Different Kinds of Microscopes

Learning how to use the microscopeLearning how to use the microscope

Click link below to watch video:

There are 2 kinds of microscopes:

Simple: one lensone lens (magnifyingmagnifying glass)

Compound: 2 or more lenses

Watch this video clip:Simple vs. Compound Video Clip

Parts of the MicroscopeParts of the Microscope

1. Revolving Revolving NosepieceNosepiece

Parts of the MicroscopeParts of the Microscope

2. 2. Lens/ObjectiLens/Objectiveve

Parts of the MicroscopeParts of the Microscope

3. 3. StageStage

Parts of the MicroscopeParts of the Microscope

4. 4. Disc Disc DiaphragmDiaphragm

(controls amount (controls amount of of lightlight))

1 = 1 = lowestlowest setting/setting/

55 = = highest settinghighest setting

Parts of the MicroscopeParts of the Microscope

5. 5. Light Light sourcesource

Parts of the MicroscopeParts of the Microscope

6. 6. BaseBase

Parts of the MicroscopeParts of the Microscope

7. 7. EyepieceEyepiece

Parts of the MicroscopeParts of the Microscope

8. 8. ArmArm

Parts of the MicroscopeParts of the Microscope

9. 9. Stage ClipsStage Clips

Parts of the MicroscopeParts of the Microscope

10. 10. Coarse Coarse Focus/Focus/

Adjustment Adjustment

(Big Knob)(Big Knob)

Parts of the MicroscopeParts of the Microscope

11. 11. Fine Fine Focus/Focus/

Adjustment Adjustment

(Small Knob)(Small Knob)

Calculating Calculating MagnificationMagnification

MultiplyMultiply lens powers together! lens powers together!

• EyepieceEyepiece: 10 10 XX by itself

• Low Power: Low Power: 44X; redred color; and a TOTAL magnification of 40 X40 X mainly used for large objects/scanninglarge objects/scanning

• Medium Power: Medium Power: 1010 X, X, yellowyellow color; TOTAL magnification of 100 X100 X

• High Power: High Power: 4040 X; X; blueblue color; and a TOTAL magnification of 400 X400 X; ; mainly used for mainly used for smaller smaller objects/detailsobjects/details

Other Things to Other Things to Consider:Consider:

Carrying the Microscope: Always use Always use 2 hands!2 hands!

Watch this Video Clip: Parts of the Microscope

When you are ready to When you are ready to start:start:

Make sure it is on LOWLOW power!

The stage should be all the way downdown (you focus by slowly moving it up!)

Check the light setting (should be at 1-2 to 1-2 to startstart)

Other Things to Other Things to Consider:Consider:

Never use the COARSE FOCUS KNOB while on HIGHHIGH power.

TROUBLESHOOTINGTROUBLESHOOTINGWhat if you can’t get your object What if you can’t get your object

into focus?into focus?

•Check that you have one of the lenses clicked into place

•Check that your disc diaphragm is in place (not IN IN BETWEENBETWEEN settings)

TROUBLESHOOTINGTROUBLESHOOTINGWhat if you can’t get your object What if you can’t get your object

into focus?into focus?

• Clean off your lenses using LENS PAPERLENS PAPER. (Also, dirty slides are hard to focus on; so clean them, too!)

• GO BACK TO LOW POWER AND START OVER!

When putting the When putting the microscope away (at the microscope away (at the

end of the day):end of the day):It should be on LOWLOW power.The cord should be WRAPPEDWRAPPED

around the base.The stage should be all the

way DOWNDOWN (do this with the coarse focus knob!)

The light switch should be OFFOFF

• Use a MAGNETICMAGNETIC field to bend beams of light; used to see things at much higher level of detail

• Can magnify images up to 1,000,0001,000,000 times!

There are 2 kinds:

• SCANNING ELECTRON MICROSCOPES (SEM): Allows you to see the SURFACESSURFACES of objects

There are 2 kinds:

• TRANSMISSION ELECTRON MICROSCOPES (TEM): Allow you to see THROUGHTHROUGH an object.

Electron Microscope ImagesElectron Microscope Images

Go to this website and check out the cool images! Answer the 2 questions on your notes page.

http://www.pbrc.hawaii.edu/bemf/microangela/