1
Easy microbial
monitoring of entire
chicken flocks
November 2019 | Dr. Anita Menconi
Evonik at Poultry Tech Summit 2020
Atlanta, GA
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EvonikWhat we don't do
NUTRITIONTOWELS TABLETSCAR TIRES MATTRESSES
What we doLiving better with Evonik
more effect iveheal th ierf lu ff iermore fue l saving more e las t ic
Countless products gain their special attributes through our creative power.
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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Our structureStrategic concentration and operative independence
Performance Materials
Polymer materials and intermediates mainly for the rubber, plastics and agriculture industries
Nutrition & Care
Products for use in the areas consumer goods, nutrition and health
Resource Efficiency
Environmentally friendly and energy-efficient systems as solutions for several industries
Technology & Infrastructure
Site operation, energy & utilities, technical services, logistics, process technology and engineering
Segments
Strategic Management Holding
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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Evonik Animal NutritionThe core: Amino acids and Gut Health Solutions
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
Services▪ MetAMINO®
▪ Mepron®
(rumen
protected
methionine)
▪ AQUAVI®
Met-Met
▪ Biolys®
▪ ThreAMINO®
▪ TrypAMINO®
▪ ValAMINO®
Amino
acids
▪ Ecobiol®
▪ Fecinor®
▪ GutCare®
▪ GutPlus®
▪ AMINOLab®
▪ AMINODat®
▪ AMINONIR®
Advanced
▪ AMINONRG®
▪ AMINOProx®
▪ AMINORED®
▪ AMINOInsight®
▪ AMINOBatch®
▪ AMINOBatch®
WPT
▪ AMINOSys®
▪ AMINOCHICK®,
AMINOHEN®
▪ AMINOPig®
▪ AMINOCow®
▪ AMINOShrimp®,
AMINOTilapia®
▪ AMINOFootprint®
▪ Animal nutrition &
Gut health
consulting
The shell: Software and services combined
Amino
acids
The completion: nutrition and health concepts
Standardized
ileal digestibility
(SID)
Net
energy
systems
Low
protein
diet
Nutritional
recommen-
dations
Amino
acids
n
The holistic approach: precision livestock farming (PLF)
Amino
acids
▪ FloXControl®
▪ ScreenFloX®
▪ Stratify individual farm animals or
flocks to enable optimized
management and feeding
▪ Improve, track, and trace animal
welfare and sustainability
▪ Benefit from artificial intelligence,
cloud solutions, and Internet-of-
Things
5 | November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
A new way of thinking flock health
▪ Current approach: Individual animal, infectious diseases (clinical), uses invasive methods
▪ Health focus to sustain a livestock
▪ Evonik screening approach: entire flock, use of non-invasive methods to assess the status of a livestock herd/flock
▪ Fast turnaround time due to on-site
▪ The only system solution (reliable, robust) that can be expanded to other parameters and enables customer to optimize
their production process.
Common
practice
New
approach
Probiotics, ….
By veterinarian:
e.g. therapeutical
antibiotics
Intervention by medication
Prevention / soft intervention
6 | November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
How do we achieve this?From single bird diagnostic towards a powerful herd approach
Pick a few →
Diagnose each individuum
Collect feces randomly, representing the flock by
pooling into one sample
Diagnostic
Test/Device
Environmental
Screening Test
~ 6 x
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Evonik microbial screening PCR platform “ScreenFloX®”: addressing various customer needs via several tests
*PCR = polymerase chain reaction (genome cloning and sequencing tool)
**One-for-all workflow (identical settings)
Human
pathogenic
Avian
pathogenic
Control of
livestock
production
process
On-farm workflow** (6 hours to overnight)
Result/InterpretationAnalysisPre-treatment Sample Prep.Sample Collection React
• Machine settings
identical for all tests
• All tests use the same
collection & sample
preparation chemistry
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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Sample collection and laboratory assay – Workflow
Heating Grinding Spinning Extraction qPCR
Sample collection @ study site
Perform assay @ lab site → 3 hours
- 5-20 g of fecal material / per tube
- Storage at RT/-20°C up to 7 days
- Extraction and qPCR device fixed
- SOP – workflow
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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Utilizing PCR as a screening solution requires standardization
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
Customer Requirements:
A screening device must be:
• Reliable
• Reproducible
• Accurate & precise
• Specific & sensitive
• (Quantitative)
Evonik‘s Approach:
Reduction of variances:
• Control the sample
• Standardize workflow
• Use of premium quality
chemistry
• Superior production
process (ISO 13485)
Reduction of errors:
• Automated workflow
• Use of internal run control
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Functional design of the ScreenFloX® PCR C. perfringens DNA test (quant.) –The Kit allows easy monitoring of health and microbial status of entire flock
Detection React
Recommendation
• Soft intervention
• Treatment decision
Status of the flock
B A
B A
Ratio 0,8
Ratio 0,1
Interpretation
‚Healthy‘
’Affected’
Act
Caution
OK
Act
Caution
OK
A B
A B
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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Testing Kit performance in the field - ScreenFloX® for Clostridium perfringens
NE outbreak
(pathology confirmed)
netB cpa
control
(no pathology)
Day
17
For each flock 96 randomly collected samples were pooled into one sample that was further analyzed
4 flocks each tested
• All samples contained Clostridium perfringens - tested positive through cpa
• All NE-positive flocks were tested netB positive
• All NE-negative flocks were tested netB negative
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
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ScreenFloX® at a Glance
| November 2019 | Easy microbial monitoring of entire chicken flocks | Poultry Tech Summit | Dr. Anita Menconi
Performance
Trust
Openness
Speed
• Quantitative real-time PCR tests with
high linear range
• Highly sensitive, specific, accurate and
precise
• Robust, reliable
• Validated system solution to minimize
variances (workflow, internal control,
etc.) → optimized workflow
• Representative (whole flock)
• Sample to result: 3 hrs*
* Depending on numbers of samples processed and the customer set-up
• Scalable: up to 80 samples per shift
• Expandable: Up to 5 different tests at
any time on the instrument. Unlimited
test menu (viruses, bacteria, protozoa)
• Compatible: Modular nature makes
system compatible to other workflows
ScreenFloX® is a molecular screening solution to monitor the microbial burden of herds in order to improve the
production process and de-risk livestock production
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