AN INNOVATIVE - EASY TO USE TOOL

– TO SECURE MALOLACTIC

FERMENTATION IN BARRELS

3 common formulations of wine lactic acid bacteria

for induction of MLF in wine

LIQUID FROZEN FREEZE-

DRIED

Storage / Shelf-

life

4°C: 2 weeks - 2

months

-45°C: 1 year 4°C: 18 months

-18°C: 36 months

Stability Not stable

Not stable except

at -45°C

Most stable form

Transport Cold

Cold (-45°C) Cold, but tempera-

tures variations are

tolerated

Risk of

contamination

High Low Low

Direct inoculation – MBR®

The MBR® process was developed by Lallemand to reinforce the

Oenococcus oeni cell membrane and to adapt the cells to these harsh

conditions to accomplish direct inoculation.

Direct inoculation is possible or a simple rehydration before inoculating

the wine.

Without rehydration With rehydration OR

Protocol of use : MBR®

To facilitate mixing, we recommend the following: Rehydrate the packet of freeze-dried wine bacteria in 20 times its

weight of clean chlorine free water at 20°C for a maximum of 15 minutes.

Add the suspension directly to the fermenting wine or in the wine towards the end of AF, then stir gently to evenly distribute the bacteria and minimize the oxygen pick-up.

But direct inoculation without rehydration is also possible:

Open the sachet and add the wine bacteria without rehydration prior to or during a pumping-over:

- directly into the fermenting must during a pumping-over (co-inoculation)

- directly into the wine after the end of alcoholic fermentation (AF) or while emptying the tank (post AF-fermentation)

NEW

Effervescent tablets for barrels

Pilot and industrial trials: good repartition of bacteria into the tank - same efficiency than

our current freeze-dried MBR bacteria

No need for rehydration or pumping-over to suspend the bacteria

Easy dose and use: 1 tablet for 1 barrel

MALOTABS

Formulation : MBR bacteria / microcrystaline cellulose / potassium bicarbonate

TRIAL -2013 Chardonnay - Dijon

• AF with CY3079 YSEO®.

• Sequential inoculation with selected wine bacteria. MLF at

20°C. 4HL

• Different bacteria and forms

• bacteria S1 MBR ® : with rehydration = S1R

• Bactetia S1 MBR®:no rehydration= S1

• Bacteria S1 in Tablet form = S1 TAB

• + two other trials with bacteria S2 direct inoculation (S2 R) or tablet

form (S2 TAB)

Kinetics of malic acid degradation in a 2013 Chardonnay (Dijon) – pH 3.2, 12.65 %v/v, free SO2 < 10 mg/l

Days

Mal

ic A

cid

(g

/L)

No difference of MLF speed between the different

forms with bacteria S1

S1R

S1

S1TAB

S2R

S2 TAB Mal

ic A

cid

(g

/L)

S1R

S1

S1TAB

S2R

S2 TAB

days

2013 MLF Trial MLF in Lemberger (Württemberg)

Inoculation post AF comparing direct inoculation with bacteria B6

in powder and in tablet form

0,00

0,50

1,00

1,50

2,00

2,50

3,00

3,50

29/10/2013 31/10/2013 02/11/2013 04/11/2013 06/11/2013 08/11/2013 10/11/2013 12/11/2013 14/11/2013

ma

lic

ac

id (

g/L

)

Kinetics for malic acid degradation

VP41 tablet

VP41 powder

S1 TAB =

Malotabs

S1

No difference of the speed of

MLF = very good dissolution

and dispersion of MATOLABS

Pinot Noir Germany 2016

• comparison of different Lallemand Bacteria preparations on the speed of

MLF - sequential inoculation (= post AF inocualtion) in barrels .

Code Experiment

01a/b Control – spontaneous MLF

02a/b Sequential inoculation Lalvin VP41

03a/b Sequential inoculation Uvaferm Alpha

04a/b Sequenti3al inoculation MLPrime

05a/b Sequential inoculation PN4

06a/b Sequential inoculation MALOTABS

traditional fermentation Pinot Noir Germany 2016 – pH 3.45

Kinetics of malic acid degradation

Good kinetic and efficiency of MALOTABS

Code Experiment

01a/b Control – spontaneous MLF

02a/b Sequential inoculation Lalvin VP41

03a/b Sequential inoculation Uvaferm Alpha

04a/b Sequenti3al inoculation MLPrime

05a/b Sequential inoculationPN4

06a/b Sequential inoculation MALOTABS

FEED-BACK from the markets Good efficiency of the product : good start and

complete MLF

Good dispersion and no need of mixing or stirring

Easy to use for low volumes (barrels or small tank of wines)

Long Shelf life

https://www.youtube.com/channel/UC8FsRZ_ARG_R2CGnz0y_cqw

Video 5

file:///C:/Users/skrieger/Videos/Video-Teile Weinsberg.movfile:///C:/Users/skrieger/Videos/IMG_1184.MOVhttps://www.youtube.com/channel/UC8FsRZ_ARG_R2CGnz0y_cqwhttps://www.youtube.com/channel/UC8FsRZ_ARG_R2CGnz0y_cqw

Innovation is recognized!

Intervitis-Interfructa 2016 SIMEI – drinktec 2017

Innovation is recognized!

Intervitis-Interfructa 2016 SIMEI – drinktec 2017