EHEC SOLUTION Raw milk and raw milk cheese protocol
bioMérieux S.A.
69280 Marcy l’Etoile, France
Tél. : 33 (0)4 78 87 20 00
Fax : 33 (0)4 78 87 20 90
www.biomerieux.comwww.biomerieux-industry.com
Enriched sample
1 Detection 2 Confirmation
25 g or 25 ml
in 225 ml Buffered
Peptone Water
+ 10mg/l acriflavine
0.8 ml
STEC-stx/eae
18-24 hrs / 37 ± 1°C0.8 ml
VIDAS®
ESP2
38 min2 x 30 µl
1 to 5 typical colonies per agar
ORchromID
®
EHEC
18-24 hrs / 37 ± 1°C
CT-SMAC
If suspected E. coli O157:H7 18-24 hrs / 37 ± 1°C
chromID®
Coli
If suspected non-O157:H7 EHEC22-26 hrs / 37 ± 1°C
SLIDEX®
E. coli
Corresponding to targeted serogroup(s)
STEC-stx/eae
STEC-Top 6
+
+
CONFIRMATION
-
-
ABSENCE
ABSENCE
VIDAS®
ESP1
38 min 5 µl
VIDAS®
ESP1
38 min 10 µl
EHEC rangeREF 30229 VIDAS® UP E. coli Serogroups (ESPT) 60 testsREF 42043 BPW 6 x 225 ml REF 414153 GENE-UP® STEC-stx/eae 192 testsREF 414154 GENE-UP® STEC-Top 6 24 testsREF 42729 BPW 10 x 225 ml (Minibags) REF AEB610309L BPW 6 x 900 ml REF AEB910303/4 BPW 4 x 3 L (DILUBAG™) REF AEB910305/2 BPW 2 x 5 L (DILUBAG™) REF 413697 ChromID® EHEC agar 6 x 200 mlREF 413093 ChromID® EHEC agar 5 x 20 mlREF 42606 Cefixime-Tellurite Mixture (CT) 6 x 4 mlREF 42017 ChromID® Coli agar 6 x 200 mLREF 43391 SMAC CT agar 20 platesREF 417404 SLIDEX® E. coli O26 50 testsREF 417403 SLIDEX® E. coli O103 30 testsREF 417485 SLIDEX® E. coli O111 30 testsREF 417406 SLIDEX® E. coli O121 30 testsREF 417405 SLIDEX® E. coli O145 50 testsREF 417407 SLIDEX® E. coli O157 50 testsREF 421195 SLIDEX® E. coli O45 30 tests
EHEC rangeREF 30229 VIDAS® UP E. coli Serogroups (ESPT) 60 testsREF 42043 BPW 6 x 225 ml REF 414153 GENE-UP® STEC-stx/eae 192 testsREF 414154 GENE-UP® STEC-Top 6 24 testsREF 42729 BPW 10 x 225 ml (Minibags) REF AEB610309L BPW 6 x 900 ml REF AEB910303/4 BPW 4 x 3 L (DILUBAG™) REF AEB910305/2 BPW 2 x 5 L (DILUBAG™) REF 413697 ChromID® EHEC agar 6 x 200 mlREF 413093 ChromID® EHEC agar 5 x 20 mlREF 42606 Cefixime-Tellurite Mixture (CT) 6 x 4 mlREF 42017 ChromID® Coli agar 6 x 200 mLREF 43391 SMAC CT agar 20 platesREF 417404 SLIDEX® E. coli O26 50 testsREF 417403 SLIDEX® E. coli O103 30 testsREF 417485 SLIDEX® E. coli O111 30 testsREF 417406 SLIDEX® E. coli O121 30 testsREF 417405 SLIDEX® E. coli O145 50 testsREF 417407 SLIDEX® E. coli O157 50 testsREF 421195 SLIDEX® E. coli O45 30 tests
STEC-stx/eae
STEC-Top 6
+
+
CONFIRMATION
-
-
ABSENCE
ABSENCE
VIDAS®
ESP1
38 min 5 µl
VIDAS®
ESP1
38 min 10 µl
© 2
016
bioM
érie
ux, I
nc., •
Doc
umen
t and
/or p
ictur
es n
ot le
gally
bin
ding
. Mod
ifica
tions
by
bioM
érie
ux ca
n be
mad
e wi
thou
t prio
r not
ice •
The
blue
logo
, API
and
GEN
E-UP
are
use
d, p
endi
ng a
nd/o
r reg
ister
ed tr
adem
arks
bel
ongi
ng to
bi
oMér
ieux
, or o
ne o
f its
subs
idiar
ies,
or o
ne o
f its
com
pani
es.
ALO
A is
a tra
dem
ark
belo
ngin
g to
Bio
life S
.r.l.. A
ny o
ther
nam
e or
trad
emar
k is
the
prop
erty
of i
ts re
spec
tive
owne
r. Pr
inte
d in
USA
.
bioMérieux S.A.
69280 Marcy l’Etoile, France
Tél. : 33 (0)4 78 87 20 00
Fax : 33 (0)4 78 87 20 90
www.biomerieux.comwww.biomerieux-industry.com
bioMérieux Industry
595 Anglum Road
Hazelwood, MO 63042 • U.S.A.
Tel: (800) 634 7656
Fax: (314) 731 8678
bioMérieux Canada, Inc.
7815 Henri-Bourassa Boulevard West
St. Laurent (Québec) • Canada H4S 1P7
Tel: (1) 514 336 7321
Fax: (1) 514 807 0015
EHEC SOLUTION Raw beef and veal – 25 g and 50 to 375 g protocols / Produce - 200 g protocol
Enriched sample
1 Detection200 g
in 800 ml
Buffered
Peptone
Water
25 g in 225 ml
prewarmed
Buffered
Peptone Water
50 to 375 g
in 150 to 1125 ml
prewarmed
Buffered Peptone
Water
STEC-stx/eae
VIDAS®
ESP2
38 min2 x 30 µl
1 to 5 typical colonies per agar
ORchromID
®
EHEC
18-24 hrs / 37 ± 1°C
CT-SMAC
If suspected E. coli O157:H7 18-24 hrs / 37 ± 1°C
chromID®
Coli
If suspected non-O157:H7 EHEC22-26 hrs / 37 ± 1°C
SLIDEX®
E. coli
Corresponding to targeted serogroup(s)
10-24 hrs / 41.5 ± 1°C0.8 ml
8-24 hrs / 41.5 ± 1°C0.8 ml
10-24 hrs / 41.5 ± 1°C0.8 ml
0.8 ml
2 Confirmation
www.biomerieux-usa.com
