Any TWO courses from the following(Zoo 311 to Zoo 314)EnzYlllologySystematics and Paleontology andOrganic Evolution.Paraai toloSYBiophysics.
80+20 mke Int. Ass.
80+20 mks Int. Ass.
80 mks + 20Int. Ass.
Paper-3
zoo 315ZOO 316ZOO 317ZOO 318ZOO 319ZOO 320
Any two from the following(Zoo 315 to Zoo 320)Emdocr inolOIYApplied Entomo1or,.AquicaltureInsect PhysioloryComputer Progr~lllingTaxono.y. distribution and bionomics of fishes.
.k.
ZOO 325 Practica1-I18 Practicah w.r.t. ZOO 301 to ZOO 3057 Practical; Of.r.t. ZOO 311 to ZOO 314
IParasitic and sedentary crustacea.Cephalization in Arthropoda6.
7 •
Ectoprocta -
Arthropoda
Mollusca
Branchiopoda, Cbaetogna - the, Phoronida. Generalorganization and structure.
Torsion and detorsion
8. Echinodermata Larval forms Structure and development.
Hemichordata Affinities and interrelationships,
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1. Aaina acids and proteine : MethodJl ot Isob.tion and purification.2. Nucleic acid: ehe.latry in brief, Bia-synthesis of purines and
pyriaidines, isolation of nucleic acid and characterization.
.'<
3. Carbohydrates Structure at glycogen,degradation, Polariaeter.
1.
4. Lipida Chs.18try in brief, Clusification, properties andBeta-oxidlLtion.
5. Biocheaieal soerleties : Concept of free energy, Redox potential,Energy rich Co.pounds, ATP, ADP, GTP and Co-A. Coupling ofendergenic and exergenic reactions with reference to Kerb's cycleand Oxidative phosphorylation.
6. Radioactivity deter.ioation Properties of Alpha, Beta, Gaa&rays. Radio active isotopes, half-life period, Units ofradioactivity, GM-Counter, 8010t1118t100 counter, Applications ofRadioiaotopes and Autoradiograpby.Techniquen and Inatru.entation. Analytical Biocbe.latry - Hitbspecial reference to Bio-Micraeoleculea, proteins, enzyaes andNucleic acida.Colorl.,t"colori.,ter,
Beer's and Laabert'n law, easentialSingle beaa and double beall.
8pectMMPPJ' : Bingle, Double ben. and UV - spectra.etrr.CantriCg'ttlog : Analytical preparation, Denaitr gradient,Gentrifq",ipn, Zonal centrifugation.ChroMtwapbr : Paper chroutography (two diRntiona1), factonaffecting If. Gel filtration, Principle, different supporting.edia. Affinity Cbroaatograpby - Principle, ion-exobangesbNlMto.rphy - Principle8 of trpe.Ilegtrgpborqli. - Principlen of paper and gelelectrophoroaia.
8. Techniques in M&nolletr:r. SQ,Warburl apparatus, applications
088 cODatant,of Mane.etry.
flank CODatant,
Protozoon UltraetructureProtolloololYProtozoologyTbe Tre.atodaIntroduction to ProtozoaTbe Molluac&PresobrlUIcb MolluscaTbe Basic Artbropodan alockPhysiologJ of CruataceaA Treatlze on Zoolol:r (Part-I-IX)Caabridge Natural Hialor:r
•
D. PitelkaP.R.KudeI.P.HallB.ne"asD. MaokinnonJ.E.MortonK.M.Wilbur and C.M.YoungA.O.SharovT .H.Water.anH.Ray Lankeater (Ed)S.F.Haraer andA.E.Sbiple:r (id.)
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Biology of CrustaceaPalaeontologyPalaeontologyEchinodenataPogonophorll.raplications of EvolutionThe lower MetazosStudents text books of ZoologyVol. I-IIIThe Invertebrates
Protozoa through CtenophoraPlatybelainthis - RhyncoeoelaAcanthocephala - EntoproctaBchinod"nataSaaller Coela.ate GroupsMolluDCIL
REFERENCE ~ lQR BIOC!fE)JIURY
Segar loR. (1976) - Biocheaie&1 CalC\lbtion, 2nd, Elin. Iii ley, NellYorkwillard, H.R.Merril, LL and Dean, A.J. (1974) - Instrumental Methodsof Analysis, 5th Edo. D.Van. Nostrand Co., New York.Lehniger, A,L. (1976) - Biocheaistry, 2nd Edo. Worth Publ. New YorkMahler, H.R. and E.H. Cordes 1971, Biological Chemistry, 2nd!dn NewYork, Harper and Row.Stryer, Lubert 1975 Biocheaiatry San Francisce, W.H.Freeaan IlJIdCoU.breit, W.W.R. Burris & J.F.Staufrer, 1957 Manoaeric techniques,Menneapolics. Burgess.Harper's(reprint)E.E.Conn.G,H.Bell,Physiology
Review of Biocheaistry Long Medical Pabl. CaliforniaBiochemistry ediated by Zubay, Addison Weslay (1983),l P,K.Stulli (1978) ~ OUt lines of Biochemistry, 4th Edn.D.Easlie. Smith and C.R. Paterson (1976) 'Fext Book ofand Biochellistry 9th Edn ELBS LiVingstone.
1.
zoo lil1 ENVIRONMENTALBIOLOOY
Review of Ecology of natural systells ~ Biosphere, biotic andabiotic components, integration of biotic and abiotic factors,Food wed and trophic leveb, carbon, nitrogen and phosphorouscycles.
2. Changes in Ecosystells : Succession, long range stll.bility ofecosysteas and of atllOsphere.
3. Structure of freshwater cOlllll1llity- Lakes origin, verticalstratification, thermal exchange, dissolved gasses, salts etc.Plankton - Phyto snd zooplanktons of lotic and lentic systelll",seasonal fluctuations, productivity of freshwater ecosystem.
•• Structure of terrestrial cOlllmunitybiomass, productivity.
Terrestrial perlliants
5. Environlllentaladaptations ~ Structural and functional adaptationswith reference to freshwater, aarine, terrestrial, desert, aDOWbiollaas etc.
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5. Air and water pollutions :.} Air pollution Sources industrial,
c~buatlDnal. Effects on anlaale (includingpl!ll1ts. Prevention and control Ruuree.
vehicular,hulll:Ul) and
Water pollutionwastes, paper andand distilleryindustrial waste,
Major industrial effluents,pulp industry wastes, dairy waate,
wastes, pharaacutica! Ifastes,Tre.taeot tor water pollution.
huards, evaluation and COQtrol. natural and artificial,
b) Noise pollution - Sources, eftects on busan, prevention.8. Energy: Reaources and coDau.ption, energy crieis.9. Huean popullatioDs Defioetion, population, growth curves,
Datality and »orality, world wide de.agr8phic trends, populationdensity, poul.tioD explosion and Ite i.pect on environ.ent,population control.
10. Coneervation of nature: Speciee and extinction of epecie. _Deatruction of habitat, introduction of foreign Bpeciee,co••erciBl harvest, predation, Action to save endangered species
Research and docu.entation, habitat preservation anddevelop'ent of wild life refuge, providing critical resources,legal actionl for the preeervation of speciea, breeding incaptivity. A foreststion •
•••••••-N.C••••I. Turk and Turk (1988) IV edn. inviro~ental Science. Saunderscollege Publishing N.Y.
2. Southwick V.H. (1976), 8cology and the quality of environ.entZedn. Van Noetrand N.Y.3. Sax N Irving : Industrial pollution, Van Nostrand ReinholdCoJIpanyN.Y •
•• Meleon L. He-erow Indu.trial water pollutioncharacter.tics and treat.ent. Addison weeley publishing origins,
co,pany.5. Trivedi N.!., Goei P.K.,
Ecology and Environ.entalKarad.
Triaal C.L.: PracticalScience. Environ.ental
!llethodll inPublishen.
6. Bedi: Social and preventive .edicine.
1. Benton A.H., Warner 11.8. (1958) : Principles of field biology andecology. Mc.GraN Hill.N.Y.8. Odu. S.P. f1911}
Saunder8 Co. Funduentala of Ecology 3rd Sdn. Ii.B.
9. Needhu and Heedhaa - A guide to the study of freshwater biology.10. Welch (1952) Llanology
,
11. Tonapi (1&80) Freshwater ani.ala of India.12. C.S.Ran (1995) Environaental Pollution Control En.lneering Wiley
Eastern Publication,
13. S.S.Oara : A Text Book of Environaental Chs.letry and Pollution(1991) control. a.Chand and eo.pany, New Delhi, India •
.w ChordateL
2.
3.
t.
5 •
r~l." L
Origin and Phylogeny of CbordateConcept of adaptive radiation and application ot Chordate - fish(Elaa~branchii. Ceteihtbyee, A.phibia, Reptile. Avee, Maa.alel.Study of Endoekeleton and Joints in any Duttable .~l.,
"Co.parative anato~ of autono~u8 nervous sytee&.eosparstive anatollY of sense organs - ear and eye.
ZOO 103 (b) Bioat.ti.tieaIntroduction to Statistics -1.1 Meaning of Statistics. '~
1.2 Its UseR in Biological Sciences,2. Collection of Statistical Datil.-
2.1 Concept of population, Sa.ple, paraaeter, statistics pri.aryand secondary data.
2.2 Sa.pling procedures, Purpose ot saapling.Sa.pling .etbodasa.pling, Syste.aticexpected) •
3. Classification -3.1 Meaning, objects of classification.
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CO.putations.etbod using
,
Mean, Median,for groupedfor.n1ae)
Class, Class li.its, Class boundrles, Mid points, classinterval, Frequency, CUIUlative frequency.
t' c","lative frequency distributione.Frequency d1stribu Ions, ~ ~
t.'
MelUlures of Central Tendency and Dispersion.4.1 Concept oC Central Tendency and Despersion.
Standard deviation, Definl~ions,and ungrouped data. (by- dIrect
3.3
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4.3 Coefficient at variation DetinatioR, eo.Putation and itaU8es.
5. Correlation IlIldRerreadaD
••,
5.1 Meaning of correlation and tyPes of correlation Karlperson'. CoeffiCient of Correlatioll. Detination,Interpretation. Co.PUtstion tor grouped and ungrouPed data.
5.2 Meaningof r!!greaainn,8tate~nt of !'egre"ion "Quationa,for t••.o varja, regression coefficient, co.putatiohs ofRegreaa10n eqUation tI'Oll ra", data and So.lIarieed data,Relation bet"'een RegreSsion Coefficient IlIld Cor~lationCoefficient.
6. Teating of HYPOthesis:
Alternativestatistical
Meaning of HyPotheSis, Null HyPotheSis,HyPotheSis, finding of at4ndard values fro. thetable.
6.2 Teat baaed on Noraal diatribution (two tailed testa)Large a"ple teata for testing ~llll,Difference of ~ena,Proportion end difference between the proPOrtiona.
6.3 Teat baaed on distribution, Testing tbe ~en, Difterence inthe aeens, hired t-teat.,6.4 Teet besed on X (Chi~SQuereJ distribution. Teat of goodneaaof tit, Test of indePendence of ettributea.
6.5 Teat baaed on F - distribution for testing the Variances.6.6 Si'ple NU~ricel Prable•••
7.1Concept end Meaning ot desirn of eXPeri.-ents.RandqhationReplication, Local Control. ,
1.2 Brief and ele.entery eXPIenation of CHO end RBn.7.3 Nuaerlcal Proble••.
I)'I'I
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IeCst••••••CIl ~ .l9r. Chord,tABellairs - Reptiles (Hutchinson)Green - Anato.y of the Rat (Hafner):::::, Co.,~."•• '~".b,,". ",,,_ W'h."", " ChIo••
t••,~, ""'OOh, M11l~ Md''''100 _ 10hlh7<>',",("",JNew'an - The PhylliSChordete (H••.•.•llillen}
Noble ~ The BiOlogy of ASPhlbia (Dover}P"'., Md •••••" - 7"1 •••, 0' tool,,, .,' II ("o'
IIIMJ
B
10) ParlUljape The AnatollY of the Garden Lillzard (Caloteaveraicolor)(Boulenger) (University ot Poona)
11) Pra8ad - Vertebrate Zoology (Kitab Mah!!.l)12) Roaer - The Vertebrate Body13) Taylor and Weber - Functional M~alian Anatoay (Villi Nostrand)14) lIalla - The Vertebrate Eye and Its Adaptive Radiation (Hafner).15) Walter and Sayles.- Biology ot the Vertebrates (Mac.tIlan)16) Weichert - Anata.y ot Chordates.11) Young The Lire of Vertebrate" (Oxto~).18) Young - The Life of Ka..al (Oxford),
REPIRJNcE .f!Qll!W .ml. BIOSTATISTICI
1) Statistical Methode: G.II.Snedecorand W.e.Cochran.2) Vital Statistics: M. Orkin IlIId R. Drogio. o'J) Statistical Methods: II.S.Dixon and F.Massey,4} A Short Text Book of Medical Statistics: A Bradford Hill.5) Funda.entale of Bio.ell'Y : L.N.Balaaa.
fractic.lI .in Coune lQIl:ill 1Al I9oachordata1. Morphology of nuclei in Proto!oa - A-aeba, Paraaoecius, Opalina,
Vorticella, Euglena, Stentor etc. '.2. Study of Acnidaria - Structure of Beroe, Pleurobranchia.3. Minor Phylla - ClaSsification as per theory courae.4. Study of Paruitic and Sedentary Cruataceana. I:5. Study of Head appendages in Crustaceana.6. Study of larvae of Echinodersa.7.-9.Diaaection of Grasshopper - Digestive eyates. NerYOUB ayatea,
Preparations of Acid/Alkali at given ncrsality andstandardization. ,
'",arations of Buffer of give.npH uemg pH setebr. " . t.
f'lucose y ,",.ColOrl-erICDetersination of concentratlona 0
sethod. '1 tildEatisation of Tyrosine by Folln phen~ Be o .Detersinations of pka values of glYCIne.
, 'd by paper Chroutographl'.To separate asIno aci B . oelectric precipitation.IBolation of Casein fros .ilk by IS h't by As~niu. SulphateIsolation of egg albumen fro. egg w 1 e~~:~;:~:a~~~~:acid fros tb~ Lizardi~~ excreta.Esti.ation of Protehi",bYt:~~et(:~etic' anhydrate sethod)Esti.ation of C 0 eB
i' t, b KjeldhalFra:gaents ro e n. f Totai Nitrogen YQu titative deter.ination 0Me~od/soresson'8 Method.. in blood and Urine by Picric acidDeter.ination of CreatinIneMethod.
_". , to ZOO 102 !nvi~nt&l BioloiP" :Practical Corres~ ••••ln
Bsti.ation of root bio.ass. , , and 'b' of terrestrial planta byDeter.ination of Chlorophy auBing Acetone method. f o. freshwater 888ples.EsU.ation of 02.and CO2 r
,"'
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.,,.4. Estiaation of total alkalinity. carbonates hi-carbonates,
bar<1neBs.5. Detel'llination of phyllical properties of water such as pH,
la.perature, total solid•• (TS},Total dissolved solids (TOO),light penetration by using BBChi disc.
6. Qualitative analysis of Zooplankton of lotie ecosyate•.7. Qualitative analysis of Zooplankton••of lantte ecoBral••••8. Study'of ItOrphological,Physiological and protective adaptatloDII
of freshwater anis.le with suitable exaaplea.9. Study of ~rphologic81. Physiological and protective adaptations
of terrestrial anlsale with suitable ~aaple8.10. Study of instrull('nts used in study of Environ.ental Biology
•a.. Measureeent of dust fall. '.13, Field trip to study pond/lake $B an exuple of lentic fresh wster
ecoeyste.,14. Field trip to study river ss exaaple of lotic freshwaterecosyetes,
15. Field trip to study .terrestrial ecosyste. and pollution affected&rea.
11, Biological usus.ent of water pollution (any three)a) Diversity index,bl Kothe's species deficit indexcl Odu. indexd) Sequential co.parison index (SCll
Reptiles up to order - suborderof Ave. up to order - suborder -
Cl88sification of Pisces/fishes up to order,Elaaaobranchi, Csteichtyes, DipnoiClassification of Aaphihia up to orderUrodela, ApOlia.Claasification ofDiapside.Classification!leognethae.Claaaification Of.888 1Metatheria, Entheris. s s up to order - suborderStudy of Axial skeleton f R bbStudy of Appendicul ~ a it/any auitable .u.al,Dieeection of Rat/::;yS:~7~0~lof Rabbit/any suitable 888ul.nerves 1 a ••vertebrate to study criainalDissection of Rat/an .nerves aut",.!" '" Y SUitable vertebrate to stud,S ~ vails8yete8 crieinaltudy of eye .uBcles in any suitable vertebrate.
Prat;ticalcorrUnqpdin' ~ lQQ 'M ill.••••• Blo.tati.tlc.Fo~ation of Frequenc dlst .Tendency snd Disnar,! Y ributlon and Co.putation ofD. - 00. centrslrawIng a rando. 1!lu.bertable. sup e fro. the population
Co.putation of Regreesion iDeterllinationof R . equat On8.Teat of Sign'fi egreSSIon equations.Test of Siln~f.cance (Large suple teats)Ana.lY8isof ~c::~\(S.all ~uple te5ts)ED and ltS interpretation.
"2)
"4',,6'7)""10)
"2)
"4'5)5)7)
In
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, ••
w m ill Gmleral Pbuiolou
'"teJIerllture
I
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•ZOO 201 (b) Writing 8cientific reportsoral presentation in English.
ExcretionRespiration:Circulation:
Physiology of excretion v,r.t. desert ani.als.Blood pig.ent H~globin and its aetabolia..Laws of Hae.oliynllllicB,Blood coagulation. ECG Normaland abnQr.al, ito significance.
MUBcle Contraction: Types aUBele contractionstwitch, iso.stric and isotonic contraction.Te.perature relations Adaptationspoikilothe~B and ther.al accli.ati~ation.Pineal gland structure and fonction.Physiology of Tactile, Gustatory and olfactory receptors.Electro Encephalogra. (EEG) Its significance sleep, MeMOry.Imaune Systea in Hu.an.
4.5.5.7 •a •9.
1.2 •,.
1. eoapilation of Experi.ental Record :Writing helps you reae.ber, observe and think,Writing an account of an expert.ent during ita progress,Writing a progress report.
2. Co••unicationCo.llunication
Internal reports,as part of science.
letters lIeltOranda
3. HOI<Scientiatll should IIrite :Explanation, e1Brity, cOllpletenellll, i.partiality, order,accuracy, objectiyity, at-plicity, appropriateness, balance,brevity, conaistancy, control interest, perauaeiveness,precision, sincerity, unity,How to write instructionsUnscientific writing exallplea
4 • Pregrulle ofThinking andof paragraph
IIriting :planningIIriting,
into••••ation,revising.
ideas, topic, outline, order
5 • Use ot Vocabulary :Meaning at warda, precise usage, tautology, synonYllS, unneces8aryqualification of word, .•••biguity.Technical terlls, nOlleuclature.Context, superfluous words, eireu.locution, reason tor verbosity.
6. Use ot good English :Noun, pronoun, verb, adverb, adjective, conjuction, articles,tenses, spelling etc,
7. Helping the reader :Decide what the reader needa to know.Write for eaay reading (hollto begin, conttol, explain, sentencelength, rhythll, style)Capture snd hold reader's interest,Obstacles to effective communication.
e, NUllbere contribute to precisionThe use of nu.bers.The use of tables.The Use of graphs and diagralls.
u
9. The &rt of illustrations, Ink drawing, photography.Writing the legend or caption.eo.pleted illustration.
10. Reading:How to read, .aking notes &~you read, writing & book review,
11. The part of a research report:Introduction, M/MResults, Discussion, Suuary, Acknowledge.ent,Reference.
12. The part of thesis and project repor18Preparing the .anuscript.Preparing the type8cript.Preparing the index.Preparing the typescript for the printer.
13. Editing and correcting:
14. TechniQ.ueof Oral PresentationPreparation of 8u••• ry note8, ehoice and U8e of Il.Udlovisualtechniques. Voice, pronounciation, punctuation, articulation,speed of delivery. Justification, interest, brevity, directness,eeQu<mceI!llJlhasis, citations, tautology.The t1••• factor.Hypothesis/analysis IUld synthesis,Questions IUld answers Listening, attentiveness,circudocation,
llXI..&l!1 ill &CODQlie ZooIo"Study of the following topics in relation to their econolicilportlUlce
•
Protozoa: i) Parasitic protozoa - E. histolytica, Trypanosola,Leishaania and Malarial parll.8ite.
II} Soil protozoaagriculture.
P8entoconas, Euglena their role in
Porifera: Sponge culture and its ilportance in industry and COllerce.Coelenterata : CorsI reefs as barriers their industrial andledicinal uses.
zoo 201 (b) Writing Scientif Reports and Oral Presentation in english.
1. Scientist MustWrite, R.NarraSB, (19711).Chapmanand Hall, Johnwiley and Sons, N.Y,
Z. Writting Scientific Papers in English (1975), MaeveO'Connor andF.Peter Woodford. Elsevier, Excerpta Medica. North-Holland.
3. The art of plain talk, Flesch, R.F. {196Z1, Collier - Macmillan,London, N.Y.
4. Guide for preparation of "cientific paper for publicaiton andGuide for preparation of author'a Abstract for publication,UNESCO(l9681 SC/MlJ/5,Vsllins, G.H. (1964) GoodEnglish; HOIl towd te it.
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5. Grogan D.J., Science and Technology; An introduction to theliterature 2nd ed., Bingley Publication London.
6. EI".ente of English GruIIUI.r.7. Oxford &ridWebster'lI English Dictionaries.8. English in Basic Medical Science, by Joan Maclean (1918) Oxford
Uni, Press.9. English 10 Education, by Elizabeth Laird (1977), Oxford Dolv.
Press.
References Book - ZOO201 (bl Economic Zoology.
1. Econollic Zoology by Srivastava, Co•••ercial Publication.berne, N"e..-DeIhL
Z, EconOllic Zoology by Fred V. Theobald3. £00000110 Zoology by ViahwlIpr" ••! K.K.
Akashdeep Publishing House, New Delhi4. Parasitology - by K.D.Chatarjee5. Parllai tology - by Kudo6. Useful lind Destructive Insects by - Hetrall and TenDt.7. HYllan Series - COllman Birds - Salim Ali8. Indian Birds - SaH. Ali9, Indian Snakes - Dr. Deans10, Fishes of India - Dry raucie11. Zo<.>!ogyPhylull eeries - KotpaI R.L.12. Life of JIlulllnls - Young.
ZOO202 - Cellular and Develop.ent Biology
transdi ff eren tiation,
cell hybridization,
I) Plasma lIlelllbrane- Structure, chemical nature, llodelB, passive andactive transport, junctions, changes in lIembrane due tofertilizatiolJ.
2] Chloroplast - Ultra structure and functions3} Lysosome- Ultra structure and functions,4) Mltochondri" - Ultra structure and enzymeswith fum:tionll.5) Ribosolles - Structure and functions in brief.6} Cell c:"cle Methods of analysis and regulation. Control of
cleavage pattern during developlllent.7) Mitosis and Meiosis - Mitotic apparatus, centrioles, Synllptonemal
,;ollple~.8} Concept of Growth - At cellular and organ level.Sl Cellular differentiati,m - Concept, totipotency, genic control of
differentiation.10) Eye lIorphogenesis.1I ) Tnnsf ou_at ion, transdeterllinat ion and
Concog'enes.12) Lillb d".eloplient and lillb regeneration.13} "uc1eo.;ytoplaSlllic interactions, somatic
'-';0 '." n s" l1r,1. lntl'od"ction t" QUlIlltjtati~'e Genetics,Le'!j~1,.,tJ., lc'lL(ic;[,.
l:,,;~~. \:. 1~7", -:-1,,,C,,;,,-,LJ(~ ,.f Bacterin and VirusesStn.Il", ttl Ea',ic Ge1\~tiGs and ~lo1"cul8.r Biolo~y,flln<:b"'-';.J, 'kie,,1'ifiC Puhlications, O)(ford.
Lewin, D. 1973, Gene Expression, Vol. 1 k 3, Wileyand 1978, interscience Publ.
•
9horrock, B 1978, The Genetics of Diversity, Holder andStoughton, London,
Strickberger, M.W. 1976 Genetics, Maeailian, New York.Whitehouse, H.L.K. TOI/BrdS an Understanding of the Mechanisa of
HeredityThe English language Book Society and Edward Arnold.
Practical N.r.t. ZOO 201 (a} General Ph,.iol0t71. To dete~ine the chlorides in the blood of hu.an and crab.2. Deter.illation or nitrogenous excretory products.
(Urine, sa-pIe of Cow, excreta of cockroach, li~zard, bird)3. To _<e •• anacle nerve preparation to record lIUecle twitch in
suitable ani.ale.4. Effect or tellperature on oxygen COnsD.ption in crab5. To ellU.ate the MOunt of chloride present in the urine of a
.a•••al (Cow).6. Effect of adrenaline on liver and .u8cle glycogen of Rat.7. Preparation of neuro-aotor end platen in any nuitable ani••l.B. Study of reflex action in any suitable aniaalB,9. Circulatory reaponse in Huaan skin.10. Quantitative estiaation of blood sugar level, before and after
innulin treataent.Pr~tlcal. of Zoo-202 : Cellular and Developaent.l Blolol1
1. Study of electrouicrographs of various cells orll'anelles2. Stud of principlee of phaee contrll.8t,electron.aicroscopy3. Study of .itcsis in onion root tip/Rhes polen4. Study of aeisois in Grasshopper teetes
(Special attention for prophase I stages)5. Preparation of ehro.oso.ee froa bone aarrow cells of Rat6. Detection of carbohyderates (aucopolynacchrides and glycogen)
by PAS reaction7. Detection of protein by BrollOphenol blue .ethod.8. Detection of DNA by Feulgan reaction9. Detection of .itochondria in tissue sections10. Detection of RNA by Methyl green pyronin aethod11. Deter.ination of chlorophyll pigaente
by acetone aethad12. Teaporary preparation of chick e.bryo13. Per.anent preparation of chick eabryo14. Study of different types of eggs15. Study of cleavage patterns in AaphioxuB, fish, snail, frog. bird
and _also16. Study of GlI.8trulaein A.bhioxus, fisb, frog, bird and ••••• 117. Study of cell death with suitable ••terisllB. Study of regeneration in hydra/planeri •.
Practical. of Z00-203 (a) Molecular Bioiogy and BiotechDology1. Study of various techniques and instru.ente used in Subcellular
fraction and colori.etry and Beer's law.2. Esliastion of lotsl protein content in different cell fractions
(Nuclear, Crude Mitochondrial) by Lowry's Method.
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3. Isolation of DNA/RNAfro. biological source ••4. fall.ation of DNA by Diphenyl~ine reaction fro. different cell
fractions5. Deter.inalion of RNAby Orcinol reaction in given cell fractions6. Deler.inalian of Ruorose/glycerol density gradient and
centrifugation.7. Lysosoll&1/Protase activity in liver homogenate and Bub-cellular
fractions9. Visit to a biotechnologylab/tissue culture lab.
Practieala of ZOO-203 (b) Genetics
L
,.3.,.
"-,V 5.
6.
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Study of external Ilorphology and sexual di~rphie. of Drosophilaaalanogaster - nornal l mutants.Study of ~nohybrid crOSS and ratio in D. a.lanogasterCalculation of gene frequency of PTC testing in the given bu.anpopulation and study of its pedgree.Study of sone sex linked characters of D. melanogaster - whiteeye I bar eye.
Study of genetic diversity in soge aorphological charactera of D.aalanogaster.
Calculation of gene frequency of A,B, 0 blood group in the givenhuaan population.To detect synaptic pairing of ehroaasoaal abberation in aeioticstages (Prophase-I) of Gasshopper/testes.
