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8.1 THE ABIOTIC AND BIOTIC COMPONENTS OF THE ENVIRONMENT
The abiotic and biotic components of an ecosystem
Fill in the blank / (FITB) The biotic components consists of
-------------------- : Autotrophs, Example: -------------------
------------------- Heterotrophs Example: --------------------
Secondary consumers Example: --------------------
Tertiary consumers Example: --------------------
Decomposers Example: --------------------
Food Chain , Food webs and Trophic level
Trophic level Functional group Food chain 1 Food chain 2
1
2
3
4
Producers
Primary consumers
Secondary
consumers
Tertiary consumers
Grass
Rats
Snakes
Hawks
Water plants
Tadpoles
Dragonfly
nymphs
Frogs
Pyramid of numbers
Represents the number of individuals at each trophic level of food chain at a
particular time. The number of organisms decreases up the trophic levels.
Hawks
Snakes
Rats
Grass
secondary consumers primary consumers
trophic level 4
trophic level 3
trophic level 2
tertiary consumers
producers trophic level 1
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QUESTION TO ANSWER / QTA 1
Figure 1 shows a terrestrial ecocystem.
(a) Abiotic and biotic components influence the lives of organism. Fill in the boxes in Figure
1 to show two more abiotic components and two more biotic components. [2 marks]
(b) On Figure 2, mark and label one example off the following organisms.
(i) (i) Producer (ii) Consumer (iii) Decomposer [2 marks]
(c) Based on figure 2 , construct one food chain consisting of four trophic level.
1st trophic level2nd trophic level3rd trophic level4 th
trophic level [2 marks]
Figure 1
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(d) An organism in the 3rd trophic level in Figure 1 has decreased in number.
(i) Based on the food chain you have constructed in (c), suggest a way to increase
the numbers of this organism again.
…………………………………………………………………………………….
….………………………………………………………………………………..
[1mark]
(ii) Explain your suggestion in (d)(i)
………………………………………………………………………………………….
………………………………………………………………………………………….………………………………………………………………………………………….
…………………………………………………………………………………………
[2 marks]
( e )The organisms in the trophic level in Figure 1 absorbs 15 000 kJ solar energy. Energy
loss at each trophic level is 90%. Calculate the total every transferred to the organisms
in the 3rd trophic level.
= -------------------------kJ
[2 marks]
Energy Flow
i The producers absorb -------------------energy from the sun and convert it
into ------------------- stored in organic molecules during -------------------.
ii When a carnivore eats a -------------------, the herbivore’s available energy
is transferred on to the carnivore. The carnivore also loses energy
through respiration, ------------------- and -------------------.
iii About -------------------of the energy lost to the environment, only -------------
------ is converted into new tissue and storage material (bio mass).
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QTA 2
Figure 2 represents the energy flow through a food chain consisting of four organisms.
The total energy in the tissue of the green plant is shown in the box.
Figure 2
(a) Complete the other boxes to show the total energy in the tissues of each of
the consumer, assuming 90% loss of energy at each of the stage in the
food chain.
[1 mark]
(b) State one process is likely to be responsible for the loses of energy from
one stage to another
…..………………………………………………………………………………………
………………………………………………………………………………………….. [1 mark]
(c)(i) What is meant by the term producer in the food chain?
…….……………………………………………………………………………………
…..……………………………………………………………………………………..
[2 marks]
(ii) State the source from which the producer obtains its energy
………..……………………………………………………………………………….. [1 mark]
(d) Explain why there are rarely more than four or five trophic levels in a food chain.
…………………..……………………………………………………………………….
………………..………………………………………………………………………….
…………………….……………………………………………………………………..
[2 marks]
Greenplant
Primaryconsumer
Tertiaryconsumer
Secondaryconsumer
500 kJ
90%energy
loss
90%energy
loss
90%energy
loss
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(e) Paddy caterpillar sparrow eagle
Based on the above food chain, explain how the practice of spraying crops with
insecticides, for example DDT can lead the predator being harmed.
…………………………………………………………………………………………..
…………..………………………………………………………………………………
………………………………………………………………………………………….
………………………..……………………………………………………………………
[2 marks]
(f) Construct a food web involving the following organisms found in a freshwater pond.
Start with producers at the bottom of your web.
[3 marks]
Green algae, large carnivorous fish, water beetle, tadpoles,
water fleas, water snails, small fish, mosquito larvae
Water beetles
T large carnivorous fish
Small fish
Water fleas mosquito larvae water snails
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Interaction between biotic components
(a) Symbiosis
A close interaction between different species which ------------------- -------------------
and ------------------- with each other for an extended period of time.
(i) Commensalisms
One partner benefits (commensal) while the other (-------------------) derives neither
------------------- nor -------------------.
Epiphytes
plants which grow on other plants to obtain ------------------- and support.
example: pigeon orchids, staghorn ferns and bird’s nest ferns.
Epizoics
• animals which live on the body of other animals
• a -------------------gets free transportation and feeds on food scraps left behind by
the -------------------
(ii) Mutualism • A relationship between two species of organisms in which ------------------- .
• Example 1: Association of leguminious plants and -------------------.
Interactions between organisms
------------------- ------------------- Symbiosis
------------------- -------------------
Competition
-----------------
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(iii) Parasitism
One organism ------------------- (parasite) while the other is harmed (---------------).
Types : a. Ectoparasitesb.Endoparasites
(b)Saprophytism
Living organisms obtain food from ------------------- and ------------------- organic matter
Example : Saprophytic bacteria, ------------------- and -------------------
(c )Prey-predator
An organism (prey) is haunted and ------------------- by a stronger and bigger organism
(predator).
------------------- by the predator
------------------- in size compared
to the predator
The prey population is ---------------
than the predator population
The prey relies on speed or
------------------- to avoid being
caught by the predators.
Predators usually have long canine
teeth, ------------------- vision and sharp
claws to capture and kill their prey.
Have hooked ------------------- to tear
the flesh of their prey.
The prey – predator relationship between a rat and an owl.
The rat is the ------------------- of the owl. The owl is the -------------------.
An increase in the ------------------- population followed by an ------------------- in the
------------- population.
When the rat population ------------------- due to increasing predation or other factors
such as the spread of diseases, the owl’s ------------------- ------------------- becomes
limited and this leads to a ------------------- in the population of predators.
The prey –predators regulate each other’s population in a cyclical manner which
maintains the populations of both organisms in a ------------------- -------------------
Prey Predator
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The interactions between biotic components in relation to feeding.
Figure 3
Figure 3 shows a plant species P with green leaves on a tree trunk.
The interaction between species P and the host plant, Q is ------------------- Species P is
a type of ------------------- which is able to synthesize their own food by -------------------
process. It grows on the tree trunk to achieve a greater height so that it can receive more
------------------- for photosynthesis process. It does not absorb nutrients from the host
tree. The host tree neither gains nor loses
How species P obtains their needs and adapted to the environment
• Roots system
Occupied by -------------------.
The ants bring all kinds of rubbish and remnants of food to the roots.
These materials gradually ------------------- into humus.
• Leaf
Arrangement in the form of a ------------------- It can collect falling ------------------- which then decomposes into humus
Corrugated leaves with ------------------- can direct rainwater and dew to the roots
Leaf with thick cuticle to reduce -------------------
P
Q
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QTA 3
Interdependence between organisms is a phenomenon that is always operating between
in a community of organisms.
Figure 4
(a) Name the type of relationship between the mushroom and the decaying branch.
………………………………………………………………….. [ 1 mark ]
(b) What is the benefit gained by the mushroom through this association in (a)?
………………………………………………………………………………………………..
………………………………………………………………………………………………..[ 1 mark ]
( c) State the function of the parts labeled X and Y
X : ….…………………………………………………………………………………………..
Y :…………………………………………………………………………………………….[ 1 mark ]
(d) Give two important contributions of mushrooms to man.
(i) ………………………………………………………………………………………….
(ii) ………………………………………………………………………………………….[ 2 marks]
(e) (i) Suggest one example of an organism that shares the same method of nutrition asin Figure 4.
………………………………………………………… [ 1 marks](e) (i) Explain the role of this organism in the ecosystem
……………………………………………………………………………………………….
………………………………………………………………………………………………… [ 2 marks]
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Competition
An interaction between organisms to get the same resources that is in -----
-------------- supply.
------------------- organisms will migrate or die
Animals compete for shelter, ------------------- and breeding mates.
Plants compete for water, nutrients, light and space.
Types of competition
------------------- competition
* Members of the -------------
species compete for the
same resources.
* Examples:
1. Paramecium --------------
aurelia among
themselves.
2. maize plants among
themselves.
------------------- competition
* Competition between ------
------------- species
* Examples:
1. Paramecium --------------
aurelia and Paramecium
-------------------
2. maize plants and
paddy plants.
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8.2 COLONISATION AND SUCCESSION IN AN ECOSYSTEMEcosystem
TERM MEANING
Species A group of organisms that look alike and have -----------------
-- characteristics, share the same ecological niche and are
capable of inter ------------------- and producing fertile ----------
For example, ------------------- and water lilies are two
different species of water plants.
Population A group of organisms of the ------------------- species living in
the same ------------------- at the same time.
For example, a population of elephants living in a jungle.
Community Several ------------------- of different species living in the
same habitat in an ecosystem.
For example, a freshwater pond community.
Ecosystem A community of ------------------- organisms -------------------
with each other and with the ------------------- environment.
Examples : -------------------, a wetland and a pond
Niche The ------------------- of an organism in the ecosystem.
Organisms that undergo metamorphosis in their life cycles
occupy different niches.
For example, a ------------------- lives entirely in ------------------
and utilizes different resources from those of an adult frog
which lives mainly on -------------------.
Habitat A natural environment where organisms -------------------
Example, a single tree can be a habitat for caterpillars and ------
-------------.
CommunitySpecies
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COLONISATION AND SUCCESSION IN MANGROVE SWAMPQTA 4 : Figure 5 shows the geographical distribution of mangrove trees along the east
coast.
Figure 5
1
(a) (i) Name the mangrove trees in zone A to D.A : ………………………… B : ……………………
C : ………………………… D : …………………… [4 marks]
(ii) Among the mangrove trees stated in (a) (i), which is the pioneer species?
……………………………………………………………………… [2 marks]
(b) State two problems faced by the pioneer species in the colonization of
swampy area.
.………………………………………………………………………………………………..
......................................................................................................................................
[2 marks ]
(c) Explain two adaptive characteristics that enable the pioneer species to survive inthe swamp area.
………………………………………………………………………………………………..…..
………………………………………………………………………………………. ……….. … ………………………………………………………………………………………...................
…………………………………………………………………………………… [2 marks]
(d) (i) What is meant by viviparous seedlings?
……………………………………………………………………………………[ 1 mark ]
(d) (ii) What is the benefit of this to the mangrove plant?
…………………………………………………………………………………………………….
…………………………………………………………………………………… [1 mark]
A
C
BDE
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A. SUCCESSION IN A MANGROVE SWAMP
(B) Colonisation and succession in a pond
Avicennia sp. and ------------------- zone (----------------
species )
Extensive ------------------- systems --------------
----- and collect sediments.
The ------------------- becomes more compact
and -------------------
Rhizophora sp . zone (successor species)
Replace the pioneer species
The ------------------- roots trap silt and ---------------. The soil firmer, ------------------- and -------------------
------------------- submerged by sea water
Bruguiera sp. zone
Replace the Rhizophora sp.
The roots ------------------- more silt and mud.
Modify the ------------------- structure.
Terrestrial plants like the nipah palm and ------
------------- then ------------------- the bruguiera
sp.
Terrestrial forest and then a -------------------
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8.3 POPULATION ECOLOGY
THE SAMPLING TECHNIQUE TO STUDY THE POPULATION SIZE OF ORGANISM
QTA 5
Figure 6
Figure 6, show an oil palm estate. An oil palm estate is identified as a habitat for owlsand rats. The manager of oil palm estate plans to use biology control to control thepopulation size of rats which are damaging the oil palm in the estate.
Based on ecological studies, some students have carried out a study to estimate the sizeof the rats population between the area of estate. The result obtained are recorded in
Table 1
Steps in the collection ofsamples
Station X Station Y
Number of rats captured,marked and released 110 70
Number of rats recaptured 3days later
130 50
Number of marked rats 60 25
Samplingtechniques
Quadrat samplingtechnique
to study andestimate thepopulation sizeof ------------------
Capture, mark,release andrecapture
To estimate thepopulation sizeof ------------------
to estimate the ------------------- and ------------------ of organisms.
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1 (a) What technique is used for estimating the population of the rat?
………………………………………………………………………………..
[1 mark]
(b) (i) Give two characteristics of the material used for marking the rat.
……………………………………………………………………………..
……………………………………………………………………………..
…………………………………………………………………………….
[2 marks]
( b)(ii) Where will you release the rat after they are marked?
………………………………………………………………………………..
………………………………………………………………………………..
[1 mark]
(c) Why are the rat caught again after three days?
…………………………………………………………………………………
[1 mark]
(d) Estimate the population of the rat in station X and station Y.
………………………………………………………………………………………….
…………………..………………………………………………………………………
………………..……………………………………………………………………......
…………………….……………………………………………………………………
[2 marks]
(e) Give three reasons for the difference in the population of the rat in two stations.
………………………………………………….…………..……………………………………
………………………………………………………………..…………………………………
………………………………………………………………….………………………………
[3 marks]
CAPTURE, MARK, RELEASE AND RECAPTURE TECHNIQUE
Formula :(number of individuals in the first sample) x
Population (number of individuals in the second sample)
sizenumber of marked individuals recaptured
=
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THE EFFECT OF THE ABIOTIC FACTORS ON THE POPULATION GROWTHRATE OF AN ORGANISM
pH value
- the population distribution of
Pleurococcus sp. is higher at a neutral
pH
An experiment to
investigate the effect of
the abiotic factors on
the population growth
rate of an organism.
(investigate the
population distribution
of Pleurococcus sp.)
Temperature
- the population distribution of Pleurococcus sp. is
------------------- at a mild temperatures.
temperatures
Humidity
- a -------------------
humidity
is more suitable
for the growth of
Pleurococcus sp
Light intensity
- -------------------
light Intensity is
more favourable for
Pleurococcus sp.
Aspects- the aspects such as lower temperatures and low
light intensities are ------------------- for the growth of
Pleurococcus sp.
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8.4 BIODIVERSITY
Classification of organisms into the five kingdoms
Monera
- composed of ------------------- organisms
- -------------------, have cell
walls, no -------------------bound nuclei and organelles.
- photosynthetic and non-photosynthetic
- typical shapes: rod,
round,spiral
- examples :Cyanobacteria,
Kingdom
Protista
- -------------------, algae, protozoa- membrane-bound nuclei and organelles.
- -------------------, heterotrophic or both- examples :
spirogyra sp. amoeba sp. paramecium sp.
Fungi- multicellular eukaryotes
- -------------------, have
hyphae called --------------- - they have no ------------------ examples : yeast
mucor sp. mushrooms
Plantae
- all -------------- plants
- ------------------- autotrophic eukaryotes,
immobile
photosynthetic
- have ------------------- cellwalls
- examples :
flowering plants fern
Animalia
- ---------------- ------------------- eukaryotes
- well-------------------- tissues- examples : mammals,
fish reptiles
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_________________ __________________ system.
QUESTION TO ANSWER
Explain how a classification is use to name Panthera leo and Panthera tigris
The naming of organisms is based on the ------------- ------------ -------------
Each organism has ------------------- name in latin.
First name begin with capital letters refers to the -------------------, second refers to
-------------------, written in Italic
-------------------I s the genus in which the lion and tiger are placed.
However, they are of a different -------------------.
The lion belongs to the species ------------------- while the tiger belongs to the species
-------------------.
8.5 THE IMPACT OF MICROORGANISMS ON LIFE
Types of microorganisms
To which organisms do the following descriptions apply?
WTobacco mosaic virus
XYeast
YBreadmould
ZBacillus
1. Parasite
2. Saprophyte
3. Form spores
4. A multicellular organism
5. Genetic material is a single strand of RNA
6. Reproduces by binary fission
Organisms Human Wolf Frog
Genus Canus Rana
Species lupis pipiens
Scientific name
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THE EFFECTS OF ABIOTIC COMPONENTS ON THE ACTIVITY OFMICROORGANISMS
ABIOTICCOMPONENT
EFFECTS
Temperature - ------------------- at low temperature
- the optimum temperature is between -------------------
- beyond ------------ the growth of microorganisms is inhibited
- microorganisms and their spores can be ------------------- at
a temperature of about 121°C
The activity of yeast is optimal at a temperature of --------.
Light intensity - prefer ------------------- or low light intensities
- ------------------- intensities of sunlight or ultraviolet rays can
------------------- microorganisms
- ------------------- and photosynthetic bacteria need light to
photosynthesize
The activity of yeast is higher at a ------------------- intensity of light.
pH value - most bacteria prefer slightly ------------------- conditions .
pH ------
- moulds, yeast and protozoans prefer acidic conditions
(pH 4.5 – pH 5.0)
- extreme pH can kill the microorganisms
The activity of yeast is optimum in the -------------------medium.
Nutrient - all microorganisms except ------------------- need nutrients and
water for reproduction and growth
- without nutrients or water, microorganisms will die or
form -----------------
The higher the concentration of nutrients, the ---------------
the activity of yeast.
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QTA 6 :THE IMPACT OF MICROORGANISMS ON LIFE
Figure 7 : The impact of microorganisms on life
Microorganisms are found everywhere on the surface of the earth. Many types of
microorganisms are harmless and are actually quite beneficial to humans.
(a) State 5 types of microorganisms found on earth.
1.…………………… 2. …………………… 3.………………… 4.……………………… 5……………………
[2 marks] (b) With example, explain the importance of any 3 types of microorganisms.
……………………………………………………………………………………………….
………………………………………………………………………………………….
………………………………………………………………………………………….
………………………………………………………………………………………….
[3 marks]
(c)(i) From Figure 7 name 2 groups of nitrogen fixing bacteria and state briefly their
way of life.
……………………………………………………………………………………….
[3 marks]
(c)(ii) What is the meaning of nitrogen fixing bacteria?
………………………………………………………………………………………
………………………………………………………………………………………
………………………………………………………………………… [2 marks]
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(d) What is compound x ?
………………………………………. [ 1 marks]
(e) What is the use of microorganisms in biotechnology ?
………………………………………………………………………………….
…………………………………………………………………………………
………………………………………………………………………………..
[ 2 marks]
(e) What is the importance of preservation and conservation of biodiversity ?
…………………………………………………………………………………. …………………………………………………………………………………
………………………………………………………………………………..
[ 2 marks]
GUIDED ESSAY :THE ROLE OF USEFUL MICROORGANISMS
1. Decomposition
a. Carried out by a group of ------------------- bacteria and fungi, called ---------------
b. Secrete ------------------- that break down complex organic nutrients into ------------------- inorganic substances such as carbon dioxide, water and -----------------
this reduces pollution by preventing the accumulation of the remains of
decaying organisms.
c. Maintain life on earth by recycling and ------------------- nutrients into the soil.
2. Digestive system in human
Symbiotic bacteria in human ------------------- synthesise vitamin B12
and -------------------
Deficiency in vitamin B12 lead to anaemia, ------------------- needed forblood clotting.
3. Alimentary canal of termites
Termites feed on ------------------- which contains cellulose, they do not
have the enzymes (-------------------) to digest the cellulose.
Trichonympha sp. is a mutualistic protozoans present in the alimentary
canal of termites and secrete -------------------.
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4 Nitrogen cycle
a. The nitrogen cycle allows nitrogen to be utilized by organisms and ---------------
to the physical environment.
b. Plants can only ------------------- nitrogen in the form of -------------------, nitrite or
nitrate ions.
c. Nitrogen fixing bacteria such as -------------------. (lives in the soil) and ------------------- (lives in the root nodules of leguminous plants) can convert atmospheric
nitrogen into ------------------- compounds (NH3 and NH4) through a process
called -------------------.
d. Nitrates are taken up by the roots of plants and converted into plant -------------
When the animals eat the plants, the organic nitrogen is transferred into the
body of animals and becomes animal protein
e. Waste matter, ------------------- and animals which die and decompose are
converted into ammonium compounds.f. Ammonium compounds are converted into -------------------and nitrates by ------
------------- through a process called -------------------.
g. Ammonia converted into nitrites (NO2-) by -------------------
h. Nitrites converted into nitrates (NO3 ¯) by -------------------.
i. The cycle is balanced by a continuous return of nitrogen to the atmosphere by
-------------------which break down nitrates and release nitrogen back into the
atmosphere.
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The effect of harmful microorganisms1. Harmful microorganisms can ------------------- the food and substances.
2. Microorganisms that cause diseases are called -------------------.
3. Organisms that transmit the pathogens are called --------------------------------------.
How the diseases spreadi) Direct contact- a contagious disease can be spread by ---------------- with an infected person or
using their personal items such as ---------------- clothing.
ii) Airborne and droplet transmission
- airborne transmission is a method where the pathogens can change into -------------
and then transmitted by ---------------- droplet transmission is a method where
liquid
Airborne and droplet transmission
- airborne transmission is a method where the pathogens can change into --------------
and then transmitted by -------------- droplet transmission is a method where
liquid
Food and water
- microorganisms enter the alimentary canal through contaminated food and water,
------------- hands
Disinfectants
- solutions used to -------------------
surgical equipment, kill
microorganisms on the floor.
Examples : phenol, formaldehyde
Vaccines
- a suspension of ------------------- or
------------------- bacteria or viruses
which is inoculated into the body
to induce the production of
-------------------. Example : BCG
Antiseptics
- used on cuts and ------------------- to
kill and inhibit the growth of
microorganisms. Examples :
acriflavin, -------------------
Antibiotics
- Penicillin and streptomycin are
produced by microorganisms
which inhibit the growth or ------------
other microorganisms, esp.
bacteria
Methods of controlling pathogens
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GUIDED QUESTION PAPER 3 :QTA 7: Complete the report below
Objective: Estimating the percentage coverage of plants using the quadrat
sampling technique.
Problem statement: What is the percentage coverage for plant species P and Q
in the selected sample?
Hypothesis : …………………………………………………… ..
Variables
i. manipulated: …………………………………………………..
ii.responding: …………………………………………………….
iii. constant: …………………………………………………….. Apparatus: 1m X 1m quadrat
Technique: To estimate …………………………………………..
Procedure:
1. Survey and select a suitable sampling area.
2. Identify two plant species, P and Q to be sampled in the area.
3.Place ten square quadrats, each 1m2 on the sampling area randomly.
4. Divide each quadrat into ………. small quadrat using nails and strings.
5. Each small quadrat measures ……………. (The size of each small quadrat is
…………….)
6. Count the number of small quadrats for the plant species P. (Count only the
small quadrats that are covered ………. or more by the species.)
7. Count the total area covered by the plant species P by multiplying total number
of ……………………… covered with the area of one small quadrat (0.01 m2).
8. Repeat ………………………………………………………………….
9. Record the observation in the table below.
10. ……………….. the percentage coverage of species P and Q using the
formula below
Percentage coverage = Total area covered by the species X 100%Number of quadrats X area of one quadrat
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Result.
Species Area covered by the species in each
quadrat (1m2)
)
Total area
covered by
the species
(m2)
Percentage
coverage %
1 2 3 4 5 6 7 8 9 10
P
Q
Conclusion: ………………………………………………………………………………
……………………………………………………………………………………………..
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GUIDED QUESTION PAPER 3: QTA 8
Lemna minor is a species of free-floating aquatic plants from the duckweedfamily Lemnaceae. The plants grow mainly by vegetative reproduction: twodaughter plants bud off from the adult plant.
An experiment is carried out to investigate the effect of abiotic factor such aspH on Lemna sp. growth. Experiment is done under controlled conditions: 12hours a day light exposure and using the same Knop’s solution.Petri dish is filled with 20 ml Knop’s solution with different pH value and 5Lemna sp. each.The Knop’s solution is treated by adding acid or alkali to achieve the pHvalue needed.
** Knop’s solution is a solution which contains essential nutrient for plantsgrowth.
Figure 1
After 7 days, the observation is made and the result shown in Table 1..
Lemna minor
Petri dish
Knop’s solution
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Table 1
pH
valuePetri dish Number of Lemna sp.
2
4
6
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8
10
12
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Based on the experiment, answer all questions below.
(a) State the number of Lemna sp. in the spaces provided in Table 1.[3 marks]
(b) (i) Based on Table 1, state two observations that can be made in thisexperiment.
Observation 1: ……………………………………………………………..
………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
Observation 2: ……………………………………………………………..
………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
[3 marks](ii) State the inference for each observation made in (b) (i).
Inference for observation 1:………………………………………………
………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
Inference for observation 2: :……………………………………………
………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
[3 marks]
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2. Based on the experiment, define operationally the abiotic factor in anecosystem.………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
………………………………………………………………………………
[3 marks]