DOSSIER FMD epidemiological situation in...

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MINISTRY OF AGRICULTURE AND FOOD

BULGARIAN FOOD SAFETY AGENCY

* Sofia, 1606, “Pencho Slaveikov” blvd. 15( +359 (0) 2 915 98 20, +359 (0) 2 954 95 93, www.bfsa.government.bg

DOSSIER

FMD epidemiological situation in Bulgaria

Application by the Bulgarian Food Safety Agencyfor recovery of the former status of whole theritory of Bulgaria as a “COUNTRY FREE FROM FMD WITHOUT VACCINATION”.

Sofia, Bulgaria14/11/2011

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Content

1. FMD eradication ………………………………………………………………………………………………… 32. FMD surveillance. Surveillance strategy and measures applied in relation with all FMD

outbreaks…………………………………………………………………………………………………………… 183. Epidemiological findings and considerationsin relation with all outbreaks

……………………………………………………………………………………………………………………… …204. Next steps……………………………………………………………………………………… … 215. Annexes:

- Annex I - surveillance in 3km protective and 10 km surveillance zones of the outbreak Kost (IP-1)i,Tsarevo municipality,Burgas region………………………………………………… 25

- Annex II - surveillance in 3km protective and 10 km surveillance zones of the outbreak Rezovo (IP-2), Tsarevo municipality, Burgas region……………………………………… ……………29

- Annex III - surveillance in 3km protective and 10 km surveillance zones of the outbreakGramatikovo(IP-3),Malko Turnovo municipality, Burgas region…………………… ..32

- Annex IV - surveillnace in the settlements of Burgas region outside the protection surveillance zonesaround the outbreaks - time period – 05.01-27.02.2011………… …………35

- Annex V - surveillnace in 3km protective and 10 km surveillance zones of the outbreak Kirovo(IP-4),Sredetc municipality, Burgas region - second outbreak wave……………… ….52

- Annex VI - surveillnace in 3km protective and 10 km surveillance zones of the outbreakGranichar(IP-5), Sredetc municipality, Burgas region - second outbreak wave……… 54

- Annex VII - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks GolyamoBukovo(IP-6), Fakiya(IP-7) and Momina Tsurkva(IP-8), Sredets municipality, Burgas region -second outbreak wave…………………………………………………………… 56

- Annex VIII - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks Bliznakvillage (IP-9) and Bliznak buffalo farm (IP-10), Malko Turnovo municipality, Burgas region - secondoutbreak wave…………………………………………………………… 59

- Annex IX - surveillnace in 3km protective and 10 km surveillance zones of the outbreaks DolnoYabalkovo(IP-11),Sredets municipality Burgas region - second outbreak wave… 61

- Annex X - surveillance in the framework of disease surveillance after the last outbreaks– time period18.04-19.04.2011……………………………………………………………………… .. 66

- Annex XI - surveillance in the framework of disease surveillance after the last outbreaks– time period28 - 29 .04.2011…………………………………………………………… 75

- Annex XII - surveillance in the framework of disease surveillance after the last outbreaks– timeperiod 18 -19.05.2011…………………………………………………………… 79

- Annex XIII - surveillance in the framework of disease surveillance after the last outbreaks– timeperiod 16 - 25.08.2011…………………………………………………………… 85

- Annex XIV - surveillance in wild animals - time period January – August 2011……… 97- Annex XV - time of FMD events in Bulgaria 2010 – 2011……………………………… 99

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1. FMD eradication.

a) History. Chronology of the FMD cases and outbreaks in the country.

Ø On 5th of January 2011 -date of first case of FMD in one wild boar shot on 30December 2010 about 2 km north from the Turkish border in the vicinity of the villageof Kosti (Tsarevo municipality, Burgas region).

Ø On 9th of January in the village of Kosti (IP-1), Tsarevo municipality, in the vicinityof the place were the wild boar was shot;

Ø On 15th of January in the village of Rezovo (IP-2), Tsarevo municipality, locatedsoutheast of Kosti at the border with Turkey;

Ø On 31st of January in the village Gramatikovo (IP-3), Malko Tarnovo municipality,situated 10 km west of Kosti within the surveillance zone around IP-1 in Kosti;

Ø On 19th of March in a bovine farm nearby the village of Kirovo (IP-4), Sredetsmunicipality, 7 km north of the border with Turkey;

Ø On 24th of March in the village of Granichar (IP-5),Sredets municipality, 3 km northof the border with Turkey;

Ø On 25th of Marcho in a mixed bovine and pig farm nearby the village of Goliamo Bukovo (IP-6),

Sredets municipality, 5 km northeast of IP-4, within the surveillance zonearound the Kirovo outbreak;

o in a bovine farm nearby the village of Fakia (IP-7), Sredets municipality, westof the Kirovo outbreak, 10 km north of the border with Turkey;

o in a bovine farm nearby the village of Momina Tsarkva (IP-8), Sredetsmunicipality west of IP-7, 6 km north of the border with Turkey;

Ø On 3rd of Aprilo in the village of Bliznak (IP-9), Malko Tarnovo municipality 7 km north of the

border with Turkey;

o in a grazing buffalo herd 2 km south of Bliznak village (IP-10), MalkoTarnovo municipality in about 5 km north of the border with Turkey;

Ø On 7th of April in Dolno Yabalkovo (IP-11), Sredets municipality in village herd ofsheep, goats and a bovine.

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107 64

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WB

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TurkeyGreece

Bulgaria Black sea

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Figure 1. (a) WB - wild boar shot 30.12.2010(b) Location of the FMD outbreaks in Burgas region : 1. Kosti; 2. Rezovo; 3. Gramatikovo, 4.

Kirovo; 5. Granichar; 6. Goliamo Bukovo; 7. Fakia; 8. Momina Tsarkva; 9. Bliznak; 10. Herd2 km from Bliznak; 11. Dolno Yabalkovo

b) Chronology of the events, strategy and applied measures related to the control anderadication of FMD in all outbreaks

05.01.2011On 30.12.2010 three wild boars were shot 2 km north from the Turkish border in the area ofMakevtci, on the land of Kosti village (Tsarevo municipality, Burgas region) (see Figure 1).Acute clinical signs for FMD in the feet (coronary band of claws) only in one of the 3 wildboar have been observed. Samples ( tissue samples) from the 3 wild boar were tested on4.01.2011 by lateral flow device for FMDV-antigen detection, Ag ELISA- type O and RTPCR-real time

Ø The samples only from one of tested wild boar showed positive results, asfollow:Lateral flow device for FMDV-antigen detection- positive;

Ø Ag ELISA- type O - positive;Ø RT PCR-real time- protocols by Scott Read and Kalahan- positive for presence of the

FMD viral genome.

On 5 January 2011 the first case of FMD in one wild boar was declared.

On 07.01.2011 the samples were sent to of the EU Reference Laboratory (EU-RL), Institutefor Animal Health, Pirbright, United Kingdom, for confirmation and isolation virus. Theanalysis made from the samples (WB)indicate this FMD virus is a serotype O, Topotype ME-SA, Strain PanAsia-2ANT-10 which has more than 99.5% affinity with isolates collected in2010 in Iran and Turkey.

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05.01.2011Start the implementation of the measures for control and eradication of FMD according toCouncil Directive 2003/85/EC.

Portection (3km) and surveillance (10 km) zones were established around Kosti and clinicaland epidemiological examinations as well as blood sampling of susceptible animals in allvillages located in the municipalities of Tsarevo, Malko Tarnovo and Sredets. (samples intables in Annex II).

08.01.2011By COMMISSION DECISION 2011/8/EU of 6 January 2011 concerning certain interimprotection measures against foot-and-mouth disease in Bulgaria, some regions of the countrywere separated as a zone with high risk Annex I (the entire region of Burgas) and zones withlow risk Annex II (the regions of Yambol, Sliven, Shumen and Varna). No susceptibleanimals were allowed to move from these zones to other territories of Bulgaria. No FMDsusceptible animals and products thereof could leave the territories of Annex I.

Decision 2011/8/EU

Figure 2 Annex I and Annex II areas accroding to Decision 2011/8/EU

09.01.2011

On 09.01.2011 the National Reference laboratory (NRL) for FMD confirmed positive forNSP ELISA and antibodies FMD type O ELISA blood samples taken on 07-08.01.2011during surveillance activities (Figure 4 (b) in animals from Kosti (Tsarevo municipality,Burgas region).The results are as follow:

Cattle - 1 positive for NSP ELISA and antibodies FMD type O ELISASheep – 14 positives for NSP ELISA and antibodies FMD type O ELISAGoats – 12 positives for NSP ELISA tests and antibodies FMD type O ELISAPigs – 8 positives for NSP ELISA

The Veterinary service (National Veterinary Service (NVS) started the implementation of themeasures for the control and eradication of FMD according to Council Directive 2003/85/EC,namely:

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- On 10.01.2011 by Order of the Director General of NVS, establishment of the 3 kmprotective and 10 km surveillance zones around Kosti village and the relevant restrictivemeasures were imposed.By Order of the Executive Director of the Bulgarian Food Safety Agency (BFSA)(BFSA is a newly established agency, in operation since March 2011, wich included allstructures of the NVS) on 04.07.2011 the 3 km protective and 10 km surveillance zoneswere removed.

- On 10.01.2011 by Order of the Director General of NVS, NVS started killing of allsusceptible animals in the village of Kosti. The killing of animals took 3 days, becauseof difficulties to round up 2 free range cattle herds.

- Cleaning and disinfection on the spot. After killing of animals. The empty farms, animalholdings, backyards, roads, pastures were disinfected several times.

- On 10.01.2011 start of the clinical examination and blood sampling in the village in the3 km protective and 10 km surveillance zones around Kosti.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages, and the serological surveillance are listed in Annex I

15.01.2011Since the confirmation of FMD in wild boar on 05.01.11 NVS started clinical andepidemiological examinations as well as blood sampling of susceptible animals in all villageslocated in the municipalities of Tsarevo, Malko Tarnovo and Sredets in Burgas region. Duringthe clinical examinations under the surveillance a suspicion of FMD in Rezovo(Tsarevomunicipality, Burgas region) (Figure 4 (2)) was detected on 15.01.2011.

NVS started the measures in relation with the suspicion according the Directive 2003/85 on15.01.2011.Clinical sings for FMD were observed in cattle and the laboratory resultsconfirmed the disease on 17.01.2011, as follow:

Cattle - 19 samples positive for RT-PCR, 12 positive for Ag ELISA FMDPigs - 3 positive Ag ELISA FMD

After the confirmation of FMD in Rezovo on 17.01.2011 NVS started the implementation ofthe measures for control and eradication of FMD according to Council Directive 2003/85/EC,namely:- On 17.01.2011 by Order of the Director General of NVS 3 km protective and 10 km

surveillance zones were defined around Rezovo village and the relevant restrictivemeasures were imposed. By Order of the Executive Director of BFSA on 04.07.2011 the3 km protective and 10 km surveillance zones were removed.

- On 17.01.2011 by Order of the Director General of NVS, NVS started killing of allsusceptible animals in the village of Rezovo. The killing of animals took 2 days becauseof difficulties to round up free range cattle .

- Furthermore, on the grazing grounds of Rezovo a herd of 21 Turkish buffalos werefound. The buffalos entered into Bulgaria in the beginning of January (4.01.2011) andwere grazing together with the cattle from Rezovo. All 21 buffaloes were killed on20.01.2011 and lesions of FMD were observed during the killing in one of the animal(yonger than 12 months of age, without eartag).

- Cleaning and disinfection on the spot. After killing of the animals the empty farms,animal holdings, backyards, roads, pastures were disinfected several times.

- On 17.01.2011 start of the clinical examination and blood sampling in the villagelocated in 10 km surveillance zones around Rezovo ( no villages in 3 km protective )

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Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages the blood samples taken from these animals are listed inAnnex II

19.01.2011By COMMISSION DECISION 2011/44/EU of 19 January 2011 concerning certain protectionmeasures against foot-and-mouth disease in Bulgaria, two more regions (Kardjali andHaskovo) were included in the zones with low risk (Annex II). Pursuant to the Decision nolive FMD susceptible animals and products thereof could live the territories of Annex I andno live FMD susceptible animals were allowed to leave the territories of Annex II.

Fig 3 Annex I and Annex II areas accroding to Decision 2011/44/EU

31.01.201121 days after the confirmed outbreak in Kosti (IP-1) and during the second round of clinicaland serological testing in the establiched 10 km surveillance zone around the outbreak (IP-1),new suspicion of FMD in Gramatikovo (Malko Tarnovo municipality, Burgas region)(Figure 4 (d) was detected on 30.01.2011.

NVS started the measures in relation with the suspicion according to the Directive 2003/85 on30.01.2011.

On 31.01.2011 the FMD NRL confirmed the disease:Cattle - 1 positive for NSP ELISA and FMDAb O type ELISASheep – 13 positive for NSP ELISA and Moab O type ELISA.Goats – 10 positive for NSP ELISA and Moab O type ELISA.

After the confirmation of FMD in the village on 31.01.2011 NVS started the implementationof the measures for control and eradication of FMD according to Council Directive2003/85/EC, namely:- On 31.01.2011 by Order of the Director General of NVS 3 km protective and 10 km

surveillance zones were defined around Gramatikovo village and the relevant restrictivemeasures were imposed.By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10km surveillance zones were removed.

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- On 31.01.2011 by Order of the Director General of NVS, NVS started killing of allsusceptible animals in the village of Gramatikovo. On 01.02.2011 all susceptibleanimals were killed.

- Cleaning and disinfection were carried out on the spot. Several disinfection was carriedout in the empty farms, animal holdings, backyards, roads, and pastures after killing ofthe animals.

- On 31.01.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around Gramatikovo.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages and the surveillance are listed in Annex III

Fig. 4. Location of the FMD outbreaks in Burgas region; a) WB wild boar shot; b) 1. Kosti; c) 2. Rezovo; d) 3.Gramatikovo

31.01.2011 – 28.02.2011Clinical examinations and blood sampling of susceptible animals in all villages in the riskmunicipalities of Burgas region. In the villages (nearby the Turkish border and in the villageswithin the 3 km protection zones and 10 km surveillance zones around the 3 outbreaks allsusceptible animals were blood sampled. Susceptible animals in all other villages outside the3 km protection zones and 10 km surveillance zones around the 3 outbreaks in Burgas regionwere sampled according to an estimated 5 % sero-prevalence and 95% confidence. (Please seeAnnex IV)

01.03.2011 – 18.03.2011

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Clinical examinations of susceptible animals in all the villages in the risk municipalities ofBurgas region, on the base of the requirements of Annex III of the Directive 2003/85/EC

18.03.2011On 18.03.2011 infected animals were detected in a bovine farm of 143 cattle, during theclinical surveillance (time period; 01.03.2011 to 18.03.2011) of susceptible animals in thevillages in the risk municipalities of Burgas region. The bovine farms is located at about 3 kmsouth of the village of Kirovo, Sredets municipality, Burgas region, 7 km north of the borderwith Turkey and 42 km from the last outbreak in Gramatikovo (IP-3) ( fig 5 and 5a). Thebovines were bred extensively in fenced forest area.

About 28 of 60 inspected that day bovines in the farm showed clinical signs of FMD(salivation and mouth lesions 4-5 days old ).

The owner of the affected farm observed changes in the health status in several cattle 2 daysago. He presumed that the reason of abnormal behaviour of animals is because of feedingwith non quality hay which was the reason for the delayed notification to the veterinaryservice.

Since January 2011 the aforementioned bovine herd and the animals in Kirovo village wereclinical examinated and blood sampled 5 rounds with negative results (07-14.01.2011; 28.01-03.02.2011; 07-10.02.2011, 16-20.02.2011 – NSP negative and 24-28.02.2011 negative forantibody to SP of the FMDV (Mono ELISA O-type Prionics)-see Annex IV).

Fig. 5. Location of the infected farm (IP-4 outbreak-Kirovo) and the distance from IP-3 –Gramatikovo)

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Fig. 5a. Location of the infected farm (IP-4 outbreak-Kirovo) and with 3 km protective and 10 km surveillancezones around the outbreak

NVS started the measures in relation with the suspicion according to the Directive 2003/85 on18.03.2011.

On 19th March the NRL confirmed the diagnosis (15 samples RT PCR positive)

BSFA started the measures for control and eradication of FMD pursuant to Directive2003/85/EC on 19.01.2011:- On 19.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 km

surveillance zones were defined around Kirovo village and the relevant restrictivemeasures were imposed.By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10km surveillance zones were removed.

- Killing of animals was ordered on 19.03.2011 by Order of the Executive Director ofBFSA. On 19.03.2011 BFSA started killing of all susceptible animals in the village ofKirovo. By reason of the type of animal husbandry in the area (free range) the time ofkilling took 3 days. The farms occupy an area of 400dka size and the personsresponsible for the killing of animals had some difficulties to perform theirs duties.


- On 19.03.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around Kirovo.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages the surveillance carried out are listed in Annex V

23.03.2011Infected bovine herd detected in Granichar village (Sredets municipality, Burgas region) (IP-5) (Figure 6) in the framework of routine clinical examinations carried out in the established

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10 km surveillance zones around Kirovo outbreak ( IP-4). The village is located at a distanceof 3km from the border with Turkey. In the village there was only one herd with 133 bovines.No other susceptible animal species were kept there.

15 bovines from the herd disiplayed clinical signs of FMD (salivation and about 4-5 days oldmouth lesions). The owner of the affected farm did not observed any changes in the healthstatus of the cattle. The animals were kept in free range area and the owner could observethese animals only in case of additional feeding.

Since January 2011 the bovine herd was clinical examinated and blood sampled in 5 roundswith negative results (08Jan, 28Jan, 07Feb, 16Feb - negative for NSP antibodies to FMD and25Feb - negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)-see AnnexIV. All clinical examination in the herd carried out during the period 28.02-18.03.2011 werenegative for FMD clinical signs.

On 23.03.2011 BFSA started the measures in relation with the suspicion according to theDirective 2003/85

On 24th March the NRL in Sofia confirmed the diagnosis: 3 positives under RT PCR and 7positives under FMDAb O type ELISA

Fig6. Location of the outbreaks Granitchar and Kirovo (infected farm) in Sredets municipality, Burgas regionwith the 3 km protection and 10 km surveillance zones around the two outbreaks

BSFA started the measures for control and eradication of FMD pursuant to Directive2003/85/EC on 24.03.2011:- On 24.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 km

surveillance zones were defined around Granitchar. village and the relevant restrictivemeasures were imposed.By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10km surveillance zones were removed.

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- On 24.03.2011 by Order of the Executive Director of BFSA, BFSA started killing of allsusceptible animals in the village of Granitchar. The killing of animals took 1day.


- On 24.03.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around Granichar.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages the blood samples taken from these animals are listed inAnnex VI

24.03.2011On 24th March three new suspicions detected in the municipality of Sredets by FMD clinicalsigns in the framework of clinical examinations in villages Goliamo Bukovo, Fakia, MominaTsurkva (fig.7). Suspisions in Goliamo Bukovo and Fakia were raised in the framework ofclinical examinations carried out in the established 10 km surveillance zone around Kirovooutbreak ( IP-4). The suspicion in Momina Tsurkva arised from additional and extendedclinical surveillance carried out in Sredets municipality.

On 24.03.2011 BFSA started the measures in relation with the suspicion according to theDirective 2003/85 in three villages in Sredets municipality. The herds in these 3 villages wereblood sampled 5 rounds with negative results (07-14.01.2011; 28.01-03.02.2011; 07-10.02.2011, 16-20.02.2011 - negative for NSP antibodies to FMD and 24-28.02.2011 -negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)-see Annex IV)since January.

The FMD diagnosis was confirmed by NRL /PCR and Ag ELISA positive, Ab negative) on25th March as follow:

IP-6 Outbreak in mixed cattle and pigs farm (49 cattle and 11 pigs) located nearbyGoliamo Bukovo village, the village is located in the 10km surveillance zone aroundIP-4 the outbreak in Kirovo: 4 positive cattle under RT-PCR, 3 of them FMD AgELISA positive, all 4 tested animals negative for antibody against SP of the FMDV(Mono - O type ELISA Prionics)

IP-7 Bovine farm (81 cattle) nearby the village of Fakia. One animal was confirmedpositive under RT-PCR and FMD Ag ELISA. The village is located in the 10kmsurveillance zone around the outbreak Kirovo (IP 4)

IP-8 Bovine farm (209 cattle) nearby Momina Tsarkva village. Two amiamls wereconfirmed positive under RT-PCR and FMD Ag ELISA. The village is located in the 10kmsurveillance zone around the outbreak Fakia (VII outbreak).

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Figure 7 Location of the three outbreaks ( IP-6, IP-7, IP-8) in Sredets municipality, Burgas region with the 3km protection and 10 km surveillance zones .

On 25.03.2011, BSFA started the measures for control and eradication of FMD pursuant toDirective 2003/85/EC:

- On 25.03.2011 by Order of the Executive Director of BFSA 3 km protective and 10 kmsurveillance zones were defined around the villages and the relevant restrictive measureswere imposed.The 3 km protective and 10 km surveillance zones were removed by Order of theExecutive Director of BFSA on 04.07.2011.

- The killing of susceptible animals in the infected farms was ordered on 25.03.2011 byOrder of the Executive Director of BFSA. The killing of animals took 1 day peroutbreak.


- On 25.03.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around the outbreaks.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages the blood samples taken from these animals are listed inAnnex VIII

26.03.2011Implementation of measures for control and eradication of FMD according to CouncilDirective 2003/85/EC; clinical and epidemiological examinations as well as blood sampling

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of susceptible animals in the risk villages located in Burgas and the neighboring to the westYambol region.

03.04.2011Two new outbreaks (fig 8) detected in the municipality of Malko Tarnovo in the frameworkof serological surveillance carried out in the established 10km surveillance zones aroundGranichar outbreak (IP-5):

IP-9 Bliznak village – located in the 10km surveillance zones around Granichar (IP-5) andthe farm near Goliamo Bukovo (IP-6). The animals kept in the village were clinicalexaminated and blood sampled 5 rounds with negative results (07-12.01.2011; 28.01-09.02.2011;15-20.02.11 negative for NSP antibodies to FMD; 25-28.02.2011;19.03-30.03.2011 negative for antibody to SP of the FMDV) since January.By phone conversation the NRL notified BFSA about Ab FMD positive results on03.04.2011, as follow:

Cattle - 21 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positiveSheep - 42 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positiveGoat’s - 36 samples FMD ELISA Type O Priocheck and FMD NSP ELISA positive

IP-10 Mixed buffalo,, sheep and goats farm 2 km south of Bliznak (Malko Tarnovomunicipality) in about 3 km north of the border with Turkey. On 03.04.2011 NRL informedby phone BFSA about Ab FMD positive results obtained in the framework of serologicalsurveillance, as follow:

Buffalos – 56 samples positive under ELISA Type O Priocheck , 43 positive forFMD NSP ELISASheep - 20 samples positive under ELISA Type O Priocheck and FMD NSP ELISAGoats - 36 positive under ELISA Type O Priocheck and FMD NSP ELISA.

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Fig 8. Location of the two outbreaks ( IP-9 and IP-10) in Malko Turnovo municipality, Burgas region with the 3km protection and 10 km surveillance zones .

On 03.04.2011 BFSA started the implementation of measures for control and eradication ofFMD in the outbreaks according to Directive 2003/85/EC

- 3 km protective and 10 km surveillance zones were defined around the both outbreaksand the relevant restrictive measures were imposed by Order of the Executive Directorof BFSA on 04.04.2011 (because the official letters from the FMD NRL concerning thepositive results are from 04.04.2011).By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10km surveillance zones were removed.

- The killing of susceptible animals in the infected farms was ordered on 04.04.2011 byOrder Executive Director of BFSA. The killing of animals took 2 days


- On 04.04.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around the outbreaks.

Map of the outbreaks, the villages in 3 km protective and 10 km surveillance zones, thenumber of animals present in the villages the blood samples taken from these animals arelisted in Annex IX

07.04.2011During the serological surveillance carried out in the established 3 km protective zonesaround Kirovo (IP-4) i Granichar outbreak (IP-5) new outbreak was detected on 7th of April in

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herd of sheep, goats and a bovine in Dolno Yabalkovo village (IP-11), Sredets municipality(fig 9).

Since January this herd has been clinical examinated and blood sampled for evidence of sero-conversion with negative results 6 times (07-14.01.2011; 28.01-03.02.2011; 07.02-10.02.2011, 16-20.02.2011 negative for NSP antibodies to FMD, 24-28.02.11, 19.03-30.03.2011 negative for antibody to SP of the FMDV – see Annex IV), but was now testedsero-positive when re-sampled on the 4th of April.

On 07.04.2011 the NRL notified BFSA for the Ab FMD positive samples, as follow:Cattle - 1 SP of the FMDV (Mono ELISA O-type Prionics) positive animal.Goats/sheep - 10 SP of the FMDV ( Mono ELISA O-type Prionics) positives

Fig 9. Location of the outbreaks (IP-10) in Sredets municipality, Burgas region with the 3 km protection and 10km surveillance zone

On 07.04.2011 BFSA started the implementation of measures for control and eradication ofFMD according to Directive 2003/85/EC:- 3 km protective and 10 km surveillance zones were defined around the outbreak and the

relevant restrictive measures were imposed by Order of the Executive Director of BFSAon 07.04.2011.By Order of the Executive Director of BFSA on 04.07.2011 the 3 km protective and 10km surveillance zones were removed.

- On 07.04.2011 according to the Order issued by the Executive Director of BFSA thekilling of the susceptible animals started. The killing of animals took 3 days.

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- On 07.04.2011 start of the clinical examination and blood sampling in the village in 3km protective and 10 km surveillance zones around the outbreak.

Map of the outbreak, the villages in 3 km protective and 10 km surveillance zones, the numberof animals present in the villages the blood samples taken from these animals are listed inAnnex X.

18.04.11-19.04.2011Clinical examinations and blood sampling of susceptible animals in the villages in the riskmunicipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna,Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulationin domestic livestock in the areas north and west of the detected outbreaks.(Please see AnnexXI)28.04.2011 – 29.04.2011Clinical examinations and blood sampling of susceptible animals in the villages in the riskmunicipalities of Burgas and Yambol regions. . (Please see Annex XII)

18.05.2011 – 19.05.2011Clinical examinations and blood sampling of susceptible animals in the villages in the riskmunicipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna,Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulationin domestic livestock in the areas north and west of the detected outbreaks. . (Please seeAnnex XIII)

01.06.2011 – 25.08.2011Clinical examinations in susceptible animals in the villages in the risk municipalities ofBurgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen, Sliven,Yambol and Haskovo regions.

Further measures taken:

1. Commission Iimplementing Ddecision 2011/493/EU of 5th August 2011 approves plansubmitted by Bulgaria to the Commission on 4th April 2011 for the eradication of foot-and mouth disease in wild animals in South-East Bulgaria. The plan has to beimplemented within one year period since submission to Commission including certainpassive and active surveillance activities in wild life and domestic livestock in an 20 kmarea along the border with Turkey in South-East Bulgaria defined as “Cordon Sanitaire”.(Please see Annex XIV)

2. Commission Decision 2011/388/EC amended Annex I and II of theCommission Decision 2011/44/EC and reduced regions under restrictions. Therestrictive measures laid down with the Decision 2011/44/EC were implemented in thereduced regions listed in Decision 2011/388/EC.The restrictions laid down inCommission Decision 2011/44/EC amended by Commission Decision 2011/388/ECwere applied in these areas until 30.09.2011.

3. On 04.07.2011 3 km protective and 10 km surveillance zones imposed according to theDirective 2003/85 were removed the for all outbreaks. It was considered the protective

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and surveillance zones to be removed later, because of the epidemiological situation ofBurgas region, namely:

- the fact that the primary source of the FMD outbreaks in Bulgaria remainsunclear and the virus could have entered Bulgaria through infected wild boar (orroe deer, der deer) from Turkey

- the fact that wild animals could be FMD infected- the type of animal husbandry in the area (extremely extensive, free range),

impossible stabling of the animals because of lack of stables in severalsettlements.

The BFSA took the decision to remove 3 km protective and 10 km surveillance zonesaround all outbreaks in July on the basis of:

- the negative results from the serological examinations and the clinicalexaminations in domestic animals carried out on 18-19.05.2011 and the end ofJune showed absence of FMDV in these animals;

- the serological examinations in wildlife carried out by the end of June showedabsence of FMDV and presence of Ab FMD in small number of wild animals (71 wild boar tested – 2 serological positive, 23 roe deer tested – 3 serologicalpositive, 2 red deer – no serological positive by the end of June).

c) Vaccines and vaccination.Emergency vaccination as a control measure for FMD has not been implemented onthe territory of Bulgaria the country in the context of the outbreak in 2011.

2. FMD surveillance. Surveillance strategy and measures applied in relation with allFMD outbreaks.

Since the confirmtation of the first case of FMD in wild boar in Burgas region the measuresfor the control and eradication of FMD have been implemented immediately:

2.1 culling of all infected and - where necessary - susceptible animals in the affected villages,

2.2 designation of the risk areas ( 3km protective and and 10km surveillance) and imposing ofrestrictions in these areas.

A ban of transport and moving animals of susceptible species and products thereof from therestricted areas for the purposes of national consumption and of intra-Community trade wereenforced.

According to the Decision 2011/44/EU live animals could only leave the territory of Annex Ifollowing a clinical examination direct to the predetermined slaughterhouse in the regionslisted in Annex II. They were marked accordingly and their movement recorded in the centraldatabase. The animals were handled separately and not come into contact with other animalsand products thereof. The meat from animals originating from these animals was markedaccording to Annex IV to Commission Decision 2011/44/EU and not sent to other MemberStates but only used for regional consumption.

Since the confirmation of the first FMD outbreak NVS (BFSA) has worked in collaborationwith the Police Services in order to ensure avoiding of non legal animal movement on theterritory of the regions from Annex I and II to the Decision 2011/44/EU.

19

2.3 clinical examinations and sampling of animals in all villages of the restricted areas.

Daily clinical examinations of the susceptible animals were performed in the villages of themunicipalities Tsarevo, Malko Tarnovo, Sredets, Primorsko and Sozopol.

In the villages (nearby the Turkish border and in the villages within the 3 km protection zonesand 10 km surveillance zones around the outbreaks all susceptible animals were bloodsampled while in all other villages samples were taken according to an estimated 5 % sero-prevalence and 95% confidence. ( please see the Annexes I-XV). Repeated clinicalinspections were carried out and sampling has taken place in the course of the epidemic.However, it has also been the case that holdings were inspected and blood sampled twice ofeven more times negative, before they were found clinically positive for an outbreak ofFMD.

During the period 09.01 - 28.02.2011 were carried out serological examinations in the villageslocated in 3 km protective and 10 km surveillance zones around the outbreaks from the firstoutbreak wave:

IP-1 - in four sampling rounds – per 14 and 7 days. (See Annex I)IP-2 - in four sampling rounds – per 14 and 7 days. (See Annex II)IP-3 - in three sampling rounds – per 7 days. (See Annex III)

The next sampling round in these villages was carried out during the time period 16.08-25.08.2011(see Annex VIII). (All laboratory results are serologically negative)

Serological examinations in the villages located in 3 km protective and 10 km surveillancezones around the outbreaks from the second outbreak wave (IP-4 to IP-11) were carried out inthe time period 09.01-25.08.2011 (5 sampling rounds before notification of the outbreak – in14 and 7 days, (see Annex IV) and 5 sampling rounds after the notification of the outbreak –per 21 and 9 days (see Annexes IX -XIII), as all laboratory results are serological negative.

2.4 Surveillance in East-Balkan pigs434 blood samples were taken from East-Balkan pigs from 11 herds reared in snoopilymunicipality which is located noth of the risk area. All tested animals were negative for FMD.The details are shown in annex IV.

2.5 Surveillance in wild animals

Since February till end of August 2011 hunting and trapping of wild susceptible animals wascarried out for surveillance purposes along the border with Turkey. During this period in total127 wild animals were sampled and tested both virologically and serologically. From 89 twowild boar over 1 year of age shot in the area of the first wave of outbreaks in domesticlivestock in January, two were serologically positive under Type O ELISA. At the same time36 roe deer and two red deer were shot and sampled of which 3 roe deer (2 shot near the areaof the second wave in March-April and one near the area of the first wave) were Ab positive.No FMDV has been detected.(See annex XIV)

2.6 epidemiological investigations (tracing foreword/tracing backwards)

2.7 public information campaigns targeting mainly animal holders and hunters

20

Special leaflet was distributed among hunters about FMD clinical signs in wild life and the4sampling procedure.

3.Epidemiological findings and considerations in relation with all FMD outbreaks

So far, the primary source of the FMD outbreaks in Bulgaria remains unclear; the virus couldhave entered Bulgaria through infected wild boar from Turkey. Alternatively, wild boar ordomestic pigs in Bulgaria could have had access to waste food or other means contaminatedwith FMDV.

The current extent of the infection in the wild boar and wild ruminant population and theduration of a sylvatic FMD epidemic are unknown. Should the entry mechanism be anundetected epidemic in wild boar in Turkish Thrace then introductions may have occurred atany other point along the border or spread silently in the susceptible wildlife population inthis region of Bulgaria as described above.

The length of the Bulgarian Turkish border is about 240 km; about half of it being a land andthe other half being a river border.

According to the results of the epidemiological investigations it is assumed that Kosti was thefirst village affected by FMD after the infected wild boar was shot and processed in thevillage by a farmer during the last two days of 2010 or 1st Jan 2011.

Concerning Rezovo the epidemiology remains unclear how and when the virus reached thisvillage. Full genome analysis indicates that the outbreak in Rezovo is not directly related tothe outbreak in Kosti, but may be traced, as the Kosti isolates and three isolate from the wildboar, to a common putative ancestor. There are frequent records of uncontrolled movement ofbuffalo from Turkey into Bulgaria, including those in October and November 2010.Uncontrolled twenty one buffaloes from Turkish territory came to the village in the beginningof January (4th January) and grazed together with the cattle from the village. The first clinicalsigns of FMD on the domestic animals originating from the Rezovo village were detected onthe 15th January. However, in a young buffalo ( yonger than 12 months age) FMD lesionswere observed on the date of killing of the buffalo herd (on 20.01.2011).The FMD NRLconfirmed positive for RT-PCR, NSP ELISA and O-type ELISA titre1:256 samples ( on25.01.2011) from this buffalo taken during the killing . The positive result for RT-PCR wasconfirmed by the EU Reference Laboratory for FMD in Pirbright on 23.02.2011.

During the killing samples were taken from more 7 buffaloes with positive results for NSPELISA and ELISA MONO-O-TYPE PRIONICS, confirmed by FMD NRL on25.01.2011.Data regarding the vaccination against FMD of eight of the 21 buffaloes wereobtained from the Turkish side; according to those data those 8 buffaloes were vaccinated inautumn 2010.

The outbreak in Gramatikovo most probably is a result of indirect contact through commongrazing ground used previously by infected cattle from Kosti. Only 1 cattle was kept inGramatikovo village which was grazed in common pasture with infected cattle herds fromvillige of Kosti. Several small ruminant herds were grazed in the same pasture as well.

So far, the source of the second wave of FMD outbreaks in Sredets municipality (Kirovo,Granichar, Goliamo Bukovo, Fakia, Momina Tsarkva, Dolno Yabalkovo) and Malko

21

Turnovo municipality(Bliznak), remains unclear; one of the hypotheses is that the virushave entered through infected wild boar. Full genome analysis indicates that the outbreaks inthat area are not directly related to the outbreak in Kosti or Rezovo, but may be traced, to acommon putative ancestor.

However, the full genome sequencing of isolates from the Kirovo outbreak conducted at theEU Reference Laboratory for FMD in Pirbright does not rule out the possibility that therewere four incursions of FMD in Bulgaria based on one common ancestor so far.

In conclusion and based on the current epidemiological results available, an EU expert group1

elaborated a timeline (Annex XV attached) leading to the working hypothesis that FMDoccurred in wildlife before infection of domestic animals in Kosti. Based on the clinical signsrecorded and the laboratory results (antibody and virus tests) it was assumed that the initialinfection happened between the 13th and 27th December 2010, although the 1st date couldeven be earlier than the 13th of December.

The tracebility of the animal movement in Kosti (IP-1) and Rezovo (IP-2) villages, Tsarevomunicipality showed that no animals left villages in the time period Nov 2010-09th of Jan2011. Bovines were kept in free range system without milking, small ruminants werepregnant and in dry milk period in these villages.The animals in the villages in Burgas regionwere reared and slaughtered for own consumption, so there is no movement of milk or meatin the villages. Furthermore there are no slaughterhouses in Tsarevo municipality whichprecludes the movement of animals for slaughtering.

There are two slaughterhouses in Burgas region and the made inspections showed that therewere no slaughtered animals from Kosti (IP-1), Rezovo (IP-2) and Gramatikovo (IP-3) inthese slaughterhouses.

4.Next steps

Clinical examinations and serosurveillance carried out from the last FMD outbreak ( on07.04.2011) to August 2011 showed lack of FMDV and FMD Ab in domestic animals, lack ofFMDV in the wildlife and presence of FMD Ab in small percentage of wildlife.

Commission Iimplementing Ddecision 2011/493/EU of 5th August 2011 approves the plansubmitted by Bulgaria to the Commission on 4th April 2011 for the eradication of foot- and-mouth disease in wild animals in South-East Bulgaria. The plan has to be implemented withinone year period since submission to Commission including certain passive and activesurveillance activities in wild life and domestic livestock in an area in South-East Bulgariadefined as “Cordon Sanitaire” area in South-East Bulgaria

“Cordon Sanitaire” is formed by a defined infected and a neighboring to north and west riskarea based on the results of the epidemiological considerations, the geographical distributionof the disease and the unknown FMD situation in the wildlife in Turkish Thrace.

The defined infected area (Area “A”) is a 20 km-wide region in south-east of Bulgaria alongthe Turkish border with a surface of about 1240 km2. In this area the outbreaks in domestic

1 Between 17 and 21 January 2011 an expert group of the Commission visited Bulgaria (Burgas region) toevaluate the FMD control and eradication measures on the spot. (LINK SCOFCAH)

22

livestock occurred in January and March-April 2011. The area comprises: part of Tsarevo,Malko Tarnovo and south part of Sredets municipalities (Region of Burgas). Within thedefined infected area 32 villages are located. The risk area with a surface of about 2160 km2 isdivided in two parts (Areas “B” and “C”) and is neighbouring the infected area in the northand west along the Turkish border. These areas have been defined taking into considerationthe unclear epidemiological situation of FMD more generally.

The risk area comprises the following municipalities or parts of them: North Tsarevo,Primorsko, Sozopol and North Sredets (Region of Burgas) which form area B and Svilengrad,Topolovgrad (Region of Haskovo), Elhovo, Boliarovo (Region of Yambol) which form areaC. Within the defined risk area 74 villages are located.(See Annex XIII )

Control in wildlife

The estimated wild boar population in the defined infected and risk areas together is about4500 animals. It is assumed that about 1500 wild boar are living in each area (A, B, C). Inrelation to wild ruminants (Capreolus capreolus and Cervus elaphus spp) the estimatednumber in the “Cordon Sanitaire” is about 5400. The existing data on population density andsize is based on spring counting system.

Serological and virological monitoring of wild life is performed in the defined areas. Thesampling strategy in wild boar has been chosen in analogy to the recommendations given forCSF wild boar monitoring, being the only practical guide so far for such purposes. Pursuant tothe plan the minimum number of animals to be sampled within a defined sampling area (A, Band C) has to be at least 59 animals to allow detection of 5% sero-prevalence with 95 %confidence. In addition to have a view on the situation in other susceptible wild animalssamples for virological and serological testing have to be taken from at least 35 heads of roedeer (Capreolus capreolus), red deer (Cervus elaphus) and other susceptible wild ruminantswithin a defined sampling area, to allow detection of 10% sero-prevalence with 95 %confidence.

Passive surveillance system is strengthened with the view of an early detecting the viralinfection. There is no farmed game on the territory of the defined area “Cordon Sanitare”.Hunters and gamekeepers are instructed to report findings of all dead wild boar or othersusceptible wild game (red deer, roe deer) to the veterinary service. Any carcass that is foundhas to be sampled and laboratory tested.

The animals to be sampled are either be hunted or trapped in wild boar traps (seven large wildboar traps installed along the border with Turkey in the municipalities of Tsarevo and MalkoTarnovo. The location of the traps is shown in Annex XIV).

Control in domestic livestock

Pursuant to the Plan for the eradication of foot and mouth disease in wild animals all 106villages in the Cordon Sanitaire are visited every 21 days by a team of veterinary experts andall susceptible animals are clinically examined in accordance with Annex III 1 of Directive2003/85/EC. In case of suspicion samples for laboratory testing (blood and tissue samples) aretaken immediately and measures laid down in Directive 2003/85/EC, Articles 4 and 5 applied.

23

In addition to clinical surveillance, routine blood sampling for serological tests is conductedevery third month. The minimum number of animals to be sampled in an epidemiological unitwill allow detection of 5% sero-prevalence with 95 % confidence. The epidemiological unit isthe village. In case of a pure sheep/goat population in the epidemiological unit the bloodsampling is carried within the rhythm of the clinical surveillance.

Blood sampling of animals for slaughter is also carried out before the leave the CordonSanitaire if they animals are not tested within 21 days period before with a negative result. Atthe slaughter house a thorough ante and post mortem inspection is be performed for detectingFMD at this stage.

A ban of transport and moving animals of susceptible species and products thereof from the“Cordon Sanitaire” for the purposes of national consumption and of intra-Community tradewill be enforced.

Ø Live animals will only leave the “Cordon Sanitaire” following a clinical examinationdirect to the predetermined slaughterhouse in the region of Burgas, Shuman, Sliven,Yambol and Haskovo (regional consumption). They will be marked accordingly andtheir movement recorded in the central database. There they will be handled separatelyand will not come into contact with other animals and products thereof. The meat fromanimals originating from the Cordon Sanitaire will be marked according to Annex IVto Commission Decision 2011/44/EU and will not be sent to other Member States butonly used for regional consumption.

Ø Meats of the bovine, ovine, caprine and porcine species and other bi-ungulatesproduced from animals of those species in the Cordon Sanitaire will be marked inaccordance with the health mark laid down in Annex IV to Decision 2011/44/EUbefore such meats are used for the regional consumption. No meat products will leavethe Cordon Sanitaire unless in compliance with Directive 2002/99/EC.

Ø Milk collected inside the Cordon Sanitaire and dairy products derived from such milkwill be treated as laid down in Articles 4 and 5 respectively of Decision 2011/44/EUbefore they leave this area for regional consumption only. Particular attention at theroad posts at the exists of the Cordon Sanitaire will be paid to milk collectors’ means oftransport to avoid spill over of FMD from infected to unaffected areas.

Ø Other products of animal origin collected from FMD susceptible domestic species willnot be permitted to leave the “Cordon Sanitaire”. In particular, animal by-products willbe collected through the Bulgarian rendering system and disposed at the renderingplants at Varna and Shumen or be buried on the spot after treatment with Virkon S orequivalent under the supervision of the representatives of the BGFSA. Hides and skinsshall be treated as laid down in Article 8 of Decision 2011/44/EU .

*Please see below the relevant Annexes

24

25

Annex I

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUNDTHE OUTBREAK IN THE VILLAGE OF KOSTI, TSAREVO MUNICIPALITY

(Disease confirmed on 9th Jan) (Outbreak-IP-1)

Fig 10. Location of the settlements in the 10 km surveillance zone around Kosti village, Tsarevo municipality

There are no villages located in the 3 km protection zone. There are 5 villages located in the 10 km surveillance zone of which 4 (Brodilovo, Izgrev,Balgari and Kondolovo) belong to Tsarevo municipality and 1 (Gramatikovo) belongs to Malko Tarnovo municipality.

26

Table 1. First blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village Kosti,Tsarevo Municipality (end of sampling 11.01.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken present Samples takenSettlements

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

1. Kostioutbreak 4 196 4 132 21 121 18 117 44 205 29 149 8 136 3 21

Settlements in the 10km surveillance zone (Tsarevo Municipality)4. Brodilovo 3 8 3 5 26 117 26 93 47 164 6 83 3 44 1 185. Balgari 0 0 0 0 5 39 5 40 6 12 6 12 0 0 0 06. Kondolovo 0 0 0 0 3 48 3 47 1 39 1 37 0 0 0 07. Izgrev 0 0 0 0 1 4 0 0 0 0 0 0 1 13 0 0

Settlements in the 10km surveillance zone (Malko Tarnovo municipality)3. Gramatikovo 1 1 1 1 5 192 2 64 24 111 2 71 0 0 0 0Total 8 205 8 138 61 521 54 361 122 531 44 352 12 193 4 39Results:Kosti village:Cattle- 1 positive for NSP ELISA and antibodies FMD type O ELISASheep – 14 positive for NSP ELISA and antibodies FMD type O ELISAGoats – 12 positive for NSP ELISA tests and antibodies FMD type O ELISA.Pigs – 8 positive for NSP ELISA.

All other samples are NSP negative .

Table 2. Second blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village ofKosti, Tsarevo Municipality (28.01-02.02.2011)

Large ruminants sheep goats pigsSettlementspresent Samples taken present Samples taken present Samples taken present Samples taken

27

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

1. Kostioutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 10km surveillance zone in Tsarevo Municipality4. Brodilovo 3 8 3 8 26 117 26 117 47 164 47 164 3 44 3 445. Balgari 0 0 0 0 5 39 5 39 6 12 6 12 0 0 0 06. Kondolovo 0 0 0 0 3 48 3 48 1 39 1 39 0 0 0 07. Izgrev 0 0 0 0 1 4 1 4 0 0 0 0 1 13 1 13

Settlements in the 10km surveillance zone in Malko Tarnovo municipality3. Gramatikovo 1 1 1 1 5 192 5 192 24 111 24 111 0 0 0 0Total 4 9 4 9 40 400 40 400 78 326 78 326 4 57 4 57Note:* Kosti – no samples taken as all susceptible animals in the village were killed and buried by 11.01.11., because detection of FMD outbreak in the village.**The FMD detected in 1 bovine and 13 small ruminant in Gramatikovo village, Malko Tarnovo municipality on 31.01.2011.. Killing and burial of all susceptible animals in the villagewas carried out on 01.02.2011All other samples are NSP negative

Table 3. Third blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Kosti,Tsarevo Municipality (08-09.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

1. Kostioutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Settlements in the 10km surveillance zone in Malko Tarnovo municipality3. Gramatikovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

28

Total 3 8 0 0 35 208 4 44 54 215 4 39 4 57 2 32* Kosti and Gramatikovo – no samples taken as all susceptible animals in the village were killed and buried by 11.01.11 ( Kosti village) and on 01.02.2011 ( Gramatikovo village),because detection of FMD outbreak in the village.All other samples are NSP negative

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village of Kosti,Tsarevo Municipality (23.02-28.02.2011)

Large ruminants sheep goats pigsSamples taken present Samples taken present Samples taken present Samples taken presentSettlements№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Kostioutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Settlements in the 10km surveillance zone in Malko Turnovo Municipality3.Gramatikovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 3 8 3 8 10 200 10 200 54 215 54 215 4 57 4 57All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

29

Annex IISURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND

THE OUTBREAK IN THE VILLAGE OF REZOVO, TSAREVO MUNICIPALITY(Disease detected clinically on 15th Jan) (Outbreak IP-2)

Fig 11. Location of the settlements in the 10 km surveillance zone around Rezovo village, Tsarevo municipality

In the 3km protection zone there are no settlements. In the 10 km surveillance zone is located only one village – Sinemorets.

30

Table 1. Blood sampling (before the detection of the outbreak) of FMD susceptible animals in the villages of Rezovo and Sinemorets, TsarevoMunicipality (8-11.01.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

2. Rezovo 6 87 6 26 2 68 2 33 2 8 1 3 1 29 0 08. Sinemorets 3 14 3 13 9 220 5 120 10 79 3 63 6 9 6 9Total: 9 101 9 39 11 288 7 153 12 87 4 66 7 38 6 9

The laboratory results:Rezovo:Cattle - 19 samples positive for RT-PCR, 12 positive for Ag ELISA FMDPigs - 3 positive Ag ELISA FMDAll other samples (in Rezovo and Sinemorets) are negative for NSP antibodies to FMD.

Table 2. First blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village ofRezovo, Tsarevo Municipality (28.01-02.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

2. Rezovooutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 10km surveillance zone in Tsarevo Municipality8. Sinemorets 3 14 3 14 9 220 9 220 10 79 3 63 6 9 3 9Total: 3 14 3 14 9 220 9 220 10 79 3 63 6 9 3 9

All samples from Sinemorets: NSP negative .

31

Table 3. Second blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village ofRezovo, Tsarevo Municipality (08-09.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

2. Rezovooutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 10km surveillance zone in Tsarevo Municipality8. Sinemorets 3 14 2 8 9 220 2 71 10 79 1 13 6 9 1 2Total 3 14 2 8 9 220 2 71 20 79 1 13 6 9 1 2

All samples from Sinemorets: NSP negative.

Table 4. Fourth blood sampling of FMD susceptible animals in the settlements in the 10 km surveillance zone around the outbreak in the village ofRezovo, Tsarevo Municipality ( 23.02.2011-28.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

2. Rezovooutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 10km surveillance zone in Tsarevo Municipality8. Sinemorets 3 15 3 15 9 215 9 215 10 79 10 79 6 9 6 9Total 3 15 3 15 9 215 9 215 10 79 10 79 6 9 6 9

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

32

Annex III

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUNDTHE OUTBREAK IN THE VILLAGE OF GRAMATIKOVO, MALKO TARNOVO MUNICIPALITY

(Disease confirmed on 31st Jan) ( Outbreak IP-3)

Fig 12. Location of the settlements in the 10 km surveillance zone around Gramatikovo, Malko Tarnovo municipality

In the 3 km protection zone is located only one village (Kondolovo, Tsarevo municipality). There are 5 villages located in the 10 km surveillance zone ofwhich 2 (Balgari and Kosti) belong to Tsarevo municipality and 3 (Slivarovo, Zabrenovo and Vizitsa) belong to Malko Tarnovo municipality.

33

Table. 1. First blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak inGramatikovo, Malko Tarnovo Municipality. (end of sampling 08.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

3. Gramatikovooutbreak* 1 1 1 1 5 192 5 192 24 111 24 111 0 0 0 0

Settlements in the 3 km protection zone4. Kondolovo 0 0 0 0 3 48 3 48 1 39 1 39 0 0 0 0

Settlements in the 10km surveillance zone1. Slivarovo 0 0 0 0 3 38 3 38 3 48 3 36 3 12 3 122. Zabernovo 3 22 2 6 3 9 3 9 12 112 11 73 8 61 3 403. Vizitsa 4 57 4 24 5 84 5 55 2 21 2 21 0 0 0 05. Balgari 0 0 0 0 5 39 5 39 6 12 6 12 0 0 0 06. Kosti** 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 8 80 7 31 24 410 24 381 48 343 47 292 11 73 6 52

Note:*Gramatikovo – samples taken during detection of the outbreak on 30.01.2011 and during killing of the animals on 01.02.2011** Kosti - all susceptible animals in the village were killed and buried , because detection of FMD outbreak in the village.

The laboratory resultsGramatikovo:cattle - 1 samples positive for NS ELISA and FMDAb O type ELISAsheep – 13 positive for NS ELISA and FMDAb O type ELISA.goats – 10 positive for NS ELISA and FMDAb O type ELISA

All samples from the other villages were NSP negative .

Table. 2. Second blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreakin Gramatikovo, Malko Tarnovo Municipality (13-16.02.2011)

Settlements Large ruminants sheep goats pigs

34

present Samples taken present Samples taken present Samples taken present Samples taken№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals3. Gramatikovooutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Settlements in the 10km surveillance zone1. Slivarovo 0 0 0 0 3 38 3 38 3 48 3 36 3 12 1 72. Zabernovo 3 22 3 19 3 9 0 0 12 112 5 67 8 61 2 253. Vizitsa 4 57 4 33 5 84 2 81 2 21 1 4 0 0 0 05. Balgari 0 0 0 0 5 39 1 10 6 12 1 2 0 0 0 06. Kosti 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 7 79 7 52 29 218 7 136 24 232 11 137 11 73 3 32

All samples NSP negative.

Table. 3.Third blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreakin Gramatikovo, Malko Tarnovo Municipality (23.02.11-28.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

3. Gramatikovooutbreak 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Settlements in the 10km surveillance zone1. Slivarovo 0 0 0 0 3 38 3 38 3 36 3 36 1 7 1 72. Zabernovo 3 22 3 22 3 9 1 4 12 112 12 112 8 61 4 333. Vizitsa 4 57 4 10 5 84 4 80 2 21 2 20 0 0 0 05. Balgari 0 0 0 0 5 39 5 36 6 12 6 11 0 0 0 06. Kosti 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 7 77 7 32 29 213 16 201 24 220 24 218 9 68 5 40

All samples negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

35

Annex IVSURVEILLANCE IN THE SETTLEMENTS OF BURGAS REGION WHICH ARE LOCATED

OUTSIDE THE PROTECTION AND SURVEILLANCE ZONES AROUND THE OUTBREAKS IN JANUARY 2011

(All animals in the villages outside the protection and surveillance zones that are not sampled are clinically checked)

I. SURVEILLANCE IN TSAREVO MUNICIPALITY

Fig13. Location of the settlements in Tsarevo municipality

36

Table 1. First blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside of protection and surveillance zones (07-11.01.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken present Samples takensettlement

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№ herds

№animals

№herds

№ animals

№ herds

№animals

№ herds

№animals

3. Ahtopol 11 107 3 53 18 580 3 253 14 116 8 23 3 123 1 28. Varvara 4 26 6 27 11 166 8 118 11 52 3 36 0 0 0 012. Velika 2 57 3 44 2 28 2 21 4 57 3 35 0 0 0 011. Lozenets 0 0 2 22 11 302 8 175 6 181 5 112 1 14 2 62. Sinemorets 3 14 4 13 9 220 5 120 10 79 3 63 6 9 6 913. Fazanovo 4 174 4 68 1 290 1 54 0 0 0 0 1 25 0 010. Tsarevo 8 39 8 25 18 763 15 186 13 31 4 78 2 17 0 01. Rezovo 6 87 6 26 2 68 2 33 2 8 1 3 1 29 0 0Total 38 504 36 278 72 2417 44 960 60 524 27 350 14 217 9 17


Table 2. Second blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside of protection and surveillance zones(28.01-02.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

3. Ahtopol 11 107 11 107 18 580 18 580 14 116 14 116 3 123 3 398. Varvara 4 26 4 26 11 166 11 166 11 52 11 52 0 0 0 012. Velika 2 57 2 32 2 28 2 21 4 57 1 24 0 0 0 011. Lozenets 0 0 0 0 11 302 11 87 6 181 5 56 1 14 1 1413. Fazanovo 4 174 4 101 1 290 1 56 0 0 0 0 1 25 1 1310. Tsarevo 8 39 8 39 18 763 18 246 13 31 13 31 2 17 0 01. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 29 403 29 305 61 2129 61 1156 48 437 44 279 7 179 5 66

Note: FMD outbreak confirmed in Rezovo village on 15.01.2011 and all susceptible animals in the village were killed and buried by 20.01.2011.All samples NSP negative.

37

Table 3. Third blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones(08-09.02.2011)


№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

3. Ahtopol 11 107 0 0 18 580 1 50 14 116 3 123 2 308. Varvara 4 26 0 0 11 166 3 77 11 52 2 15 0 0 0 012. Velika 2 57 1 31 2 28 1 16 4 57 1 16 0 0 0 011. Lozenets 0 0 1 7 11 302 1 46 6 181 0 0 1 14 1 1413. Fazanovo 4 174 1 49 1 290 1 54 0 0 0 0 1 25 1 2010. Tsarevo 8 39 1 8 18 763 3 124 13 31 3 21 2 17 0 01. Rezovo* 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 29 403 4 95 61 2129 10 367 48 437 6 52 7 179 4 64


Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones(15-20.02.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present present Samples takensettlement

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds



38

Table 5. Fifth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones(26-28.02.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken presentsettlement

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds



39

II. SURVEILLANCE IN MALKO TARNOVO MUNICIPALITY

Fig 14. Location of the settlements in Malko Tarnovo municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Malko Tarnovo municipality outside of protection and surveillance zones(07-12.01.2011)


№herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

40

2. MalkoTarnovo 10 102 7 71 37 681 7 309 47 312 4 99 12 118 3 10013. Bliznak 6 79 3 49 10 82 4 75 7 52 2 35 1 2 1 25. Brashlian 3 4 3 4 0 0 0 0 0 0 0 0 0 0 0 010. Biala Voda 1 9 1 8 4 32 4 23 2 38 2 43 2 29 1 137. Vizitsa 4 57 2 30 5 84 4 83 2 21 2 21 0 0 0 012. Evrinozovo 1 10 1 6 1 18 1 14 2 21 2 21 1 27 0 06. Zabernovo 3 22 3 24 3 9 1 4 12 112 7 108 8 61 8 589. Zvezdets 7 218 3 117 8 116 1 38 6 73 4 62 5 66 1 158. Kalovo 0 0 0 0 3 59 2 36 2 51 2 51 3 33 1 811. Mladejko 0 0 0 0 0 0 0 0 9 41 9 41 0 0 0 01. Slivarovo 0 0 0 0 3 38 3 32 3 48 3 48 3 12 1 94. Stoilovo 3 4 3 4 5 76 2 13 14 65 13 48 0 0 0 0Total 38 505 26 313 79 1195 29 627 106 834 50 577 35 348 16 205


Table 2. Second blood sampling of FMD susceptible animals in the settlements of Malko Tarnovo municipality outside of protection and surveillancezones (28.01-09.02.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken Samples taken presentsettlement

№herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

2. MalkoTarnovo 10 102 10 81 37 681 23 324 47 312 23 154 12 118 7 10513. Bliznak 6 79 6 32 10 82 10 59 7 52 7 28 1 2 1 25. Brashlian 3 4 2 2 0 0 0 0 0 0 0 0 0 0 0 010. Biala Voda 1 9 1 9 4 32 2 20 2 38 2 32 2 29 2 2912. Evrinozovo 1 10 1 10 1 18 1 14 2 21 1 10 1 27 1 199. Zvezdets 7 218 7 119 8 116 2 30 6 73 1 40 5 66 3 438. Kalovo 0 0 0 0 3 59 2 59 2 51 1 5 3 33 1 611. Mladejko 0 0 0 0 0 0 0 0 9 41 9 41 0 0 0 04. Stoilovo 3 4 0 0 5 76 5 61 14 65 6 23 0 0 0 0Total 31 426 27 253 68 1064 45 567 89 653 50 333 24 275 15 203


41

Table 3. Third blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones(15-20.02.2011)


№herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№herds

№animals

№ herds

№animals

№ herds

№animals

2. MalkoTarnovo 10 102 1 19 37 681 2 55 47 312 2 14 12 118 1 1213. Bliznak 6 79 0 0 10 82 0 0 7 52 0 0 1 2 0 05. Brashlian 3 4 0 0 0 0 0 0 0 0 0 0 0 0 0 010. Biala Voda 1 9 0 0 4 32 0 0 2 38 0 0 2 29 0 012. Evrinozovo 1 10 0 0 1 18 0 0 2 21 0 0 1 27 0 09. Zvezdets 7 218 0 0 8 116 1 20 6 73 1 20 5 66 1 158. Kalovo 0 0 0 0 3 59 1 17 2 51 1 18 3 33 1 1711. Mladejko 0 0 0 0 0 0 0 0 9 41 0 0 0 0 0 04. Stoilovo 3 4 0 0 5 76 2 32 14 65 1 6 0 0 0 0Total 31 426 1 19 68 1064 6 131 89 653 5 52 24 275 3 44


Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Tsarevo municipality outside protection and surveillance zones(26-28.02.2011)

settlement Large ruminants sheep goatspresent Samples taken present Samples taken present Samples taken present Samples takensettlement

№herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

№ herds

№animals

2. MalkoTarnovo 10 102 1 7 37 681 9 90 47 312 17 93 12 118 0 013. Bliznak 6 79 0 0 10 82 0 0 7 52 0 0 1 2 0 05. Brashlian 3 4 0 0 0 0 0 0 0 0 0 0 0 0 0 010. Biala Voda 1 9 1 9 4 32 2 29 2 38 1 20 2 29 1 1312. Evrinozovo 1 10 0 0 1 18 0 0 2 21 0 0 1 27 0 09. Zvezdets 7 218 2 48 8 116 0 0 6 73 0 0 5 66 0 08. Kalovo 0 0 0 0 3 59 2 27 2 51 3 26 3 33 2 10

42

11. Mladejko 0 0 0 0 0 0 0 0 9 41 9 32 0 0 0 04. Stoilovo 3 4 0 0 5 76 4 52 14 65 9 46 0 0 0 0Total 31 426 68 1064 89 653 24 275


III. SURVEILLANCE IN SREDETS MUNICIPALITY

Fig 15. Location of the settlements under surveillance in Sredets municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Sredets municipality (07-14.01.2011)Large ruminants sheep goats pigssettlement

present Samples taken present Samples taken present Samples taken present Samples taken

43

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

1. Belevren 6 136 4 48 0 0 0 0 3 7 3 7 0 0 0 02. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 03. Varovnik 2 204 2 92 0 0 0 0 0 0 0 0 0 0 0 04. Valchanovo 2 89 2 33 7 341 7 142 3 158 3 64 0 0 0 05. GoliamoBukovo 7 476 5 90 2 100 2 47 13 57 13 57 1 3 0 06. GornoYabalkovo 2 80 2 54 6 348 3 216 6 56 3 20 0 0 0 07. Granichar 2 229 1 50 0 0 0 0 0 0 0 0 0 0 0 08. DolnoYabalkovo 1 37 1 31 1 26 1 17 3 157 3 115 0 0 0 09. Kamene 1 194 1 27 4 313 4 68 1 86 1 16 0 0 0 010. Kirovo 2 183 1 37 1 7 1 7 3 30 2 17 0 0 0 011. MominaTsarkva 3 266 3 82 31 2324 31 366 30 142 30 133 0 0 0 012. Panchevo 0 0 0 0 2 580 2 56 4 70 4 30 1 14 1 613. Slivovo 1 34 1 22 2 154 2 68 8 70 8 67 0 0 0 014. Trakiitsi 0 0 0 0 1 9 1 7 2 178 1 47 2 93 1 2015. Fakia 5 288 4 121 14 421 10 202 40 252 42 239 0 0 0 0Total 34 2216 27 687 71 4623 64 1196 116 1263 113 812 4 110 2 26


Table 2. Second blood sampling of FMD susceptible animals in the settlements of Sredets municipality (28.01-03.02.2011)Large ruminants sheep goats pigs

present Samples taken present Samples taken present Samples taken present Samples takensettlement№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Belevren 6 136 6 125 0 0 0 0 3 7 3 7 0 0 0 02. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 03. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 04. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 0

44

5. Goliamo Bukovo 7 476 4 188 2 100 2 55 13 57 12 52 1 3 0 06. GornoYabalkovo 2 80 2 73 6 348 6 348 6 56 6 56 0 0 0 07. Granichar 2 229 2 154 0 0 0 0 0 0 0 0 0 0 0 08. DolnoYabalkovo 1 37 1 37 1 26 1 22 3 157 3 108 0 0 0 09. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 010. Kirovo 2 183 2 95 1 7 1 7 3 30 2 17 0 0 0 011. MominaTsarkva 3 266 3 95 31 2324 31 387 30 142 30 139 0 0 0 012. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 013. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 0 0 014. Trakiitsi 0 0 0 0 1 9 1 9 2 178 1 47 2 93 1 2315. Fakia 5 288 0 168 14 421 0 212 40 252 0 243 0 0 0 0Total 34 2216 20 767 71 4623 42 828 116 1263 57 426 4 110 1 23


Table 3. Third blood sampling of FMD susceptible animals in the settlements of Sredets municipality (07-10.02.2011).Large ruminants sheep goats pigs


herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Belevren 6 136 2 54 0 0 0 0 3 7 0 0 0 0 0 02. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 03. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 04. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 05. Goliamo Bukovo 7 476 3 121 2 100 0 0 13 57 0 0 1 3 1 36. Gorno Yabalkovo 2 80 1 38 6 348 2 93 6 56 1 3 0 0 0 07. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 08. Dolno Yabalkovo 1 37 1 31 1 26 1 25 3 157 1 20 0 0 0 09. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 010. Kirovo 2 183 1 47 1 7 0 0 3 30 1 13 0 0 0 011. Momina Tsarkva 3 266 1 45 31 2324 1 57 30 142 0 0 0 0 0 012. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 0

45

13. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 014. Trakiitsi 0 0 0 0 1 9 0 0 2 178 1 43 2 93 1 2415. Fakia 5 288 0 0 14 421 1 53 40 252 0 0 0 0 0 0Total 34 2216 10 384 71 4623 5 228 116 1263 4 79 4 110 2 27


Table 4. Fourth blood sampling of FMD susceptible animals in the settlements of Sredets municipality (16-20.02.2011).Large ruminants sheep goats pigs


herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Belevren 6 136 6 70 0 0 0 0 3 7 3 7 0 0 0 02. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 03. Varovnik 2 204 0 0 0 0 0 0 0 0 0 0 0 0 0 04. Valchanovo 2 89 2 33 7 341 7 142 3 158 3 64 0 0 0 05. Goliamo Bukovo 7 476 3 121 2 100 0 0 13 57 0 0 1 3 1 36. Gorno Yabalkovo 2 80 1 38 6 348 0 90 6 56 0 0 0 0 0 07. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 08. Dolno Yabalkovo 1 37 1 31 1 26 1 24 3 157 1 19 0 0 0 09. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 010. Kirovo 2 183 1 48 1 7 0 0 3 30 1 13 0 0 0 011. Momina Tsarkva 3 266 0 51 31 2324 0 57 30 142 0 0 0 0 0 012. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 013. Slivovo 1 34 1 22 2 154 2 68 8 70 65 0 0 014. Trakiitsi 0 0 0 0 1 9 0 0 2 178 1 43 2 93 1 2415. Fakia 5 288 0 0 14 421 1 53 40 252 0 0 0 0 0 0Total 34 2216 16 462 71 4623 11 366 116 1263 68 139 4 110 2 27


Table 5. Fifth blood sampling of FMD susceptible animals in the settlements of Sredets municipality (25-28.02.2011).

Large ruminants sheep goats pigssettlementpresent Samples taken present Samples taken present Samples taken present Samples taken

46

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

1. Belevren 6 136 2 71 0 0 0 0 3 7 1 7 0 0 0 02. Bogdanovo 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 03. Varovnik 2 204 0 90 0 0 0 0 0 0 0 0 0 0 0 04. Valchanovo 2 89 0 0 7 341 0 0 3 158 0 0 0 0 0 05. Goliamo Bukovo 7 476 3 142 2 100 2 55 13 57 13 47 1 3 1 36. Gorno Yabalkovo 2 80 1 38 6 348 2 93 6 56 1 3 0 0 0 07. Granichar 2 229 1 48 0 0 0 0 0 0 0 0 0 0 0 08. Dolno Yabalkovo 1 37 1 31 1 26 1 25 3 157 1 111 0 0 0 09. Kamene 1 194 0 0 4 313 0 0 1 86 0 0 0 0 0 010. Kirovo 2 183 1 48 1 7 0 7 3 30 1 17 0 0 0 011. Momina Tsarkva 3 266 0 90 31 2324 0 383 30 142 0 124 0 0 0 012. Panchevo 0 0 0 0 2 580 0 0 4 70 0 0 1 14 0 013. Slivovo 1 34 0 0 2 154 0 0 8 70 0 0 0 014. Trakiitsi 0 0 0 0 1 9 0 8 2 178 1 29 2 93 1 2415. Fakia 5 288 5 164 14 421 1 216 40 252 40 237 0 0 0 0Total 34 2216 9 722 71 4623 6 787 116 1263 45 451 4 110 2 27


47

IV. SURVEILLANCE IN PRIMORSKO MUNICIPALITY

Fig 16. Location of the settlements under surveillance in Primorsko municipality

Table 1. First blood sampling of FMD susceptible animals in the settlements of Primorsko municipality (12-14.01.2011)Large ruminants sheep goats pigs

present Samples taken present Samples taken present Samples taken present Samples takensettlement

№herds

№animals

№herds

№animals

№herds

№animal

s

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

1. Kiten 6 121 3 21 19 298 11 45 17 93 10 21 2 6 0 02. Pismenovo 1 14 2 6 5 206 5 91 4 17 2 13 2 13 2 93. Primorsko 11 112 0 0 12 472 11 63 17 96 8 9 8 128 0 04. Yasna Poliana 4 57 0 0 21 2074 5 124 29 187 0 0 19 160 0 05. Veselie 8 174 0 0 36 4702 0 0 12 78 0 0 9 43 0 0

48

6. Novo Panicharevo 6 97 0 0 22 2647 0 0 43 242 0 0 22 129 0 0Total 36 575 5 27 115 10399 32 323 122 713 20 43 62 479 2 9


Table 2. Second blood sampling of FMD susceptible animals in the settlements of Primorsko municipality (03.02-05.02.2011)Large ruminants sheep goats pigs


herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Kiten 6 121 5 66 19 298 19 104 17 93 14 42 2 6 2 42. Pismenovo 1 14 1 14 5 206 5 206 4 17 4 17 2 13 2 93. Primorsko 11 112 11 92 12 472 12 171 17 96 15 71 8 128 1 34. Yasna Poliana 4 57 0 0 21 2074 0 0 29 187 0 0 19 160 0 05. Veselie 8 174 0 0 36 4702 0 0 12 78 0 0 9 43 0 06. Novo Panicharevo 6 97 0 0 22 2647 0 0 43 242 0 0 22 129 0 0Total 36 575 17 172 115 10399 36 481 122 713 33 130 62 479 5 16


V. SURVEILLANCE IN SOZOPOL MUNICIPALITY

49

Fig.17. Location of the settlements under surveillance in Sozopol municipalityTable 1. First blood sampling of FMD susceptible animals in the settlements of Sozopol municipality (14.01.2011)

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken present Samples takenSettlement

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

1. Indje Voyvoda 0 0 0 0 0 0 0 0 0 0 0 0 1 72 1 222. Varshilo 0 0 0 0 0 0 0 0 0 0 0 0 5 777 0 03. Krushovets 0 0 0 0 0 0 0 0 0 0 0 0 8 811 2 53Total 0 0 0 0 0 0 0 0 0 0 0 0 14 1660 3 75


Table 2. Second blood sampling of FMD susceptible animals in the settlements of Sozopol municipality (07.02.2011)Large ruminants sheep goats pigs

present Samples taken present Samples taken present Samples taken present Samples takenSettlement№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals1. Indje 0 0 0 0 0 0 0 0 0 0 0 0 1 72 1 40

50

Voyvoda2. Varshilo 0 0 0 0 0 0 0 0 0 0 0 0 5 777 5 1943. Krushovets 0 0 0 0 0 0 0 0 0 0 0 0 8 811 8 200Total 0 0 0 0 0 0 0 0 0 0 0 0 14 1660 14 434


FMD surveillance in East-Balkan pig herds

Table: East-Balkan pigs herds and samples taken on 07.02.2011

Settlement Owners Holdingidentification

Numberof pigs

№blood

samplesIndze Voyvoda S A D 81490097 72 40

G P S 81480001 120 48V Z Y 81480185 250 48G K 81480012 16 16P H D 81480217 60 38

Krushevets

I H D 81480218 75 50K D Y 81570100 300 54D G F 81570058 25 20

Varshilo

H D A 81570023 30 16

51

Settlement Owners Holdingidentification

Numberof pigs

№blood

samplesIndze Voyvoda S A D 81490097 72 40

G P S 81480001 120 48V Z Y 81480185 250 48G K 81480012 16 16P H D 81480217 60 38

Krushevets

I H D 81480218 75 50K D Y 81570100 300 54D G F 81570058 25 20H D A 81570023 30 16R S Z 81570078 180 52

Varshilo

D I D 81570080 220 52

Fig.18 Location of the 11 East-Balkan pigs herds in Sozopol municipality

Total 1348 434

All samples NSP negative

52

Annex V

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUNDTHE OUTBREAK IN THE VILLAGE OF KIROVO, SREDETS MUNICIPALITY

(Disease confirmed on19th of March) ( Outbreak IP-4)

Fig 20. Location of the infected farm (№IV outbreak-Kirovo) and the distance from the last outbreak (№ 3 outbreak –Gramatikovo)

In the 3 km protection zone are located two villages (Kirovo and Dolno Yabalkovo, Tsarevo municipality). There are 7 villages located in the 10 kmsurveillance zone Belevren, Granichar, Golyamo Bukovo, Trakiitsi, Varovnik, Gorno Yabalkovo and Fakia, Sredets municipality).

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak inKirovo, Sredets Municipality (18.03.11-30.03.2011)

53

Large ruminants sheep goats pigs

present Samples taken presentSamples taken

present Samples takenpresent

Samples takensettlement

№herds

№animals

№herds

№animal

s

№herds

№animals

№herds

№herds

№herds

№animals

№herds

№anima

ls

№herds

№anima

ls

№herds

№animals

Kirovo-bovine farm 1 143 1 42 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 3 km protection zone

1Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 02.DolnoYabalkovo 1 45 1 31 1 69 1 3 1 287 3 45 1 2 1 6

Settlements in the 10 km surveillance zone1. Belevren 1 65 1 17 0 0 0 0 3 7 0 0 0 0 0 02. Granichar 1 133 1 15 0 0 0 0 0 0 0 0 0 0 0 03. GolyamoBukovo 5 319 5 119 2 97 2 97 1 57 1 51 1 11 1 34. Trakiici 0 0 0 1 8 1 5 1 94 1 35 2 88 2 105. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 06.GornoYabalkovo 2 77 0 o 6 348 0 0 6 56 0 0 0 0 0 07.Fakia 5 278 5 220 4 469 4 132 2 288 2 59 0 0 0 0Total 18 1411 16 546 15 998 9 244 16 806 9 194 4 101 4 19

Laboratory results:Kirovo bovine farm – 15 samples from cattle positive for RT PCR, 28 samples positive for Ab ELISAAll samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

54

Annex VI

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUNDTHE OUTBREAK IN THE VILLAGE OF GRANICHAR , SREDETS MUNICIPALITY

(Disease confirmed on 24th of March) ( Outbreak IP-5)

Fig 21. Location of the settlements in the 3km protective and 10 km surveillance zones around Granitchar village, Sredets municipality

In the 3 km protection zone are located two villages (Bogdanovo and Dolno Yabalkovo, Tsarevo municipality). There are 8 villages located in the 10 kmsurveillance zone Belevren, Kirovo, Golyamo Bukovo, Trakiitsi, Varovnik, Sredets municipality , Evrenozovo, Bliznak and Madezhko, Malko Turnovomunicipality).

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak inGranitchar, Sredets Municipality (18.03.11-30.03.2011)

55

Large ruminants sheep goats pigspresent Samples taken present Samples taken present Samples taken present Samples taken

Settlements№

herds№

animals№

herds

№anima

ls

№herds

№animals

№ herds

№animals

№herds

№animal

s

№ herds

№anima

ls

№herds

№animals

№ herds

№animals

1 Granichar –farm (IP 5) 1 133 1 15 0 0 0 0 0 0 0 0 0 0 0 0

Settlements in the 3 km protection zoneBogdanovo 2 374 0 0 3 175 0 0 2 121 0 0 1 41 0 0DolnoYabalkovo 1 45 1 31 1 356 1 5 3 45 1 2 1 6

Settlements in the 10 km surveillance zone1. belevren 1 65 1 17 0 0 0 0 3 7 0 0 0 0 0 02.Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 03. GolyamoBukovo 5 319 5 119 2 97 2 97 1 57 1 51 1 11 1 44. Trakiici 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 105. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 06.evrenezovo 1 10 1 18 1 14 1 14 1 10 1 10 1 23 1 101. Bliznak 5 137 5 96 6 158 6 121 6 111 6 91 2 9 2 68.Mladejko 0 0 0 0 0 0 0 0 9 33 9 33 0 0 0 0Total 18 1434 16 398 15 815 12 249 25 450 23 269 8 174 7 36

Laboratory results:Granichar farm – 11 samples from cattle positive for RT PCR, 15 samples positive for ELISA O typeAll samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

56

Annex VII

SURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUNDTHE OUTBREAKS IN THE VILLAGE OF GOLIAMO BUKOVO, FAKIA, MOMINA TSURKVA, SREDETS MUNICIPALITY

(Disease confirmed on 25th of March in outbreaks IP-6, IP-7, IP-8,)

Fig 22. Location of the three outbreaks ( IP-6, IP-7, IP-8) in Sredets municipality, Burgas region with the 3 km protection and 10 km surveillance zones around the last outbreaks.

GOLIAMO BUKOVO outbreak IP-6 - In the 3 km protection zone are located three villages (Golyamo Bukovo, Kirovo and Trakiitsi, Tsarevomunicipality). There are 6 villages located in the 10 km surveillance zone (Bliznak, Granitchar, Fakia, Varovnik, Dolno Yabulkovo, Bogdanovo, Sredetsmunicipality).

57

FAKIA outbreak IP-7 - In the 3 km protection zone is located one village (Fakia, Tsarevo municipality). There are 6 villages located in the 10 kmsurveillance zone (Gorno Yabalkovo, Dolno Ybalkovo, Kirovo, Golyamo Bukovo, Slivovo, Momina Tsurkva, Sredets municipality)

MOMINA TSURKVA outbreak IP-8 -in the 3 km protection zone is located one village (Momina Tsurkva, Tsarevo municipality). There are 7 villageslocated in the 10 km surveillance zone (Gorno Yabalkovo, Dolno Ybalkovo, Fakia, Sredets municipality, Burgas region, Golyamo Krushevo, Vulchiizvor, Gorska Polyana, Strandzha, Bolyarovo municipality, Yambol region)

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreak inGoliamo Bukovo, Fakia, Momina Tsurkva, Sredets Municipality (18.03.11-30.03.2011)

Large ruminants sheep goats pigs

PresentSamples taken



PresentSamples takenSettlements

№herds

№animals

№ herds

№animals

№herds

№animals

№ herds

№animals

№herds

№animals

№ herds

№animals

№herds

№animals

№ herds

№animals

6 farm- GolyamoBukovo (IP 6) 2 188 2 57 1 11 1 3Fakia- farm(IP 7) 1 81 1 81 0 0 0 0 0 0 0 0 0 0 0 0Momina Tsurkvaoutbreak (IP 8) 1 209 1 11

Settlements in 3km protective zones around the three outbreaksGolyamoBukovo 3 131 3 62 2 97 2 97 1 57 1 51Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 0Trakiici 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10Fakia 4 197 4 122 4 469 4 132 2 288 2 59 0 0 0 07.Mominacarkva 3 133 2 53 5 2194 5 287 3 161 3 60 1 57 1 10

Settlements in 10km surveillance zones around the three outbreaks1. Bliznak 5 93 5 71 6 89 6 62 6 92 6 36 2 9 2 62. Granichar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 04. Varovnik 1 216 1 50 0 0 0 0 0 0 0 0 0 0 0 0

58

5.DolnoYabalkovo 1 45 1 31 1 85 1 26 4 279 4 80 1 6 1 46.Bogdanovo 2 374 2 299 3 254 3 175 2 121 2 121 1 41 1 411. GornoYabalkovo 0 0 2 77 6 348 2 56 0 0 0 03. Kirovo 1 135 1 52 1 7 1 7 2 17 2 4 0 0 0 06.Slivovo 1 31 1 29 2 154 1 70 0 0 0 0 0 04. GolyamoKrushevo

0 0 0 0 10 504 1 28 20 305 15 28 0 0 0 05. valchi izvor

0 0 0 0 1 45 1 20 6 80 1 23 0 0 0 06.GorskaPolyana

2 109 2 25 6 60 2 21 10 80 1 23 0 0 0 07.Strandzha 4 407 2 28 5 80 2 27 4 50 1 6 0 0 0 0Total 32 2072 31 1512 54 4398 30 897 66 1695 41 602 8 126 8 160

Note:* Granichar - all susceptible animals in the village were killed and buried by 26.03.2011, because detection of FMD outbreak in the village on 24.03.2011.

The laboratory results are:

Goliamo Bukovo –Cattle:11 samples positive for RT-PCR , 3 of them positive for FMD Ag ELISA, all of them negative for Mono - O type ELISA Prionics

FakiaCattle- 6 positive for RT-PCR and 1 sample positive for FMD Ag ELISA

Momina Tsarkvacattle – 6 positive for RT-PCR, 2 positive for FMD Ag ELISA.All other samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics).

59

Annex VIIISURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND

THE OUTBREAKS IN THE VILLAGE OF BLIZNAK , MALKO TURNOVO MUNICIPALITY(Disease confirmed on 3rd of April) ( Outbreaks IP-9 and IP-10)

12

)Fig 23. Location of the settlements in the 3km protective and 10 km surveillance zones around the outbreaks ( №IX and X) in Bliznk village, Malko Turnovo municipality

In the 3 km protection zone there are no villages.There are 5 villages located in the 10 km surveillance zone (Evrenozovo, Mladezhko and Zvezdets,Malko Turnovo municipality, Bogdanovo, Varovnik, Trakiitsi and Granitchar, Sredets municipality, Burgas region

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreaks inBliznak, Sredets Municipality (18.03.11-30.03.2011).

Large ruminants sheep goats pigsSettlements




PresentSamples

taken

60

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animals

№herds

№animal

s

№herds

№anima

ls

№herds

№animal

sBliznak village(IP 9) 4 21 5 21 5 69 5 42 5 42 5 36 2 9 2 6

Bliznakbuffalo herd(IP 10 1 72 1 57 1 20 1 20 1 52 1 36 0 0 0 0

Settlements in 10km surveillance zones around the three outbreaksEvrenozovo 1 24 1 12 1 51 1 14 1 39 1 10 1 39 1 23Mladezhko 0 0 0 0 0 0 0 0 9 97 9 33 0 0 0 0Zvezdets 6 297 6 0 9 187 9 106 3 94 3 63 3 44 3 44Bogdanovo 2 374 0 0 3 175 3 175 2 121 2 121 1 41 0 0Varovnik 1 233 1 0 0 0 0 0 0 0 0 0 0 0Granichar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Trakiitsi 0 0 0 0 1 8 1 5 1 94 1 35 2 88 2 10Total 15 1021 14 90 20 589 20 362 22 550 22 334 9 221 8 83

The laboratory results are as follow:Bliznak village

Cattle - 21 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISASheep - 42 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISAGoats 36 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISA

Buffalo FarmBuffaloes – 56 samples positive for FMD ELISA Type O Priocheck , 43 positive for FMD NS ELISASheep - 20 samples positive for FMD ELISA Type O Priocheck and FMD NS ELISAGoats - 36 positive for FMD ELISA Type O Priocheck and FMD NS ELISA.

All other samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics) and FMD NS ELISA

61

Annex IXSURVEILLANCE IN THE 3km PROTECTION AND 10km SURVEILLANCE ZONES ARROUND

THE OUTBREAK IN THE VILLAGE OF DOLNO YABULKOVO, SREDETS MUNICIPALITY(Disease confirmed on 7th of April) (Outbreak IP-11)

Fig24. Location of the settlements in the 3km protective and 10 km surveillance zones around Dolno Yabalkovo village, Sredets municipality

In the 3 km protection zone is located one village (Gorno Yabalkovo, Tsarevo municipality). There are 6 villages located in the 10 km surveillance zone(Momina Tsurkva, Fakia, Golyamo Bukovo, Kirovo, Belevren, Granitchar, Sredets municipality, Burgas region)

62

Table 1. Blood sampling of FMD susceptible animals in the settlements in the 3 km protection and 10 km surveillance zones around the outbreaks inBliznak, Sredets Municipality (07.04.11-15.04.2011)

Large ruminants sheep goats pigsPresent Samples taken Present Samples taken Present Samples taken Present Samples taken

Settlements №

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals№

herds№

animals

№herd

s

№animals

№herds

№anima

ls

№herds

№animal

sDolnoYabulkovo

1 45 1 35 1 85 1 26 4 279 4 80 1 6 1 4

Settlements in 3km protective zones around the outbreakGornoYabalkovo 2 129 2 77 6 546 6 348 2 87 2 52 0 0 0 0

Settlements in 10km surveillance zones around the outbreak2.MominaTsurkva 2 85 2 57 5 2194 5 752 3 232 3 161 1 57 0 03. Fakia 4 197 4 118 4 132 4 83 2 59 2 32 0 0 0 04.GolyamoBukovo 3 131 3 102 2 97 2 90 1 57 1 51 0 0 0 05. Kirovo 1 135 1 135 1 7 1 7 2 17 2 10 0 0 0 06.Belevren 1 65 1 65 0 0 0 0 3 7 3 7 0 0 0 07.Granichar 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0Total 14 787 14 532 21 3061 19 1306 17 738 17 393 2 63 1 4

The laboratory results are as follow:Dolno Yabulkovo:

Cattle -2 positive for Mono ELISA O-type Prionics.Goats/sheep -22 positive for Mono ELISA O-type Prionics

All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

63

Annex XSurveillance in domestic livestock (18.04-19.04.2011)

I. Clinical examinations and serological surveillance of susceptible animals in the settlements located in 3 km protective and 10 km surveillance zonesaround the outbreaks of the second wav e(March –April)

Fig 25. Settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table 1. Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks (second outbreak wave)

64

Animalholdings Animals in the settlements

Municipalities Settlements

num

ber (№

)

cattl

e

Shee

p

goat

s

Buf

falo

Pigs

Totalnumber of

animals

№ ofblood

samplestaken to

detect 5 %prevalencewith 95%

confidencewithinvillage

№ ofblood

samplestaken

№ ofblood

samplestaken peranimals

Settlements in 3 km protective zones around the outbreaks IT- 4 to IT-11

Kirovo 4 135 7 17 0 0 159 49 72bovine-48sheep -7goats-17

GolyamoBukovo 14 134 94 51 0 0 319 54 103

bovine-54sheep-42goats-7

Fakiya 64 180 469 288 38 0 975 57 302

bovine-128

sheep-67goats-107

Trakiitsi 4 8 94 0 88 180 50 67sheep-7goats-46pigs-14

Granichar* 0 0 0 0 0 0 0 0 0 0

GornoYbulkovo 10 77 348 52 0 0 477 55 225


DolnoYabalkovo* 0 0 0 0 0 0 0 0 0 0

Sredetsmunicipality,Burgas region

MominaTsurkva 66 57 2194 161 0 57 2412 58 274


MalkoTurnovo Bliznak* 0 0 0 0 0 0 0 0 0 0

65

Evrenozovo 2 12 14 10 0 23 59 38 44

goats-10,sheep-11,bovine-4,pigs-19

settlements in 10 km surveillance zones the outbreaks IT- 4 to IT-11

Bogdanovo 22 375 175 121 0 41 671 57 307

bovine-102

sheep-132goats-73

Varovnik 2 216 0 0 0 0 216 51 90bovine-90bovine-53

goats-6

Belevren 4 74 0 7 0 0 81 42 59bovine-90bovine-53

goats-6

Vulchanovo 12 118 199 157 0 0 474 55 60bovine-12sheep-33goats-15


Slivovo 8 31 158 70 0 0 259 53 132bovine-26sheep-87goats-19

Zvezdets 15 146 106 63 79 44 453 56 72 bovine-72MalkoTurnovo

municipality,Burgas region Mladezhko 9 0 0 40 0 0 40 31 32 goats-32

Golyamokrushevo 30 0 504 305 0 0 809 57 58 sheep-28

goats-30

Gorskapolyana 18 109 60 80 0 0 249 53 72

bovine--28sheep-21goats-23

Bolyarovomunicipality,Burgas region

Strandzha 13 407 80 50 0 0 537 56 61bovine--28sheep-27goats-6

66

Total 20villages 297 2071 4416 1566 117 253 8370 872 2030

Note – no susceptible animals in Granichar, Bliznak, Dolno Yabalkovo because detection of FMD outbreak in these villages ( outbreaks IP-5, IP-10 and IP 11)All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

II. Serological surveillance in the villages in the risk municipalities of Burgas and Yambol regions as well as in other villages in Burgas, Varna, Shumen,Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detectedoutbreaks

67

FMD case in wild boarFirst FMD outbreak wave

Settlements where the blood sampling inthe susceptible animals were carried out

Second FMD outbreak wave

FMD case in wild boarFirst FMD outbreak wave

Settlements where the blood sampling inthe susceptible animals were carried out


Fig 26 -Blood sampling in the susceptible animals in the settlements located in the regions outside and around the 3 km protective and 10 km surveillance zones around the secondoutbreak wave included in Annexes I of Decision2011/44/ЕС.

Table 2 – Blood sampling in the susceptible animals in the settlements located in the regions outside and around the 3 km protective and 10 kmsurveillance zones around the second outbreak wave included in Annexes I of Decision2011/44/ЕС.

Municipalities Settlements East Balkanpigs


Totalnumber

of

№ ofblood

samples

№ ofblood

samples

№ of blood samplestaken per animals

68

Total number of animals

num

ber

(№) o

fho

ldin

gsnu

mbe

r(№

) of

anim

als

num

ber

(№)

cattl

e

Shee

p

goat

s

Buf

falo

Pigs

animals taken todetect 5 %prevalencewith 95%


taken

Burgas

Sredec Debelt-1 0 0 57 1 984 219 0 59 1263 57 60 sheep-60

Sredec Dyulevo-2 0 0 58 287 1592 91 0 8 1978 58 54 bovine-15;sheep-29;goat-10

Sredec Zagorci- 3 0 0 31 319 532 36 0 0 887 57 60 bovine-16;sheep-43;goat-1

Sredec Zornitsa- 4 0 0 66 102 150 303 0 0 555 56 60 bovine-16;sheep-43;goat-1

Sredec Granitec- 5 0 0 8 16 164 37 0 0 217 51 60 bovine-5;sheep-55

Sredec SinyoKamenene-6 0 0 6 260 294 158 0 0 712 57 60 bovine-25;sheep-

33;goat-2

Sredec Valchanovo-7 0 0 12 118 199 157 0 0 474 55 60 bovine-12;sheep-

33;goats-15

Sozopol Dimchevo- 8 0 0 20 25 27 86 0 0 118 45 48 bovine-12,sheep-10,goats-26

Sozopol Marinka- 9 0 0 97 12 610 198 0 0 820 57 194 bovine8,sheep152,goats-34

69

Sozopol Rosen-10 0 0 57 38 392 79 0 60 569 56 123

bovine31,sheep-55,goats-19,pigs-18

Sozopol Ravdinovo-11 0 0 32 102 792 184 4 26 1108 57 134 bovine-41,sheep-

59,pigs-9,goats-25

Karnobat Iskra- 12 0 0 32 32 1054 17 0 0 1103 57 156bovine,sheep,goats

Karnobat Klikach- 13 0 0 84 75 928 10 0 0 1013 57 164bovine,sheep,goats

Aytos Pirne-14 0 0 138 432 1710 85 - 174 2401 58 208

bovine-60sheep-60goats-50pigs-38

Aytos Sadievo- 15 0 0 68 71 890 45 - - 1006 57 115bovine-50sheep-50goats-15

Aytos Chukarka- 16 0 0 146 95 1000 80 - 15 1190 57 146

bovine-50sheep-60goats-30pigs-6

Kameno Vinarsko-17 0 0 31 60 98 22 0 6 186 50 100 bovine-29, sheep-43,goats-22, pigs-6

Burgas Balgarovo-18 0 0 104 113 1087 219 0 0 1419 58 128 bovine-45,sheep-

52,goats-31

Pomorie Kableshkovo-19 0 0 22 2 1600 36 0 18 1656 58 90 bovine-2; sheep-60;

goats-10; pigs-18

Nesebar Tankovo- 20 21 20 16 125 3000 24 0 0 3149 58 137 bovine-47; sheep-63;goats-7; pigs-20

70

Total 20 villages 21 20 1085 2285 17103 2086 4 366 21824 1116 2157


Fig 27 – location of the settlements in the regions outside the 3 km protective and 10 km surveillance zones zones around the second outbreak wave andincluded in Annex II of Decision2011/44/ЕС. (Varna, Shumen, Sliven, Yambol and Haskovo)

FMD case in wild boar

First FMD outbreak wave


Settlements where the blood sampling in thesusceptible animals were carried out

71

Table 3 – Blood sampling in the susceptible animals in the settlements located in the regions outside the 3 km protective and 10 km surveillance zoneszones around the second outbreak wave and included in Annex II of Decision2011/44/ЕС.

Animals in the settlements

East Balkan pigs Animalholdings

Total number of animalsMunicipalities Settlementsnu

mbe

r(№

) of

hold

ings

num

ber

(№) o

fan

imal

s

num

ber

(№)

cattl

e

Shee

p

goat

s

Buf

falo

Pigs

Totalnumber

ofanimals

№ ofblood

samplestaken to



№ ofblood

samplestaken

№ ofblood


Shumen region

Varbitsa Varbitsa- 21 0 0 244 152 508 105 0 0 765 57 57bovine 14sheep35goats 10

Varbitsa Byala reka-22 0 0 287 258 1009 30 0 0 1297 58 58

bovine 16sheep 35goats 12

Smyadovo Rish-23 97 53 197 61 1590 205 0 0 1856 58 58bovine 10sheep 25goats 23

Smyadovo Veselinovo-24 842 229 151 146 187 71 0 0 404 55 55

bovine 8sheep 30goats 17

Varna region

72

Dalgopol Partizani-25 0 0 262 100 600 200 0 27 1527 121 122

Bovine45, sheep

43 ,goats-15,pigs 19

Dalgopol Asparuhovo-26 0 0 123 0 140 55 0 0 195 50 50

Sheep44,goats

6

Dolni Chiflik Golitsa-27 150 50 217 30 600 100 0 0 880 134 135

Bovine26,sheep52, goats7,pigs 50

Dolni Chiflik Yunety- 28 0 0 0 0 0 0 0 0 0 0 0 0

Sliven region

Kotel Kotel- 29 0 0 22 369 986 170 0 0 1525 112 112

54-bovine

57 sheep1 goats

Sliven Ichera- 30 0 0 9 40 326 217 0 60 643 127 127

31-bovine

54 Sheep4 goats38Pigs

Sliven Samuilovo-31 0 0 46 1242 2800 0 0 0 4042 116 116

58-bovine

58-Sheep

Sliven Kermen- 32 0 0 80 736 3720 0 0 28 4484 139 139

57-bovine

58 Sheep24Pigs

Yambol region

73

Straldja Vodenichene-33 0 0 45 114 211 63 0 920 1308 58 154

bovine -44

Sheep-29Goats-22Pigs-59

Straldja Tamarino- 34 0 0 51 34 1220 60 0 0 1365 58 99

bovine -34

Sheep-27Goats-38

Straldja Kamen vrah-35 0 0 6 0 21 19 0 0 40 31 37 Sheep-18

Goats-19

Bolyarovo Mamarchevo-36 0 0 40 18 53 31 0 0 597 56 102

bovine -18

Sheep-53Goats-31

Elhovo Golyamdervent- 37 0 0 11 1 38 19 0 0 130 48 58

bovine -1,Sheep-38,Goats-

19

Tundja Roza-38 0 0 81 291 600 180 0 30 1182 57 188

bovine -56

Sheep-56Goats-50Pigs-26

Haskovo region

Topolovgrad Knyajevo- 39 0 0 0 22 401 132 0 0 555 133 133

goats-52sheep-59bovine-

22

Svilengrad Varnik-40 0 0 4 56 67 18 0 1 142 48 80bovine-

38sheep-42

Svilengrad Matochina-41 0 0 21 37 240 43 0 3 323 54 84bovine-

31sheep-53

74

Total 20 villages 1089 332 1897 3707 15317 1718 0 1069 23260 1570 1964


75

Annex XIClinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions for

proving absence of FMD virus circulation in domestic livestock in the areas north and west of the detected outbreaks.(28 - 29 .04.2011)

Fig 28-settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table – Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks (second outbreak wave)Municipalities Settlements Animal

holdings Animals in the settlementsTotal

number ofanimals

№ ofblood

samplestaken to

№ ofblood

samplestaken

№ ofblood

samplestaken per

76

num

ber (№

)

cattl

e

Shee

p

goat

s

Buf

falo

Pigs


Kirovo 4 150 7 17 0 0 174 50 65cattle-45sheep-7goats-13

GolyamoBukovo 14 145 209 62 0 0 416 55 118


Fakiya 64 180 469 288 38 0 975 57 467bovine-

113sheep-176goats-178


Granichar* 0 0 0 0 0 0 0 0 0 0

GornoYbulkovo 10 84 370 52 0 0 506 56 147

bovine-30sheep-116

goats-1



MominaTsurkva 66 57 2194 161 0 57 2412 58 141

bovine-11sheep-128

goats-2

Malko Bliznak* 0 0 0 0 0 0 0 0 0 0

77

Turnovomunicipality,Burgas region Evrenozovo 2 12 14 10 0 23 59 38 0 0


Bogdanovo 22 375 175 121 0 41 712 57 258bovine-

101sheep-109goats-48

Varovnik 2 216 0 0 0 0 216 51 86 bovine-86

Belevren 4 77 0 7 0 0 84 42 59 bovine-53goats-6

Vulchanovo 0 0 0 0 0 0 0 0 0 0



Zvezdets 15 146 106 63 79 44 453 56 56 bovine-56MalkoTurnovo

municipality,Burgas region Mladezhko 9 0 0 40 0 0 40 31 31 goats-31

Golyamokrushevo 30 0 504 305 0 0 809 57 56 sheep28

goats28

Gorskapolyana 18 109 60 80 0 0 249 53 72

bovine 28sheep21goats23


Strandzha 13 407 80 50 0 0 537 56 61bovine -28sheep-27goats- 6

78

Total 20villages 285 1989 4357 1431 117 253 8105 821 1813


79

Annex XIIClinical examinations and blood sampling of susceptible animals in the villages in the risk municipalities of Burgas and Yambol regions as wellas in other villages in Burgas, Varna, Shumen, Sliven, Yambol and Haskovo regions for proving absence of FMD virus circulation in domestic

livestock in the areas north and west of the detected outbreaks(18 - 19.05.2011)

Fig 29-settlements located in 3 km protective and 10 km surveillance zones around the second outbreak wave.

Table 1. Blood sampling in the susceptible animals in the settlements located in 3 km protective and 10 km surveillance zones around the outbreaks (second outbreak wave)

80


Municipalities Settlements

num

ber (№

)

cattl

e

Shee

p

goat

s

Buf

falo

Pigs

Totalnumber of

animals

№ ofblood

samplestaken to



№ ofblood

samplestaken

№ ofblood



Kirovo 4 150 7 17 0 0 174 50 47bovine-30sheep-4goats-13

GolyamoBukovo 14 145 209 62 0 0 416 55 121


Fakiya 64 180 469 288 38 0 975 57 213bovine-83sheep-71goats-59


Granichar* 0 0 0 0 0 0 0 0 0 0

GornoYbulkovo 10 84 370 52 0 0 506 56 155

bovine-35sheep-115

goats-5



MominaTsurkva 66 57 2194 161 0 57 2412 58 203


MalkoTurnovo Bliznak* 0 0 0 0 0 0 0 0 0 0

81

Evrenozovo 2 12 14 10 0 23 59 38 26

bovine-4,goats-

10.sheep-12


Bogdanovo 22 375 175 121 0 41 712 57 165bovine-60sheep-64goats-29pigs-12

Varovnik 2 216 0 0 0 0 216 51 55 bovine-55

Belevren 4 77 0 7 0 0 84 42 42 bovine-36goats-6

Vulchanovo



Zvezdets 15 146 106 63 79 44 453 56 297

bovine-88,bufolloes-40,goats-46, sheep-87,pigs-36

MalkoTurnovo

municipality,Burgas region

Mladezhko 9 0 0 40 0 0 40 31 31 goats-31


Golyamokrushevo 21 0 504 305 0 0 809 57 56 sheep28

goats28

82

Gorskapolyana 18 109 60 80 0 0 249 53 68

bovine -25sheep-20goats-23

Strandzha 13 418 80 50 0 0 548 56 82bovine -36sheep-27goats-19

Total 20villages 276 2000 4357 1431 117 253 8116 821 1695

Note – no susceptible animals in Granichar, Bliznak, Dolno Yabalkovo because detection of FMD outbreak in these villages ( outbreaks IP-5, IP-10 and IP 11)All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

83

Fig 30 –– Blood sampling in the susceptible animals in the settlements located in the regions outside the 3 km protective and 10 km surveillance zones around the second outbreakwave included in Annexes II of Decision2011/44/ЕС (regions of Varna, Shumen, Sliven, Yambol and Haskovo)

Table 2 – Blood sampling in the susceptible animals in the settlements located in the regions around the 3 km protective and 10 km surveillance zones around the second outbreakwave and included inAnnex II of Decision2011/44/ЕС

Animals in the settlementsEast Balkan pigs Animal

holdings

Total number of animalsMunicipalities Settlements

num

ber

(№) o

fho

ldin

gs

num

ber

(№) o

fan

imal

s

num

ber

(№)

cattl

e

Shee

p

goat

s

Buf

falo

pigs

Totalnumber

ofanimals

№ ofblood

samplestaken to



№ ofblood

samplestaken

№ of bloodsamplestaken peranimals

Burgas regionNesebar Priseltsi- 1 0 0 8 0 211 200 0 0 411 55 100 sheep-45,

goats-55Ruen Rojden- 2 0 0 0 120 419 0 0 0 539 56 100 bovine-50;

sheep-50Sungurlare Kamensko- 3 0 0 9 127 114 0 0 0 241 51 97 bovine;sheep

Varna region

Byala Dulino- 4 77 0 80 30 0 0 0 0 140 72 77EBP 30-sheep 23,goats 24

FMD case in wild boar

First FMD outbreak wave


Settlements where the blood sampling in thesusceptible animals were carried out

84

Dolni chiflik Bulair- 5 0 49 119 334 20 0 0 49 473 101 10147

bovine,sheep48 ,goats 6

Shumen region

Smyadovo Aleksandrovo-6 0 0 39 89 30 30 0 221 350 54 54

bovine 17sheep 13goats 10pigs 14

Varbitsa Kraigortsi- 7 0 0 67 50 286 7 0 0 343 54 54bovine 22sheep 25.goats 7

Sliven region

Kotel Gradtes- 8 0 0 5 64 0 183 0 0 247 90 90 40-bovine50 goats

Yambol region

Straldja Malenovo- 9 0 0 33 378 420 42 0 0 840 57 135bovine -55sheep-60goats-20

Lyuilin- 10 0 0 40 77 110 32 0 0 219 51 84bovine -40sheep-33goats-11

Bolyarovo Valchi izvor-11 0 0 7 0 45 80 0 0 125 47 43 sheep-20

goats-23Haskovo region

Topolovgrad Radovets- 12 0 0 0 91 488 290 0 0 869 150 150sheep-60goats-50

bovine-40

Svilengrad Shtih -13 0 0 41 4 220 60- 0 11 295 54 54bovine-3sheep-36goats-15

Svilengrad Chernodab-14 0 0 6 26- 0 0 0 0 26 23 23 bovine-23

Total 14-villages 77 49 454 1390 2363 924 0 281 5118 915 1162All samples are negative for antibody to SP of the FMDV (Mono ELISA O-type Prionics)

85

Annex XIIIClinical surveillance of susceptible livestock in the”Cordon Sanitaire” (16 - 25.08.2011)

Fig.31 Map with the location of the three sampling areas within “Cordon Sanitaire”: Sampling area A - Infected area (Tsarevo-south part (1) and Malko Tarnovo (2) and Sredets -south part (5’) municipalities) and Sampling area B - risk area (Tsarevo-north part (1’) Primorsko (3), Sozopol (4), Sredets -central part of (5’), and Sampling area C - risk area (partsof Bolyarovo (6), Elhovo (7), Topolovgrad (8), Svilengrad (9) municipalities)

Table – Blod sampling in the susceptible animals in the settlements located in the area”Cordon Sanitaire”.

86

Animals in the settlements

Municipalities Settlements VillageStatus

Num

ber

of a

nim

alho

ldin

gs

catt

le

Shee

p

goat

s

Buf

falo

pigs

Tot

al n

umbe

r of

ani

mal

s № ofblood

samplestaken to

detect 5 %prevalencewith 95%confidence

withinvillage

№ of bloodsamplestaken

№ ofblood


Burgas region

Ahtopol A 28 127 1 752 230 0 128 2237 58 5810-bovine,20-sheep,20-goats,

8-pigs

Brodilovo A 56 9 392 672 0 43 1116 57 57

5-bovine,24-sheep,20-goats,

8-pigs

Balgari A 7 0 146 34 0 0 180 50 43 35-sheep,8-goats

Varvara A 15 33 308 186 0 0 527 56 5614-bovine,26-sheep,16-goats

Kosti* A 0 0 0 0 0 0 0 0 0 0

Tsarevo

Rezovo* A 0 0 0 0 0 0 0 0 0 0

87

Velika Б 5 61 100 104 0 0 265 53 5313-bovine,15-sheep,25-goats

Izgrev A 3 0 8 0 0 12 20 19 6 6-pigs

Kondolovo A 3 0 144 98 0 0 242 52 52 25-sheep,27-goats

Lozenec Б 16 7 483 516 0 15 1021 57 57 20-sheep,37-goats

Sinemorets A 12 17 376 95 0 9 497 56 568-bovine,24-sheep,24-goats

Fazanovo Б 7 162 762 14 0 46 984 57 57 20-bovine,37-sheep

Tsarevo A 38 74 3 232 319 0 0 3625 58 5710-bovine,30-sheep,17-goats

Varshilo B 26 260 218 192 10 697 1377 58 58

12-bovine,3-бв, 11-sheep, 7-goats, 25-

pigs

Gabar B 6 1 660 110 0 0 771 57 57 10-goats,47-sheep

Sozopol

Zidarovo B 182 243 884 714 0 263 2104 58 58

5-bovine,24-sheep,18-goats,11-pigs

88

Izvor B 185 4 3 605 559 0 241 4409 59 5946-sheep,10-goats,

3-pigs

Indzhe Voyvoda B 43 16 424 481 0 88 1009 57 542-bovine,23-sheep,29-goats

Krushevets B 81 309 1 518 460 0 354 2641 58 58


6-pigs

Belevren A 5 76 0 18 0 0 94 43 43 36-bovine,7-goats

Bogdanovo B 22 171 347 221 0 41 780 57 57

20-bovine,20-sheep,9-goats, 8-

pigs

Varovnik B 3 219 0 0 0 11 230 53 53 52-bovine,1-pigs

Valchanovo B 39 113 334 272 0 0 719 57 5715-bovine,25-sheep,17-goats

GolyamoBukovo A 15 131 137 96 0 3 367 54 54

16-bovine,17-sheep,21-goats

GornoYabalkovo A 10 77 612 108 0 0 797 57 57

16-bovine,37-sheep,7-goats

Sredets

Granitez B 13 16 275 72 0 0 363 55 567-bovine,37-sheep,12-goats

89

Granichar* A 0 0 0 0 0 0 0 0 0 0

DolnoYabalkovo* A 6 0 0 0 0 0 0 0 0 0

Drachevo B 48 385 251 263 0 143 1042 57 57

27-bovine,13-goats,11-sheep,

6-pigs

Kirovo A 4 147 11 28 0 186 51 5140-bovine,5-sheep,6-goats

Momina Carkva A 66 57 2 925 283 0 57 3322 58 5812-bovine,36-sheep,10-goats

Panchevo B 12 159 882 117 0 12 1170 57 57

23-bovine,7-goats,

23-sheep,4-pigs

Rosenovo B 14 137 169 142 0 0 448 55 5520-bovine,19-goats,16-sheep

Sinyo Kamenovo B 9 260 507 160 0 0 927 57 5714-bovine,19-goats,24-sheep

Slivovo B 12 36 292 159 0 0 487 56 5610-bovine,20-sheep,26-goats

Trakiici A 4 0 14 113 0 89 216 51 515-sheep,39-goats,

7-pigs

90

Fakia A 64 198 722 550 38 0 1508 58 5824-bovine,10-sheep,24-goats

NovoPanicharovo Б 61 148 2 163 141 0 59 2511 58 58 18-bovine,

40-sheep

Pismenovo Б 20 14 104 23 0 9 150 49 5010-bovine,33-sheep,7-goats

Primorsko

Yasna Polyana Б 61 58 1 315 123 20 43 1559 58 58 18-bovine,40-sheep

Bliznak* А 0 0 0 0 0 0 0 0 0 0

Brachlyan А 3 6 0 0 0 0 6 6 6 6-bovine

Byala voda А 4 6 46 82 0 44 178 51 51


6-pigs

Vizica А 6 44 148 4 0 0 196 51 5115-bovine,

1-goats,35-sheep

Gramatikovo* А 0 0 0 0 0 0 0 0 0 0

MalkoTarnovo

Evronozowo А 2 11 14 22 0 28 75 34 34

5-bovine,13-sheep,9-goats, 7-

pigs

91

Zabernovo А 9 0 11 175 0 73 259 54 5411-sheep,39-goats,

4-pigs

Zvezdec А 15 226 89 102 79 32 528 56 56


5-pigs

Kalovo А 5 0 57 59 0 44 160 49 4925-sheep,20-goats,

4-pigs

Malko Tarnovo А 75 132 1 654 479 0 165 2430 58 5825-sheep,15-goats,

8-pigs

Mladezko А 9 0 0 80 0 0 80 31 31 31-goats

Slivarovo А 4 0 71 65 0 7 143 45 45 20-goats,25-sheep

Stoilovo А 23 0 106 149 0 0 255 55 55 35-goats,20-sheep

Yambol Region

Bolyarovo C 69 220 843 213 0 20 1296 57 57bovine-17goats-10sheep-30

Voden C 20 160 1 220 173 0 10 1563 58 58Small

ruminants-58

Bolyarovo

Valchi izvor C 10 0 45 80 0 0 125 47 47 sheep-24goats-23

92

GlyamoKrushevo C 20 0 504 350 0 60 914 57 57 sheep-57

Gorska polyana C 10 38 60 80 0 0 178 50 50bovine-35sheep-11goats-4

Denitsa C 16 0 55 50 0 3 108 45 45 sheep-27goats-18

Dabovo C 20 116 107 95 0 0 318 54 54bovine-35goats-10sheep-9

Zlatinitsa C 30 96 296 15 0 2 409 55 55 sheep-55bovine -49

Iglika C 5 122 40 0 0 0 162 49 50 bovine -49

Kraynovo C 10 62 476 35 0 0 573 56 56

bovine -5small

ruminants-51

Malko Sharkovo C 9 457 100 80 0 5 642 56 56small

ruminants-56

Mamarchevo C 25 60 425 95 0 10 590 56 56 goats-56

Oman C 15 122 100 20 0 3 245 53 53bovine-38sheep-8goats-7

Popovo C 130 459 270 166 0 5 900 57 57 bovine-57

93

Ruzhitsa C 12 71 0 120 0 0 191 51

44/the totalnumber vanimals

within thevillage/

goats-44

Sitovo C 14 422 37 71 0 0 530 56 56 bovine -56

StefanKaradzhovo C 80 95 800 357 0 12 1264 57 57

bovine -10sheep-32goats-15

Strandzha C 9 350 85 62 0 0 497 56 55 bovine -15sheep-40

Sharkovo C 15 67 1 220 123 1 10 1421 58 58 sheep-58

Valcha polyana C 31 78 297 0 0 0 375 55 55Bovine -

11Sheep -44

Golyam derventC 13 1 61 68 0 0 130 48 48

Smallruminants-

48

Granitovo C 43 70 1 556 93 0 15 1734 58 58Bovine-4Goats-4

Sheep-50

Lakovo C 25 317 411 106 0 0 834 57 57bovine-21Goats-6

Sheep-30

Elhovo

Lesovo C 130 192 383 941 0 5 1521 58 58 Bovine-7Goats-51

94

Malko Kirilovo C 8 93 87 11 0 0 191 51 51 Bovine-25sheep-26

Malomirovo C 36 336 162 267 0 14 779 57 57

Bovine -22

Sheep-33Pigs-2

Melnitsa C 52 360 310 253 0 28 951 57 57

Bovine -19

Goats-17Sheep-19

Pigs-2

Razdel C 63 211 185 457 0 2 855 57 57

Bovine -13

Sheep-42Pigs-2

Slaveikovo C 10 226 0 26 0 4 256 53 53

Bovine -32

Goats-20Pigs-1

Chernozem C 19 235 338 195 5 22 795 57 57Bovine -

12Sheep-45

Haskovo region

Knyazhevo C 41 19 401 132 0 0 552 56 56Sheep-41Bovine-3Goats-12

Planinovo C 15 31 60 120 0 0 211 51 51Sheep 12

Bovine-10Goats-29

Topolovgrad

Prisadetz C 2 53 0 5 0 0 58 35 35 Bovine-35

95

radovets C 152 91 488 290 0 0 869 57 57Sheep-27Bovine-6Goats-24

Srem C 41 65 708 219 0 0 992 57 57Sheep-36Bovine-5Goats-16

Ustrem C 152 492 949 636 0 0 2077 58 58Sheep-28Bovine-16Goats-14

Filipovo C 3 83 5 0 0 0 88 42 42 Bovine-42

Varnik C 4 56 67 18 18 0 142 48 48

Sheep-22;Bovine-

17,Goats-9

Generalovo C 13 12 16 2 0 0 30 26 26 Sheep-20Bovine-6

Dervishkamogila C 14 5 67 72 0 9 153 48 48

sheep-15Bovine-3Goats-30

Dimitrovche C 82 5 46 233 0 48 332 54 54Sheep-10Bovine-3Goats-41

KapitanAndreevo C 107 46 245 62 0 40 393 55 55

Sheep-29Bovine-10Goats-16

Levka C 135 399 343 201 0 32 945 57 57Sheep-30Bovine-17Goats-10

Svilengrad

Matochina C 21 37 240 43 0 3 323 54 54Sheep-36Bovine-15

Goats-3

96

Mihalich C 42 2 121 94 0 10 227 51 51 Sheep-31Goats-20

Mustrak C 91 16 0 279 0 5 300 54 54 Bovine-6Goats-48

Pashov C 9 81 74 0 2 157 49 49 Sheep-30Bovine-19

Ravna Gora C 6 3 67 16 0 0 86 42 42 Sheep-37Goats-5

Raikova mogila C 110 0 189 158 0 15 362 54 54 Sheep-23goats-31

Sladun C 52 0 47 157 0 11 215 51 51 Sheep-20Goats-31

Studena C 252 137 1 022 743 23 99 2024 58 58Sheep-35Bovine-8Goats-15

Shernodab C 6 11 40 0 0 3 54 35 35 Bovine-10Sheep-25

Shtit C 41 4 220 60 0 11 295 53 53Sheep-30Bovine-2Goats-21

Total 106 villages 3696 10834 44156 17032 194 3274 75443 5232 5203

Note – no susceptible animals in Kost, Rezovo, Gramatikovo, Granichar, Bliznak, Dolno Yabalkovo because detection of FMD outbreak in these villages ( outbreaks IP-a, IP-2, IP-3,IP-5, IP-10 and IP 11)


97

Annex XIVSurveillance in wildlife; January – August 2011

Fig 32 -the location of the traps for wild boar in Tsarevo and Malko Tarnovo municipalities

98

Fig 33 -surveillance in wildlife – January –August 2011

Table. Serological surveillance in wildlife in the period January –August 2011)Number of sampled Ab positive FMDV positive

wild boar (Sus scrofa) 89 2 0

roe deer (Capreolus capreolus) 36 3 0

red deer (Cervus elaphus) 2 0 0

Total 127 5 0

99

Annex XVTime of FMD events in Bulgaria 2010 - 2011

ENTRY TO IP3 1ST LESIONS-IP3SPREAD WITHIN KOSTI VILLAGE (=IP 2).LEADS TO 27 +VE AT SAMPLING ON 7TH

IP1 infectious - spread

28 N 30thN 2 De 4 De 6 De 8 De 10 De 12 De 14 De 26De 28De 30De 2Ja 4Ja 6Ja 8Ja 10Ja 12Ja 14Ja 18Ja 20Ja 22Ja

MINIMUM PERIOD OF INFECTIOUS WILD BOAR (SUSPECTED AND CONFIRMED CASES ONLY)

22De 24De26-Nov 16De 18De 20De

1st dayestimatedlesions forWild Boar B

3 Wildboar shot, 1lame.FMD confirmed4th Jan

Wild boar (B)observedlame (Slivarovo Huntingarea).

Entry of infection to IP1 (Kosti) in period 13-27th based on lesion (assumed 10 days) seenin domestic pig on 7th Jan

FMD Timeline - Kosti and Resovo, Bulgaria.Green boxes are Observed Events (Surveillance findings)

1st Day est CSin wildboarcase (A).

7th Jan: KostiHealing Lesion seen in domestic pig(=IP1). 5 +ve pigs and 1 +ve cow ingroup (FMD-NSP antibody).Blood sampling Kosti Hereford herd (IP3):NSP-ve. Incubating?

EU team visit Resovo. Oldestlesion observed 7-10 daysage.

Earliest infection date (14 day incub.)for Wild boar B

Kosti - Surveillance finds FMD signs 4 days agein Hereford herd (=IP3)

27th December: infection in IP1 musthave occurred on or before this date togive rise to clinical signs/NSP+ves atsampling on 7th Jan.

1st lesions estimated to occur Resovo 8-10th

4th : Turkish buffalo reported in Resovo

SAFETY EVERY DAY

MINISTRY OF AGRICULTURE AND FOOD

BULGARIAN FOOD SAFETY AGENCY* Sofia, 1606, “Pencho Slaveikov” blvd. 15A

( +359 (0) 2 915 98 20, +359 (0) 2 954 95 93, www.babh.government.bg

ADDENDUM

to the dossier submitted to OIE on 5th June 2012 on theFoot-and-Mouth Disease epidemiological situation in Bulgaria

and the application by the Bulgarian Food Safety Agency for recovery of theformer status of the whole territory of Bulgaria as a

“FMD FREE COUNTRY WHERE VACCINATION IS NOT PRACTICED”

20/07/2012

1. IntroductionOn 5th January 2011, Bulgaria notified a case of FMD in a wild boar shot 2 km from theTurkish border in the Region of Burgas. This was the first detection of FMD virus inBulgaria since 1996. Until 7th April, the date of the last outbreak, 11 type O outbreakswere confirmed in livestock in 10-12 km border area which stretches into Turkish Thrace.

Despite of the fact that Bulgaria was FMD free country where vaccination is not practicedfor 16 years and all outbreaks in 2011 were located at a distance of no more than 12 kmfrom the Turkish Border the status of the whole territory of the country (110 994 km2)was suspended and Bulgaria is not currently included among those countries withofficially recognized FMD-free status according to OIE Resolution No 14 (adoptedduring the 80th General Session of the OIE International Committee, May 2012).

On 5th June 2012 Bulgaria submitted to OIE dossier, applying for the recovery of 'FMDfree country where vaccination is not practiced' status.

A preliminary screening of the dossier performed at OIE Headquarters revealed that thedata provided in the application is from the restricted zone only and did not cover thewhole country for which the recovery of status was requested. (Letter by Dr Bernard Vallatof 21st June 2012, Ref.: KHO/KM/SB 30.695).

However the FMD incursion in 2011 was only limited to a small area in the South-EastBulgaria which was clearly proved by the surveillance program implemented successfullyby the Bulgarian Food Safety Agency. The dossier provided by Bulgaria clearly showsthat the spread of FMD was stopped at the border area. All outbreaks were located in the12 km zone along the border with Turkey. The surveillance carried out in the protectionand surveillance zones as well as in the so called restricted area (Cordon Sanitaire) and in

2

certain villages to the north of this area did not detect any evidence of FMDV circulationfor more than 14 months after the last outbreak. The surveillance in domestic livestockhas clearly shown disease absence. The relatively low sero-prevalence and clusteredspatial distribution of sero-positive wild boar and roe deer suggest that the FMD infectionfailed to develop into a large scale epidemic wave in wildlife and faded out within lessthan a year. The FMD incursion was contained by establishing the “Cordon sanitaire”supported by the fact that neither clinical signs nor sero-conversion has been observed inherds of East-Balkan pigs kept on pastures all year just north of this or in other parts ofBulgaria. No clinical signs suggesting FMD have been observed during the regularveterinary activities and clinical surveillance in animal holdings and slaughterhouses onthe whole territory of Bulgaria during April 2011 – July 2012.

As an OIE Member country Bulgaria has always implemented all its obligations to theorganization and the other Member country. Annual reports indicating the FMD statushave been provided regularly. Immediate notifications and follow up reports in case ofoutbreaks of infectious diseases as well as the regular six months and annual reports forthe animal health status of the country have always been regularly submitted. Bulgariawas fare enough to notify to OIE/WAHIS and the world even the only detected FMDVpositive wild boar despite of the very high price that the country had to pay. Since April2011 no FMD outbreaks have been notified through WAHIS because no such outbreakshave been observed on the whole territory of Bulgaria.

Bulgaria considers that fulfills the requirements of articles 8.5.2, 8.5.9 point 1(a), 8.5.45,8.5.47 and 8.5.49 of the OIE Terrestrial Animal Health Code.

In this addendum Bulgaria provides additional information related not only to therestricted zone but to the whole territory of the country for proving its FMD freedom.Short information is provided on the history of the FMD outbreaks in the country in the90’s; summary of the FMD situation in 2011 and 2012; some of the conclusions of theEFSA’s Scientific Opinion on Foot-and-mouth disease in Thrace; the control system inplace for animal health (particularly FMD) and the clinical surveillance of the animalhealth status of FMD susceptible livestock on the whole territory of Bulgaria during 2011- 2012.

2. History of FMD outbreaks in BulgariaIn the 1990’s Bulgaria experienced three FMD type O1 outbreaks, all in the Thraceregion (Figure 1).

The first outbreak (O/BUL/1/91) was reported in July 1991. During that period Bulgariahad already stopped prophylactic vaccination against FMD in the established 30 kmbuffer zone bordering Turkey. The outbreak was restricted to Stefan Karadjovo village(1), Yambol region. Stamping out and ring vaccination was successfully applied as thecontrol policy.

In May 1993, a second FMD outbreak was notified. The disease was detected in a dairycattle holding near the town of Simeonovgrad (2), Haskovo region, located very close to

3

an international transit road. For the second time, stamping out with ring vaccination wassuccessfully applied. In total, 304 infected and contact cattle were killed and buried.

The third outbreak was notified in October 1996 in the village of Malko Sharkovo (3),Yambol region, and stamping out was the control policy used to eradicate the outbreak.No vaccination policy was applied. The hypothesis for the origin of the disease was thatthe virus was introduced with illegally moved susceptible animal(s) from Turkey.

1= Stefan Karadjovo 1991; 2= Simeonovgrad 1993; 3= Malko Sharkovo 1996

Figure 1: Location of FMD outbreaks in Bulgaria in the 90’s

3. Summary of the FMD situation in Bulgaria in 2011 and 2012

On 5 January 2011 the Bulgarian competent authorities reported a confirmed case ofFMD in a wild boar (with lesions indicative of FMD) which had been shot in the Burgasregion about two kilometers north of the Turkish border on 30 December 2011. Isolationand typing of the virus were made by the EU Reference Laboratory (EU-RL).

Measures in accordance with Council Directive 2003/85/EC of 29 September 2003 onCommunity measures for the control of foot-and-mouth disease repealing Directive85/511/EEC and Decisions 89/531/EEC and 91/665/EEC and amending Directive92/46/EEC were implemented from 5 January 2011. Clinical and serological surveillancewas initiated and 11 outbreaks in domestic animals were detected in south-east Bulgariabetween 9 January and 7 April. These were the first outbreaks of FMD in Bulgaria since1996.

All the FMD viruses sampled in Bulgarian Thrace were the serotype O PanAsiaII-Ant10lineage.

4

Certain protection measures against FMD in Bulgaria were introduced on 6 January 2011through Commission Decision 2011/8/EU of 6 January 2011 concerning certain interimprotection measures against foot-and-mouth disease in Bulgaria, which was repealed byCommission Decision 2011/44/EU of 19 January 2011. Protection measures applied until30 September 2011.

Stamping-out and strict surveillance (Cordon sanitaire), but no vaccination was applied toeradicate FMD in domestic animals;

Protection zones and surveillance zones in line with the EU legislation had beenimplemented around each of the 11 FMD outbreaks. The protection and surveillancezones around the second wave of outbreaks (outbreaks 4-11) detected 19 March – 7 April2011 were merged forming a common inner protection zone and outer surveillance zoneuntil all measures in each zone were finalized for all eight outbreaks;

On 18-19 April 2011 clinical examinations were carried out and 2030 samples werecollected from susceptible animal species in the villages inside the protection orsurveillance zones where there were still animals. All samples were negative forantibodies to FMD serotype O.

Sampling and clinical examination were carried out in the villages inside the protectionand surveillance zones on 28-29 April and on 18-19 May 2011. All samples werenegative for antibodies to FMD serotype O.

On 18-19 April and on 18-19 May 2011 clinical examinations and sampling were carriedout in randomly selected villages (40 and 14, respectively) around the surveillance zone,i.e. more than 10 km from an FMD outbreak. Sample numbers for each village were inexcess of the minimum numbers calculated for a 5% detection rate with 95% confidence.All samples were negative for antibodies to FMD serotype O;

On 4 July Order 11-713 lifted the protection and surveillance zones around outbreaks 4-11, implemented the amended restricted zones in Annexes I and II to CommissionImplementing Decision 2011/388/EU of 29 June 2011 amending Decision 2011/44/EUconcerning certain protection measures against foot-and-mouth disease in Bulgaria(valid until 30 September 2011), and reiterated the applicable restrictions.

Surveillance in susceptible wildlife, particularly wild boar was initiated in January 2011.A plan for the eradication of FMD in wildlife, taking into account Part B of Annex XVIIIof Council Directive 2003/85/EC, was submitted to the Commission on 4 April 2011 andapproved by Commission Implementing Decision 2011/493/EU of 5 August 2011approving the plan for the eradication of foot-and-mouth disease in wild animals inBulgaria.

The main components of the Eradication Plan in the Cordon Sanitaire (see the dossiersubmitted on 5th June 2012) were:

- movement restrictions on animals and products;

5

- active surveillance (shooting/trapping, sampling and laboratory analysis) ofsusceptible wild animals;

- documented clinical examination of domestic animal herds in all 106 villagesin the Cordon Sanitaire at least every 21 days;

- at least every 90 days, blood sampling from susceptible animals forserological examination, aimed at detecting a 5% sero-prevalence with 95%confidence in each epidemiological unit (=village).

Under the Eradication Plan sampling of domestic animals in 106 villages in the CordonSanitaire was carried out for serological investigation on 16-25 August 2011 (5203samples), 21-25 November 2011 (5295 samples) and 20-25 February 2012 (5334samples).

A total of 812 wild boar, 68 roe deer, 7 red deer and 2 mouflons were tested for thepresence of FMD virus genome and FMD antibodies between February 2011 and January2012 in the same region. No FMD virus genome could be detected. However, sero-positive animals were found amongst wild boar and roe deer. The sero-prevalence in thewild boar was 6.9 % with a statistically significant difference between adults (9.5%) andjuveniles born in 2011, (1.6 %). Three (4.4%) roe deer were found to be sero-positive.Most of the sero-positive animals were spatially clustered around the FMD outbreaks inlivestock, limited within a radius of 20 km. The relatively low sero-prevalence andclustered spatial distribution of sero-positive wild boar and roe deer suggest that the FMDinfection in wildlife was mainly a short timing disease event, which failed to develop intoa large scale epidemic wave in wildlife and had fade out.

East-Balkan pigs were blood sampled between February 2011 and May 2012 in theregions of Burgas (553 samples), Shumen (558 samples) and Varna (80 samples). Allsamples were negative for antibodies to FMD virus serotype O.

Although the Eradication Plan ended 3 April 2012 all restrictions in the Cordon Sanitaireimposed were still in force till the end of June 2012; The intensive controls onmovements of animals and products, which were implemented during 2011, werefacilitated by a well functioning cooperation between the competent authorities involved.

After the Eradication Plan ended 3 April 2012, surveillance had continued in 37 (locatedin 10 km zone along the border with Turkey) of the 106 villages under a nationalsurveillance plan. Sampling had been carried out in these 37 villages during 28-31 May2012 when 1769 blood samples were collected from susceptible livestock. Samplenumbers for each village were in excess of the minimum numbers calculated for a 5%detection rate with 95% confidence. All samples were negative for antibodies to FMDserotype O;

6

TsarevoMalko Tarnovo

Sredets

Boliarovo

Elhovo

Svilengrad

Topolovgrad

BULGARIA

TURKEY

GREECE Village under surveillance

Figure 2: Villages (n = 37) along the border where clinical and serological surveillancecontinues during April – December 2012.

Intensive monitoring of domestic animals during 14 months after the last outbreak hasshown that the stamping out of the 2011 outbreaks was successful. The results fromwildlife sampled until January 2012 indicate that the spread of FMD virus serotype O,which caused the 2011 outbreak, to wild animals had taken place only in the vicinity ofthe outbreaks in domestic animals and that the spread of the virus to juvenile wild boar(born in 2011) had been very limited and died out before mid summer of 2011.

4. Scientific Opinion on foot-and-mouth disease in Thrace.EFSA Journal 2012;10(4): 2635. 91 pp. doi:10.2903/j.efsa.2012.2635;

Following a request from the European Commission and the Risk Assessment Centrewithin the structure of the BFSA, the Panel on Animal health and Welfare at theEuropean Food Safety Authority (EFSA) was asked to deliver a scientific opinion onFMD in Thrace.

A Scientific Opinion by EFSA on Foot-and-mouth disease in this geographical region(Thrace) was published on the EFSA website on 4 April 2012 together with an ExternalScientific Report on the epidemiology of FMD virus in wildlife in Thrace(http://www.efsa.europa.eu/en/publications/efsajournal.htm).

EFSA concluded that:- Most likely the disease died out in mid-summer 2011, and the areas subject to

intensive surveillance both in Bulgaria and Turkey are now free from FMD inwildlife (and domestic animals). This is based on the observations with

http://www.efsa.europa.eu/en/publications/efsajournal.htm

7

epidemiological considerations (hot summer in 2011, which was detrimentalfor environmental survival of FMDV, a relatively low density of wild boar of~2-3 heads km, absence of clinical signs or virus detections in a considerablylarge proportion of animals inspected and tested in Bulgaria in October-December 2011 exactly in the former area of the infection, as well as furtheraway in Turkey).

- The epidemiological model indicates that the presence of deer in thepopulated area does not alter the spatio-temporal dynamics of the infection inthe model and that deer alone are not able to facilitate spread of the infectionthrough the whole landscape.

- The epidemiological model indicates that the strong temperature dependenceof FMDV survival in the environment explains the seasonal increased chanceof virus fade-out.

- The epidemiological model indicates that continued maintenance (e.g. withmoderately virulent CSFV in wild boar) cannot be expected from a wild boar+ deer host system alone for FMDV. There is need for cross-transmissionbetween wildlife sub-populations due to human movement or cross-transmission to the domestic sector for virus circulation to be maintained.

5. Control system in place for animal health (particularly FMD) in Bulgaria

Bulgaria is divided into 28 regions. The regions are administrative units executing theregional policy of central government bodies. The regional governors are appointed bythe Council of Ministers. Local government is organised through 264 municipalities. Atregional level BFSA has 28 Regional Food Safety Directorates (RFSD) responsible forthe implementation of the official controls (RFSA in each administrative unit of thecountry). All FMD outbreaks in 2011 were detected only in the region of Burgas (02).

Competent authorities

8

The central competent authority for animal health is the BFSA. The BFSA issubordinated to the Ministry of Agriculture and Food (MAF). The Animal Health andWelfare Directorate (AHWD) of the BFSA takes part in the harmonization and draftingof legal implementing documents relevant to its competencies. It also arranges forveterinary preventive measures against infectious and parasitic diseases including FMDand prepares the annual prophylactic, monitoring and eradication programmes. It is alsoresponsible for the preparation of contingency plans, guidelines and instructions for theRFSD on the implementing of animal health measures. The total number of staffinvolved in the animal health sector in the official services in Bulgaria is: BFSA, 28RFSD, 160 veterinary auxiliaries, 265 OMV (official municipal veterinarians).

Furthermore, 1,025 authorised private veterinarians (APV) perform official tasks underthe animal health programmes.

Holding registration, animal identification and movement controls

9

Animal holdings keeping cattle, pigs, sheep and goats are registered on the CentralDatabase (CDB) named VetIS and a unique holding number is allocated to each holding.The CDB is an Internet based system designed for multi-species functionality. TheAHWD is responsible for the management of the information in the CDB. In the CDBdata is entered by 1,400 users, including private veterinarians, veterinarians performingofficial controls on holdings, slaughterhouses, collection centers and others. The nationalsystem of identification and registration of animals requires identification of all speciesby an individual number. The APV are responsible for tagging and registering theanimals and providing monthly data to the OMVs who enter it into the CDB. A newmodule of the CDB was developed and entered into force.

The system for animal movement control is based on animal health movement certificatesissued by the APV responsible for the farm. Passports are issued for large ruminants andequines. All holdings are required to keep holding registers and each change to the herd isentered in a record book.

Animal health controlsControl measures (sampling, testing, and vaccination) prepared by the AHWD andapproved by the MAF, are implemented by the RFSD, OMV and APV. There is ananimal health department in each of the 28 RFSD. At local/district level, there are 265municipalities each supported by an OMV who supervises the performance of the APV.A total of 1,025 APV carry out prophylactic and monitoring of the animal healthprogrammes.

The APV report the results of their work on a monthly basis to the relevant OMV whosummarises the input from all APVs and report to the RFSD. The RFSD reports to theAHWD on the implementation of the animal health programmes in their region on amonthly basis.

Specific eradication and monitoring programmes are approved for different animaldiseases including FMD.

(The Control and coordination Directorate-CCD performs audits at all administrativelevels (AHWD, RFSD, OMV and APV).

Control in slaughterhousesThe total number of RFSD inspectors carrying out the veterinary public health controls(including inspection in slaughterhouses) is 617, of whom 495 are official veterinarians.

Contingency plansUpdated of contingency plans for emergency diseases in Republic of Bulgaria areavailable on:http://babh.government.bg/bg/actualno-department-health&article_id=108.html

For practical purposes the BFSA has based its overall contingency plan on three majortypes:· A Strategy and Resource plan,

http://babh/

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· Operational manuals – FMD + others· Instructions for practical matters during outbreak management.

The contingency plan for emergency diseases in Bulgaria (the strategy and resource plan,the operation manuals of the animal diseases and the practical instructions) is prepared inaccordance with the requirements of national and European legislation, the OIE standardsand characteristics of different diseases.

National reference laboratory (NRL) for FMDThe National Diagnostic and Research Veterinary Institute (NDRVI) is a specializedunit within the the BFSA on diagnostics and research activities in the field of animalhealth. It includes the national reference laboratory (NRL) on FMD which operatesunder the coordination of the Laboratory Activities Directorate of the BFSA.

The NDRVI is accredited to the standards of International Organisation forStandardisation (ISO) 17025:2005 by the Bulgarian Accreditation Service. The separatelocation of the FMD NRL is specified in the current accreditation certificate, which isvalid until 30 June 2013.

The NRL for FMD has a laboratory contingency plan for FMD comprising those methodswhich would be available for detection of FMD antibodies or antigen and comply withthe requirements of the OIE diagnostic manual.

During the FMD epidemics the following tests were used:- serological tests used for surveillance are FMD ELISA Type O and/or FMD

NSP ELISA and LPB ELISA serotype O- Virological tests used for FMD detection and surveillance are RT PCR and/or

Ag ELISA

Staff responsible for FMD testing is well trained for the range of methods applicable andis well aware of the advantages and limitations of each method;

Samples are under the official control during the transportation to the FMD laboratory.Maximum sample turnaround times had been satisfactorily short (1-3 days) also duringthe peak of the 2011 outbreaks;

Whilst in the beginning of the 2011 FMD outbreaks serological samples were analysedusing NSP-ELISA and LPBE (albeit using only reagents for serotype O), towards the endof the outbreaks and for the subsequent serological surveys under the Eradication Planthe vast majority of serum samples were screened only with the type O ELISA. Screeningpositive samples were re-tested with the same method or/and tested with NSP-ELISAcombined with appropriate clinical and epidemiological investigations of the reactoranimal at hand and appropriate clinical, epidemiological and serological, investigations ofthe of susceptible animals of the same epidemiological unit and of susceptible animal thathave been in contact or otherwise epidemiologically associated with the reactor animal. If

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the follow-up investigations provide no evidence for FMDV infection the reactor animalis only then classified as FMD negative.

6. Clinical surveillance of the animal health status of FMD susceptible animals onthe whole territory of Bulgaria during 2011 -2012

The clinical and serological surveillance for FMD carried out in the area borderingTurkey as well as in certain villages to the north and west of this area is described in thedossier submitted to OIE on 5th July 2012

In addition clinical examinations of the FMD susceptible livestock have been carried outin 2011 and 2012 on the whole territory of Bulgaria in the framework of different animalhealth programs without detecting any evidence for FMD:

a) Clinical examinations of the health status of bovines during the implementationof the control programs for bovine tuberculosis, bovine brucellosis & enzooticbovine leucosis in 2011 and 2012 (till end of June)

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD havebeen detected in bovines during clinical examinations of their health status in 2011 and2012 (as by end of June) on the whole territory of Bulgaria in the framework of thecontrol programs for bovine tuberculosis, bovine brucellosis & enzootic bovine leucosis(EBL) (tables 1 and 2)

Table 1: Clinical examinations of bovines in 2011 without detecting any evidence forFMD

Total number ofexisting bovines

Number of clinicalexaminations in theframework of controlprogram for bovinetuberculosis

Number of clinicalexaminations in theframework of the

control program forbovine brucellosis


control program forEBL

Region

herds animals herds animals herds animals herds animals1 Blagoevgrad 6 540 29 133 6 540 27 305 6 540 18 325 39 1 1232 Burgas 4 349 27 087 4 348 23 560 4 349 15 794 105 4 0373 Varna 848 10 989 848 10 921 848 7 258 172 1 9254 Veliko Tarnovo 2 440 21 281 2 440 20 461 2 440 12 916 2 440 5 7505 Vidin 782 7 241 782 7 241 782 5 054 50 2 2346 Vratsa 5 218 17 672 5 218 16 268 5 218 12 428 65 3 1837 Gabrovo 978 8 598 978 8 422 978 4 716 41 2 0648 Dobrich 1 460 22 903 1 460 20 690 1 460 12 138 171 6 2949 Kardjali 13 849 48 915 13 849 44 939 13 849 28 845 38 1 162

10 Kjustendil 1 539 6 296 1 539 6 296 1 539 4 423 18 43411 Lovech 1 283 11 869 1 283 10 151 1 283 6 567 39 2 03412 Montana 1 601 16 027 1 601 15 379 1 601 10 167 92 4 55413 Pazardjik 6 413 19 602 6 413 17 925 6 413 10 042 172 4 59514 Pernik 1 399 6 085 1 399 5 411 1 399 3 975 11 86115 Pleven 4 068 24 443 4 068 18 854 4 068 12 855 111 5 797

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16 Plovdiv 6 321 63 498 6 321 57 387 6 321 36 578 6 321 18 26817 Razgrad 2 820 18 435 2 820 19 433 2 820 13 766 2 820 3 53818 Russe 1 528 14 876 1 528 12 111 1 528 9 088 1 528 3 32519 Silistra 3 072 12 508 3 072 11 879 3 072 5 224 142 1 88420 Sliven 381 27 616 381 24 335 381 16 792 173 8 96921 Smolyan 8 165 15 222 8 165 12 585 8 165 8 572 204 69422 Sofia town 376 4 919 376 4 662 376 2 849 376 1 82323 Sofia-district 5 600 13 533 5 600 13 533 5 600 9 918 55 2 81224 Stara Zagora 2 950 29 272 2 950 28 553 2 950 20 638 195 4 85525 Targovishte 6 366 25 135 6 366 24 433 6 366 15 677 85 3 87026 Haskovo 7 230 35 924 7 230 33 482 7 230 20 702 125 2 74727 Shumen 4 544 24 225 4 544 24 225 4 544 16 108 176 6 42828 Yambol 1 263 23 130 1 263 16 985 1 263 10 091 130 3 412

Total 103 383 586 434 103 382 537 426 103 383 351 506 15 894 108 672

Table 2: Clinical examinations of bovines in 2012 (by end of June) without detecting anyevidence for FMD

Total number ofexisting bovines

Number of clinicalexaminations in theframework of controlprogram for bovinetuberculosis


control program forbovine brucellosis


control program forEBL

Region

herds animals herds animals herds animals herds animals1 Blagoevgrad 6 540 29 133 4 905 21 850 4 905 20 393 39 9282 Burgas 4 854 27 087 3 641 20 315 3 641 18 961 105 3 8503 Varna 1 162 10 989 872 8 242 872 7 692 172 1 7254 Veliko Tarnovo 2 440 21 281 1 830 15 961 1 830 14 897 2 440 3 7505 Vidin 782 7 088 587 5 316 587 4 962 50 2 0346 Vratsa 5 218 17 672 3 914 13 254 3 914 12 370 65 1 1837 Gabrovo 956 7 700 717 5 775 717 5 390 41 1 2648 Dobrich 1 460 22 903 1 095 17 177 1 095 16 032 171 5 4929 Kardjali 13 849 48 915 10 387 36 686 10 387 34 241 38 1 16210 Kjustendil 1 539 6 296 1 154 4 722 1 154 4 407 18 23411 Lovech 1 425 10 582 1 069 7 937 1 069 7 407 39 1 93412 Montana 1 558 15 691 1 169 11 768 1 169 10 984 92 2 45413 Pazardjik 6 413 19 602 4 810 14 702 4 810 13 721 172 3 39514 Pernik 1 399 5 876 1 049 4 407 1 049 4 113 11 76115 Pleven 4 068 24 443 3 051 18 332 3 051 17 110 111 4 79716 Plovdiv 6 321 63 498 4 741 47 624 4 741 44 449 6 321 17 26817 Razgrad 2 820 18 435 2 115 13 826 2 115 12 905 2 820 2 83818 Russe 1 528 14 876 1 146 11 157 1 146 10 413 1 528 1 52319 Silistra 3 072 12 508 2 304 9 381 2 304 8 756 142 1 48820 Sliven 1 981 30 326 1 486 22 745 1 486 21 228 173 7 69921 Smolyan 8 874 15 254 6 656 11 441 6 656 10 678 204 60422 Sofia town 395 4 121 296 3 091 296 2 885 376 1 38323 Sofia-district 5 963 16 822 4 472 12 617 4 472 11 775 55 2 07524 Stara Zagora 2 950 29 272 2 213 21 954 2 213 20 490 195 2 65525 Targovishte 6 366 25 135 4 775 18 851 4 775 17 595 85 1 570

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26 Haskovo 7 230 35 924 5 423 26 943 5 423 25 147 125 2 04727 Shumen 4 544 24 225 3 408 18 169 3 408 16 958 176 5 92828 Yambol 1 263 23 130 947 17 348 947 16 191 130 2 712

Total 106 970 588 784 80 232 441 591 80 232 412 150 15 894 84 753

b) Clinical examinations for the health status of sheep and goat in the framework ofthe program for control of Brucellosis.

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD havebeen detected in sheep and goats during clinical examinations of their health status in2011 and 2012 (as by end of June) on the whole territory of Bulgaria in the framework ofthe control program Brucellosis (tables 3 and 4).

Table 3: Clinical examinations of sheep and goats in 2011 without detecting anyevidence for FMD

Total number of existingsheep and goats

Number of clinicalexaminations in the

framework of the controlprogram for brucellosis in

sheep and goats

Region

herds animals herds animals1 Blagoevgrad 11 399 147 012 11399 90 7382 Burgas 9 503 170 509 9503 22 2663 Varna 11 397 78 908 11397 15 8664 Veliko Tarnovo 11 414 88 745 11414 15 3015 Vidin 9 361 43 398 9361 14 1306 Vratsa 11 339 88 100 11339 14 5597 Gabrovo 3 936 25 625 3936 4 8678 Dobrich 8 478 98 622 8478 16 9569 Kardjali 1 757 85 150 1757 28 959

10 Kjustendil 5 406 35 179 5406 12 90111 Lovech 6 177 58 578 6177 7 70312 Montana 14 551 79 511 14551 23 45613 Pazardjik 5 565 62 469 5565 10 33614 Pernik 3 224 27 609 3224 4 36815 Pleven 13 179 85 669 13179 10 45716 Plovdiv 5 021 140 683 5021 23 38317 Razgrad 6 199 47 788 6199 11 39618 Russe 6 502 51 724 6502 12 81819 Silistra 8 464 65 066 8464 13 94420 Sliven 5 518 90 847 5518 28 81121 Smolyan 3 430 31 260 3430 14 04122 Sofia town 350 13 432 350 1 39523 Sofia-district 7 931 84 680 7931 22 88224 Stara Zagora 8 990 61 218 8990 42 67125 Targovishte 10 632 95 178 10632 19 80426 Haskovo 5 699 111 544 5699 38 856

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27 Shumen 10 496 98 673 10496 28 28928 Yambol 7 626 80 081 7626 26 495

Total 213 544 2 147 258 213 544 577 648

Table 4: Clinical examinations of sheep and goats in 2012 (by end of June) withoutdetecting any evidence for FMD

Total number of existingsheep and goats

Number of clinicalexaminations in the

framework of the controlprogram for brucellosis in

sheep and goatsRegion

herds animals herds animals1 Blagoevgrad 10 952 143 699 7 666 58 9802 Burgas 8 857 202 110 6 200 14 4733 Varna 10 869 71 650 7 608 10 3134 Veliko Tarnovo 10 461 82 087 7 323 9 9465 Vidin 9 217 36 522 6 452 9 1856 Vratsa 10 951 85 466 7 666 9 4637 Gabrovo 3 625 25 100 2 538 3 1648 Dobrich 7 584 94 825 5 309 11 0219 Kardjali 1 731 86 654 1 212 18 823

10 Kjustendil 5 206 32 132 3 644 8 38611 Lovech 5 753 49 504 4 027 5 00712 Montana 14 243 69 543 9 970 15 24613 Pazardjik 5 524 62 351 3 867 6 71814 Pernik 2 595 25 884 1 817 2 83915 Pleven 12 076 80 957 8 453 6 79716 Plovdiv 3 952 110 298 2 766 15 19917 Razgrad 6 314 48 481 4 420 7 40718 Russe 6 367 50 474 4 457 8 33219 Silistra 7 881 65 206 5 517 9 06420 Sliven 4 928 86 137 3 450 18 72721 Smolyan 3 501 33 355 2 451 9 12722 Sofia town 326 9 696 228 90723 Sofia-district 7 989 74 658 5 592 14 87324 Stara Zagora 8 567 59 885 5 997 27 73625 Targovishte 10 632 95 178 7 442 12 87326 Haskovo 5 875 114 106 4 113 25 25627 Shumen 10 423 101 442 7 296 18 38828 Yambol 7 264 107 307 5 085 17 222

Total 203 663 2 104 707 142 566 375 472

c) Clinical examinations of the health status of pigs in the framework of theClassical swine fever (CSF) control program

Out of the FMD outbreaks in Jan – Apr 2011 in Burgas region, no evidence of FMD havebeen detected in pigs during clinical examinations of their health status in 2011 and 2012

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(as by end of June) on the whole territory of Bulgaria in the framework of the controlprogram CSF (tables 5 and 6).

Table 5: Clinical examinations of pig herds in 2011 without detecting any evidence forFMD

Total number ofexisting pigsRegion (1)

herds animals

Number ofclinicallyexamined

herds

1 Blagoevgrad 3 213 7 480 3 2132 Burgas 835 36 130 1293 Varna 381 23 385 754 Veliko Tarnovo 1 843 31 420 6565 Vidin 3 901 6 727 3 8966 Vratsa 2 801 4 322 5797 Gabrovo 811 4 913 7588 Dobrich 1 650 16 997 1 1449 Kardjali 11 378 4

10 Kjustendil 3 014 5 175 2 99611 Lovech 618 5 805 48612 Montana 1 684 9 035 1 36613 Pazardjik 979 24 029 97914 Pernik 911 1 440 115 Pleven 1 350 2 839 2 97616 Plovdiv 596 5 124 55617 Razgrad 36 37 117 2118 Russe 166 93 085 11819 Silistra 1 102 24 530 96120 Sliven 1 791 12 468 4021 Smolyan 72 469 1822 Sofia town 297 1 370 22923 Sofia-district 2 273 9 263 2 27324 Stara Zagora 5 069 83 863 5 06925 Targovishte 235 14 981 7126 Haskovo 2 944 6 382 1427 Shumen 904 57 601 90428 Yambol 2 757 30 425 7

Total 42 244 556 753 29 539

Table 6: Clinical examinations of pig herds in 2012 (by end of June) without detectingany evidence for FMD

Total number ofexisting pigsRegion (1)

herds animals

Number ofclinicallyexamined

herds

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1 Blagoevgrad 3 100 7 217 2 1142 Burgas 835 36 130 1943 Varna 381 23 385 2384 Veliko Tarnovo 1 843 31 420 2185 Vidin 3 901 6 727 2 7206 Vratsa 2 801 4 322 3397 Gabrovo 728 3 696 4628 Dobrich 991 4 640 4129 Kardjali 11 378 9

10 Kjustendil 3 014 5 175 3 01411 Lovech 618 5 805 38512 Montana 1 684 9 035 1 68413 Pazardjik 160 19 879 814 Pernik 911 1 440 34515 Pleven 1 350 2 839 84916 Plovdiv 596 5 124 25017 Razgrad 36 37 117 1018 Russe 31 86 644 1819 Silistra 852 12 332 1620 Sliven 1 791 12 468 2521 Smolyan 16 131 922 Sofia town 143 1 074 16523 Sofia-district 2 110 10 702 93824 Stara Zagora 3 613 92 853 52525 Targovishte 235 14 981 23526 Haskovo 2 755 6 820 11627 Shumen 770 58 947 77028 Yambol 2 757 30 425 2 111

Total 38 033 531 706 18 179

d) Clinical examinations of FMD susceptible livestock before issuing veterinarycertificate in case of animal movements

No evidence of FMD have been detected in susceptible livestock during clinicalexaminations of the health status of the animals before issuing veterinary healthcertificate in case of animal movements on the whole territory of Bulgaria in 2011 and2012 (as by end of June) (tables 7 and 8).

Table 7: Number (n) of FMD susceptible livestock inspected before issuing veterinaryhealth certificate in case of animal movements in 2011

Region Cattle (n) Sheep & goats (n) Swine (n)1 Blagoevgrad 9 624 17 884 6592 Burgas 2 134 10 747 98 6693 Varna 1 539 4 888 108 5724 Veliko Tarnovo 5 482 20 872 41 1205 Vidin 1 738 2 735 5 4716 Vratsa 2 576 5 604 4377 Gabrovo 1 995 7 172 4 648

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8 Dobrich 5 561 13 515 27 1689 Kardjali 1 115 2 190 0

10 Kjustendil 727 10 154 5311 Lovech 5 473 16 649 10 37612 Montana 3 898 5 226 3 95613 Pazardjik 1 866 6 732 39 43614 Pernik 597 742 815 Pleven 4 182 10 013 4 26016 Plovdiv 16 906 46 867 6 12117 Razgrad 3 105 12 187 53 10918 Ruse 2 820 4 968 210 39619 Silistra 1 689 21 179 11 57620 Sliven 5 486 12 697 13 60421 Smolyan 871 7 203 2922 Sofia town 2 299 8 205 8 23223 Sofia District 873 5 967 34824 Stara Zagora 7 135 12 509 144 16425 Targovishte 6 212 30 789 26 95526 Haskovo 3 462 12 492 3 26927 Shumen 3 245 9 363 115 89828 Yambol 2 935 16 519 39 850

Total 104 430 336 068 978 384

Table 8: Number (n) of FMD susceptible livestock inspected before issuing veterinaryhealth certificate in case of animal movements during the period 1st Jan – 30th June 2012

Region Cattle (n) Sheep & goats (n) Swine (n)1 Blagoevgrad 4 470 15 029 3512 Burgas 1 772 20 914 51 6903 Varna 1 164 4 187 58 4004 Veliko Tarnovo 2 285 7 768 18 9965 Vidin 965 1 367 2 3086 Vratsa 1 732 5 335 147 Gabrovo 933 3 268 2 1108 Dobrich 2 967 8 971 12 6159 Kardjali 1 897 8 198 0

10 Kjustendil 913 2 263 1511 Lovech 2 239 7 016 5 54912 Montana 1 948 2 399 89813 Pazardjik 1 493 3 211 23 40914 Pernik 415 645 015 Pleven 2 810 7 602 84416 Plovdiv 10 704 22 940 3 43617 Razgrad 2 227 13 810 20 40718 Ruse 2 098 4 372 83 79919 Silistra 1 132 17 819 6 48020 Sliven 4 949 15 838 5 11621 Smolyan 383 3 544 022 Sofia town 1 062 3 477 6 25523 Sofia District 890 2 254 14024 Stara Zagora 3 668 7 179 55 40025 Targovishte 3 282 29 647 12 115

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26 Haskovo 2 942 10 379 1 77227 Shumen 2 981 13 789 60 64128 Yambol 2 122 7 985 16 424

Total 66 443 248 943 449 184

e) Clinical examinations of FMD susceptible livestock at slaughterhouses in 2011and 2012

No clinical signs or lesions suggesting FMD have been observed at slaughterhouses onthe whole territory of Bulgaria during 2011 and 2012 (tables 9 and 10).

Table 9: Number (n) of FMD susceptible livestock inspected in slaughterhouses inBulgaria in 2011

Region Cattle (n) Sheep & goats (n) Swine (n)1 Blagoevgrad 575 567 69532 Burgas 0 2497 653 Varna 970 2980 243094 Veliko Tarnovo 84 277228 390825 Vidin 0 132 26646 Vratsa 1216 1083 13537 Gabrovo 3980 1063 211908 Dobrich 512 393 155779 Kardjali 3143 10069 13252

10 Kjustendil 164 717 492811 Lovech 866 954 953712 Montana 492 372 93713 Pazardjik 247 1289 4705114 Pernik 0 0 015 Pleven 219 416 6587916 Plovdiv 6736 9806 14032817 Razgrad 0 0 2018 Ruse 153 0 3911219 Silistra 750 9043 3231120 Sliven 1921 3375 3575521 Smolyan 0 0 022 Sofia town 0 6814 228623 Sofia District 0 8 1924424 Stara Zagora 53 299 1137725 Targovishte 1620 3634 11552626 Haskovo 60 1279 2435027 Shumen 325 2358 2055728 Yambol 0 4255 13086

Total 24086 340631 706729

Note: The table includes data for inspected and slaughtered in slaughterhouses large andsmall ruminants and swine originating form all 28 regions of Bulgaria, as well as ofanimals imported for slaughter from EU countries. For each region is indicated thenumber of animals slaughtered in slaughterhouse located in the region – the inspectedanimals can originate from other regions of the country or EU.

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Table 10: Number (n) of FMD susceptible livestock inspected in slaughterhouses inBulgaria during 1st Jan – 30th June 2012

Region Cattle (n) Sheep & goats (n) Swine (n)1 Blagoevgrad 478 3509 40662 Burgas 0 0 03 Varna 410 1759 138024 Veliko Tarnovo 0 69599 112985 Vidin 0 0 17886 Vratsa 671 652 2277 Gabrovo 2134 2321 98388 Dobrich 333 3776 17769 Kardjali 1791 3662 8389

10 Kjustendil 121 1153 378811 Lovech 373 944 587812 Montana 236 229 26513 Pazardjik 146 1772 2984814 Pernik 0 0 015 Pleven 143 457 4210116 Plovdiv 3044 6255 6025617 Razgrad 0 0 018 Ruse 101 35 2407119 Silistra 254 2311 1862820 Sliven 929 2797 2345421 Smolyan 0 0 022 Sofia town 0 15443 63323 Sofia District 0 42 894824 Stara Zagora 34 70 563225 Targovishte 711 3047 6296426 Haskovo 45 2014 1161427 Shumen 120 135 552828 Yambol 0 3638 7435

Total 12074 125620 362227

Note: The table includes data for inspected and slaughtered in slaughterhouses large andsmall ruminants and swine originating form all 28 regions of Bulgaria, as well as ofanimals imported for slaughter from EU countries. For each region is indicated thenumber of animals slaughtered in slaughterhouse located in the region – the inspectedanimals can originate from other regions of the country or EU.

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