................315

MM Broth, Bacto™ .........................................................................318M17 Agar ...................................................................................316M17 Broth ..................................................................................316M9 Minimal Salts, 5× .................................................................316MacConkey Agar ........................................................................328MacConkey Agar Base ................................................................328MacConkey Agar without Crystal Violet ......................................328MacConkey Agar without Crystal Violet or Salt ...........................328MacConkey Agar without Salt ....................................................328MacConkey Agars with Sorbitol ..................................................335MacConkey Broth .......................................................................337MacConkey II Agar .....................................................................333MacConkey II Agar with MUG ....................................................333MacConkey II Agar with Sorbitol .................................................335MacConkey Sorbitol Agar ...........................................................335Malonate Broth ...........................................................................339Malonate Broth, Ewing Modified ................................................340Malt Agar ...................................................................................341Malt Extract, Bacto™ ...................................................................343 Malt Extract Agar ........................................................................343Malt Extract Broth .......................................................................343Mannitol Salt Agar ......................................................................345Marine Agar 2216 ......................................................................347Marine Broth 2216 .....................................................................347Martin-Lewis Agar ......................................................................348Martin-Lewis Agar (Gono-Pak) ....................................................348Martin-Lewis Agar (JEMBEC™) .....................................................348Martin-Lewis Agar, Modified .......................................................348Maximum Recovery Diluent .........................................................350McClung Toabe Agar Base ..........................................................351McFarland Turbidity Standard No. 0.5 .........................................352MI Agar ......................................................................................319Micro Assay Culture Agar............................................................353Micro Inoculum Broth .................................................................353Microbial Content Test Agar ................................................354, 571Middlebrook 7H10 Agar .............................................................356Middlebrook 7H9 Broth ..............................................................355Middlebrook 7H9 Broth with Glycerol .........................................355Middlebrook 7H9 Broth with Polysorbate 80 ...............................355Middlebrook ADC Enrichment ....................................................355Middlebrook and Cohn 7H10 Agar .............................................356Middlebrook OADC Enrichment ..................................................356MIL Medium ...............................................................................322Milk Agar ....................................................................................359Minerals Modified Glutamate Broth ............................................360Minimal Agar Davis .....................................................................362Minimal Broth Davis without Dextrose.........................................362MIO Medium ..............................................................................323Mitis Salivarius Agar ....................................................................364Modified Oxford Antimicrobic Supplement..................................410Modified Thayer-Martin (MTM II) Agar ................................365, 541Moeller Decarboxylase Broth Base ...............................................179Moeller Decarboxylase Broth with Arginine .................................179Moeller Decarboxylase Broth with Lysine .....................................179Moeller Decarboxylase Broth with Ornithine ................................179Moeller Decarboxylase Broths .............................................179, 365Moeller KCN Broth Base ..............................................................365Motility GI Medium .....................................................................366Motility Indole Lysine Sulfide (MILS) Medium ...............................367Motility Indole Ornithine Medium ...............................................323Motility Nitrate (MN) Medium .....................................................368Motility Test Medium ..................................................................369MR-VP Broth ...............................................................................324MR-VP Medium ..........................................................................324Mucate Agar ...............................................................................370Mucate Broth ..............................................................................370Mueller Hinton Agar ...................................................................371Mueller Hinton Agar with 5% Sheep Blood .................................371Mueller Hinton Broth (Not Cation-Adjusted) ................................377Mueller Hinton Chocolate Agar ...................................................375Mueller Hinton II Agar ................................................................371Mueller Hinton II Agar with Tryptophan.......................................376Mueller Hinton II Broth (Cation-Adjusted) ....................................378Mueller Hinton II Broth (Cation-Adjusted)

with 2% Sodium Chloride.......................................................378Muller Kauffmann Tetrathionate Broth Base ................................381

Product Index, cont.


684

Mycobacteria 7H11 Agar ....................................................382, 503Mycobactosel™ L-J Medium.......................................................383Mycological Agar ........................................................................384Mycophil™ Agar ........................................................................384Mycophil™ Agar with Low pH ....................................................384Mycoplasma Broth Base (Frey) .....................................................417Mycoplasma Enrichment w/o Penicillin ........................................417 Mycoplasma Media .....................................................................386Mycoplasma Supplement ............................................................417Mycosel™ Agar ..........................................................................386MYP Agar ...................................................................................327

NNeomycin Blood Agar .................................................................389Neopeptone, Bacto™ ..................................................................389Neutralizing Buffer ......................................................................391New York City (NYC) Medium, Modified .....................................391Niacin Assay Medium ..................................................................392NIH Thioglycollate Broth ......................................................388, 547Nitrate Broth ...............................................................................394Nocardia Differentiation Media ...................................................396Nocardia ID QUAD ......................................................................397Novobiocin Antimicrobic Supplement ..................................202, 465Nutrient Agar ..............................................................................398Nutrient Agar 1.5% ....................................................................399Nutrient Agar with MUG .............................................................400Nutrient Broth .............................................................................402Nutrient Gelatin ..........................................................................402NZCYM Broth .............................................................................388NZY Broth with Thymine .............................................................388NZYM Broth ................................................................................388

OOatmeal Agar .............................................................................406OF Basal Medium ........................................................................404OF Medium with Carbohydrates .................................................404OFPBL Agar ........................................................................405, 415OGYE Agar Base .........................................................................405Orange Serum Agar ....................................................................407Orange Serum Broth Concentrate 10× .......................................407Oxacillin Screen Agar ..................................................................409Oxord Antimicrobic Supplement, Modified ..................................410Oxford Medium Base ..................................................................410

PM-PA-C Agar ..............................................................................412PALCAM Antimicrobic Supplement .............................................414PALCAM Medium Base ...............................................................414Pantothenate Assay Medium .......................................................420Pantothenate Medium AOAC .....................................................422PC Agar ......................................................................................415Peptic Digest Agar ......................................................................424Peptone, Acidicase™ ............................................................40, 126Peptone, Bacto™ ........................................................................424Peptone, Biosate™ .......................................................................76 Peptone UF, Select ......................................................................437Peptone Iron Agar .......................................................................426Peptone Water ............................................................................427Petragnani Medium ....................................................................428Phenol Red Agar Base .................................................................429Phenol Red Broth Base ................................................................431Phenol Red Broth with Carbohydrates .........................................431Phenol Red Mannitol Agar ..........................................................429Phenylalanine Agar .....................................................................433Phenylethyl Alcohol Agar ............................................................434Phenylethyl Alcohol Agar with 5% Sheep Blood ..........................434Phosphate Buffer, pH 7.2 ............................................................435Phosphate Buffered Saline ..........................................................437Phytone™ Peptone .....................................................................437Phytone™ Yeast Extract Agar ......................................................440Plate Count Agar ........................................................................441m Plate Count Broth ...................................................................442Polypeptone™ Peptone .............................................................443Potato Dextrose Agar ..................................................................444Potato Dextrose Broth .................................................................444Potato Flakes Agar ......................................................................447

Potato Flakes Agar with Chloramphenicol and Gentamicin ..........447Potato Flakes CC Agar ................................................................447Potato Infusion Agar ...................................................................447PPLO Agar (Mycoplasma Agar Base) ............................................417PPLO Broth (Mycoplasma Broth Base) ..........................................417PPLO Media (Mycoplasma Media) ...............................................417Presence-Absence Broth ..............................................................448Proteose No. 3 Agar ....................................................................453Proteose Peptone, Bacto™ ..........................................................450Proteose Peptone, BiTek™ ...........................................................450Proteose Peptone No. 2, Bacto™ .................................................450Proteose Peptone No. 3, Bacto™ .................................................450Proteose Peptone No. 4, Bacto™ .................................................450Pseudomonas Agar F ..................................................................454Pseudomonas Agar P ..................................................................454Pseudomonas Isolation Agar .......................................................456Pseudosel™ Agar ...............................................................132, 457Purple Agar Base .........................................................................457Purple Broth Base ........................................................................457Purple Broth with Carbohydrates ................................................457Pyridoxine Y Medium ..................................................................459

RR2A Agar ....................................................................................461Raka-Ray No. 3 Medium .............................................................462Rapid Fermentation Medium .......................................................463Rapid Fermentation Medium with Carbohydrates ........................463Rapid Urea Broth ........................................................................464Rappaport-Vassiliadis (MSRV) Medium Semisolid Modification .....465Rappaport-Vassiliadis R10 Broth ..................................................467Rappaport-Vassiliadis Salmonella (RVS) Soy Broth ........................468Regan-Lowe Charcoal Agar .........................................................470Regan-Lowe Charcoal Agar without Cephalexin ..........................470Reinforced Clostridial Medium ....................................................471Riboflavin Assay Medium ............................................................473Rice Extract Agar .........................................................................475Rogosa SL Agar ...........................................................................476Rogosa SL Broth ..........................................................................476Rose Agar with 5% Sheep Blood ................................................477Rose Bengal Agar Base ................................................................477Rose Bengal Antimicrobic Supplement C .....................................477Rosolic Acid ................................................................................228

SSabouraud Agar, Modified ..........................................................495Sabouraud Brain Heart Infusion Agar ..........................................488Sabouraud Brain Heart Infusion Agar Base ..................................488Sabouraud Brain Heart Infusion Agar with Antimicrobics .............488Sabouraud Dextrose Agar ...........................................................490Sabouraud Dextrose Agar with Antimicrobics ..............................490Sabouraud Dextrose Agar with Lecithin and Polysorbate 80 ........490Sabouraud Dextrose Agar, Emmons ............................................495Sabouraud Dextrose Agar, Emmons, with Antimicrobics ..............495Sabouraud Dextrose Broth ..........................................................490Sabouraud Liquid Broth Modified ................................................496Sabouraud Maltose Agar ...........................................................490Sabouraud Maltose Broth ...........................................................490Sabouraud Media (Low pH) .........................................................490Sabouraud Medium, Fluid ...........................................................490Saline, 0.45% .............................................................................496Saline, Normal ............................................................................496Salmonella Shigella Agar .............................................................485Salt Broth, Modified ....................................................................497SBG Sulfa Enrichment ...................................................................67Schaedler Agar ...........................................................................497Schaedler Agar with Vitamin K1 and 5% Sheep Blood .................497Schaedler Broth ..........................................................................497Schaedler Broth with Vitamin K1 .................................................497Schaedler K-V Agar with 5% Sheep Blood ..................................497Select Phytone™ UF ...................................................................437Select Soytone ............................................................................437Selective Seven H11 Agar ...................................................500, 503Selective Streptococcus Agar .......................................................500Selenite Broth .............................................................................500Selenite Cystine Broth .................................................................501Selenite-F Broth ..........................................................................500


685

Serum Tellurite Agar ....................................................................503Seven H11 Agar ..........................................................................503Seven H11 Agar Base ..................................................................503Seven H11 Agar with Aspartic Acid and Pyruvate ........................503SF Broth ......................................................................................479SF Medium .................................................................................479SFP Agar Base .............................................................................480Shigella Broth .............................................................................506SIM Medium ...............................................................................482Simmons Citrate Agar .................................................................508Skim Milk ....................................................................................509Skim Milk Medium ......................................................................509Skirrows Medium ................................................................120, 510Snyder Test Agar .........................................................................510SOB Medium ..............................................................................483Sodium Hippurate Broth .............................................................511Sorbitol MacConkey Agars ..........................................................512Sorbitol MacConkey II Agar with Cefixime and Tellurite ...............335Soybean-Casein Digest Agar Media .............................................567Soybean-Casein Digest Broth Media ............................................579Soytone ......................................................................................512Soytone, Bacto™ .........................................................................437Special Yeast and Mold Medium .................................................512Spirit Blue Agar ...........................................................................513Spirolate Broth ............................................................................515SPS Agar .....................................................................................484SS Agar .......................................................................................485Standard Methods Agar ......................................................441, 516Standard Methods Agar with Lecithin and Polysorbate 80 ...........516m Staphylococcus Broth ..............................................................517Staphylococcus Medium 110 ......................................................518Starch Agar .........................................................................396, 519Starch Agar with Bromcresol Purple ............................................520Sterility Test Broth ...............................................................521, 547Stock Culture Agar .....................................................................521Strep ID QUAD ............................................................................522Sulfite Agar .................................................................................523Super Broth (Animal Free) ...........................................................526Supplement B .............................................................................242Supplement VX ...........................................................................242SXT Blood Agar ...........................................................................487Synthetic Broth AOAC ................................................................527

TTAT Broth ....................................................................................528TAT Broth Base ............................................................................528TC Lactalbumin Hydrolysate, Bacto™ ...........................................529TC Yeastolate, Bacto™ ................................................................529TC Yeastolate, UF ........................................................................529TCBS Agar ..................................................................................530Tech Agar ...........................................................................454, 537m TEC Agar ................................................................................532mTEC Agar, Modified ..................................................................533Tellurite Glycine Agar ..................................................................537Tellurite Solution 1% ...........................................................364, 537Terrific Broth ...............................................................................539Tetrathionate Broth Base .............................................................540m TGE Broth ...............................................................................584Thayer-Martin (MTM II) Agar, Modified........................................541Thayer-Martin Selective Agar .......................................................541Thermoacidurans Agar ................................................................543Thiamine Assay Medium .............................................................544Thiamine Assay Medium LV .........................................................544Thioglycollate Broth, NIH .............................................................547Thioglycollate Medium, Enriched .................................................547Thioglycollate Medium, Enriched, Fluid .......................................547Thioglycollate Medium, Fluid .......................................................547Thioglycollate Medium (Fluid), without Dextrose

or (Eh) Indicator ......................................................................547Thioglycollate Medium, Brewer Modified ....................................547Thioglycollate Medium with Beef Extract, Fluid ............................547Thioglycollate Medium with Calcium Carbonate .........................547Thioglycollate Medium with Calcium Carbon, Enriched ...............547Thioglycollate Medium without Dextrose ....................................547Thioglycollate Medium without Indicator (135C) .........................547Thiol Broth ..................................................................................553Tinsdale Agar Base ......................................................................554

Tinsdale Enrichment Desiccated ..................................................554Todd Hewitt Broth .......................................................................556Todd Hewitt Broth, Bacto™ .........................................................556Todd Hewitt Broth with Gentamicin and Nalidixic Acid ................556Tomato Juice Agar ......................................................................557Tomato Juice Agar, Special ..........................................................557Tomato Juice Broth .....................................................................557Transport Media ..........................................................................559Transport Medium (Stuart, Toshach and Patsula)..........................559Transport Medium Amies ............................................................559Trichophyton Agars 1 – 7 ............................................................562Trichosel™ Broth, Modified .........................................................564Triple Sugar Iron Agar .................................................................565Tryptic Soy Agar ..........................................................................567Tryptic Soy Agar with Lecithin and Polysorbate 80 .......................571Tryptic Soy Broth .........................................................................579Tryptic Soy Broth without Dextrose, Bacto™ ................................579Tryptic Soy Blood Agar Base EH ...................................................574Tryptic Soy Blood Agar Base No. 2 ...............................................574Trypticase™ Peptone ..........................................................128, 584Trypticase™ Soy Agar (Soybean-Casein Digest Agar) ..................567Trypticase™ Soy Agar, Modified (TSA II) ......................................574Trypticase™ Soy Agar with 5% Horse Blood (TSA II) ...................574Trypticase™ Soy Agar with 5% Rabbit Blood (TSA II) ..................574Trypticase™ Soy Agar with 5% Sheep Blood (TSA II) ...................574Trypticase™ Soy Agar with 5% Sheep Blood (TSA II)

with Ampicillin ........................................................................578Trypticase™ Soy Agar with 5% Sheep Blood (TSA II)

with Gentamicin .....................................................................578Trypticase™ Soy Agar with 10% Sheep Blood (TSA II) .................574Trypticase™ Soy Agar with Lecithin, Polysorbate 80 ....................571Trypticase™ Soy Agar with Lecithin and Polysorbate 80

and Penicillinase......................................................................571Trypticase™ Soy Agar with Penicillinase ......................................571Trypticase™ Soy Broth ................................................................579Trypticase™ Soy Broth with 0.15% Agar ....................................583Trypticase™ Soy Broth with 5% Fildes Enrichment ......................579Trypticase™ Soy Broth with 20% Glycerol ..................................584Trypticase™ Soy Broth with 6.5% Sodium Choride .....................579Tryptone ............................................................................128, 584Tryptone, Bacto™ ........................................................................128Tryptone, BiTek™ ........................................................................128Tryptone Glucose Extract Agar ....................................................584Tryptone Glucose Yeast Agar .......................................................441Tryptone Water ...........................................................................586Tryptose Agar .............................................................................588Tryptose, Bacto™ ........................................................................587Tryptose Blood Agar Base ............................................................590Tryptose Broth .............................................................................588Tryptose Phosphate Broth, Bacto™ ..............................................592TSA Blood Agars .........................................................................574TSI Agar ......................................................................................565TSN Agar ....................................................................................535TT Broth Base, Hajna ...................................................................536TTC Solution 1% ........................................................................275Tween™ 80 Water .......................................................................593Tyrosine Agar ......................................................................396, 593

UUniversal Beer Agar .....................................................................595Universal Preenrichment Broth ....................................................596Urea Agar ...................................................................................597Urea Agar Base ...........................................................................597Urea Agar Base Concentrate 10× ...............................................597Urea Broth ..................................................................................597Urease Broth Concentrate 10× ...................................................597Urease Test Broth ........................................................................597UVM Modified Listeria Enrichment Broth .....................................594

VV Agar ........................................................................................600Vancomycin Screen Agar .............................................................602VCA Inhibitor ..............................................................................242VCAT Inhibitor ............................................................................242VCN Inhibitor ..............................................................................242VCNT Inhibitor ............................................................................242Veal Infusion Agar .......................................................................603

Product Index, cont.


686

Veal Infusion Broth ......................................................................603Violet Red Bile Agar ....................................................................604Violet Red Bile Agar with MUG ...................................................606Violet Red Bile Glucose Agar .......................................................607Vitamin B12 Assay Medium ..........................................................609Vitamin K1 - Hemin Solution ........................................................611VJ Agar (Vogel and Johnson Agar) ..............................................601Vogel and Johnson Agar .....................................................601, 612

WWallenstein Medium ...................................................................614Water .........................................................................................614Wilkins-Chalgren Agar ................................................................615WL Differential Medium ..............................................................612WL Nutrient Broth .......................................................................612WL Nutrient Medium ..................................................................612

XXanthine Agar ....................................................................396, 621XL Agar Base ..............................................................................616XLD Agar ....................................................................................616XLT4 Agar Base ...........................................................................620XLT4 Agar Supplement ...............................................................620

Y2× Yeast Extract Tryptone (2×YT) Medium .................................626Yeast Carbon Base ......................................................................628Yeast Extract ...............................................................................621Yeast Extract, Bacto™ ..................................................................621Yeast Extract Glucose Chloramphenicol Agar ..............................623Yeast Extract, LD .........................................................................621Yeast Extract-Peptone-Dextrose (YPD) Agar .................................624Yeast Extract-Peptone-Dextrose (YPD) Broth ................................624Yeast Extract Phosphate (YEP) Agar .............................................625Yeast Extract, Technical, Bacto™ ..................................................621Yeast Extract, UF .........................................................................621Yeast Fermentation Broth Base with Durham Tube ......................627Yeast Fermentation Broth with Carbohydrates

and Durham Tube ...................................................................627Yeast Mold (YM) Agar .................................................................632Yeast Mold (YM) Broth ................................................................632 Yeast Morphology Agar ..............................................................628Yeast Nitrogen Agar ....................................................................628Yeast Nitrogen Base ....................................................................628Yeast Nitrogen Base without Amino Acids ...................................628Yeast Nitrogen Base without Amino Acids

and Ammonium Sulfate ..........................................................628Yersinia Antimicrobic Supplement CN .................................113, 633Yersinia Selective Agar Base ................................................113, 633YPD Agar ....................................................................................621


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