1 PhD Program in Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 2 Division of Operative Dentistry, Department of General Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 3 Division of Pediatric Dentistry, Department of Orthodontics and Pediatric Dentistry, University of Maryland School of Dentistry, Baltimore, MD 21201, USA 4 Department of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USA
Dimensional Stability of Impression Materials Disinfected by COEfectTM MinuteSpray
Kuei-Ling C. Hsu1,3, Louis DePaola4, Mary Anne S. Melo1,2
• Disinfection of clinical infected materials is a part of important and necessary
steps for proper infection control protocol in dentistry.
• Spray surface disinfectant is commonly used to disinfect impression of fixed
and removable prosthesis. A few commercial surface disinfectants are
available to use in dental impression. However, the effect of these surface
disinfectant in dimensional and morphological changes are yet to be
determined. Example of the content in these disinfectants is listed below.
• The purpose of this study was to determine the effect of commercially available
disinfectant solutions on the dimensional stability and surface quality of two
types of impression materials available to clinicians: dental alginate and
polyvinyl siloxane impression materials.
INTRODUCTION
MATERIALS AND METHODS
RESULTS
CONCLUSION
• Two types of impression materials were evaluated: alginate and polyvinyl
siloxane (PVS).
• Four one-step spray antimicrobial disinfectants (BirexSE®, Opti-Cide3®, COEfect™ MinuteSpray, CaviCide ) along with control (distilled water) were used
to disinfect the edentulous impression by both materials according to the
manufacture instruction. Procedures were repeated in triplicates.
• To evaluate the dimensional stability, the impression was scanned before and
after disinfectant spray by a CBCT scanner (Carestream 9300, Atlanta, GA)
and revered to a 3D positive image using related Kodak software. Linear
measurement were obtained on the digital model using 3 fixed reference
points. ANOVA one-way and Tukey test were used to analyze the difference
of linear changes for each disinfectant. Significant level was set at p<0.05.
• To evaluate the surface quality of the impression, a representative specimen
of each group were prepared and examined with electronic scanning
microscopy (SEM). All specimens were examined under an FEI Quanta 200
operating at 25kV and X500 magnification.
Fig. 1: Localization and demarcation ofreference metallic points on maxillarycomplete edentulous template.
• Disinfection of the impressions by the tested commercially available disinfectant solutions did not yield significantlinear dimensional changes in dimensions and morphological alteration of the used dental alginate and polyvinylsiloxane impression material.
• The disinfection of impression materials frequently used in the dental clinical procedures such alginate and polyvinylsiloxane impression material can be performed without detrimental to the dimensional stability of the material.
Fig. 5: Mean and standarddeviation of dimensional differencesof dental alginate subjected todisinfection with differentdisinfecting solutions. The horizontalline indicates that all five groups hada similar dimensional difference (p >0.5).
Fig. 6: Mean and standarddeviation of dimensional differencesof polyvinyl siloxane impressionmaterial subjected to disinfectionwith different disinfecting solutions.The horizontal line indicates that allfive groups had a similardimensional difference (p > 0.5).
Fig. 7: SEM micrograph ofdental alginate and polyvinylsiloxane impression materialsubjected to water (control group)and COEfect™ MinuteSpray ,respectively, presenting nomorphological alterations(×500).
ACKNOWLEDGEMENTS
• This project is supported by GC America Inc. USA.
Fig. 2: Trays loaded with dental alginate impression before the disinfectionprocedures.
Fig. 3: Impressions were scannedimmediately after impression-takingwith a CBCT scanner (Carestream 9300,Atlanta, GA)
Fig. 4: Fabrication of a digital dental model from the CBCT scan ofimpressions: A: the impression was scanned by CBCT; the negative form ofthe image was changed into positive form via software 3D imagereconstruction. B: linear measurements were made on the digital models inthe A-B, A-C and B-C dimensions.
Dimensional Stability
Surface Quality
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