DO NOW: 1. Take out your microscope YELLOW packets from last

week.2. In your NOTES section of your binder, please list EIGHT

differences between these two pictures.

Stare at the red dot on the girl’s nose for 30 seconds.Turn your eyes to a plain surface.Blink repeatedly and quickly.

Using a Compound Light Microscope Directed Reading

Using the Microscope1.) The magnifies the image x.

2.) The low- magnifies the image x.

3.) The high- magnifies the image either x or x.

ocular lens

10

power objective10

power objective40 43

Using the Microscope4.) The holds the objective and can be turned to change from one

to the other.

5.) The maintains the correct distance between the and . 

6.) The moves the body tube and down to allow focusing of the

.

revolving nosepiece magnification

body tube ocular

lensobjectives

coarse - adjustment knobup image

Using the Microscope

7.) The moves the body tube to bring the image into focus. 8.) The supports a slide.

9.) hold the slide in place for .

fine- adjustment knob slightly sharper

stage

Stage clipsviewing

Using the Microscope10.) The controls that amount of coming through the stage.

11.) The light sources provides a for viewing the .

12.) The supports the body .

diaphragmlight

slidelight

arm tube

Using the Microscope13.) The supports the .

14.) Inclination joint (hinge): Allows the upper part of the microscope to be tilted back to view the specimen while seated.

base microscope

Proper Use of the Compound Light

Microscope1.) How would you carry a microscope?

a) With your left handb) With your right handc) With both hands

2.) How many centimeters should the microscope be from the edge of a table?

a) 1 cmb) 5 cmc) 10 cm

Proper Use of the Compound Light

Microscope

3.)  What power objective should you ALWAYS begin working with?

a) Lowb) Mediumc) High

Proper Use of the Compound Light

Microscope

4.)  What must be adjusted to get the microscope into low power?

a) Revolving objectiveb) Revolving nosepiecec) Revolving ocular lens

Proper Use of the Compound Light

Microscope

5.) Once you place a prepared slide over the hole in the stage, what secures the slide?

a) A rubber bandb) Your fingerc) Stage clips

Proper Use of the Compound Light

Microscope

6.) If the diaphragm is moved, what gets adjusted?

a) The height of the stageb) The amount of lightc) The objective lens

Proper Use of the Compound Light

Microscope

7.) Once you are looking at the stage from eye level, you should slowly turn the:

a) Fine adjustment knobb) Medium adjustment knobc) Course adjustment knob

Proper Use of the Compound Light

Microscope

8.) You should NEVER allow the objective to touch the slide

a) Trueb) Falsec) Sometimes it can

Proper Use of the Compound Light

Microscope

9.) While looking through the ocular lens, you should turn the coarse adjustment knob to raise the low-power objective so that the image is:

a) Focusedb) Blurredc) Not visible

Proper Use of the Compound Light

Microscope

10.) Once the object is focused, what sharpens the image?

a) Fine adjustment knobb) Medium adjustment knobc) Coarse adjustment knob

Proper Use of the Compound Light

Microscope

11.)  When you are in high power you should always use the coarse adjustment knob?

a) Trueb) Falsec) Sometimes

Proper Use of the Compound Light

Microscope

12.) When you are finished using the microscope, you should remove the slide and clean the ocular and objective lens with what type of paper?

a) Lens paperb) Sand paperc) Construction paper

Proper Use of the Compound Light

Microscope

13.) When you are all finished using the microscope, you should always:

a) Return it to the storage areab) Leave it where it isc) Throw it away

Proper Use of the Compound Light

Microscope

Making a Wet Mount Slide1.) Use sand paper to clean a glass slide and a coverslip.

LENS

2.)  Place the specimen you wish to observe on the left of the slide.

Making a Wet Mount Slide

CENTER

3.)  Using a medicine dropper, place 5 drops of water on the specimen.

Making a Wet Mount Slide

ONE

4.)  Hold the coverslip at the edge of the water at a 90 degree angle to the slide. Make sure the water runs along the middle of the coverslip.

Making a Wet Mount Slide

45 & EDGE

5.)  Lower the coverslip quickly to avoid trapping air bubbles.

Making a Wet Mount Slide

SLOWLY

6.)  Water might evaporate from the slide as you work. Remove more water to keep the specimen fresh.

Making a Wet Mount Slide

ADD

6a.)  Place the tip of the medicine dropper next to edge of the coverslip. Add 4 drops of water.

Making a Wet Mount Slide

A (ONE)

6b.)  Remove excess water from the slide by using the center of a paper towel as a blotter.

Making a Wet Mount Slide

CORNER

6c.)  Lift the coverslip to add or remove water.

Making a Wet Mount Slide

DO NOT