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Your Reliable Source of Human Primary Cells Donor HLA Typing 101: How HLA Characterization Can Accelerate Your Research
Your Reliable Source of Human Primary Cells
Donor HLA Typing 101:How HLA Characterization Can Accelerate Your Research
HLA – What is it?
HLA typing technology
Applications
Q&A
1
2
3
4
Macrophage or APC
B cells
foreign protein
peptide / HLA
T cells
Activated T cells
60-sec Immunology Review
ER
Major Histocompatibility
Complex (MHC)
Antigen-specific T cell
T cell Receptor (TCR)
Human Leukocyte Antigen (HLA)
HLA molecules “present” peptide to T cells
CD4 T helper cell
HLA Class IIHLA Class I
CD8+ Cytotoxic T cell
Cytosolic proteins
Proteosome
All nucleated cells
Extracellular pathogens
(except cross-presentation)
Lysosome
Only APCs
13-25 aa 9-22 aa
2 flavors of HLA
CLA
SS I
1
CLA
SS II
HLA diversity is the result of genetic polymorphisms
HLA-B8
*0801*0802*0803
….. *0821
HLA- B > 3,000 known alleles
• Crossmatch
• Microcytotoxicity Test
• Mixed Lymphocyte Reaction Assay
• Immunophenotyping
• SSP Genotyping
• SSO Genotyping
Evolution of HLA Typing
SSO Genotyping Method
HLA Data Analysis
Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Vaccine Development
HLA and Autoimmune disease
Disease HLA Allele
Celiac Disease HLA-DQ8, -DQ2
Type I Diabetes HLA-DRB1*04:01
Multiple Sclerosis HLA-DRB1*15:01
RA (ankylosingspondylitis)
B27
Behçet's disease HLA-B51
Transplantation
Forensics
Disease-related Biomarkers
HLA-typing applications
Immunogenicity
Vaccine Development
HLA and Immunogenicity
• HLA-B*57:01
Ostrov et al, PNAS, 2012
AllCells HLA-typed
Primary Cells*AllCells
Immunoassays
PBMC
T cells
B cells monocytes
Cytokine Release Assays
MLR Assays
T cell proliferation
T helper cell polarization
HLA typing at AllCells
* Primary cells from healthy donors or diseased patients
Bruce Sun
Sr. Manager, Product and Application Development
Please direct offline questions to [email protected] or
www.allcells.com/technical-support/
www.allcells.com/support/webinars/
Contact Information
