AMERICAN COLLEGE OF INTEGRATIVE MEDICINE AND DENTISTRY

PRESENTS

OXYGEN/OZONE THERAPY IN

DENTISTRY

FUNDAMENTAL SCIENTFIC FACTS ABOUT OXYGEN/OZONE

UNIVERSITY OF HAVANA, CUBA DEPARTMENT OF PHARMACOLOGY PROF. OLGA SONIA LEON FERNANDEZ VIENNA, OCT. 8 -10, 2011

*DISINFECTION PROPERITES*NON-TOXIC WOUND CLEANING

*IMPROVED AND ACCERATED WOUND HEALING

FUNDAMENTAL SCIENTFIC FACTS ABOUT OXYGEN/OZONE

UNIVERSITY OF HAVANA, CUBA DEPARTMENT OF PHARMACOLOGY PROF. OLGA SONIA LEON FERNANDEZ VIENNA, OCT. 8 -10, 2011

+ACTIVATION OF RED BLOOD CELL METABOLISM WITH IMPROVED OXYGEN RELEASE

+ACTIVATION OF ENZYMATIC ANTIOXIDANTS AND RADICAL SCAVENGERS

FUNDAMENTAL SCIENTFIC FACTS ABOUT OXYGEN/OZONE

*ACTIVATION OF IMMUNOCOMPETENT CELLS AND RELEASE OF CYTOKINES SUCH AS

INTERFERONES AND INTERLEUKINS. MODULATION OF THE IMMUNE SYSTEM

UNIVERSITY OF HAVANA, CUBA DEPARTMENT OF PHARMACOLOGY PROF. OLGA SONIA LEON FERNANDEZ VIENNA, OCT. 8 -10, 2011

FUNDAMENTAL SCIENTFIC FACTS ABOUT OXYGEN/OZONE

UNIVERSITY OF HAVANA, CUBA DEPARTMENT OF PHARMACOLOGY PROF. OLGA SONIA LEON FERNANDEZ VIENNA, OCT. 8 -10, 2011

*INCREASED ANTIOXIDANT CAPACITY THROUGH ACTIVATION OF CELLULAR

ANTIOXIDANT SYSTEMSCATALASE, SUPEROXIDE DISMUTATSE

CATALASE

FUNDAMENTAL SCIENTFIC FACTS ABOUT OXYGEN/OZONE

*ANTIINFLAMMATORY EFFECT*INCREASED CIRCULATION VIA NITRIC OXIDE

*HIGH REDOX POTENTIAL

WHAT IS

OZONE ?

• A chemical compound consisting of three oxygen atoms (O3).

• The elemental form of oxygen occurring naturally as a result of ultraviolet energy or lightning causing a temporary recombination of oxygen atoms into groups of three.

• The form of oxygen that is formed by an electric discharge field of a corona discharge generator (simulates lightning).

• Oxygen is the only gas that can accept and carry an

electrical charge. Remember, the terminal electron acceptor.

HOW IS OZONE CREATED?

OXYGEN AND A ENERGY SOURCE

A MEDICAL GRADE OXYGEN FED COLD CORONA DISCHARGE OZONE UNIT

AND SEARCH “OZONE DENTISTRY”PubMedUS National Library of Medicine National Institutes of Health Search term Search database All DatabasesPubMedProteinNucleotideGSSESTStructureGenomeBioProjectBioSampleBioSystemsBooksConserved DomainsClonedbGaPdbVarEpigenomicsGeneGEO DataSetsGEO ProfilesHomoloGeneMeSHNCBI Web SiteNLM CatalogOMIAOMIMPMCPopSetProbeProtein ClustersPubChem BioAssayPubChem CompoundPubChem SubstancePubMed

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1. Treatment of Root Canal Biofilms of Enterococcus faecalis with Ozone Gas and Passive Ultrasound Activation. Case PD, Bird PS, Kahler WA, George R, Walsh LJ. J Endod. 2012 Apr;38(4):523-6. Epub 2012 Feb 2. PMID: 22414842 [PubMed - in process] Related citations

2. Ozone therapy in dentistry: A strategic review. Saini R. J Nat Sci Biol Med. 2011 Jul;2(2):151-3. PMID: 22346227 [PubMed - in process] Free PMC Article

191 articles

PRIMARY ISSUES IN DENTISTRY

• TRAUMA

• INFECTION

• BACTERIAL

• FUNGAL

• VIRAL

• PARASITIC

These infections can be:

• Acute• Chronic• Bacterial • Viral• Fungal• Parasitic• OR… A some combination of the above• And RESISTANT to current therapeutics

Integrative Treatment Regimens

• Antibiotics• Antifungals• Antivirals• Antiparasitics• Antiseptics• Essential Oils/Plant Extracts• Homeopathics• Lasers• Ultraviolet Light• Oxygen/Ozone

Therapies

All of these regimens have proven to be effective in the treatment of infections

BUT…

MOST OF THE REGIMENS HAVE SIGNIFICANT SIDE EFFECTS

EXCEPT…

OXYYGEN/OZONE THERAPY

Multitude of resources for use in human treatment.

When used at a therapeutic level

DOSE AND LANGUAGE USE FOR OZONE THERAPY IN DENTISTRY

• EXPRESSED AS MICROGRAMS PER MILLILITERS OF OXYGEN (mcg/ml)

• ALSO EXPRESSED AS “GAMMA”• INTERNAL USE RANGE: 6 - 30µg/ml (dependent on

application)

• EXTERNAL USE RANGE: 40- 50µg/ml (root canal therapy & caries)

• **START with a LOW dose and GO SLOW***

What Treatment Regimens willEffectively Eliminate:

• BACTERIA

• VIRUSES

• FUNGI

• PARASITES

1. Bacteria 2. Ozone contacts cell membrane 3. Ozone (peroxides) penetrates cell membrane 4. Rupture of cell membrane begins 5. Membrane loses integrity 6. Bacteria lyses due to ozone

OZONE IS PRODUCED BY ANTIBODIES DURING BACTERIAL KILLING IN INFLAMMATION

THE SCRIPPS RESEARCH INSTITUTE NOVEMBER 18, 2002

LERNER, WENTWORH ET AL. SCIENCE

WHITE BLOOD CELLS/ PLASMA CELLS/

ANTIBODIES

OXIDATION /BIOFILM

The cover story in “THE JOURNAL OF THE AMERICAN DENTAL ASSOCIATION,” Vol 140, No 8 978-986 is “Periodontitis: An Archetypical Biofilm Disease” It states that “Periodontitis is a classic example of biofilm-mediated diseases.” The article concluded that, “Periodontitis, like other biofilm infections, is refractory to antibiotic agents and host defenses because the causative microbes live in complex communities that persist despite challenges that range from targeted antibiotic agents to phagocytosis.” The clinical implications concluded that “The regular delivery of non targeted anti-biofilm agents may be an effective strategy for treating biofilms, especially if these agents include oxidative agents that dissolve the biofilm matrix.”

OZONE OXIDIZES / DESTROYS BIOFILM

BIOFILM DISEASES

• Periodontal Disease

• Carious Disease

• Endodontic Disease

• Post Extraction

• Implant Issues

Current Therapies in Dentistry

• Caries (tooth decay)

• Infection of head and neck

• Periodontal Disease

• Root Canal Therapy

• Enhanced wound healing (post-surgical)

• Soft tissue lesions

IS THIS A LOCAL OR SYSTEMIC PROBLEM?

BOTH!!!

Pulp Exposure?

Deep Caries?

What’s under sealants?

ARRESTMENT OF CARIES

THE CASE FOR PIT AND FISSUE SEALENTS WITH OXYGEN/OZONE

60 second exposure 46µg/cc

2 minutes post exposure

46 micrograms/cc – 60 second exposure

OZONE

ENDODONTIC REALITY

FUMIGATION PROCEDURE WITH 40 – 50 µg/cc EACH ROOT EXPOSURE TIME 40-60 SEC.

A “Dry Socket”?

Periodontal Problems?

CODE #162

TWO MONTHS OF TREATMENT DR. JOSE DE CASTRO-COIMBRA

History of Ozone

CHRISTIAN FREDRICH SCHONBEIN DISCOVERED OZONE IN 1840

• 1881- John Harvey Kellogg, MD, from Battle Creek, MI used ozone to treat diptheria.

• 1896- Nikola Tesla patented first O3 generator and sold them to doctors for medical use.

• 1898- Benedict Lust, MD, New York- Founder of Naturopathy, wrote about ozone therapy.

1885 Florida Medical AssociationPublished: Ozone

• Written by Charles J. Kenworthy M.D. • Practicing medicine in Jacksonville, Florida. • Proved that ozone was in regular medical usage

in the United States before 1885. • Therefore, it predates the 1906 Pure Food and

Drug Act and the FDA. • Ozone for medical usage is grandfathered in the

United States, and held perfectly legal for use without censure.

Ozone History in Dentistry

• 1932 - E.A. Fisch, a dentist uses ozonated water in dental procedures and pioneered its use in medicine. (1932-1998 rise and fall of antibiotics)

• 1998 - Edward Lynch publishes a series of articles investigating the efficacy of ozone on dental infection.

• 1998 – Mollica, Harris first introduced to oxygen/ozone therapy in dentistry to United States

• 2000 – Harris delivers first comprehensive oxygen/ozone lecture at Capital University of Integrative Medicine.

• 2000 – Oxygen/Ozone becomes curriculum based at CUIM. • 2000 – Oxygen/Ozone Therapy in Dentistry introduced in the

clinical setting at National Integrative Health Associates – CUIM clinic

• 2002 – Mollica, Harris Oxygen-Ozone Therapy “Applications in Biologic Dentistry”. Presented at first continuing education on oxygen/ozone in dentistry

• 2004 – Edward Lynch – chief editor – Ozone: The Revolution in Dentistry

• 2007 – Mollica, Harris Cyto-Immunologic Nutritional Supportive Therapies for Oxygen/Ozone Therapy

• 2008 – International Academy of Oral Medicine and Toxicology accepts Oxygen/Ozone Therapy in Dentistry as scientifically valid.

• 2008 – IAOMT scientifically validates endodontic therapy with oxygen/ozone (G. Cole)

• 2009 – IAOMT publishes Oxygen/Ozone in Dentistry • 2009 – Academy of General Dentistry introduces

subject code 162 – Ozone Therapy

THE SCIENCE

OF OZONE THERAPY

CORONADISCHARGE

Properties of Ozone

In Vitro (Ex Vivo) Powerful Oxidizer–Kills bacteria, fungi and viruses at lower concentrations than chlorine (1 molecule of ozone = 3,000 to 10,000 molecules of chlorine) AND kills 3500 times faster than chlorine.

●Also oxidizes phenolics (poisonous compounds of methanol and benzene), pesticides, detergents, chemical manufacturing wastes and aromatic compounds more rapidly and effectively than chlorine, yet WITHOUT chlorine’s harmful residues (Trihalomethanes, etc.).

Important Aspect to Consider *AGAIN*

Oxygen/Ozone can accept AND

Carry an electrical charge

Biofilm, Bacteria, Virus, Fungi, Parasites, Infection and

Inflammation all are positively charged

Ozone has a negatively

charged area

The In Vivo Therapeutic Oxidative

BURST

0Z0NE

PLASMA

REACTIVE OXYGEN SPECIES

ROS (early phase)

LIPID OXIDATION PRODUCTS

LOPs (late phase)

O3 H2O2

O1

–Ozonated Water: Ozone gas is bubbled through water, creating a solution that has a shelf life of 10 hours at room temperature and 5 days in the refrigerator. Ozonated water is used to treat:

• Wounds• Burns• Intestinal problems• Slow healing skin infections• Gynecological Infections• Also used for colonics• and enemas

– Ozonated Oil (primarily olive oil): Ozone is bubbled through oil until it forms a salve/balm type consistency. This is used to treat:

• Skin conditions, infections with bacteria, fungi and viruses.• Hemorrhoids• Vulvovaginitis• Insect bites/bee stings• Gingivitis• Placed on capsules and ingested to treat ulcers, gastritis and

giradiasis

TMD ApplicationsFOLLOW “Standard of Care Procedures”

IF symptoms fail to resolve

THEN consider ozone treatment due to the probability of an

infection/inflammation in the joint(s) or chronic inflammation of

ligaments, tendons, muscles or ganglia

THE SCIENCE/RESEARCH OF OXYGEN/OZONE ALLOWS FOR…….!!!

• NON-TOXIC SAFE AND EFFECTIVE APPLICATION

• TRUE BIOLOGIC AGENT

• MINIMAL TO NO SIDE EFFECTS

• ECONOMIC THERAPY

IN A BIOLOGIC SENSE…….

• THE CREATION OF A THERAPEUTIC OXYGEN/OXIDATIVE

“BURST” WHICH INDUCES A MULTI-FACTORIAL BIOCHEMICAL/

PHYSIOLOGIC RESPONSE THERE BY REVERSING A STATE OF

ANAEROBIOSIS CONTAINED WITHIN THE AFFLICTED

TISSUES….MOLLICA

OTTO WARBURG

INTEGRATION OF OZONE THERAPYINTO

DAILY BIOLOGIC DENTAL PRACTICE

NASAL INSUFFLATION

+O3 C10H18O3

TRIOZONIDE OF TRIOLEIN

NOT AN OXIDANT

OXIDATION OF OLIVE OIL SO WHAT’S THE POINT?

– Inhalation of Ozonides: An ozone/oxygen mixture is bubbled through olive oil and gaseous compounds are created that are a species of triozonides of triolein (C10H18O3) that are safe to inhale. These triozonides decompose at the aqueous level of the lung alveolus. The thereby create oxidative peroxides that are used to treat:

AllergiesAsthmaOther respiratory diseasesSinus infectionsFoundational procedure

THE CREATION OF AROMATIC TERPENES!

BIOFILMAn aggregate of microbes with a distinct

colonizing architecture.

LUNG ALVEOLUS WITH BIOFILM FORMATION

DYSFUNCTIONAL CILIA DUE TO MICROBIAL TOXINS FROM BIOFILM

EAR INSUFFLATION: THE OXYGEN/OZONE BEING DELIVERED TO THE TYMPANIC

MEMBRANE MUST BE MOISTENED PRIOR TO CONTACT!

MOISTURE CAN BE DELIVERED VIA BUBBLING OXYGEN/OZONE THROUGH THE OZONATED WATER SNAKE OR MOISTENED AT THE CUPS OF THE

STETHOSCOPE WITH WET GAUZE

(use a fan to blow the gas away from the face)

Ear Infections (German Research)

17 patients received 15 sessions of rectal ozone plus ozonized oil

drops for chronic suppurative O.M. 16 tested negative at the end of

two cycles, and 14 improved their hearing loss. Mastoid x-ray

improvement noted in 13. Another study remarked on the

improvement noted using O2/O3 irrigations of the TM.

This is the preferred therapy for recurrent otitis media as opposed to repeated and

prolonged use of antibiotics.

• Vestn Otorinolaringol. 2009;(3):34-5.

• [Treatment of patients with acute and chronic suppurative middle ear diseases using

ozone preparations].

• [Article in Russian]

• Sin'kov EV.

• Abstract

• Methods of clinical application of gaseous ozone and ozonated solutions are described

with special reference to the treatment of patients with acute and chronic suppurative

middle ear diseases. Results of otoscopy, microbiological and cytological studies confirm

therapeutic efficiency of the above techniques as accelerating recovery of patients with

this pathology. It is recommended to include ozonotherapy as an additional method in

the combined treatment of acute and chronic suppurative otitis media.

It is recommended to include ozonotherapy as an additional method in the combined treatment of acute

and chronic suppurative otitis media.

Vestn Otorinolaringol. 1999;(2):48-9. [Reasons for the application of medical ozone in the treatment of chronic purulent mesotympanitis]. [Article in Russian] Shakov VIu, Edeleva AN. Abstract Ozone-oxygen mixture (ozone concentration 600 microg/l) was used in irrigations of the tympanic cavity in 52 patients with chronic purulent mesotympanitis (CPM). The course of the treatment consisted of 5-7 procedures. 18 CPM controls received standard treatment without the irrigations. The course of the ozone-oxygen irrigations produced good results: ear discharge and tympanic mucosa inflammation stopped in 81% of the irrigated patients, levels of myeloperoxidase which marks inflammation reduced significantly, bactericidal effects of the mixture were observed for all the detected pathogens, antibiotic sensitivity of the bacteria rose. Fast antiinflammatory result of ozone therapy was due not only to its bactericidal effect but also via its antihypoxic and immunomodulating mechanisms

Fast antiinflammatory result of ozone therapy was due not only to its bactericidal effect but also via its antihypoxic

and immunomodulating mechanisms

MULTI-TONSILULAR FORMS

TONSIL-FASCIA SPACE INFUSION TECHNIQUE

• PRINCIPLE PART OF COMPREHENSIVE LYMPHATIC THERAPY • PURPOSE: TO DRAIN AND DISENFECT THE TONSILS,

SURROUNDING FASCIA AND SOFT TISSUE(RETRO-PHARYNGEAL SPACE)

• 6 TO 10µg/cc in syringe with 30g 1”needle • Insert needle up to hub through tonsil. • Slowing start infusion at the same time, slowly retract. This

will dispense oxygen/ozone throughout area. • Infusion a total of 1 – 2cc per side • Expectations: 24 – 72 hours drainage and possible outbreak

of pus-like lesions. This is good and will remediate within a few days.

ONE INFUSION 2-3CC AT 6 - 10µG/CC

QUESTIONS??