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DNA Chapter 16. Griffith - 1928 Streptococcus pneumoniae - bacteria that causes pneumonia in mammals...

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DNA Chapter 16 Slide 2 Griffith - 1928 Streptococcus pneumoniae - bacteria that causes pneumonia in mammals R strain harmless S strain pathogenic mixed heat-killed S strain with live R strain bacteria and injected this into a mouse the mice died Slide 3 Transformation change in genotype and phenotype due to the assimilation of a foreign substance (now known to be DNA) by a cell. Slide 4 Oswald Avery 1944 World knows a molecule carries the genetic information. World knows a molecule carries the genetic information. Doesnt know if the molecule is a: protein, lipid, carbohydrate, RNA, or DNA Doesnt know if the molecule is a: protein, lipid, carbohydrate, RNA, or DNA Avery performs Griffiths experiment again with a twist. Avery performs Griffiths experiment again with a twist. Avery and other scientists discovered that DNA is the nucleic acid that stores and transmits the genetic information from one generation of an organism to the next. Slide 5 What is the transforming substance? Track the infection of bacteria by viruses Viruses consist of a DNA enclosed by a protective coat of protein To replicate, a virus infects a host cell and takes over the cells metabolic machinery Slide 6 Bacteriophages (phage) Virus that specifically attacks bacteria Slide 7 Hershey and Chase T2 phage, consisting almost entirely of DNA and protein, attacks Escherichia coli label protein and DNA and then track which entered the E. coli cell during infection Slide 8 How? Grew one batch of T2 phage in the presence of radioactive sulfur marking the proteins but not DNA Grew another batch in the presence of radioactive phosphorus marking the DNA but not proteins Allowed each batch to infect separate E. coli cultures Slide 9 Slide 10 Chargaff Developed a series of rules based on a survey of DNA composition in organisms DNA is a polymer of nucleotides consists of a nitrogenous base, deoxyribose, and a phosphate group The bases = adenine (A), thymine (T), guanine (G), or cytosine (C). The four bases are found in ratios Slide 11 Wilkins and Franklin X-rays are diffracted as they passed through aligned fibers of purified DNA Used to deduce the three- dimensional shape of molecules Slide 12 Watson and Crick Double Helix Slide 13 Purine and Pyrimidine nitrogenous bases are paired in specific combinations: adenine with thymine and guanine with cytosine Slide 14 DNA Replication Because each strand is complementary to each other, each can form a template when separated Slide 15 DNA Replication Slide 16 semiconservative replication each of the daughter molecules will have one old strand and one newly made strand Slide 17 DNA replication http://www.johnkyrk.com/DNAreplication. html http://www.johnkyrk.com/DNAreplication. html Slide 18 Human Cells Copy its billion base pairs and divide into daughter cells in a few hours One error per billion nucleotides Slide 19 Origin of Replication Single specific sequence of nucleotides that is recognized by replication enzymes enzymes separate the strands, forming a replication bubble Replication proceeds in both directions until the entire molecule is copied Slide 20 Anti-Parallel Each DNA strand has a 3 end with a free hydroxyl group attached to deoxyribose and a 5 end with a free phosphate group attached to deoxyribose. Slide 21 Bubbles and Forks Slide 22 DNA Polymerase Slide 23 DNA polymerases can only add nucleotides to the free 3 end of a growing DNA strand leading strand - used by polymerases as a template for a continuous complimentary strand lagging strand - copied away from the fork in short segments (Okazaki fragments) Slide 24 DNA polymerase and Primers Polymerase cannot initiate synthesis of a polynucleotide can only add nucleotides to the end of an existing chain To start a new chain requires a primer a short segment of RNA Slide 25 Replication Fork - Topoisomerases - Helicases - Single-strand binding proteins - Primases (RNA primers) - DNA Polymerases - Ligases Animation: Leading Strand Animation: Leading Strand Animation: Lagging Strand Animation: Lagging Strand Slide 26 DNA polymerase later replaces the primer with deoxyribonucleotides complimentary to the template Animation: DNA Replication Review Animation: DNA Replication Review Slide 27 Slide 28 Mismatched nucleotides Reactive chemicals, radioactive emissions, X-rays, and ultraviolet light can change nucleotides in ways that can affect encoded genetic information Each cell continually monitors and repairs its genetic material over 130 repair enzymes identified in humans Slide 29 In mismatch repair, special enzymes fix incorrectly paired nucleotides In nucleotide excision repair, a nuclease cuts out a segment of a damaged strand Slide 30 telomeres = The ends of eukaryotic chromosomal DNA molecules long repetitive sequences (no genes) Slide 31 Telomerase uses a short molecule of RNA as a template to extend the 3 end of the telomere Slide 32 Difference between bacterial chromosomes and eukaryotic chromososes Slide 33 Chromatin is a complex of DNA and protein, and is found in the nucleus of eukaryotic cells Chromatin is a complex of DNA and protein, and is found in the nucleus of eukaryotic cells Histones are proteins that are responsible for the first level of DNA packing in chromatin Histones are proteins that are responsible for the first level of DNA packing in chromatin Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Animation: DNA Packing Animation: DNA Packing Slide 34 Fig. 16-21a DNA double helix (2 nm in diameter) Nucleosome (10 nm in diameter) Histones Histone tail H1 DNA, the double helixHistones Nucleosomes, or beads on a string (10-nm fiber) Slide 35 Fig. 16-21b 30-nm fiber Chromatid (700 nm) LoopsScaffold 300-nm fiber Replicated chromosome (1,400 nm) 30-nm fiber Looped domains (300-nm fiber) Metaphase chromosome Slide 36 Chromatin is organized into fibers Chromatin is organized into fibers 10-nm fiber 10-nm fiber DNA winds around histones to form nucleosome beads DNA winds around histones to form nucleosome beads Nucleosomes are strung together like beads on a string by linker DNA Nucleosomes are strung together like beads on a string by linker DNA 30-nm fiber 30-nm fiber Interactions between nucleosomes cause the thin fiber to coil or fold into this thicker fiber Interactions between nucleosomes cause the thin fiber to coil or fold into this thicker fiber Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Slide 37 300-nm fiber 300-nm fiber The 30-nm fiber forms looped domains that attach to proteins The 30-nm fiber forms looped domains that attach to proteins Metaphase chromosome Metaphase chromosome The looped domains coil further The looped domains coil further The width of a chromatid is 700 nm The width of a chromatid is 700 nm Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Slide 38 Most chromatin is loosely packed in the nucleus during interphase and condenses prior to mitosis Most chromatin is loosely packed in the nucleus during interphase and condenses prior to mitosis Loosely packed chromatin is called euchromatin Loosely packed chromatin is called euchromatin During interphase a few regions of chromatin (centromeres and telomeres) are highly condensed into heterochromatin During interphase a few regions of chromatin (centromeres and telomeres) are highly condensed into heterochromatin Dense packing of the heterochromatin makes it difficult for the cell to express genetic information coded in these regions Dense packing of the heterochromatin makes it difficult for the cell to express genetic information coded in these regions Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings Slide 39 Histones can undergo chemical modifications that result in changes in chromatin organization Histones can undergo chemical modifications that result in changes in chromatin organization For example, phosphorylation of a specific amino acid on a histone tail affects chromosomal behavior during meiosis For example, phosphorylation of a specific amino acid on a histone tail affects chromosomal behavior during meiosis Copyright 2008 Pearson Education Inc., publishing as Pearson Benjamin Cummings

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