Ecosystem Approaches to the Better Manageme nt of Brucellosis and toxoplasma in Yunnan Pro

vince, ChinaYANG Guorong Yunnan Academy of Grassland and Animal Science, YAGASYANG Xiangdong Yunnan Institute of Endemic Disease Control and Prevention, YIEDC

YANG Shibiao Yunnan Academy of Veterinary and Animal Science, YASVILI wengui Yunnan Agricultural University, YAULI Ruisheng Animal Husbandry Bureau of Mangshi CityYANG ZhiminHE YongZhen

Animal Husbandry Bureau Of Yiliang countyYunnan Academy of Grassland and Animal Science, YAGAS

FANG Jing Kunming Medical University

EcoZD annual meetingEcoZD annual meeting Bangkok 1Bangkok 100--1212 DeceDecember 201mber 20111

Background An ecosystem approach to human health is an new

approch - . This multi disciplinary field of study invest igates how changes in the earth's ecosystems

are impacting human health so as to prevent new diseases from emerging, particularly by

building more equitable and less ecologically damaging agricultural production systems.

Project in China focus on brucellosis and toxoplasm osis– both from animal and public health perspecti

ves, and to measure the impact of these diseases i n pilot communities; thereafter to prevention and

control two disease in people and livestock.

MangshiYiliang

Mekong River

Research objectives and hypothesis Hypothesis: Brucellosis and Toxoplasmosis are under-reported disease in both

humans and livestock in Yunnan province. Animal and human health personnel do not have experience or diagnostic capacity to confirm cases; communities also are unaware of the risks of disease.

Objectives: To understand perception, awareness and behaviour of stakeholders

including potential risk groups towards these zoonoses To investigate the impact of these diseases in pilot communities,

hospitals To demonstrate how an integrated approach can help to improve

collaboration between different stakeholders and contribute to a better control

Signing and laun ching of EcoZD

- Dec 12 13, 2010 in Kunming

6

Groups involved

The problem: Brucellosis & Toxoplasmosis in Yunnan

Public health authorities(hospitals)

Vet officers & Vet stations

Local administration officers

Farmers , pet owners •cattle, buffalo, small ruminants,

pigs, cats•Small holder and cooperatives

Past unit, milk vendors

Villagers (ethnic minorities) Butchers

Consumer

Research methodology

Obj 1: Understand perception, awareness and behaviour of stakeholdersliterature reviewFGD, Questionnaires, Interviews (ie farmers, villagers, butchers, vets , PH, past units ect)

Obj 2: Impact of B & T in pilot communities (animal and humans) literature review and historical data stored in involved partner institutions Pilot surveys to determine the sero-prevalence in pilot communities (standard tests applied) Animal sector: dairy goats, dairy buffalo/cattle, cooperative farms)(vet station case review)Human: risk groups, hospitals (review of cases)

Obj 3: Added value of an integrated approachJoint design, implementation, analysis & publications

Progress made Selected results Animal production Data has been collected and analyzed in Yiliang and

Mangshi project Investigation of Animal Toxoplasma gondi (YAGAS) Serological results for Toxoplasma (YAU)

Site HostNo of serum

samplesNo of Positive

Positive rate(%)

method

Yiliang

Pig 109 16 14.68 IHA

cattle 9 1 11.12 IHA

cat 5 2 40.00 IHA

Hilar lymph nodes of pig

16 3 18.75PCR

Mangshi

pig 128 57 44.53 IHA

cattle 44 8 18.19 IHA

cat 3 2 66.67 IHA

hilar lymph nodes of pig

57 20 35.09PCR

Serological and molecular investigation on prevalence of toxoplasma

Progress made: Prevalence of Brucellosis in Animals in Yunnan province,

China(YASVI)(*retrospective data and **collected data within EcoZD)

Host samplling site methods used

positive rates

Pig* Yunan province ELISA 0.40%(N= 22,213)

cattle/buffalo* Yunan province ELISA 0.76%(N=37,033)

dairy cow** Mangshi ELISA 0%(0/11)

dairy water buffalo**

Mangshi ELISA 0%(0/95)

goat/sheep* Yunan province ELISA 2.55% (N= 53,215)

culture and isolation of pathogen ， ASVI 2010

pathological examination, Dali, 2010

Collection of buffalo tissue samples in slauterhousre, Mangshi ， 2010

Field investigation on Brucella in dairy buffalo farm ， Mangshi, 2010

Prevalence of Tox and Bru human in Yunnan provin ce, China (YIEDC)(collected within EcoZD)

site target disease method used

positive rate

Yiliang BruTox

ELISASAT

0%(0/132)33.3%(44/132)

Mangshi BruTox

ELISASAT

0%(0/107) 71.0% （ 94/132）

L ab analysis of Brucella samples

Planned activities for 2012 and beyond 1

o More in-depth animal surveys (production data, productive disorders, bio-security, perception on these 2 zoonoses)o Done in conjunction with Human sector (YIEDC) covering target

township and villages in Mangshi and Yiliang o Animal survey will focus on dairy c ows, milk goats and wat

er buffalo (backyard and cooperatives farms) .o Also historical prevalence data will collect in vet station.

o Public health Human disease survey (risk groups, done in

conjunction with animal survey/partners)o Human case survey (Toxoplasmosis)o Hospital records review investigations (Bruc & Toxo)

Planned activities for 2012 and beyond 2

In addition to the animal survey, data on perception, behavior

and awareness of various risk groups will be collected by Que

stionnaire, Interviews and Focus Group Discussion.

Identification of possible risk factor.

Through training (ie participatory methods) and regular

meeting to enable team member familiar with the conce

ption of Eco health and to improve the ability of using

social research methods like Outcome Mapping, FGD

Expected outputs and outcomes

Researchers will have gained new skills and knowled

ge from concurrent training programme including Ec

oHealth approach, Outcome Mapping (a component t

ool), Proposal Design, & Research Methods.

Evidence will be generated on zoonotic EID from eac

h research team that can inform research priorities a

nd decisions for priority zoonoses including EIDs

3 Papers, 3 reports (2 annual, 1final)

Lesson learned/reflectionWhat we Learn From OM

Got a methodology for planning and assessing development programming that is oriented towards change and social transformation.

our project helps us to learn about its influence on the progression of change in our boundary partners, and therefore helps us adaptively manage variations in strategies to bring about desired outcomes.

This method is useful both for EcoZD and other projects. Challenges face with Still unfamiliar with Eco-health research method & Communication

limitations between project partners but also with ILRI. due to the delay of project funding, part of the project work wasn't

carried out smoothly.

Perspectives

• We will apply for what we have learnt in this project such as Ecohealth Approaches, Outcome mapping, and Focus Group Discussion in other projects and programs

• We will contribute to the building of EcoHealth network in Yunnan and China.

Ecosystem Approaches

Sampling of bufalo blood （ Dehong ）， 2010

Communication with farmers （Dali ）， 2011

Sample recording （Dali ）， 2011

A training workshop on health farming and major diseases

control in cattle in total 4 counties, Yunnan, Oct-Nov,

2011

- Interview of high risk oc cupational group o f Brucellosis

Training on preven tion and control of Brucella

YIEDC team

25

An interview at Vet station (Dali, 2011)

26

Interview of Family scale diary cow farmers (Dali, Aug 9,2011 )

ILRI EcoZd Kunming Workshop - March 15 18, 2011 Kunming

ILRI EcoZD workshop - 712 2011Aug , (Dali &

)

ILRI EcoZD workshop - KKKKKKK25 29, 2011