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Early Detection of Highly Pathogenic Viral Infections Ignacio Sanchez Caballero Wednesday, May 1, 13
| Date post: | 09-May-2015 |
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Early Detection ofHighly Pathogenic
Viral InfectionsIgnacio Sanchez Caballero
Wednesday, May 1, 13
Infection
Wednesday, May 1, 13
Viremia
Infection
Wednesday, May 1, 13
Viremia
Infection
Transcriptional changes
Wednesday, May 1, 13
Lassa
Marburg
17
1 2 3 4 5 6 7 8 9 10 11 120
4 6 3 6 3
15 3 3 3 3 3
Days post-exposure
Number of Samples
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Lassa Marburg
Samples taken at the same time have similar gene expression values
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Combined
Samples taken at the same time have similar gene expression values
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30
High expression
Low expression
Days post-infection
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No change
Increase in expression
Decrease in expression
30
High expression
Low expression
Days post-infection
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No change
Increase in expression
Decrease in expression
Probably random Probably significant30
High expression
Low expression
Days post-infection
Wednesday, May 1, 13
No change
Increase in expression
Decrease in expression
Probably random Probably significant30
High expression
Low expression
Days post-infection
Significant high magnitude change
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No change
Increase in expression
Decrease in expression
High expression
Low expression
Days post-infectionProbably random Probably significant
Significant high magnitude change
30
Wednesday, May 1, 13
No change
Increase in expression
Decrease in expression
High expression
Low expression
Days post-infectionProbably random Probably significant
Not significant high magnitude change
30
Wednesday, May 1, 13
No change
Increase in expression
Decrease in expression
High expression
Low expression
Days post-infectionProbably random Probably significant
Not significant high magnitude change
30
Wednesday, May 1, 13
No change
Increase in expression
Decrease in expression
High expression
Low expression
Days post-infectionProbably random Probably significant
Significant low magnitude change
30
Wednesday, May 1, 13
No change
Increase in expression
Decrease in expression
High expression
Low expression
Days post-infectionProbably random Probably significant
Significant low magnitude change
30
Wednesday, May 1, 13
A subset of probes is differentially expressed 3 days post-exposure
Wednesday, May 1, 13
A subset of probes is differentially expressed 3 days post-exposure
Wednesday, May 1, 13
Wednesday, May 1, 13
Wednesday, May 1, 13
Wednesday, May 1, 13
Lassa
Marburg
4
1 2 3 4 5 6 7 8 9 10 11 120
2
1
Days post-exposure
Number of Samples
1
Test data set
Wednesday, May 1, 13
Lassa
Marburg
4
1 2 3 4 5 6 7 8 9 10 11 120
2
1
Days post-exposure
Number of Samples
1 ✖
Test data set
Wednesday, May 1, 13
Aim 1: Early detection of highly pathogenic viral infections
Compare the early transcriptional response of Lassa and Marburg✓
Wednesday, May 1, 13
Aim 1: Early detection of highly pathogenic viral infections
Compare the early transcriptional response of Lassa and Marburg✓
Identify a set of candidate biomarker genes✓
Wednesday, May 1, 13
Validate the current set of biomarkers using qPCR
Aim 1: Early detection of highly pathogenic viral infections
Compare the early transcriptional response of Lassa and Marburg✓
Identify a set of candidate biomarker genes✓
Wednesday, May 1, 13
Validate the current set of biomarkers using qPCR
Compare the differences in using sequencing to measure gene expression
Aim 1: Early detection of highly pathogenic viral infections
Compare the early transcriptional response of Lassa and Marburg✓
Identify a set of candidate biomarker genes✓
Wednesday, May 1, 13
Validate the current set of biomarkers using qPCR
Compare the differences in using sequencing to measure gene expression
Incorporate additional viruses to the analysis
Aim 1: Early detection of highly pathogenic viral infections
Compare the early transcriptional response of Lassa and Marburg✓
Identify a set of candidate biomarker genes✓
Wednesday, May 1, 13
Aim 2: Visualization of complex biological data
Develop a software tool to easily generate interactive plots✓
Wednesday, May 1, 13
Aim 2: Visualization of complex biological data
Develop a software tool to easily generate interactive plots✓
Make generating a dynamic plot as easy as a static one
Wednesday, May 1, 13
Aim 2: Visualization of complex biological data
Develop a software tool to easily generate interactive plots✓
Make generating a dynamic plot as easy as a static one
Aim 3: Experimental training
Develop core skills in experimental virology
Wednesday, May 1, 13
Aim 2: Visualization of complex biological data
Develop a software tool to easily generate interactive plots✓
Make generating a dynamic plot as easy as a static one
Aim 3: Experimental training
Develop core skills in experimental virology
Design and carry out experiments to determine viral and host gene function
Wednesday, May 1, 13
