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Page 1: Eligible authors at German institutions may publish open ... · 3, 2020. A 4hour workshop in which Mr. Mark Newton will take you through his best practices in software implementation

56 13

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VOLUME 24MARCH

2020

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Page 2: Eligible authors at German institutions may publish open ... · 3, 2020. A 4hour workshop in which Mr. Mark Newton will take you through his best practices in software implementation

Your accepted OA article is then published under a Creative Commons license on Wiley Online Library.

Corresponding authors who submit their work from participating Projekt DEAL

institutions may:

• Publish research articles in Wiley gold OA journals without charge to authors

(beginning 2019)

• Publish OA in Wiley hybrid journals without an author fee (from 1st July 2019)

Projekt DEAL and Wiley have partnered to support institutions and researchers

to advance open research, drive discovery, and develop and spread knowledge.

• Eligible authors at participating German institutions may publish OA and benefit from

full reading access to Wiley’s journals

• Authors from Projekt DEAL institutions may publish primary research and review articles

OA in Wiley journals, and retain copyright of their works, at no charge to the author

• All Projekt DEAL institutions have access to read Wiley’s academic journals back

to the year 1997

19 - RM000125

Eligible authors at German institutions may publish open access (OA) and benefit from full reading access to Wiley’s journals

If you have questions on your OA publishing eligibility, reach out to us: [email protected]

For more information and to check eligibility, visit: bit.ly/DEALAuthor

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Another Year - So What’s New?For me it apparently becomes a tradition to write the first editorial of the year about things that have changed. Change is necessary, but only seldomly easy. So, please find below an update of things that have happened so far and a preview of things to come.

New Website - New Look and Feel

The last year has brought about quite a change for the G.I.T. Laboratory Jour­nal. For those who are subscribers to our newsletter, you will have noticed that the layout has changed and that the online presence has moved to a new home named analyticalscience.wiley.com. The new webpage unifies the re­sources of SeparationsNOW, Spectros­copyNOW, Imag ing & Micrcoscopy, Mi­croscopy & Analy sis, and G.I.T. Laboratory Journal. That way the users can find the information in one place, rather than having to scout several sites. If you haven’t looked at the site yet, please do so now and I am sure you will enjoy the new layout.

Less Issues - More Focus!

For the G.I.T. Laboratory Journal in print, we decided to publish 4 issues this year. We will have a stronger focus on the spe­cial topic of the magazine (Spectroscopy in this issue). The next focus topics are Chromatography and Separation, Labo­ratory IT & LIMS, and Laboratory Equip­ment. I am confident that this selection of

topics reflects what you as readers want to see in our publication.

Meet us at the Fair!

This year we will also be at selected con­ferences and trade fairs, starting with Pittcon, which will unfortunately be al­ready over when you get this issue. Due to the uncertainty introduced by the COV­ID­19 outbreak, I am somewhat hesistant to write about where our team will be present. We are monitoring the situation and hope the fairs will not be cancelled. I have planned to be at the Paperless Lab Academy. The rest of our schedule is not yet fixed, but we will keep you updated as to where you will be able to meet us in person.

Mini-series “Digital Laboratory”

During this year, we will also have a mini­series about Digitalization in the labora­tory. The emphasis will be put on things that are already on the market and things that have been implemented. However, to add a little spice, there will also be a few ‘science fiction’ scenarios. As always, we will offer a mix of authors from the indus­try and academia so you can get the most complete picture about what’s what in the field.

Is that all? - Not quite yet!

One last thing: We have created the Wiley Analytical Science Award. This award is

for new and innovative products and so­lutions. We will have a jury of experts from the field who will select a shortlist of all entries. Then, we hand this shortlist over to a very diverse and unbiased, as well as very critical jury, namely you, the readers. Once the shortlist has been se­lected, you will have the opportunity to cast your votes. Then the three winning entries in each category are presented with the award. The deadline for the sub­mission of your product or solution is April 30th, 2020. For all further informa­tions, including the web based submis­sion tool, please refer to this website: https://www.pro­4­pro.com/en/specials/was_en.html

Dr. Martin Graf-UtzmannEditor-in-ChiefG.I.T. Laboratory Journal

www.hahnemuehle.com

Protect what matters

Food & BeverageEnvironment Diagnostic

The OriginalFilter Papers since 1883

Meet us atAnalytica 2020

stand 323hall A2

Magazine

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The Bot on the Bench 24Cooperative Robotics and the Routine LabT. Teutenberg et al. Cobots in the Analytical Laboratory 26Useful Tool or Gadget?K. Thurow

Chemical Syntheses Molecular Electronics 28Building Organometallic Chains Molecule by Molecule A. Vladyka et al.

Mass Spectrometry European Proteomics Infrastructure Consortium – Providing Access 32A European Proteomics InitiativeM. O’Flaherty

Identification of the Fluorescent Photoproducts 36Analysis of Two Phenylureas by Photo-Induced Fluorescence (PIF) and GC-MSP. A. Diaw et al.

ProductprofileYMC: Innovative UHPLC/HPLC Solutions for BioLC 39

ShowcaseWITec: Find, Classify and Identify Microparticles 40

Products 40

Index/Imprint Inside Back Cover

Cover StoryMisa – A New, Portable Food Analysis System 12Protecting Consumers with the Latest in Food Testing Technology

SpectroscopySingle Cell Technologies in Modern Core Facilities 14Complementary Technologies Are Underpinning Single Cell ResearchD. Davies et al.

The Epigenome: Illuminated One Cell at a Time 16Using scATAC-Seq to Study the Impact of Epigenetic VariationK. Simonyi

UV-Vis and FTIR Instruments in Regulated Environments 18An Innovative Solution for Molecular SpectroscopyJ. Hesper

Multivariate and Multiway Calibrations 20What Analytical Chemists Would Ask From Aladin’s LampA. C. Olivieri

Laboratory Automation The Digital Laboratory 23A Short IntroductionM. Graf-Utzmann

EditorialAnother Year - So What´s New? 3M. Graf-Utzmann

Events Paperless Lab Academy 6

News 7

Read & WinGenomic Approaches in Earth and Environmental Sciences 9G. Dick

20 Minutes 10

Congratulation!

The winner of Read&Win issue 4/19 is T. Garrett from Newberry, Florida.

The winner of Read&Win issue 5/19 is S. Gunstrup from Denmark.

The next prize draw is on page 9

A human’s inherited DNA sequence remains relatively consistent across healthy cells throughout life. But over time, epigenetic modi-fications accrue and influence the way those genes are expressed and, in turn, how the cells function. The challenge with understanding how these changes impact human develop-ment and disease is that there is no single epi-genome to decode – it differs across individual cells. Below we discuss the technological ad-vances that are now allowing scientists to piece together the impact of epigenomic modifica-tions at a single-cell level and at scale.

More on page 16

Preview Issue 2/20Coming out 27th May, 2020

Special: Chromatography & SeparationTopics: Mass Spectrometry, Omics, Food

Magazine Articles Marketplace

Spectroscopy

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Contents

G.I.T. Laboratory Journal 1/2020

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Good Products deserve an Award –our Wiley Analytical Science Award.

Do not think twice, submit now!Where? was-award.com

Until when? 30th April 2020

Who? Every company whose product from the laboratory sector convinces with an innovative approach.

We are looking for the best products or solutions within the following categories:

A – SpectroscopyB – Lab AutomationC – SeparationD – MicroscopyE – Lab Equipment

Your Contact: Isabel Brenneisen [email protected]+49 6201 606 716

APPLYNOW!CLOSING DATE

APRIL 30, 2020

was-award.com

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WileyAnalytical

ScienceAward

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Foster Digital Revolution in your LABThe Paperless Lab Academy is ready to run its 8th European Edition, supported by more than 25 laboratory solutions and services providers.

Once again, the stage is set for discus­sions on strategies and implementations of 21st­century technologies for your lab­oratory and quality processes. The pro­gram is an agile combination of future horizon views ­ already running in some industries ­ and tangible real users’ cases on how to solve today’s concerns.

Along with keynotes speakers from dif­ferent industries sharing their digital transformation experiences, 15 interac­tive workshops and enjoyable networking sessions are planned.

A training opportunity on vendor selec­tion and risk management is also offered on the pre­congress day, Wednesday June 3, 2020. A 4­hour workshop in which Mr. Mark Newton will take you through his best practices in software implementation and as such in vendor selection manage­ment. Mark brings his 35 years of experi­ence in the pharmaceutical industry in QC Labs, computer systems validation and lab informatics at Eli Lilly. The Paperless Lab Academy, owned and organized by industry domain experts, is  the ide­al  learning platform for all companies that own a laboratory, involved in run­

ning, consolidating, integrating or simpli­fying scientific data management and laboratory processes.

»At the Paperless Lab Academy, We Challenge the Status Quo.«

Keynote speakers and solutions providers are very vocal about the fact that compa­nies should really consider investing in exploiting the quality data for perfor­mance evaluation, in stimulating process improvements and in simulating scenari­os that may lead to predictive quality.

From Keynotes to Interactive Work-shops, These Topics, Among Others, Will be Covered:

▪ Rapid transfer of raw data to a shared environment in the cloud allowing the immediate use of this data at all levels

▪ Easier integration of any laboratory in­struments through the Internet of Lab Things #IoLT to complete traceability of sample management and full data integrity compliance

▪ How Standard formats are simplifying the use and access to the data, even in GxP environments.

▪ Big Data being the new state of the evo­lution and Artificial Intelligence the real

driver to predictions, recommendations, classification and automatic recogni­tions.This new concept is destabilizing the organizations, not only IT depart­ments which were focused on process enablement but also laboratories and R&D departments that are trying to learn how to embrace this new concept.

▪ The use of structured and unstructured data in real­time which is quickly re­placing the use of old­fashion ap­proaches to evaluate and solve “after­the­fact” problems. This approach will allow the use of largely under­utilized data to predict rather than correct. It is also changing attitude at all company levels and real case studies will be pre­sented at the PLA2020.

▪ Broad ELN and LIMS implementation project

The Paperless Lab Academy 2020 is your place to be for answers relating to those topics and discussion with those who have already gone through it.

Everything you always wanted to know about Scientific Data Management, Labo­ratory Informatics, Paperless Processes and latest news from the industry.

Announcement

Registration https://www.paperlesslabacademy.com/registration

6 News

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Wiley Science SolutionsHelping laboratories build abetter future

sciencesolutions.wiley.com

Advantages of using a Wiley spectral library in thetoxicology field

Positively identifytrace compounds

Seamless integration Create efficient workflows

Complete both untargetedand targeted analysis

ISBN: 978-1-119-41223-6 ISBN: 978-3-527-34685-1 ISBN: 978-3-527-34327-0

Secrets of the Foot Many of us take our feet for granted, but they have a challenging job in the bio-mechanics department. When we push off with the ball of the foot, the force we apply exceeds our body weight, causing the middle of the foot to bend. Yet the foot maintains its shape be-cause it is stiff enough to withstand this force. Re-searchers have long de-bated what gives the mid-foot its stiffness. Now, a new study, published in Na-ture, has shed light on the importance of a little-studied structure called the transverse arch (TA), which runs across the foot. The researchers theorized that the TA contributes even more to this effect, much like how rolling a floppy piece of paper makes it harder to bend. Using a protocol developed with the help of computer simulations, and experi-ments on plastic and mechanical models, the researchers found that about half of the stiffness in human cadaveric feet is controlled by the TA.Original publication: https://bit.ly/GLJ0120-N1

Clever Pathway to Branched OlefinsHydroformylation, or oxo synthesis, is an industrial process for obtaining aldehydes from olefins. Current catalytic processes yield both linear aldehydes, which are key in-termediates for the detergent and polymer industry, and branched ones, which are

considered a powerful tool for the fine chemical industry because of their possible use in producing enantioen-riched products, that is, products fea-turing a greater proportion of a given enantiomer of a chiral substance. The researchers first screened several catalytic conditions to maximize the yield of the branched product that could be obtained with homogeneous catalysis. They then showed how they could go beyond this limit and achieve much higher branched selectivity by adding MOFs to the reaction mixture. They also tested different MOF topolo-gies to understand the role of the MOF environment in such a change in selectivity. The group was able to show that the mi-cropores of MOFs push the cobalt-catalyzed hydroformylation of olefins to kinetic re-gimes that favor high branched selectivity, without the use of any directing groups. The addition of MOFs allowed branched selectivity of up to 90% in these cases, a feat that cannot be achieved with existing catalysts.Original publication: https://bit.ly/GLJ0120-N2

New Subtype of SchizophreniaPenn Medicine researchers are the first to discover two distinct neuroanatomical subtypes of schizophrenia after analyzing the brain scans of over 300 patients. The first type showed lower widespread volumes of gray matter when compare to healthy controls, while the second type had volumes largely similar to normal brains. The findings, published Thursday in the journal Brain, suggest that, in the future, ac-

The longitudinal arch has been well studied when it comes to foot stiffness, but this research found that the transverse arch may be more important.

© O

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logies increase the density of the ole-fins while partially preventing the ad-sorption of the synthesis gas.

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Magazine

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G.I.T. Laboratory Journal 1/2020

counting for these differences could inform more personalized treatment options. Schizophrenia is a poorly understood mental disorder that typically presents with hallucinations, delusions, and other cognitive issues - though symptoms and re-sponses to treatment vary widely from patient to patient. Up until now, attempts to study the disease, by comparing healthy to diseased brains, has neglected to ac-count for this heterogeneity, which Davatzikos says has muddled research findings and undermined clinical care. Original publication: https://bit.ly/GLJ0120-N3

Gut Microbe Could Cause CancerCancer mutations can be caused by common gut bacteria carried by many people. This was demonstrated by researchers from the Hubrecht Institute (KNAW) and Princess Máxima Center in Utrecht, the Netherlands. By exposing cultured human mini-guts to a particular strain of Escherichia coli bacteria, they uncovered that these bacteria induce a unique pattern of mutations in the DNA of human cells. This mutation pattern was also found in the DNA of patients with colon cancer, implying that these mutations were induced by the ‘bad’ bacteria. It is the first time that researchers establish a direct link between the microbes inhabiting our bodies and the genetic alterations that drive can-cer development. This finding may pave the way to prevention of colorectal cancer by pursuing the eradication of harmful bacteria. This study may have direct implications for human health. Individuals may be screened for the presence of these genotoxic bacteria; it is reported that 10-20 percent of people can harbor the ‘bad’ version of E. coli in their intestines. Antibiotic treatment could eradicate these bacteria early on. In the future it may be possible to catch colorectal cancer development very early or to even prevent tumors from developing.Original publication: https://bit.ly/GLJ0120-N4

Believing in Conspiracy Theories Could Increase Non-Normative ActionsConspiracies abound in society and can have real world impacts when it leads some people to act, whether that means becoming more engaged politically, or less engaged. Previous research linking conspiracy beliefs and political actions provide mixed results. Some studies show people with a conspiracy worldview are more likely to disengage politically, while others show they are more engaged. The re-searchers conducted two experiments, one in Germany (194 people) and another with Mturk workers based in the United States (402 people). In both experiments, people were assigned to imagine being in a particular type of society. Some were assigned a conspiracy-focused description that suggested a few powerful groups controlled the fate of millions, others read an intermediate scenario where people wondered if the media and politicians could be trusted, and another group read about a world view that governments and the media were trustworthy and trans-parent. Each person was then asked a set of follow-up questions about what politi-cal actions they’d be willing to engage in, from “normative” actions like voting, participating in rallies, or contacting media or politicians, to “non-normative” ac-tions such as destroying property, harming others, or other illegal behaviors. In both studies they found people who were presented with a high conspiracy scenario were more likely to engage in the non-normative political actions than those pre-sented with a low conspiracy scenario. Political engagement for normative actions.

Was higher for those reading about low conspiracy scenarios compared to the other two groups.Original publication was not yet available at the time of printing. It is pub-lished in the Journal: Social Psychological and Personality Science

Quantum Entanglement

The CAS key lab of quantum information makes a significant progress in quantum ori-enteering. Researchers enhanced the performance of quantum orienteering with en-tangling measurements via photonic quantum walks. Thanks to quantum entangle-ment, quantum information processing is much more efficient than its classical counterpart in many tasks, like quantum computation, quantum communication, quan-tum metrology, and so on. Quantum entanglement can manifest itself in both quantum states and quantum measurements. On contrast to the extensive research of entan-gling states, there are few experimental studies of entangling measurements because entangling measurements are difficult to realize. Their work demonstrated a truly non-classical phenomenon that is owing to entanglement in quantum measurements in-stead of quantum states. Meanwhile, it offers an effective recipe to realizing entangling measurements in photonic systems. These results are of interest not only to founda-tional studies of quantum entanglement and quantum measurements, but also to many applications in quantum information processing. Original publication: https://bit.ly/GLJ0120-N5

Social Media and Vaccine Scepticism in ChinaIn July 2018, Chinese government inspectors determined that Changchun Changsheng Biotechnology, a prominent manufacturer of vaccines in China, had violated national regulations and standards when producing 250,000 rabies vaccine doses. The violation might have undermined the effectiveness of the involved vaccines. News began slowly escalating on Chinese social media platforms not long after the incident. Researchers have analysed the impact social media had on the debate. They warn about the dan-gers of public perception of even a single vaccine safety incident. The team also be-lieves the possible emergence of vaccine opposition in China is a potential cause for concern, especially considering the density of several large Chinese population centers.Original publication: https://bit.ly/GLJ0120-N6

Ultrasonic Attack on Siri and GoogleUltrasonic waves don’t make a sound, but they can still activate Siri on your cellphone and have it make calls, take images or read the contents of a text to a stranger. All without the phone owner’s knowledge. However, new research from Washington Uni-versity in St. Louis expands the scope of vulnerability that ultrasonic waves pose to cellphone security. These waves, the researchers found, can propagate through many solid surfaces to activate voice recognition systems and, with the addition of some cheap hardware, the person initiating the attack can also hear the phone’s response. The team suggested some defense mechanisms that could protect against such an attack. One idea would be the development of phone software that analyzes the re-ceived signal to discriminate between ultrasonic waves and genuine human voices. Changing the layout of mobile phones, such as the placement of the microphone, to dampen or suppress ultrasound waves could also stop a surfing attack. Their final hint is to put the telephone on a tablecloth to filter out the Ultrasonic waves.Original publication: http://bit.ly/GLJ0120-N7

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8 News

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G.I.T. Laboratory Journal 1/2020

Other book presentations: https://bit.ly/WAS-RNW

What is your main focus in research, what is your main scientific interest?The main focus of my research is on how microorganisms influ­ence their environment, ranging from questions about how and why they produce toxins that are a concern for the health of drinking water and ecosystems, to understanding the history of Earth’s oxygenation through the lens of microbial mats that are functionally analogous to those that were prevalent in deep geo­logical time. Much of my research draws on “omics” approaches – genomics, transcriptomics, proteomics – to track the activities of these microorganisms as they exist in communities in the en­vironment.

What was the reason to write the book?The field of environmental omics has emerged and advanced very quickly. The goal of this book was to provide a reference and a starting point for researchers who wish to get familiar with this field.

What is the target audience for the book?Microbiologists and geoscientists in academica, industry, and government agencies who want to learn about this rapidly­emerging field.

What knowledge is prerequisite for the book?Basic microbiology and/or earth and environmental sciences.

What is the structure of the book?The book is structured to first provide an overview of the appli­cations of omics­based approaches to the earth and environ­mental sciences, as well as background on the architecture of microbial genomes and the ecological and evolutionary forces that shape it. Then the book goes through the various omics ap­proaches, including metagenomic assembly and binning, func­tional annotation, metatranscriptomics and metaproteomics, and downstream approaches.

What is the - in your opinion - most surprising discovery?Geomicrobiology has provided many surprising discoveries in the past few decades. To me the most surprising is the stagger­ing diversity of microorganisms in the environment – most or­ganisms in the environment have not been cultured, and contain genes of unknown function. Thus there is a preponderance of microbial “dark matter” that is poorly understood, and plenty of room for discovery in the future!

Also available in electronic formats

Gregory J. Dick

Dick, G.Genomic Approaches in

Earth and Environmental SciencesSeries: Analytical Methods in

Earth and Environmental Science2018.

Hardcover.ISBN: 978-1-118-70824-8

Genomic Approaches in Earth and Environmental SciencesThe past 15 years have witnessed an explosion of DNA sequencing technologies that provide unprecedented insights into biology. Al-though this technological revolution has been driven by the biomedical sciences, it also offers extraordinary opportunities in the earth and environmental sciences. In particular, the application of “omics” methods (genomics, transcriptomics, proteomics) directly to environmental samples offers exciting new vistas of complex microbial communities and their roles in environmental and geochemical processes. Genomic Approaches in Earth and Environmental Sciences begins by covering the role of microorganisms in earth and environmental pro-cesses. It then goes on to discuss how omics approaches provide new windows into geobiological processes. It delves into the DNA se-quencing revolution and the impact that genomics has made on the geosciences. The book then discusses the methods used in the field, beginning with an overview of current technologies. After that it offers in-depth coverage of single cell genomics, metagenomics, metatran-scriptomics, metaproteomics, and functional approaches, before finishing up with an outlook on the future of the field.

is an Associate Professor in the Department of Earth and Environmental Sciences at the University of Michigan. He is a geomicrobiologist, mean-ing that he studies how organisms influence their environment. He current focus in on (i) production of toxins by cyanobacteria in lakes and (ii) under-standing controls on oxygen production in cyanobacterial mats.

Win

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Read & Win 9

Magazine

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G.I.T. Laboratory Journal 1/2020

© pict rider - Fotolia.com

Weird Science

Happy birthday to you and washing hands

☛ The Center for disease control in the US has published a guide that shows how to wash your hands

correctly. This may sound odd, but there are things that can and usually do go wrong. I, for one, did not

know that humming the “Happy Birthday” song twice while scrubbing the hands is a good timer for the

20 seconds needed for proper cleaning. Further details (including the science behind it all) can be found

under this link:

https://bit.ly/GLJ0120-WS1

Smile, you’re on camera!

☛ A team of scientists have deployed a large number of camera traps in the Bukit Barisan Selatan

National Park in Sumatra. They detected 39 species, including such endangered species as the Sumatran

tigers and Sunda pangolins. The original publication can be found here:

https://bit.ly/GLJ0120-WS2

Super urinating fish and the mangrove

☛ Researchers from the University of Michigan have tracked tropical fish in a mangrove estuary on the

Bahamas. Their analysis shows that the most active individuals of the grey and cubera snappers provided

disproportionally large amounts of Nitrogen (in the form of Ammonia). Subsequent simulations showed

that the Nitrogen introduced by the two snapper populations is roughly equivalent to all other Nitrogen

sources. The study was published in Science Advances.

https://bit.ly/GLJ0120-WS3

Web-Tip!Monty Hall and the GoatThere are probably not too many people out there that have not come across the so-called Monty Hall Problem. The simplified descrip-tion is the following: The player is presented with three doors, behind two of which there is a goat. Behind one door is the great prize: a car. After the first choice, the host of the ga-me-show opens one of the doors that were not chosen and presents the player with the choice to change the original choice or to stay with it. Which strategy is more likely to get the main prize? Here is a link to a simulating tool that lets you try and find out. Have fun with this one!

https://bit.ly/GLJ0120-MH

Read and Win!To have a chance of winning the book find the origi-nal figure in this issue from which the image below is taken. Send the title of the article to [email protected] with the subject Read & Win!

All correct answers will be entered in a prize draw and the lucky winner will receive a copy of “Ge-nomic Approaches in Earth and Environmental Sciences”, which is featured on page 9.

Closing date: May 6th, 2020

10 20 Minutes

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G.I.T. Laboratory Journal 1/2020

Look closely is the task in this puzzle. We manipulated the right image. Find all eight mistakes we have hidden for you. Send the image with the marked errors to [email protected] with subject line “Picture Puzzle”. Among the correct submissions, the lot decides the winner of a small sur-prise. Closing date is 6th of May, 2020. !Picture Puzzle

Sudoku! Fillomino!

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20 Minutes 11

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G.I.T. Laboratory Journal 1/2020

The Basics of SERS

SERS is a Raman spectroscopic technique that transforms moderately sensitive Ra­man spectroscopy into a trace analytic method. The discovery of SERS in 1977 was a serendipitous result from attempt­ing to observe Raman spectroscopy from monolayers of molecules adsorbed onto roughened silver electrode surfaces. To date this initial discovery has expanded to surfaces as variant as free floating

nanoparticles to sophisticated nano­ma­chined silicon structures. Regardless of the surface state of the material, SERS re­sults from special optical properties of sil­ver and gold and the size of the surface structures.

The SERS effect stems from a unique property of these particular metals that, unlike other materials, create a reso­nance with the electric field of the laser light and the Raman scattering. This res­onance creates large electric fields at the

metal’s surface that enhance Raman scattering by as much as 107. The elec­tric field enhancement is located only at the surface of the SERS active materials. This is an important limitation to the technique ­ the target material must be extremely close (< 3 nm) to the surface. Generally, nitrogen and sulfur containing compounds are the strongest materials to adsorb to these noble metals and ex­hibit the greatest SERS effect. Fortunate­ly nitrogen and sulfur containing com­pounds includes most food adulterants and most active pharmaceuticals (legal and illegal).

The SERS effect can be summarized by: ▪ Million plus fold enhancement of Ra­

man signals due to nanostructured surfaces.

▪ Selective adsorption of materials at these surface.

This combination creates a useful tech­nique of trace sensitivity for food contam­inants.

Misa – A New, Portable Food Analysis SystemProtecting Consumers with the Latest in Food Testing Technology

Industrial dyes used in brightly colored candies, pesticide residues on fruits and vegeta-bles, pharmaceuticals illegally added to herbal medicines - Contamination of food prod-ucts is a global problem, whether it is done intentionally for profit or accidentally through negligence, the end result is the same – consumers pay the price. The problem of food contaminations can be addressed with complex analytical laboratory techniques such as GC-MS and HPLC, but time, skill, and cost requirements limits their usage to the confines of well-equipped laboratories. Surface Enhanced Raman Scattering (SERS) – an extension of Raman spectroscopy - permits detection and identification of analytes in concentrations as low as parts per billion. Food analysis with SERS can be fast, con-venient, and inexpensive.

12 Advertisement

Cover Story

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Advantages of SERS over Tradi-tional Trace Analysis Technologies

Food samples are normally a complex matrix consisting of proteins, fats, starches, sugars, and many other materi­als. Contaminants – when presented, are usually at trace levels. Analytical tech­niques that carry the claim of “trace” de­tection inevitably involve separation of the analyte from an overwhelming ma­trix. Terms such as part per million (ppm) or part per billion (ppb) can be easily understood when related to popu­lations of people. A part per billion means that you can find a single person in a country of one billion. To a chemist this is a doable challenge – in less than an hour! But what does it require to make these measurements?

The key is to use features of the target analyte to distinguish it from its matrix. This concept of separation lays the foun­dation to differentiate various trace ana­lytical techniques and leads to the advan­tage of SERS.

Mass spectrometry is the most common trace analytical technique. The name mass spectrometry stems from its ability to identify materials through their molec­ular mass. For example, the fungicide Thiram has a molecular mass of 240.42. However, if a dilute sample of Thiram in a solvent is placed in a mass spectrometer, the more abundant solvent will hinder the detection of Thiram. As a result, mass spectrometric methods require a separa­tion method prior to the analysis. This is most often a chromatographic method such as gas chromatography (GC), liquid chromatography (LC), or Ion Mobility Spectrometry (IMS). These separation methods require time and skill to produce the separation. For example, GC requires a column of material that selectively ad­sorbs the matrix materials and passes the analyte. This can require up to 30 min­utes for a separation and detection. Fast­er separations are achievable with meth­ods like IMS, but then the detection can be very limited to a small class of materi­als.

SERS detection also involves separa­tion. But in this case the separation oc­curs much faster as the analyte adsorbs to the nanostructured surface. SERS can determine in seconds that a specific mate­rial is present. SERS identification of a molecular fingerprint is equivalent to the identification of a mass fingerprint and in the case of chemical isomers it is superior. SERS is an extremely attractive technique as it retains all of the appealing features of Raman – portable equipment, multi­analyte detection capabilities, and rapid analysis.

Misa to the Rescue!

Misa from Metrohm is a dedicated instru­ment for performing SERS measure­ments. It was developed with the focus for a simple, efficient and green testing solu­tion to address food safety threats. Easily swappable attachments allows flexibility of testing using different types of SERS materials. Metrohm also provides target­ed ID Kits that include test materials as well as detailed step­by­step instructions for measurement of common contami­nants in various food matrices. The advantages at a glance ▪ A mobile app provides simple, intui­

tive, guided workflows ▪ Automated analysis quickly and accu­

rately identifies trace contaminants ▪ Intelligent mobile platform enables re­

mote sharing of results, location, and hazard alerts

▪ Dedicated applications to inform sam­ple analysis methods

User Groups of Misa and Different Use Scenarios

With these advantageous in mind, Misa is an excellent mobile platform for the rapid identification of food contaminants at the point of sample.

Usage Scenario #1 – Mobile Food Inspection Laboratories

Food inspectors play an important role in securing the safety of consumers. At every point along a food supply chain, there is potential for fraud and contamination. The ability to detect harmful contami­nants and additives immediately means that no time is lost before vital informa­tion about the substance can be shared

and immediate steps can be taken by public authorities to protect consumers.

Usage Scenario #2 – Inspection of Incoming Raw Materials and/or Finished Products

At a food processing facility, raw ingredi­ents e.g. agricultural produce, may be sourced from several different farms. The ability to quickly verify that the incoming materials are free of contaminants or adulterants can reduce factory down time and wastage. Similarly, products can be quickly inspected at­line during manufac­turing to ensure no accidental introduc­tion of contamination. In both of these situations, rapid and efficient detection technology like Misa can lead to signifi­cant cost savings for the business.

Usage Scenario #3 – Preliminary Analysis in a Traditional Laboratory

While Misa cannot replace a “gold stan­dard” analysis instrument like GC­MS in a well­equipped lab, it can work in tandem with these traditional analysis technique to speed up laboratory work­flow. A posi­tive presumptive result using the Misa can help guide the laboratory scientists to configure settings on more resource in­tensive instruments to specifically con­firm analytes of interest, cutting down on analysis time, reducing wastage of re­agents and saving on valuable resources.

ContactDr. Wei YuMetrohm [email protected]

Advertisement 13

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The explosion of information that can be derived for specifically defined cells has great possibilities in biomarker discovery, therapeutic intervention and personalised medicine.

But to determine the function, genotype, and phenotype of an individual cell, it is essential to integrate several technological approaches. No one approach will fit all as it may depend on whether simple single cell information is needed (suspension­based methodologies) or whether there also needs to be a relational aspect i.e. the context of the cells in a tissue (tissue­ or slide­based methodologies). This allows to gain a greater understanding of the com­plex nature of cells reactions to stimuli, cell to cell interactions and cell fate.

Cells are still identified based on some a priori knowledge of the cell system and its response to, for example, drug treat­ment. True discovery needs a high throughput mode that is not present in most of the single cell technologies dis­cussed here. There are a number of tech­niques available and the one used will de­pend on the question asked and, more and more, researchers rely on integrated results from several approaches.

Suspension Technologies

Flow cytometry is a well­established tech­nique that uses fluorescence (labelled anti­bodies, fluorescent probes or proteins) to enable researchers to specifically identify sub­populations within a heterogeneous population. It has been established for al­most 50 years and has been embraced particularly by the field of immunology. The ability to measure structural parts of a cell (protein, DNA, RNA) and cell function (pH, calcium flux, apoptosis) on a cell­by­cell basis makes it an extremely powerful technology. Additionally, any sub­popula­tion identified by its fluorescence charac­teristics may be retrieved using a cell sort­er. Sorted cells may be passaged,

transplanted, put into functional assays or may be subject to downstream genetic, proteomic or metabolomic analysis.

Flow cytometry is still considered to be the gold standard of high parameter single cell analysis technologies [1]. Throughput is high – 10k/second is relatively easy to achieve, but can be high cost given the amount of reagents needed. Currently, it is not easily possible to examine more than 30 parameters per cell which can be limit­ing and challenging in looking at highly heterogeneous populations. Cells have tra­ditionally been sorted using electrostatic drop deflection but given the high pres­sures often used, low pressure microfluid­ics system such as the Miltenyi Biotec Tyto or the Cytonome microfluidic sorter can have advantages where subsequent func­tional studies are dependent on cells being as little stressed as possible. Single cell sort­ing for subsequent genomic, transcriptomic or proteomic analysis is also possible using an electrostatic drop deflection cell sorter.

Recently suspension mass cytometry (Fluidigm Helios) which uses metal­tagged antibodies to identify cells of inter­est has emerged. Although throughput here is slower, the number of analytes is increased compared with flow cytometry so allowing even greater sub­divisions of heterogenous populations.

Also, in recent years, imaging flow cy­tometry has allowed the positional infor­mation of fluorescence and the shape of cells to be captured as well as whole cell

fluorescence. This is especially useful when looking for translocation or locali­sation of fluorescence, and because it is flow­based also allows thousands of cells per second to be analysed making it suit­able for rare cell analysis.

Tissue Based Assays

Most single cell suspension approaches do not reveal spatial information either at the subcellular level or between cells as they need to be disaggregated. The Laser Scan­ning Cytometer was introduced in the late 1990s as a way of deriving subcellular in­formation from single cells on a substrate, and recent iterations of this technique has been used to analysis tissue sections but have a limited number of targets. To ad­dress tissue cytometry needs, in recent years there has been an expansion of new techniques each with their own advantag­es, that are compatible with standard tissue sectioning techniques i.e. cryopreserved or formalin fixed paraffin embedded (FFPE), material which is critical for their adoption.

Nanostring Digital Spatial Profiling (DSP) can analysis tissue sections using a panel of either antibody or oligonucleotide probes that are marked with unique photo­cleav­able oligonucleotide identifiers. Regions of interest down to a single cell are targeted with UV light releasing identifying oligonu­cleotides, which are collected and identified using either a multi­coloured fluorescent bar code system (up to 800 plex) or next generation sequencing (NGS) (kilo plex).

The Akoya Codex system can analysis tissue sections using a panel of antibodies that are labelled with an oligo duplex with unique 5’ overhangs. Antibody identity can be determined using iterations of single base extension to sequential identify oligo duplexes from the panel. Three antibodies can be identified per iteration by the addi­tion of fluorescent nucleotides and their

Single Cell Technologies in Modern Core FacilitiesComplementary Technologies Are Underpinning Single Cell Research

▪ Derek Davies1, Grant Calder2, Peter O’Toole2

Being able to make measurements on a single cell level allows us to link genotype, phe-notype and function. Several single cell technologies exist and there is now more of a need to integrate several of these into an analysis workflow. The complementary nature of flow cytometry, single cell sequencing and genomics platforms allows researchers to make more measurements than ever before. However, to do this knowledge of the ad-vantages and disadvantages of each approach is vital especially as there is, of neces-sity, more of a link-up between these technological approaches.

Fig. 1: Looking at cell division using the Amnis ImageStream. Using a combination of Propidium iodide staining (for DNA; red) and mpm2 staining (for mitotic cells; green), it is possible to identify cells in prophase (upper panel), metaphase (middle panel) and anaphase (lower panel)

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location within the section captured using standard fluorescence microscopy. Fluo­rescent dye molecules are then removed before the next iteration of extension and labelling. Antibody identity is determined by the iteration number and colour.

There are also techniques where the se­quential labelling of tissue sections with fluorescent tagged antibodies (3 to 5 dif­ferent colors) is performed in a single it­eration. Images are captured using stan­dard fluorescence microscopy followed by the removal or deactivation by photo­bleaching of fluorescence tags before the next labelling iteration. Zellkraftwerk ChipCytometry mounts a sample in a spe­cial flow cell that allows the exchange of solutions and Miltenyi have recently launched the MACSima system.

Hyperion mass cytometry system (Fluid­igm) can be used to analyse sections with a panel of up to 40 different antibodies each coupled with a unique metal tag that are not common in nature giving very low background. A scanning laser beam is used to ablate the sample causing the release of particles that are carried by a stream of in­ert gas into a mass spectrometer for identi­fication. This technique is capable of sub­cellular resolution down to 1 µm.

With Nanostring technology, conceptual­ly thousands of different proteins or RNA can be labelled simultaneously, but not with subcellular detail. Whereas both Co­dex and ChipCytometry approaches can yield subcellular information, their iterative approaches requires significantly longer processing time, limiting the total number of targets and are dependent on image analysis software to identify cells and their labelling signatures. Tissue­based mass cy­tometry (Hyperion) is less susceptible to background noise but requires specialized equipment and currently is limited in the total number of targets. In many tissue­based techniques there is a need to identify

individual cells using algorithms to sepa­rate cellular information from background allowing segmentation of single cells.

The Future

There is always a compromise – ideally, phenotype, function and positional infor­mation are wanted. No one technique does all of that to the same level of infor­mation/resolution, so prioritization may be necessary depending on the biological question being asked.

The challenges in the era of high­dimen­sional techniques are in data analysis as directed analysis is no longer possible. More and more operational pipelines have been published in general involving pre­processing of samples to remove unwanted and/or spurious events, this will be differ­

ent with each methodology. Data analysis of highly multiplexed samples is a chal­lenge and there is an increasing involve­ment of clustering or dimensionality reduc­tion algorithms used in flow, imaging and mass cytometry data, although this has been common in sequencing for some time.

However, it is clear that emerging tech­nologies such as those described here will be the driver in addressing tissue hetero­geneity in health and disease.

Affiliations1The Francis Crick Institute, London UK2Bioscience Technology Facility, Depart­ment of Biology, University of York, UK

ContactDr. Peter O’TooleDirector of the Bioscience Technology FacilityUniversity of YorkYork, [email protected]

More on Flow cytometry: https://bit.ly/WAS-Flow-Cyt

References: http://bit.ly/GLJ-Davis

Fig. 3: Dimensionality reduction of a 35-metal panel using tSNE within the Phenograph package.

Fig. 2: Data generated using the Hyperion Imaging mass cytometry system Human tonsil was stained with 35 differently labelled antibodies. False-color ima-ging of 4 of these is shown. Smooth muscle actin (red), CD31 (green), DNA (Blue) and CD45RA (Cyan). Left panel is slide overview, middle panel is zoomed in and right panel is after cell segmentation using Ilastik and Cell Profiler – this then allows quantitation of signal at the single cell level.

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Understanding how epigenetic mecha­nisms drive cellular differentiation and disease requires a nuanced map of the epigenome. Various modifications can al­ter the epigenome, such as histone modi­fication, methylation, nucleosome posi­tioning, proximity to other co­regulated genomic regions, higher order chromatin structure, and nuclear positioning. Until recently, the study of epigenomics relied heavily [1] on assays that focused on spe­cific areas of the genome, as well as bulk assays, which capture an average of the total modifications in large groups of cells. However, because every cell in our body has a unique epigenome that chang­es over time, the narrow scope and reso­lution of these assays makes them unsuit­able to make a detailed map of the epigenome.

Now, new developments in single­cell technology, namely single­cell assay for transposase accessible chromatin (scATAC­seq), have enabled scientists to map the epigenomes of entire tissues and organs at a single cell level. Their re­search is cataloging how different cell

types come together to form functioning organs and is uncovering new cell types along the way.

The Link Between Cell Function and Epigenetics

The presence or absence of a gene isn’t the only factor dictating whether or not a gene expressed. When DNA is tightly packed in the form of chromatin, the genes within are under or not expressed. Epigenetic modifications are what dictate the level of chromatin packing, rendering genes accessible or inaccessible to the cell’s transcription machinery. This, in turn, impacts cell behavior and function (phenotype).

At times, epigenetic modifications de­crease how much a gene is expressed by blocking transcription machinery. They can also amplify expression so that a cell is producing much more of that given gene than neighboring cells. The unique mosaic of epigenetic modifications within a cell helps regulate how much of every gene gets transcribed into RNA. Once

transcribed, that RNA can either be trans­lated into a protein or serve an enzymatic or structural function within the cell.

Mapping the Epigenome With scATAC-Seq

From cell to cell, there are enormous epi­genetic differences that evolve over time. We need single cell tools to study this het­erogeneity; to understand why popula­tions of cells are different, and how they function in the context of an entire organ­ism. Ideal for this task, scATAC­seq en­ables scientists to examine individual cells within large populations simultane­ously. The goal: build a complete map of the epigenome. With such a map, scien­tists could chart the epigenetic features of specific cell types throughout develop­ment. They could create a catalog of pathogenic epigenetic markers associated with disease. But to understand how scATAC­seq works and how it could be used to generate such a “cell atlas,” we must first understand its parent method, ATAC­seq.

ATAC­seq was not designed to provide single cell information. Instead, it offers a snapshot of the average epigenetic land­scape of hundreds to thousands of cells. Research published by Jason Buenrostro in 2013 in Nature Methods [2], describes the development of ATAC­seq. Compared to other common epigenetic methods – such as MNase­seq, DNase­seq, and ChIP­seq – ATAC­seq requires fewer cells per

The Epigenome: Illuminated One Cell at a TimeUsing scATAC-Seq to Study the Impact of Epigenetic Variation

▪ Kristopher Simonyi

A human’s inherited DNA sequence remains relatively consistent across healthy cells throughout life. But over time, epigenetic modifications accrue and influence the way those genes are expressed and, in turn, how the cells function. The challenge with un-derstanding how these changes impact human development and disease is that there is no single epigenome to decode – it differs across individual cells. Below we discuss the technological advances that are now allowing scientists to piece together the im-pact of epigenomic modifications at a single-cell level and at scale.

© Ja

ck M

oreh

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sample and can be completed in a single day. During ATAC­seq, a hyperactive transposase mutant known as Tn5 binds to open regions of genomic DNA, cutting the bound DNA and ligating NGS adapt­ers. After end polishing and PCR, ATAC fragments are sequenced to discern re­gions of open chromatin. The information derived from ATAC­seq indicates where in the genome transcription factors general­ly bind and where nucleosomes are typi­cally positioned. It gives a sense of the av­erage epigenetic state of a population of cells.

While useful in the lab, ATAC­seq does not have the single­cell resolution neces­sary to map the epigenome. Working out of Harvard, Buenrostro developed a tech­nique using microfluidic technology to segregate individual cells before conduct­ing ATAC­seq [3]. This resulted in the scATAC­seq method, published in Nature [4] in 2015. The microfluidic technique isolates hundreds of individual cell nuclei, then the Tn5 transposase tags open chro­matin regions with sequencing adapters. Next, the open chromatin library is ampli­fied with cell­identifying barcoded prim­ers. After amplification, discreet libraries – each representing a different cell – are pooled and sequenced to reveal open re­gions in the genomes of individual cells.

Profiles of the open chromatin regions of thousands of cells can be generated from a single scATAC­seq experiment. The results represent a range of epigene­tic variations throughout the population, which can be used to compare individual cells and classify different cell types with­in the sample. The results can identify epigenomic markers distinct to a specific cell type and can indicate that cell type’s relative frequency within the sample tis­sue.

Through scATAC­seq experimentation, scientists from numerous disciplines have uncovered new insights into complex dis­eases, such as autoimmune disorders, various cancers, and neurological disor­ders like Alzheimer’s, Huntington’s, schizophrenia, and Parkinson’s. But for a deeper understanding of epigenetic phe­nomena, researchers needed a way to evaluate more cells per experiment. In collaboration with Buenrostro, Ron Lebof­sky at Bio­Rad Laboratories and Caleb Lareau at Harvard University achieved this by developing two techniques [5] that expand on the original scATAC­seq meth­od. Driving both is a fundamental shift from the use of microfluidics to isolate

nuclei to a more scalable droplet­based technology (Fig. 1).

Their first new version of scATAC­seq (dscATAC­seq) was named for its use of droplet­based technology along with a single cell isolator to prepare a library of thousands of nuclei for sequencing. Each nucleus is held within an individual nano­liter­sized droplet. dscATAC­seq offers al­terations in the workflow, such as a cus­tom, hyperactive transposase, to improve signal resolution and enhance library complexity. The droplet­based workflow is also faster, simpler, and more scalable than the original microfluidic method. With these improvements, scientists get high­quality results while spending less time conducting each experiment. Taking advantage of dscATAC­seq’s expanded ca­pabilities to generate data for large num­bers of cells, researchers performed an unbiased analysis that cataloged the dif­ferent regulatory elements and cell types within an adult mouse brain.

Lebofsky and Lareau then went a step further by introducing combinatorial in­dexing to the dscATAC­seq workflow, cre­ating a new dsciATAC­seq method. In this approach, hyperactive mutant trans­posases integrate a first set of barcodes, into accessible chromatin regions as they are cleaved. These barcodes make it pos­sible to differentiate between cells, even when several are encapsulated in a single droplet. Because of this, a larger quantity

of cells can be processed in a given dsci­ATAC­seq experiment. During subsequent amplification, DNA fragments are tagged with a second set of barcodes. Using this combinatorial indexing approach, re­searchers can generate high­resolution chromatin accessibility profiles of as many as 50,000 cells per sample. Thanks to the colossal scope of the dsciATAC­seq assay, researchers have been able to study changes in the chromatin accessibility landscape in immune cell clusters across cell types at single­cell resolution. In one application, this depth of insight into the epigenome helped reveal the overall ef­fects of different stimulation conditions on human bone marrow­derived cells.

Conclusion

With an estimated 37.2 trillion cells mak­ing up the human body [6], tools with massive scalability are needed to gener­ate a high resolution cell atlas. Tools like scATAC­seq fulfill this need, equipping re­searchers to decipher the epigenome for a greater understanding of how biological systems work and how to best treat com­plex diseases.

ContactKristopher SimonyiGlobal Marketing Manager, Digital Biology GroupBio-Rad Laboratories Pleasanton, CA, USA [email protected]

Related Articles: http://bit.ly/WAS-Epigenetics

References: http://bit.ly/WAS-Simonyi

Fig. 1: For scATAC-seq, droplet-based technology is used to partition thousands of nuclei or whole cells into individual nanoliter-sized droplets to facilitate library preparation for ATAC sequencing.

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Data Integrity Compliance for Spectroscopy Systems

Compliance with data integrity require­ments is already a demanding issue for companies that require GxP compliance. In addition to LC and GC systems, regula­tory authorities have turned their atten­tion to spectroscopy systems such as UV and FTIR spectrophotometers. Just to mention a few: MHRA (Medicines and Healthcare products Regulatory Agency)[1], FDA (U.S. Food and Drug Administra­tion)[2] and WHO (World Health Organiza­tion)[3].

This increased attention to data integ­rity is based on repeatedly detected irreg­ularities – ranging from carelessness to deliberate manipulation with intent to de­ceive. Auditors expect active proof that no improper actions have been taken with regard to analysis results.

This approach corresponds to a policy that punishes any practice which even appears to be suspicious, and represents a break with previous approaches. When deficiencies are found during an FDA au­dit, they are not only reported to the com­pany being audited, but also published on the FDA website in so­called “warning let­ters”. The number of warning letters has

increased almost tenfold in the last 5­6 years.

So the question arises: what level of compliance is required to ensure the in­tegrity of data from spectroscopy systems?

Obstacles to Ensuring Data Integrity Compliance for Spectroscopy Systems

As illustrated in figure 1, compliance is based on Good Manufacturing Practice (GMP), which requires validation of sys­tems and analytical methods. Further­more, data, audit trail (metadata) and user operations must be time­stamped correctly in a secure environment. When a labora­tory has both chromatography and spec­troscopy systems, the elements shown in figure 1 apply to both such that compli­ance with data integrity requirements re­sults in the operations indicated in Table 1.

Typical systems retain audit trail data (metadata) within the spectroscopy data acquisition system as indicated in Table 1, but cannot manage spectroscopy and au­dit trail data in a unified manner. This means that data cannot be managed in a linked state. The same applies to the user management. Typical systems cannot manage both data management users and data acquisition users in a unified manner.

UV-Vis and FTIR Instruments In Regulated EnvironmentsAn Innovative Solution for Molecular Spectroscopy

▪ Johannes Hesper

A current issue of analytical data is often the lack of data integrity due to data modifica-tion and replacement. Regulatory authorities for analytical instruments are interested not only in chromatography systems such as liquid chromatographs (LC) and gas chromato-graphs (GC), but also spectroscopy systems such as UV-Vis and FTIR systems. Conse-quently, many analytical laboratories urgently consider how to ensure data integrity for spectroscopy systems in an efficient and economical way. Innovative software solutions combine different analytical instrument categories into one management system to sim-plify and to harmonize validation processes, GxP* data or even disaster recovery policies.*) GxP refers to all guidelines for “good working practice”, which are particularly impor-tant in medicine, pharmacy and the pharmaceutical chemistry.

© Jack Moreh, freerangestock.com

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Due to the attention on data integrity, focus has shifted towards provision of evidence to reviewers that no improper operations were performed with respect to analytical results. However, this ap­proach represents a policy of punishing any practice that appears suspicious, which is a major departure from the ap­proach used in previous investiga­tions[4]. This approach applies to both chromatography and spectroscopy sys­tems, meaning the conventional ap­proach cannot be used to ensure appro­priate compliance.

Advantages of a Unified Software Platform

Considering that data integrity compli­ance for spectroscopy systems could present a major obstacle for operating analytical laboratories in a regulated en­vironment, there is a need for an innova­

tive approach to ensuring data integrity that solves such problems.Reasons for one software platform: ▪ One software to learn ▪ One software to manage ▪ One software to update

Summary

Management of different instrumenta­tions like UV­Vis, FTIR, RF, ICP­MS, chro­matographic, or TOC equipment etc. with one common software platform enables addressing of compliance issues in a uni­vocal way, by having a single log. Harmo­nized procedures and immediate trace­ability of the data as well as a simple and quick consultation of the event register are highly valuable during inspections or audits. Finally, one software solution is easier to administrate, maintain by IT­de­partment and handle by operators.

ContactJohannes HesperShimadzu Europa [email protected]

References: https://bit.ly/WAS-Hesper

Fig. 1: GMP - data integrity requirements

Typical Innovation

Equipment Chromatography Spectroscopy Chromatography and Spectroscopy

Data Acquisition Yes - Yes Yes

Data Management Yes Yes - Yes

Audit Trail Yes - Yes Yes

User Yes - Yes Yes

Security Yes Yes Yes Yes

Time Stamp Yes Yes Yes Yes

Remarks 3x Systems to manage 1x System to manage

Tab. 1: Typical data integrity in laboratories having chromatography and spectroscopy equipment like FTIR, UV-Vis or RF next to LC or GC systems.

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DuraChill®

Changing the world of Chillers...again.

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Spectroscopy 19

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G.I.T. Laboratory Journal 1/2020

Classical analysis is a straightforward two­step process: (1) calibration using pure analyte standards, which renders the calibration model (slope and intercept of a calibration line), followed by (2) ana­lyte quantitation in unknown samples, in­terpolating its signal in the calibration line. This classical (univariate) approach requires that the analyte is the only sub­stance producing signal, or that: (1) all in­terferences are removed prior to the anal­ysis (extraction, masking, distillation), (2) the analyte reacts specifically so that only its product produces signal, (3) the ana­lyte and interferences are physically sepa­rated (chromatography), etc.

What Is Multivariate Calibration?

In comparison with this approach, multi­variate calibration may seem like a mythi­

cal animal. When building calibration models with spectra, such as near infra­red (NIR) or Raman, there is no need to separate analytes from interferences, and measurements can be done without dis­solving or grinding the sample. Just point­ing a handheld NIR spectrometer the size of a smartphone at a sample may provide useful analytical results in a matter of sec­onds. Examples that are today routine in most industrial laboratories or field activ­ities may appear as chapters of a science fiction book: fat, protein, moisture and starch can be measured directly on intact oil seeds, simultaneously, instantaneously and without organic solvents, organolep­tic properties of foodstuffs (wine, coffee, beer, meat, olive oil) and textile properties or plant species can be assessed without human intervention. Many other exam­ples abound in various industrial fields.

NIR cameras have introduced a new di­mension to this scenario: the spatial one. Today it is possible to measure NIR or Ra­man spectra in each pixel of a material surface, so that a data table is collected with spectro­spatial structure. These data are called hyperspectral, and allow one, among other things, to monitor the spa­tial distribution of chemical species on a surface. Applications include the study of the homogeneity of pharmaceuticals in solid tablets or pellets, the distribution of chlorophyll and other plant components in crop fields, etc., all made in a remote, non­invasive and automatic manner.

Where Is It Used?

The approach is not restricted to the digi­tal processing of spectra. Other instru­mental signals are slowly entering the analytical scene, whether optical (fluores­cence, mid/far infrared, laser induced breakdown, nuclear magnetic resonance) or electrical (sensor arrays, impedance spectroscopy, voltammetry). Other scien­tific fields make use of similar approach­es, e.g. bird species can be automatically classified from their sound, musical emo­tions and moods can be predicted from audio records, business cycles can be forecasted in econometric studies. It is a world open to scientific exploration as never before.

Exciting as it may seem, however, mul­tivariate spectral calibration is not the analytical panacea. Infrared spectral sensitivity is rather low, so that detecting traces of analytes is not an easy task, and some analyte signals are difficult to measure (e.g., metallic elements), mean­ing that chromatography and atomic spectroscopy are still needed. Moreover, to be able to cope with the presence of interferents, the mathematical multivari­ate models need to be properly trained. This means that a large and diverse ref­erence sample set is required for model building, usually involving hundreds or thousands of samples, for which nominal analyte values or target properties should be previously measured by classi­cal techniques. Furthermore, the model performance needs to be monitored over time, because there is nothing to prevent future samples containing new interfer­ents, not present in the calibration set. Should this occur, re­calibrating the

Multivariate and Multiway CalibrationsWhat Analytical Chemists Would Ask from Aladin’s Lamp

▪ Alejandro C. Olivieri1

The holy grail of chemical analysis is the monitoring of sample properties or concentra-tions of selected substances, remotely, non-invasively, automatically, and avoiding the use of solvents or specific reagents. This can be achieved by measuring certain optical signals, e.g., near infrared or Raman spectra, provided they are processed by multivari-ate calibration models, appropriately trained with a large and diverse basis set of refer-ence samples. More complex signals obtained by hyphenated chromatography or ma-trix fluorescence spectroscopy provide an even more revolutionary bonus: quantitating analytes in complex interfering samples by calibrating with a handful of pure standards.

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20 Spectroscopy

Special Topic

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G.I.T. Laboratory Journal 1/2020

model is required, adding the new inter­ferents to the data base. These undesir­able features can be overcome, however, by going one step further in the number of mathematical dimensions of the mea­sured data.

The Mathematical View

From a mathematical perspective, spectra can be viewed as vectors (lists of numbers one below each other). However, more complex data can be measured and pro­cessed, and this is when we move to the multiway calibration field. For example, a liquid chromatograph hyphenated to a di­ode array detector is able to measure a data table for a given sample, i.e., a data matrix. Its columns are UV­visible spec­tra, each of them collected at a different elution time. In an analogous fashion, a gas chromatograph with mass spectral detection can measure a data table whose columns are mass spectra. One could also employ a fluorescence spectrophotometer to measure an excitation­emission fluo­rescence matrix for each sample. Its col­umns are emission spectra, each of them collected at a different excitation wave­length. Scanning emission spectra at vari­ous excitation wavelengths is today possi­ble in a matter of seconds using modern fast­scanning spectrofluorimeters. Less popular in industrial laboratories, fluores­cence matrix spectroscopy has played a

major role in developing multiway cali­bration, and is highly appreciated in sci­entific research. In principle, the com­plexity and number of data modes (the independent directions of a data array) can be increased, and some developments have been described by processing three­ and even four­dimensional instrumental data per sample. However, this higher multiway protocols are still in their infan­cy regarding their mathematical under­standing and potential analytical advan­tages.

Fig. 1: Hierarchy of data structures and nomen-clature. For a single sample, univariate data are scalars (zeroth-order), multivariate data are vec-tors (first-order) and beyond, and multiway data are matrices (second-order), three-dimensional arrays (third-order) and additional, not shown, higher-dimensional data. For a sample set, the corresponding data arrays are named as one-, two-, three- and four-way arrays respectively.

Fig. 2: Univariate chromatogram for a mixture of polycyclic aromatic hydrocarbons, carried out accor-ding to the official protocol, and involving fluorescence detection at a single wavelength and solvent gradient. FLT, fluoranthene, PYR, pyrene, CHR, chrysene, BaA, benzo[a]anthracene, BbF, benzo[b]fluo-ranthene, BeP, benzo[e]pyrene, BjF, benzo[j]fluoranthene, BkF, benzo[k]fluoranthene, BaP, benzo[a]py-rene, DBA, dibenz[a,h]anthracene, BgP, benzo[g,h,i]perylene, IcP, indeno[1,2,3-cd]pyrene. The blue trace corresponds to the analytes; BeP and BjF are interferents.

THIS IS NOT A CHILLER AD.

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G.I.T. Laboratory Journal 1/2020

Multiway calibration and its outstand­ing properties are even more fabulous than those of multivariate spectral cali­bration. Just to give a common example, by measuring chromatographic­spectral matrices or data tables, you may be able to quantitate analytes in complex sam­ples, without the need of a large training set. Simply prepare a few pure analyte standards, measure their data matrices, join these data with those for the un­known sample, and let a multiway cali­bration model to mathematically separate the analyte contribution from those of the interferences. In other words, you do not need to worry about sample pre­treat­ment or clean­up, baseline resolution of every analyte peak or background correc­tions. Chromatographic protocols become simpler, isocratic, cheaper, faster and, perhaps more importantly, greener. The approach has been called chroMATHog­raphy, a nice game on words proposed by a famous chemometrician. A more techni­cal name for this property is second­order advantage.

A Successful Application

A case worth mentioning here is the de­termination of polycyclic aromatic hy­drocarbons (PAH) in aqueous samples. PAHs are environmentally concerned substances, many of them suspected to be carcinogenic to humans. The official liquid chromatographic protocol involves fluorescence detection and a mobile phase with solvent gradient, taking ca. 40 min to achieve baseline resolution. However, it is not free from potential in­terferents, and some non­regulated PAHs may coelute with the analytes. If liquid chromatographic matrices are measured with spectral (instead of single­wave­length) fluorescence detection, the job can be done under isocratic conditions and in less than 5 min. In our lab, ten highly co­eluting PAHs have been re­solved and quantitated at sub­ppb levels in aqueous samples using this methodol­ogy, even in the presence of uncalibrated interferents in unknown specimens. The resolution of the ten individual analytes, and their digital separation from the in­terferents, was possible by applying a powerful data processing algorithm known as multivariate curve resolution­alternating least­squares (MCR­ALS), which is based on the so­called bilinear model for matrix chromatographic data. Without going into further details, bilin­

ear means that the matrix data can be conceived as the product of two separate matrices, one of them containing pure component chromatograms and another one the associated spectra. After the de­composition phase, the ‘virtual’ pure chromatograms can be employed in a classical manner to produce a calibra­tion line, where the test sample signal is interpolated to yield the concentration of a specific analyte.

Fluorescence spectroscopy itself is also able to yield data matrices, by collecting emission spectra at a number of excita­tion wavelengths. The technique has al­lowed researchers to develop protocols for many different analytes in really com­plex natural or industrial samples. In fact, the first report showing that the second­order advantage was possible, published in 1975, described the determination of a polycyclic aromatic hydrocarbon in the presence of other fluorescent congeners, calibrating only with pure analyte solu­tions. No one suspected at that time that

multiway calibration would be so revolu­tionary to analytical chemistry. A nice se­quel of this work was recently developed in our lab: the determination of four PAHs on a nylon membrane attached to a rotat­ing disk, which was left in contact with aqueous test solutions for a few minutes. Fluorescence matrices were then read di­rectly on the membrane. Thanks to the pre­concentration properties of nylon, the protocol allowed the quantitation of indi­vidual analytes with detection limits in the range from 20 to 100 ng L−1, i.e., 20 to 100 parts­per­trillions!

Why Is There No Widespread Adoption?

Even with all these almost unbelievably useful properties, there are no crowds of analytical chemists knocking on the door of multiway calibration. This implies that considerable work needs to be done on the communication side between chemo­metricians and end analytical users. Re­grettably, there are many valuable re­sources for the analytical community buried in highly specific journals devoted to pure chemometrics. The communica­tion issue has been addressed in a recent meeting (Topics in Chemometrics, TIC, Szeged, Hungary, May 2019), where one researcher suggested that the chemome­tricians should be out there, offering the digital products to chemists, rather than trying to solve problems that do not exist, or waiting until chemists come to them. Chemometricians may have the answer to the Chemist’s problem – so talk to each other!

Affiliation1Departamento de Química Analítica, (IQUIR­CONICET), Rosario, Argentina

ContactProfessor Alejandro C. OlivieriUniversidad Nacional de RosarioInstituto de Química de RosarioRosario, [email protected]

Related Articles https://bit.ly/WAS-Calibration

Literatur: https://bit.ly/WAS-Olivieri

Fig. 3: Top: three-dimensional landscape of an elu-tion time-fluorescence emission wavelength data matrix, recorded for a mixture of similar composi-tion to the sample of Figure 2, but under isocratic conditions and in a much shorter time. Bottom: pure analyte chromatograms obtained by multi-way calibration of elution time-fluorescence emis-sion wavelength matrices. Individual analyte de-termination proceeded even when interferents are present in test samples. Analyte acronyms as in Figure 2. Reprinted with permission from [1]. Copyright 2009 American Chemical Society.

22 Spectroscopy

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G.I.T. Laboratory Journal 1/2020

The Digital LaboratoryA Short Introduction

J Martin Graf-Utzmann

We all have heard the phrase: digitisation is unstoppable. In a coming series of articles we would like to highlight what we believe to be the most important aspects of digitiza-tion and the associated automation in the laboratory.

Many people think of automation on an industrial scale. That means a deserted production hall in which robots on the as­sembly line assemble metal parts into cars with uncanny speed and precision. In this series of articles, automation starts on a small scale. For me, a script that per­forms a repetitive task „at the push of a button“ is already automation. The ma­chine takes over a part of the work that is repetitive and ultimately boring for me and therefore error­prone. The computer doesn‘t care how often values have to be written down, added, averaged, or pro­cessed in whatever way. In microbiology, for example, counting colonies in a petri dish can be done by an app on a smart­phone, in other words, automated. There are solutions already available on the market that make pipetting into microti­tre plates visually trackable and with that less error­prone. The system also takes care of the documentation. These are the first steps in transferring a task that does not involve any added value to a machine. Employees can use the time for more in­teresting and value adding tasks.

One Size Never Fits All

Of course there is no general solution to satisfy all users in all laboratories. How­ever, the market offers solutions that can be implemented in your laboratory right now. For special cases tailor­made solu­tions can be created in cooperation with the suppliers. The first step is to find out where time­consuming processes can be sensibly automated and digitalised. The interaction of automation and digitiza­tion is what makes this setup so power­ful.

But keep in mind that digitization re­quires thorough preparation. The pro­cesses to be automated must be well un­derstood. After all, who benefits if a bad analog process is replaced by a bad digi­tal process? It is also essential to involve the people who work in the laboratory in the planning, as they will have to use the new processes. The acceptance of the em­ployees is decisive for the success of a

digitisation project. Processes that are au­tomated but cause more work elsewhere have little chance to be accepted. On the other hand, if the work becomes more in­teresting by leaving out the „boring“ ac­tivities, everyone wins.

So - What Can you Expect?

In this issue of G.I.T. Laboratory Journal you will find an article by Dr. Thorsten Teutenberg from IUTA e.V. in Duisburg. Dr. Teutenberg is setting up an automated laboratory with funds from the state of North Rhine­Westphalia. The name of the project, on which we have reported on several occasions in our magazine, is Fu­tureLab NRW. The article offers an insight into the possibilities of collaborative ro­botics.

Prof. Dr. habil Kerstin Thurow from the Center for Life Science Automation (celis­ca) in Rostock takes a different approach in her article and presents her point of view on the topic of collaboration with ro­bots in the laboratory.

The editorial team and I look forward to presenting further insights and points of view in the course of the year within the series of articles. If you would like us to cover a certain aspect of digitization, please feel free to send us an e­mail with the subject line „Digitalisation“ to git­la­[email protected].

ContactDr. Martin Graf-UtzmannEditor-in-Chief, G.I.T. Laboratory JournalWiley-VCHWeinheim, [email protected]

Related Articles http://bit.ly/WAS-Robotics

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G.I.T. Laboratory Journal 1/2020

The First Steps

In order to come close to the ideal of a completely digital planning, control and documentation of an analytical process,

laboratory equipment as well as the per­sons involved are to be registered in a central management system. The greatest technical challenges here are the defini­tion and establishment of suitable inter­

faces between a conventional laboratory information and management system (LIMS), an electronic laboratory journal (ELN) and a laboratory execution system (LES). Furthermore, the connection and interfacing of all devices and the integra­tion of existing data and upcoming data is of utmost importance. Many of the de­vices currently in use do not have suitable hardware or software interfaces. There­fore, they can only be regarded as stand­alone solutions. In order to guarantee an automated data transfer, integration solu­tions are necessary, which additionally enable the acquisition and exchange of metadata (e.g. information about the type of measurement and the person operating

The Bot on the BenchCooperative Robotics and the Routine Lab

▪ T. Teutenberg1, K. Kochale1, M. Jochums1, M. Dronov1, L. Gehrmann1, N. Abdulin1, and J. Tuerk1

In spring 2016, the North Rhine-Westphalian state government launched an initiative to promote research and innovation potential. The application and implementation orien-tation in science and industry is to be strengthened in a targeted manner through sus-tainable and intelligent further development of existing research structures. This is meant to align the competencies of all players with the social challenges of the future. One of the projects funded is “FutureLab NRW”, in the context of which the Institut für Energie- und Umwelttechnik e. V. (Institute for Energy and Environmental Technology, IUTA) will implement the infrastructure for a digitized model laboratory for the miniatur-ized instrumental and effect-based analysis of the future.

Figure 1: Experimental set-up for the implementation of central automation steps.

© IU

TA

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the device). In addition, the central man­agement system should be designed with sufficient flexibility to be able to take over the administration of new types of labora­tory components (e.g. intelligent, func­tionalized laboratory furniture) and dif­ferent types of sensor systems (e.g. for monitoring the room temperature).

Besides the question of higher­level standards, collaborative robotics will play an important role. Figure 1 shows a first experimental setup for the imple­mentation of central automation solu­tions. The robot shown in the figure 1 is initially intended to perform simple “pick and place” tasks that are normally car­ried out by qualified laboratory person­nel. For humans, these are generally simple activities, such as loading an au­tosampler for HPLC with the correspond­ing sample trays. The transfer of this process to a robot is highly complex, very susceptible to interference and may require in­depth programming knowl­edge. In order to meet the problem of skilled worker shortage, especially in the area of highly specialized IT profession­als, an intuitive software is used for the first time. This allows the configuration and parameterization of basic functions of the robot by “drag & drop” to complex process chains. In this way it is not nec­essary for the user to have profound pro­gramming knowledge. Instead, the soft­ware translates the individual motion sequences as well as the force control of the robot into native program code, so that subsequent editing of the source code by a programmer is always possi­ble. This experimental setup is intended to provide information as to whether and to what extent a domain expert (labora­tory technician or technical employee) can automate work processes in the lab­oratory, without having to rely on corre­sponding IT specialists.

What’s Next?

After successful establishment of first workflows and the integration of all de­vices, the transfer to a mobile platform is planned. Against this background, IUTA has been a member of the Self­driving Lab Robots Interest Group since 2019. Members of this group are mainly the large leading pharmaceutical companies. The objective is, among other things, to create a software platform that enables the integration of laboratory devices un­der a uniform standard. In this context, the IUTA FutureLab  NRW can establish itself as an important link between indus­try and science by working on scientific­technical issues that cannot yet be imple­mented in real operation even in the

largest companies due to, for example, current technical risk.

Acknowledgement

The funding from the State of NRW will be provided using resources from the Euro­pean Regional Development Fund (ERDF) 2014 ­ 2020 “Investments in growth and employment”.

Affiliation1IUTA, Duisburg, Germany

ContactDr. Thorsten TeutenbergIUTA e.V. Duisburg, [email protected]

Related Articles: http://bit.ly/WAS-Robotics

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Laboratory Automation

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G.I.T. Laboratory Journal 1/2020

The term “cobot” is an abbreviation of “collaborative robot” and basically refers to industrial robots that are not separated from humans in the production process, but rather work together with them. In the 1997 patent, J.E. Colgate and M.A. Peshkin defined cobots as follows [1]:

“an apparatus and method for direct physical interaction between a person

and a general purpose manipulator con­trolled by a computer”

The establishment of the cobots was a significant further development of the classic industrial robots, which usually work completely separate from people. By integrating numerous sensors that im­prove safety, e.g. switching off when touching an obstacle, collaborative robots

can work in close proximity to humans or work directly with humans. Expensive protective devices such as housings or light barriers / curtains can therefore be omitted. Current versions of the stan­dards ISO 1028 part 1/2 as well as ISO / TS 15066 define the safety requirements also for collaborative robots [2], [3], [4].

Cobots combine the classic advantages of robots such as power, high precision and reproducibility and endurance with human characteristics such as experi­ence, creativity or the general overview and open up completely new possibilities and applications.

Collaboration?

Even if the word “cobot” is derived from the term collaboration, real collaboration between humans and robots is only the closest form of collaboration. Coexistence, in which humans and robots work in close proximity without a shelter, is most common, but human and robot do not share the workspace. If humans and ro­bots share a workspace, we speak of co­operation. This can be for example trans­fer stations where people transfer a part, workpiece or sample so that the robot can pick them up. Humans and robots work in a common space, but at different times. The closest mode of operation is collabo­ration, in which humans and robots work on a part / workpiece at the same time (although both perform different tasks).

Numerous cobots have entered the market in recent years, initially under the more general name of ‘lightweight ro­bots’. Companies like Kuka, Universal Ro­bots, ABB, Rethink, Kawasaki, Yaskawa, Franka Emika or Denso offer numerous systems today.

How important are cobots in laboratory automation? Due to their lightweight con­struction, they have numerous advantag­es. Laboratory applications usually do not have the load capacity requirements as they exist in classical industrial areas. Classical industrial robots are often over­engineered in the laboratory. This also has a significant impact on the price of the ro­botic systems. The modern cobots are powerful systems that are also character­ized by moderate prices. The possible omission of safety enclosures and light barriers is also an advantage. Cobot­based automation systems therefore take up less

Cobots in the Analytical Laboratory Useful Tool or Gadget?

▪ Kerstin Thurow

We are currently experiencing a real cobot hype. Search engines like google now deliver more than 861,000 results. Everyone is talking about cobots today and they are also gaining increasing interest in laboratory automation. But what are cobots? Are they re-ally useful tools in laboratory automation or just a nice toy?

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26 Laboratory Automation

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G.I.T. Laboratory Journal 1/2020

space and also allow a more flexible use of integrated sub­components such as opti­cal readers, centrifuges or analytical mea­surement systems (GC, LC, MS) if these are not used in the robot process.

But can cobots really be used in a col­laborative way in the laboratory? Hardly likely. The number of processes in which robots and humans work together on one specific task is likely to be very low. It is hardly conceivable that classical laborato­ry work such as pipetting, weighing, shak­ing, extracting or recording measured val­ues will be processed together by humans and robots. Cooperation and coexistence remain as possible forms of cooperation. In the latter, the cobots are used in auto­mated systems in which classical industri­al robots were used in previous concepts. The overall concept of automation is not changing. Due to the lower costs, automa­tion of sample preparation and measure­ment technology are now possible in new areas, which did not use automation previ­ously due to cost reasons. This makes au­tomation of laboratory processes increas­ingly interesting and affordable for small and medium­sized companies as well as for research institutions. Here, flexible ful­ly automated systems (automation lines) will be the focus of interest in order to be able to process larger numbers of samples at better prices. However, it should be not­ed that not all robots are also equipped with suitable control software. Software components can be purchased from exter­nal companies or must be developed in­house. Depending on the scope of the task, considerable costs may arise.

More Cobots - More Problems?

Due to the low cost of the cobots, it is in principle also conceivable to equip differ­ent laboratory devices with robots. These could, in particular in the case of measur­ing systems, liquid handling systems, shakers, heaters and other laboratory de­vices, function as a transfer unit and feed the samples placed by humans to the re­spective devices or remove them again af­ter the corresponding process times have expired. This corresponds to the coopera­tive mode and would make the laboratory work considerably easier. The existing laboratory environment and structure could be largely preserved, extensive re­structuring is not necessary. There are several things to consider with this ap­proach. If many laboratory devices are to be equipped with robots, the number of

cobots required results in high investment and maintenance costs. Simple transfer processes usually do not require a large number of degrees of freedom, i.e. cobots would also be overengineered in this case and could be replaced by simpler systems with fewer degrees of freedom.

The biggest problem, however, is the control of the systems. Superordinate control systems are required, especially if samples have to be processed at several stations and several robots and laborato­ry devices have to be managed and con­trolled. Depending on the desired scope of options and flexibility, these workflow management systems can quickly become very extensive and therefore also expen­sive.

Summary

So is the current cobot hype really justi­fied? Cobots are a sensible and logical further development of classical industri­al robots. Their possible use and the type of use (coexistence, cooperation, collabo­ration) are very dependent on the respec­tive application. In the field of laboratory automation, the first two possibilities will surely prevail in the coming years due to the tasks and requirements. In the coop­erating sector, a special form of cobots makes sense for different laboratory sta­tions and transport between the stations: mobile robots. These can either only real­ize transport tasks between different sta­tions or they can also take over the supply of samples to individual laboratory de­vices. This can limit the total number of robots required. However, the require­ments for the workflow management sys­tems remain and are additionally in­creased by a mobility component. Mobile robots are currently being used in the au­tomation area. Due to the high costs (comparable to classic industrial robots), they are not yet a real alternative.

ContactProf. Dr.-Ing. habil. Kerstin Thurowcelisca, University RostockRostock, [email protected]

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Laboratory Automation 27

Articles

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G.I.T. Laboratory Journal 1/2020

The idea of using molecules as electronic components is in line with a general ten­dency followed by modern electronics, the miniaturization of individual components. The great promise of organic molecules over existing semiconductor electronic el­ements are: 1) their size – most of the simple molecules are significantly smaller than existing individual transistors, 2) their uniformity – molecules of the same compound are identical to the last atom, and 3) the flexibility in design – chemistry provides various well­established routes to tune the properties of molecules. These prospects paved the road for further in­vestigations in the field.

Creating Electrodes on the Atomic Scale

A very successful technique to character­ize electrical transport at the nanoscale

are mechanically controlled break junc­tions (MCBJ). Historically, they were de­veloped to study the properties of weak links in superconducting materials using freshly broken metallic surfaces. The technology was then adapted for the study of atomically­sharp metallic elec­trodes where the quantization of the elec­trical conductance can be observed, and further on for the measurements of mol­ecules [2, 3]. Nowadays, the MCBJ ap­proach is one of the most common exper­imental techniques in the field of molecular electronics. The technique re­lies on the breaking of a metallic wire by pulling it in a controlled way. This is achieved by bending a flexible substrate on which a metallic constriction has been fabricated. Using modern nanofabrica­tion techniques, metallic nanobridges with an attenuation factor (the ratio be­tween the wire elongation and the sub­

strate bending) below 10­4 can be achieved. In other words, bending the substrate by 1 micrometer leads to a wire elongation of less than 0.1 nanometer, re­sulting in remarkable mechanical stabili­ty and tunability of the system. The breaking of the metallic wire is moni­tored by applying a potential difference between its two ends and measuring the resulting current in the circuit (Fig.1). The magnitude of the current decreases as the metal wire is stretched until it ulti­mately consists of a single atom. Upon bending the substrate even further, the nanobridge is broken, accompanied by a drastic change in current as the resulting tunneling gap between the two atomically sharp electrodes slowly increases. To per­form the electrical characterization of molecules, the latter are functionalized with ‘anchor groups’ – chemical groups that provide covalent or van der Waals binding to the electrodes and allow mole­cules to bridge the gap between them. Typical anchor groups are, for instance, thiols (­SH), amines (­NH2), cyanides (­CN) and pyridils (­C5H5N), with gold be­ing the predominantly used electrode material. The properties of molecular junctions formed in this way are defined

Molecular ElectronicsBuilding Organometallic Chains Molecule by Molecule

▪ Anton Vladyka1, Jan Overbeck1, Mickael Perrin1, Michel Calame1

The concept of single-molecule electronics – the use of individual organic molecules as active elements in electrical circuits – strongly roots in the inspirational theoretical con-tribution by Aviram and Ratner where a molecular diode was proposed [1]. We describe here recent experimental progress showing how an organometallic chain can be as-sembled molecule by molecule between metallic electrodes.

Fig.1: Illustration of a break junction for measurements in liquid environment. The typical length of free-standing gold bridge (u) is 300-500 nm, and the width of its constriction is 60-100 nm. Figure adapted from [4].

28 Chemical Syntheses

Articles

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G.I.T. Laboratory Journal 1/2020

by: 1) their chemical backbone, 2) the an­chor groups, 3) the nature of the elec­trodes, and 4) the environment (e.g. a sol­vent).

Molecular Signatures

During the measurement process the junction is repeatedly broken (opened) and then reformed (closed) in the pres­ence of molecules in solution. For every cycle, the change in conductance with electrode separation (conductance trace) is recorded. One or few of the molecules from the surrounding medium can bridge the gap between the electrodes, and this event is reflected in the conductance trace. In this way, the binding and un­binding of a single molecule in a nanome­ter­sized gap can be detected in real time. To capture the large amount of possible conformations of the molecule(s) between the electrodes, this process is repeated hundreds of times, and then statistically analyzed.

The data are usually presented as ei­ther conductance histograms or conduc­tance­displacement histograms (Fig.2). In a conductance histogram, the probabili­ty­distribution of conductance values during the entire breaking process is pre­sented. If the target molecule forms sta­ble molecular junctions, a peak appears in the conductance histogram. For the conductance­displacement histogram, a two­dimensional distribution of conduc­tance values as a function of electrode displacement (roughly the gap size) is presented. In this case, the signature of molecular junction formation is a con­

ductance plateau (i.e. a relatively flat area of high counts).

Knitting with Molecules

Because future applications based on mo­lecular junctions require their stable inte­gration into electronic circuits, studying the interaction of molecules with elec­trodes is of particular interest. In a recent study, we used the MCBJ approach to characterize BdNC molecules [4]. These are benzene molecules that have highly polar isocyano (­NC) chemical groups as anchors. The interaction of isocyanides with gold is known to be particularly strong, with calculations confirming their binding energy to surpass even that of co­valent sulfur­gold bonds. Moreover, the isocyano­gold bond is highly directional allowing for dense molecular surface cov­erage which makes these molecules an ideal test system for reliable junction for­mation.

The measurements were performed on a 100 micromolar solution of BdNC molecules in a mixture of THF and me­sitylene. Multiple conductance plateaus were observed in the conductance histo­grams of opening­traces with a very high yield of plateau formation (Fig.2). This can be explained by the formation of multiple stable molecular configura­tions during the measurements process. In notable contrast to break junction measurements on most other molecules, a similar behavior was also observed for the closing conductance traces, i.e. while the electrodes are approaching. This is a result of the polarity of the isocyano­

Fig.2: Conductance-displacement histogram of BdNC-molecules in solution. Three areas of high counts as well as three peaks on the conductance histogram (right) are the signatures of three con-secutive plateaus in conductance traces which are referred to stepwise chain formation. The inset shows the si-tuation when the third plateau is not formed. Figure reproduced from [4].

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gold bond resulting in molecules sticking out from the surface, ready to form a contact.

To attribute the conductance signatures to specific molecular configurations we performed theoretical calculations using Density Functional Theory based Molecu­lar Dynamics (DFT­MD) simulations at room temperature.

These allow us to visualize the interac­tion of molecules while pulling apart the electrodes at a temporal resolution of 1 fs. Evaluating several sets of these com­putational experiments, we are able to link molecule­electrode configurations to observed conductance features. The first conductance plateau with higher conduc­tance was attributed to the single­molec­ular junction and its conductance value is similar to that observed in other mole­cules of comparable structure. The sec­ond, lower conductance plateau, corre­sponds to the configuration of organometallic chains which include two molecules plus one or more additional gold atoms. These chains form during the opening process because the strong interaction between the isocyano an­chor­group and gold allows the BdNC molecule to pull a gold atom from the electrode. The resulting organometallic compound can then interact with anoth­er molecule which is present in close proximity to the junction as a conse­quence of the dense surface packing de­scribed above. Detailed analysis reveals that in 29 % of all breaking traces a third plateau is observed, which is attributed to the formation of trimer chains at even larger electrode displacement and with correspondingly lower conductance.

Even longer chains are expected to form, however, with conductance below the de­tection limit.

Tuning Knobs

As the on­surface concentration of mole­cules in the vicinity of the initial mono­molecular junction is of prime importance for the chaining process, we employ two complementary approaches to exercise control over organometallic chain forma­tion. First, the same measurements were performed in solutions with smaller con­centrations of BdNC molecules to reduce the equilibrium density of molecules on the electrode surface. In the second ap­proach, the central benzene ring of the molecule was extended by two bulky side groups, namely methyl and tert­butyl in­stead of the usual hydrogen termination. These side groups are expected to de­crease the effective electrode surface cov­erage through steric hindrance, and hence suppress the formation of dimers and trimers. Indeed, experimentally both routes were tested and yielded a suppres­sion of the oligomerization process. In­stead, a single high­conductance plateau with modified shape (width and slope) was observed and attributed to a slightly modified configuration statistics of the mono­molecular junction.

Microscopic Understanding

The formation of molecular dimers and trimers through incorporation of gold at­oms can be considered as a controlled step­by­step synthesis of such a conduc­tive metal­organic 1D­oligomer. Here, the

exceptional stability of the MCBJ tech­nique allows for the real­time observation of the process of chain formation, mole­cule after molecule, as revealed by their conductance properties. In stark contrast to other synthetic schemes, the process does not rely on the stepwise addition of different constituents of the organometal­lic compound, but occurs via in­situ ex­traction of atoms from the electrode by the isocyano­molecules. These findings pave the way for the controlled formation of one­dimensional, single coordination chains, which may be used as promising building blocks for organometallic frame­works.

Affiliation1Transport at Nanoscale Interfaces, Empa, Dübendorf, Switzerland

ContactMichel Calame Head of LaboratoryTransport at Nanoscale InterfacesEmpaDübendorf, [email protected]

Related Articles http://bit.ly/WAS-MatSci

More Information on http://bit.ly/GLJ-ME

References: http://bit.ly/GLJ-Calame

Fig.3: DFT-MD calculations demonstrating the chaining of two BdNC molecules while the gold elec-trodes are withdrawing. Figure reproduced from [4].

30 Chemical Syntheses

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The project is funded as part of the Euro­pean Union’s Horizon 2020 work program and is coordinated by Professor Albert Heck, project coordinator and professor of biomolecular mass spectrometry and pro­teomics at Utrecht University.

Why Now?

The idea to form a platform where all sci­entists in the field of proteomics can fur­

ther develop their skills, share their ex­pertise, and provide access to top of the range mass spectrometry equipment is not a new one. The high cost of mass spectrometry equipment has long been a precluding factor for the widespread use of proteomics for many institutions and clinical laboratories engaged in life sci­ence research. Most proteomics technolo­gies use complex instrumentation, critical computing power, and expensive consum­

ables. Taken together with the need to ac­quire, train and retrain highly qualified research staff, long­term sustainability of mass spectrometry equipment can be a challenge. As a result, the major contribu­tors to progressive advancements in the field of proteomics are largely made by a small group of pioneers in the field; many of whom are located in Europe.

It was realized from a previous Euro­pean proteomics project, Prime­XS, which facilitated access for life science research­ers to high­end mass spec infrastructure and expertise from pioneers in the pro­teomics field, that it had been successful in achieving a more coordinated effort within the proteomics community throughout Europe. The initiative (which finished in 2015) resulted in the publica­tion of more than 300 articles and paved

European Proteomics Infrastructure Consortium – Providing AccessA European Proteomics Initiative

▪ Martina O’Flaherty

The European Proteomics Infrastructure Consortium - Providing Access (EPIC-XS) part-nership is a project funded by the European Union to provide proteomics expertise and mass spectrometry technology to all researchers within the life science arena. It brings together eighteen proteomics institutes, spread across fourteen European countries, with the objective to provide over 2,400 days of access to high-end proteomics technol-ogies. This initiative will also provide access to various workshops and training courses.

European Proteomics Infrastructure Consortium – Providing Access (EPIC-XS) research community, representing countries spread across fourteen European member states.

32 Mass Spectrometry

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the way for a new initiative – the European Proteomics Infra­structure Consortium ­ Providing Access (EPIC­XS).The EPIC­XS community has now expanded its consortium to in­clude seven new European partners and will again provide ex­pertise and access to proteomics technology to European re­searchers.

Why Proteomics?

Protein analysis is essential for understanding the complexity of the communication process that takes place within every living organism. This is important because proteins essentially repre­sent the actual function of a cell.  In order to contribute to ad­vancements within the life sciences it’s important that we under­stand how proteins work. If we can figure out how proteins express themselves, how they function, and interact with other proteins, metabolites and nucleic acids, then we can gain valu­able insight into potential disease biomarkers that can ultimately be used for detection, treatment and in some cases, disease pre­vention. The study of the interactions between all proteins, the proteome, is called proteomics.

Unravelling the secrets of the proteome is a challenging task, some may say even daunting, considering that every individual is unique and has an entire set of proteins which are constantly changing and reorganizing themselves and because protein ex­pression spans many more orders of dynamic range than DNA and RNA, they are more difficult to work with. As proteins can­not be amplified like DNA and RNA, less abundant species are more difficult to detect, hence improving sensitivity and the dy­namic range of proteomics analysis is essential and quite chal­lenging. To complicate matters even more, is the fact that there may be more than several million distinct protein molecules in a cell, each having their own potential properties and functions and all of which undergo a certain degree of post­translational modifications. Thus, analysis of the entire proteome presents a surmountable challenge. The goal of getting rid of high abun­dance components in biological fluids, tissues, organisms and cells in order to identify those proteins which contribute to ill­nesses and disorders and identifying those which could be used as potential biomarkers, has been the focus of many proteomics laboratories around the world. In achieving this goal, mass spec­trometry has become one of the most powerful tools in the field of proteomics. This technology measures a fundamental charac­teristic of a molecule, its molecular weight. The measurement of the mass­to­charge ratio of ions helps identify and quantify mol­ecules in simple and complex mixtures. Valuable information with regard to the analysis of low molecular compounds and macromolecules present in protein samples can be gained by use of this technology. Modern instruments feature very power­ful analytical capabilities – sensitivity, selectivity, resolution, throughput, mass range, mass accuracy – so much so that these days pretty much any protein in a cell is identifiable. In fact, over the last ten years due to advancements in mass spectrometry technology and its cost effectiveness, that proteomics has evolved from a very specialized discipline to becoming a much more standard fixture in the laboratory [1,2].

Proteomics has also played an important role in cancer re­search [3]. It allows for monitoring drug responses of tumors, understanding mechanisms that lead to cancer pathogenesis, de­signing novel therapeutics, and even makes it possible to identify cancer cells in biopsies [4]. Some of the most exciting break­throughs in proteomics involve the discovery of new biomarkers. One of the pioneers in the proteomics field, Ruedi Abersold of the ETH Institute in Switzerland, has employed proteomic profiling techniques to do exactly that [5].

Making translational proteome profiling a more mainstream technology in a clinical environment is also a project which will be addressed within the EPIC­XS initiative. Matthias Mann from the University of Copenhagen in Denmark leads this project. The team of researchers aim to address some of the most recent bot­tlenecks in clinical proteomics, by developing robust, reproduc­ible high throughput proteomics workflows to analyze large sample cohorts from patient samples. They will investigate sensi­tivity issues, tackling the dynamic range in the plasma proteome and address the use of PTM’s and epigenetics as biomarkers for disease states. These technologies will be made directly available to the scientific community through their implementation in the European Proteomics Infrastructure Consortium access sites.

There have also been significant developments in many other types of medical conditions such as Alzheimer’s disease, schizo­phrenia and depression that have been enabled by proteomics. Amongst others, John Yates of the Yates Laboratory at the Scripps Research Institute, USA, develop and apply proteomics to study these conditions.

While proteomics has helped researchers develop new drugs to fight diseases, this field of research is not limited to the medi­cal arena. Valuable information in relation to developing food products that are safe and contribute to our good health and wellbeing can also be attributed to proteomics. Interestingly, proteomics studies reveal that nutritional factors can play both a beneficial and a detrimental role in complex inflammation­relat­ed disorders such as allergies, asthma, obesity, type 2 diabetes, cardiovascular disease and rheumatoid arthritis [6, 7].

It is obvious that improving our understanding of how cells interact is crucial, and while researchers can now identify al­most any protein in a cell, the growing demands of identifying highly specific protein biomarkers in precision medicine means

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that many researchers are now turning their focus to the charac­terization of single cells.

The ability to monitor how the proteome differs from cell to cell presents quite a challenge. In an article in 2018 [8] Assistant Professor Jia Guo, of the School of Molecular Sciences, Arizona State University, Tempe, AZ, USA, explained that ‘single cell anal­ysis makes it possible to discover mechanisms not seen when studying a bulk population of cells.’

EPIC­XS researchers are also leading scientific investigations into the field of single cell proteomics. A consorted team within EPIC­XS will investigate the development of groundbreaking novel proteomics technologies improving sample throughput, post­translational modification analysis, sensitivity towards sin­gle cell analysis, reproducibility, dynamic range, multiplexing ca­pabilities and proteome coverage. The aim of this project is to go beyond state of the art, to bring proteomics into the future, tak­ing proteomics research one­step further.

Aside from the exciting future mass spectrometry holds within the field of proteomic and metabolomic analysis of single cells [9­11] this technology is by no means limited to the medical and diagnostic sectors. Focusing on the ever­increasing world popu­lation and global climate change, it is clear that the generation of new nutrient rich and sustainable food crops has become a ne­cessity. Many developments of novel designer crops can also be attributed to mass spectrometry technology [12, 13] as well as advancements, which have led to the discovery of proteins hid­den away not only within plant and animal life but also within other life forms such as tiny microalgae. The research team led by professor Albert Heck, professor of biomolecular mass spec­trometry and proteomics at Utrecht University in The Nether­lands, together with the proteomics team at Birmingham Univer­

sity have unraveled insights about the composition and structure of the light­harvesting system within this tiny microalgae life form, which can facilitate the development of new solar panels [14].

Why EPIC-XS, Why Now?

While this project will provide access to high­end proteomics technology which otherwise would not be accessible to many re­searchers, it’s also quite ambitious in its aim to tackle logistical hurdles presented by the plethora of datasets generated by pro­teomics methodologies, a subject close to the heart of many pro­teomics researchers.

Many laboratories find the mass spectrometry data difficult to handle and are working hard to overcome this issue. John Yates of the Yates Laboratory at the Scripps Research Institute, USA has also addressed this topic [15].

EPIC­XS researchers also have a team dedicated to computa­tional proteomics. The team will develop algorithms and soft­ware standards for data handling, providing support to all scien­tists across the consortium, for managing processes and data manipulation.

All the research activities within EPIC­XS are built on the strong innovative track­record of the consortium members in­volved and are focused on designing novel approaches for future developmental efforts not only in computational and translation­al approaches but also, in structural and spatial proteomics. This project team is led by Paula Picotti, at the Institute of Molecular Systems Biology, ETH, Zurich, Switzerland and will focus on characterization of the subcellular organization of the proteome, yielding important information regarding protein function. High­

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er order structure of proteins will also be investigated, a research area which is critical to our understanding of biological function because changes in higher order structure of proteins can have a detri­mental effect regarding the quality, stabil­ity, safety and efficacy of many medicines and can also lead to loss of biological function.

By providing the broader proteomics community access to experts in the field, and by enabling them to avail of high­end mass spectrometry technology, hardware and bioinformatics tools, EPIC­XS will help overcome many challenges facing re­searchers in this field of science. Trans­national access will also provide hands­on training for researchers, helping to develop best practice workflows, and aid the dissemination of proteomics data into publicly available databases thereby broadening the expertise of experienced scientists and those new to the field of proteomics.

The Future

It’s an exciting time to be involved in the field of proteomics and while the sensitiv­ity of MS has steadily increased over the years, further improvements are possible and necessary, especially if ever single cell analysis can be reached.  Limited mass spectrometry sensitivity, low dynamic ranges, undesirable long analysis time needed for clinical biomarker verification and validation, the lack of established and routine mass spectrometry based analyti­cal technologies, taken together with equipment costs are all precluding factors in the struggle to achieve and expand the sustainability of mass spectrometry within the proteomics arena. This is where EPIC­XS can help. This dedicated consortium of expert proteomic scientists is committed in their efforts to address these challenges and are positive about the outcome of se­curing a bright and sustainable future for all of life science research. That there’s a

high demand for such a European pro­teomics initiative can be seen by the num­ber of applications already received. Over ninety users have requested access to this technology and we are only at the start of this four­year project. Not only that, but this project has also realized a require­ment for initiative’s like this one to stretch further than its European borders, as ap­plications have also been requested from Canada and America. While funding may have run its course by the end of 2022, the sustainability of the proteomics communi­ty, through this initiative, will continue to strengthen and develop long into the fu­ture.

ContactDr. Martina O’FlahertyUniversity of UtrechtUtrecht, The [email protected]

More information on EPIC-XS http://bit.ly/epic-xs-eu

Interview with Ruedi Aebersold: http://bit.ly/Interview-Aebersold

Consortium members and references: http://bit.ly/GLJ-OFlaherty2

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Phenylurea pesticides, including diflubenzuron (DFB) and fenu­ron (FEN) are widely used in agriculture to improve productivity and, consequently, they can produce residues in crops, soils and surface waters. Due to the photochemical reactivity of these pes­ticides, photo­induced fluorescence (PIF) methods were devel­oped, based either on UV irradiation (classical PIF) or on direct laser irradiation (DL­PIF), for determining their residues [1,2]. Therefore, in order to validate the PIF methods, analytical appli­cations were performed in Senegal natural waters. Moreover, gas chromatography­mass spectrometry (GC­MS) was combined

to PIF to separate and identify the fluorescent DFB and FEN photoproducts by comparing them to standard compounds, in­cluding phenol and p­hydroxy­aniline [3].

Procedures

Fluorescence measurements were carried out at room tempera­ture on a Kontron SFM­25 spectrofluorimeter, interfaced with a microcomputer. Phototransformation of both pesticides into strongly fluorescent photoproducts was realized under UV or DL irradiation of the DFB and FEN working standard solutions (10­5 M). Liquid extraction of 3 mL of DFB and FEN irradiated solutions was carried out three times with 10 mL of ethyl acetate. Then, the organic phase was dried with anhydrous magnesium sulfate (MgSO4) in order to remove the traces of water, and afterwards was evaporated to dryness at 45 °C with a rotavapor. The dried residues were dissolved in 300 μL of ethyl acetate. A 200­μL sam­ple was supplemented to 1 mL, and a 1.5 μL volume sample was studied by GC­MS. A Nist library X calibur software was utilized to interpret the mass spectra (m/z values ranging from 50 to 650). All experimental conditions were optimized in our previous work [1].

Identification of the Fluorescent PhotoproductsAnalysis of Two Phenylureas by Photo-Induced Fluorescence (PIF) and GC-MS

▪ P. A. Diaw1,2,3,4, O. M. A. Mbaye2,3,4, D. D. Thiaré2, N. Oturan3, M. D. Gaye-Seye2,3, A. Coly2, B. Le Jeune2, P. Giamarchi4, M. A. Oturan3, J.-J. Aaron3

Quantitative analysis of diflubenzuron (DFB) and fenuron (FEN) pesticides was successfully performed using photo-induced fluo-rescence (PIF) methods. The analytical conditions were optimized for the determination of traces of these pesticides in Senegalese natural water samples. Mean recoveries were satisfactory, rang-ing between 80 and 120%. Also, gas chromatography-mass spectrometry (GC-MS) was combined with the PIF methods, in order to identify the formed fluorescent photoproducts.

G.I.T. Laboratory Journal 1/2020

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Analytical Performances and Applications

First, the analytical performances of both PIF methods were studied. For the classical PIF method, strongly fluorescent pho­toproducts were obtained at λex/λem = 331/405 nm for DFB in pH4 water­methanol (30:70, v/v) mixture and at 282/343 nm for FEN in pH4 aqueous solution, with low limit of detection (LOD) values of, respectively, 9 and 28 ng mL­1. In the case of the DL­PIF method, the excitation/emission fluorescence ma­trix also revealed very fluorescent photoproducts at λex/λem = 240/342 nm for DFB in pH4 water/methanol (30:70, v/v) mix­ture and at 240/308 nm for FEN in pH4 aqueous solution, with LOD values of 4.5 and 1.5 ng mL­1, respectively. A LOD value of 5 ng mL­1 in propanol­2­ol was also reported by Coly and Aaron [4] for the PIF determination of DFB in technical formu­lations. For analytical applications, a liquid­liquid extraction was applied using dichloro­methane as extracting solvent. Standard addition method and direct spiking procedure were applied to determine mean re­coveries. Standard addition slopes were found to be very close to those measured for the calibration curves. For both methods, the mean recoveries of DFB and FEN in Senegal nat­ural water samples (tap, river and sea water) ranged between about 80 and 120% with rela­tive standard deviation values below 10 %, according to the procedure and type of water sample [1,2].

Interference Studies of Added Foreign Species

Since several commonly used pesticides, namely fluometuron, monolinuron, linuron, carbaryl, pendimethalin and propanil, as well as various inorganic ions (Ca2+, (PO4)2

3­; K+, NO3­, Na+,

CO3­), were generally found in

the Senegal natural waters, their possible interference ef­fects on the determination of DFB and FEN was investigated. The DFB and FEN concentra­tions were respectively fixed at 0.1 mg  mL­1 and at 0.015 mg mL­1. The tolerance limit of the interfering foreign species was defined as the concentra­tion limit of these interfering species for which the percent­age of PIF signal variation did not exceed ± 5% in the determi­nation of DFB and FEN. Addi­tion of foreign species neither changed the shape of DFB and FEN PIF emission spectra, nor shifted the maximum emission wavelength. But, significant PIF intensity changes occurred with

increasing concentrations of foreign species. In the case of the DL­PIF method, the addition of increasing concentrations of DFB (up to 2 µg mL­1) to a FEN solution (1 µg mL­1) did not af­fect the FEN PIF spectra, because the DFB photoproducts were only formed in a water/methanol mixture, but not in pure wa­ter. In contrast, FEN produced relatively high interference ef­fects, since a FEN concentration as low as 0.04 μg mL­1 in­creased the DFB PIF signal above the tolerance limit. It might be explained by the formation of the same PIF FEN photoprod­uct at λem = 342 nm. To overcome the interference, the PIF sig­nal obtained in water/methanol mixture was corrected by the FEN fluorescence obtained from PIF measurements in pure water [2]. Therefore, a correction factor was applied to take into account the fact that the fluorescence quantum yield of the FEN photoproduct was higher in water/methanol mixture than

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in pure water. This correction minimized the interference ef­fects and improved the PIF selectivity.

Identification of the Fluorescence Photoproducts

Only one fluorescent photoproduct was detected using the clas­sical PIF method at λex/λem = 331/405 nm for DFB and 282/343 nm for FEN. In contrast, the DL­PIF method revealed the for­mation of three fluorescent photoproducts at λex/λem = 225/308 nm (PIF1), 280/342 nm (PIF2) and 295/420 nm (PIF3) for FEN (Fig. 1), and of two fluorescent photoproducts at λex/λem = 230/342 nm (PIF’1) and 220/422 nm (PIF’2) for DFB (Fig. 2) [1,2]. GC­MS allowed the identification of these photoproducts by comparing their fluorescence spectral characteristics (Fig. 3) to those of standard compounds (p­hydroxyaniline and phe­nol). The two later compounds are known to contribute to the photodegradation pathways of benzoyl­ and phenylurea pesti­cides. Indeed, both pesticides presented the same PIF photo­product at λem = 342 nm than p­hydroxyaniline. This fluores­cence emission wavelength might also correspond to the formation of 3­[4­(4­aminophenyl)] phenyl]­1,1­dimethylurea, taking place during the photo­rearrangement of benzoyl and phenylureas [5]. In the case of DL­PIF, it was found that the FEN PIF1 presented the same fluorescence characteristics than phenol (λem = 308 nm).

Conclusion

In this work, a simple, inexpensive, sensitive and precise PIF method has been developed for the determination of two benzo­yl­ and phenylurea pesticides, namely diflubenzuron and fenu­ron, in Senegal natural water samples. Classical PIF and DL­PIF methods were found to be of great analytical interest for moni­toring both pesticides in natural waters. Also, it can be conclud­ed that the combination of the PIF and DL­PIF methods and of GC­MS should be suitable to confirm the presence of both pesti­cides DFB and FEN and their photoproducts in natural waters, and to monitor their evolution in the environment.

Affiliations1 Equipe des Matériaux, Electrochimie et Photochimie Analyt­

iques, Université A. Diop, Bambey, Sénégal2 Laboratoire de Photochimie et d’Analyse, Univ. Cheikh. Anta

Diop, Dakar, Sénégal3 Laboratoire Géomatériaux et Environnement (LGE), Université Paris­Est Marne­la­Vallée, Paris, France

4 Laboratoire Optimag, EA 938, Faculté des Sciences, Université de Brest, Brest Cedex, France

ContactProf. Dr. Jean-Jacques AaronLaboratoire Géomatériaux et Environnement (LGE)Université Paris-Est Marne-La-Vallée Paris, [email protected]

Further articles on chromatography: https://bit.ly/WAS-Chromatography

Literatur: http://bit.ly/GLJ-Aaron

Fig. 3: Comparison of the PIF emission spectra of FEN and DFB photopro-ducts (PIF 1, 2 and 3) with the fluorescence emission spectra of the stan-dard compounds (phenol and p-hydroxyaniline) – Figure reprinted with authorization from reference [3].

Fig. 1: Evolution of the photoproducts DL-PIF emission spectra of a FEN aqueous solution (initial concentration = 2.5 μg mL-1) vs. the irradiation time. Laser beam: 240 nm, 1 mJ,10Hz – Figure reprinted with authorization from reference [2].

Fig. 2: Evolution of the excitation/emission fluorescent matrix with laser ir-radiation (at 240 nm) for DFB after 1 min of irradiation – Figure reprinted with authorization from reference [2].

38 Mass Spectrometry

Articles

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G.I.T. Laboratory Journal 1/2020

New, Robust Wide Pore Columns

The new YMC­Triart Bio C18 and the re­cently introduced YMC­Triart Bio C4 col­umns are innovative wide pore phases for RP­(U)HPLC. They are based on the mod­ern hybrid silica support from YMC. With a pore size of 300 Å and their specific selec­tivity, the YMC­Triart Bio columns provide the perfect solution for peptide and protein analysis as well as the analysis of antibod­ies and oligonucleotides. High flexibility in method development is available due to high temperature (up to 90°C) and pH sta­bility (Bio C18: pH 1­12; Bio C4: pH 1­10). The superior column­to­column and lot­to­lot reproducibility guarantees reliable re­sults in quality control for BioLC.

Wide Pore C18 Biocolumns

YMC­Triart Bio C18 columns are designed for the separation of proteins and pep­

tides with a medium MW e.g. Somatropin (22 kDa) or oligonucleotides. Due to the reduced hydrophobicity compared to con­ventional C18 phases, it is possible to achieve shorter analysis times for strong­ly retained substances when using these columns. Also, they are suitable for highly sensitive analytical and structural analy­sis of proteins using LC­MS, due to the ex­cellent peak shapes achieved using LC­MS compatible eluents.

Wide Pore C4 Biocolumns

As the YMC­Triart Bio C4 columns feature shorter ligands with reduced hydropho­bicity their ideal application is the analy­sis of large proteins. Specifically, intact antibodies can be analysed without re­strictions through use of the high temper­ature column stability. Furthermore, the columns are suitable for the analysis of very hydrophobic proteins and peptides

such as Amyloid β peptides, due to the re­duced hydrophobicity of the phase.

Product Options

Both YMC­Triart Bio phases are available with 1.9 µm particles for UHPLC together with 3 and 5 µm for HPLC separations. Op­tional, bioinert columns are available for especially sensitive separations as well as (semi)preparative YMC­Actus columns for high demanding preparative separations. YMC­Actus columns can be used at up to 300 bar and allow high sample throughput with long column lifetimes. To analyse very low sample amounts or to achieve high sensitivity, a variety of microLC and nanoLC dimensions are also available.

Innovative UHPLC/HPLC Solutions for BioLCNew UHPLC/HPLC phases for biomolecules such as peptides, proteins, antibodies or oligonucleotides need to possess a variety of demanding criteria. In particular these criteria include high temperature and pH stability, high resolution and MS compatibility. Characteristics such as robustness of the phase and excellent lot-to-lot reproducibility are of the highest priority for the use in quality control. To satisfy these requirements of BioLC users, YMC’s main focus is the production and provision of lasting reliable prod-ucts as well as the development of new and innovative phases.

YMC-Triart Bio C18 and Bio C4 columns are dedicated for the analysis of different biom-olecules: peptides/proteins, antibodies and oli-gonucleotides.

ContactDr. Daniel EßerProduct Manager Analytical ChromatographyYMC Europe GmbHDinslaken, [email protected]

Advertorial 39

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G.I.T. Laboratory Journal 1/2020

ParticleScout: Find, Classify and Identify MicroparticlesWITec’s ParticleScout is a soft­ware tool that accelerates and simplifies the workflow of comprehensive microparticle analysis. Confocal Raman mi­croscopy is used for label­free and non­destructive chemical characterization of the parti­cles.

In a white­light image of the sample, particles are au­

tomatically located and cate­gorized according to physical properties such as area or perimeter. A Raman spec­trum is then automatically acquired from each particle and used for chemical identi­fication. To this end, WITec’s TrueMatch Raman database software is seamlessly inte­grated with ParticleScout. Fi­

nally, a comprehensive report on the sample composition is generated. User­selectable fil­ters reveal correlations be­tween the physical and chem­ical properties of particles.

Key Features of ParticleScout:

▪ accelerated workflow ▪ automated large­area par­

ticle survey ▪ user­specified classifica­

tion by physical proper­ties

▪ targeted Raman spectral acquisition

▪ fully­integrated True­Match Raman database software

▪ quantitative summaries

ParticleScout is the perfect tool for thorough and de­tailed microparticle analyses

in microplastics or pharma­ceutical research, environ­mental or food science, and many other fields of applica­tion.

Analytica 2020: Hall A2 / booth 406

ContactWITec GmbHUlm, [email protected]

Support for Maintenance Processes Using RFID

RFID handhelds from Pepperl+Fuchs and ecom paired with custom software from Neoception allow for convenient and effi-cient maintenance, even in hazardous ar-eas. Safety is of the utmost importance during the transport of potentially danger-ous process media in chemical processing plants, and regular inspection and mainte-nance of hoses is required by law. RFID handhelds enable maintenance processes to be performed and documented effi-ciently. Each hose is clearly identifiable via a UHF RFID tag, which stores the equipment number, test date and time, the hose length and diameter, its conductivity, the test pressure, and the due date of the next inspection. The auditor is guided by the software through the maintenance procedure step-by-step and the results of the test can be seam-lessly transferred to a back-end system and serve as the documentation and proof of the tests having been carried out.

Pepperl+Fuchswww.pepperl-fuchs.com

Easily Mop Industrial Spills

Brady Corporation introduces the Handy-sorb, a new, practical tool to remove spills in many workplaces. The mop fea-tures an extendable handle and works with engineered pads to safely remove spills in a highly efficient and easy way. It uses inert meltblown polypropylene pads and pillows that do not react with any spilled substance. Its no-touch quick-re-lease system enables users to clean up a wide range of fluids without ever having to touch any of the removed substances. If necessary, the extendable handle can be used to keep a safe distance while re-moving a spill, when fully extended up to 1.83 metres, which also offers easier accessibility in difficult to reach areas. The pads used can absorb spills in sec-onds and absorbs up to 25 times its weight in spilled fluids to reduce waste disposal volume and cost.

Brady Corporationwww.bradycorp.com

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40 Products

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G.I.T. Laboratory Journal 1/2020

Digital Microscope Ensures Quality and Safety of ImplantsMedical aesthetics specialist GC Aesthetics turned to Keyence recently to acquire a new digital microscope. One particular cli-ent requirement was to inspect the sur-face finish on implants to assess vari-ous metrology parameters and surface topography features, patterns, consis-tency and uniformity. Techniques such as scanning electron microscopy-SEM and digital microscopy were previously out-sourced and time-consuming. The VHX-6000 series digital microscope was purchased to have the in-house capability to produce high resolution images of surface to-pographies along with the necessary metrology parameter specific data on sur-face profiles. High volumes of sample data on varied and multiple samples are now available in days, and in some cases hours, compared to before.

Keyencewww.keyence.co.uk

SWIR Camera

The Nirvana HS camera from Tele-dyne Princeton utilizes the advan-tages of the second near-infrared window (NIR-II) window to meet the increasingly diverse needs of today’s scientific, industrial, and medical communities. Building on the high-perfor-mance Nirvana LN (liquid nitro-gen cooled) and the Nirvana 640 (super-cooled), the Nirvana HS version combines speed, flexibility, perfor-mance and value. The HS runs at 250 frames per second in 16 bit mode and offers both integrate-then-read (ITR) and integrate-while-read (IWR) modes for low noise and high duty cycle. The advanced thermal design includes deep cooling to -55 °C and incorporates a vacuum sealed chamber. The man-ufacturer’s Lightfield software provides an intuitive interface and powerful analytical functions, eliminating the need for any third party hardware or software.

Teledyne Princeton Instrumentswww.princetoninstruments.com

Service to Facilitate Target Validation of Deubiquitylase (DUB) EnzymesUbiquigent has launched its DUB profiler-Cell service, which is part of its Drug Discovery Screening Platform. The service supports the development of novel deubiquitylase (DUB) enzyme inhibitors by revealing the active DUBome in a given cell-line or tissue (e.g. non-diseased and diseased), and by reporting the target engagement of test compounds to that active DUBome. By pre-incubat-ing lysates or live cells with test compounds, the assay can be used to evaluate target engagement by compounds. This is the company’s first assay service that incorporates the use of mass spectrometry (MS) to enable an unbiased readout of cellular compound-target engagement, with further new assays planned for launch later this year.

Ubiquigentwww.ubiquigent.com

Operator-Free GMP-Compliant Cell Sorting System

With its new MACS GMP Tyto Cartridge, Miltenyi Biotec opens new possibilities for flu-orescence-based, GMP-com-pliant cell sorting. Specifically developed for the MACS Quant Tyto Cell Sorter, the car-tridge offers GMP-compliant multiparametric, fluorescence-based cell sorting under safe and sterile conditions. The cartridge is already being put to use in the first hu-man iPS trial of Parkinson’s disease, currently taking place at CiRA, Kyoto, where iPS cells were first discovered just twelve years ago. The cell sorter provides high-speed, multiparameter cell sorting in a fully closed system. Cells remain inside the closed cartridge at all times during the sorting process, and no sheath fluid is present in the system, so cells only come in contact with GMP-compliant products.

Miltenyi Biotecwww.miltenyibiotec.com

Compressor-Free Plate Sealer

Thermo Fisher Scientific announces the launch of a next-generation, compressor-free plate sealer, designed to alleviate the mainte-nance burden on operators, while offering unparalleled process customization capabili-ties, intuitive operation and significant work efficiencies. Biotechnology, pharmaceutical and academic research laboratories can now benefit from a system capable of simplifying the plate sealing process and enabling im-proved reliability and productivity for both stand-alone, as well as, integrated robotic projects. As a fully electrically operated system, the Thermo Scientific ALPS 5000 has been developed to increase the efficiency of the heat plate sealing process and offers maximum flexibility to support high throughput applications. The system eliminates the need for te-dious and costly replacement of expensive vacuum cups, onboard compressors and compressed air lines, typically required when using conventional pneumati-cally driven equipment.

Thermo Fisher Scientificwww.thermofisher.com

Integrated Encoder for Flat Motors

The BXT motor family from Faulhaber, comprising brushless DC-motors with an especially short design, has been expanded with the diameter-complaint IEF3-4096 magnetic encoder. With just 6.2 mm of additional length, the motor/en-coder units also remain extremely short. The encoder is fully integrated in the robust motor housing. In this flat design, the IEF3-4096 offers three channels with index function and a high resolution of up to 4096 lines per revolution. A variant with line driver is also available with the IEF3-4096 L. The encoder can be combined with the 2214…BXT H, 3216…BXT H and 4221…BXT H housed BXT motors.

Faulhaberwww.faulhaber.com

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G.I.T. Laboratory Journal 1/2020

Extracellular Vesicles-Focused Analytical Services

Exosomics has implemented NanoFCM Nanoanalyzer instrument at its Siena, Italy, facility to offer sophisticated contract research and measurement services worldwide and has become an approved service supplier of nano-flow cytome-try measurements. These can be performed as stand-alone or coupled to solu-tions provided by Exosomics who will be able to supply a wide range of nano-flow cytometry measurements. The instrument will also allow the company to further develop its own liquid biopsy pipeline, which requires the highest level of accuracy and reproducibility. The Nanoanalyzer is designed For Research Use Only. It is not for use in diagnostic procedures.

Exosomicswww.exosomics.eu

Vacuum Pump with Long-Term Performance

In addition to the intelligent and easy control of vacuum in the laboratory, the PC 3001 Vario select from Vacuubrand has been spe-cially designed for reliability and long-term performance. The combination of variable-speed Vario chemistry diaphragm pump and the Vacuu Select vacuum controller give the pump long service intervals, high reliability and whisper-quiet operation. The combination of special fluoroplastics that are used in construction guarantees uncompromising chemical resistance while the extreme diaphragm life of the vacuum pump of typically 15,000 operating hours is significantly extended by the automatic speed adjustment of the motor. Diaphragm pumps require neither oil nor water and are therefore very environmentally friendly and particularly easy to clean. As a special campaign, Vacuubrand offer a warranty extension of three years for all PC 3001 Vario select pumping stations until 31st March 2020.

Vacuubrandwww.vacuubrand.com

Wells for Cost-Effective Cell Culture and High-Resolution MicroscopyWith its thin polymer cov-erslip bottom, the µ-Slide 18 Well from Ibidi enables excellent cell adhesion onto the tissue culture-treated surface. In addi-tion, it has a high optical quality and is ideally suit-ed for a variety of micro-scopic techniques such as widefield fluorescence, confocal microscopy and DIC. For scientists who perform special microscopic applications, such as TIRF and super-resolution microscopy, the company offers the µ-Slide 18 Well Glass Bottom with a #1.5H D 263 M Schott glass. It is also available as a sticky-Slide 18 Well without any bottom, which enables the researcher to mount any chosen substrate. The entire µ-Slide 18 Well family is ideal for experiments with small cell numbers and low reagent volumes. The spaces between the individual well walls minimize any well-to-well crosstalk and contamination. Scientists who would like to test one of the variations with their own experiments can request free samples.

Ibidiwww.ibidi.com

Solvent Removal Evaporators

SP Scientific has launched its HT Series 3i Evaporators, the updated replacements for the highly successful HT Series 3 evapora-tor range. They have been developed to be even more user and environment-friendly, while enabling enhanced evaporation and lyophilization results. Anti-bumping tech-nology prevents foaming and bumping to eliminate cross-contamination and sample loss. The high-performance dry vacuum pump, and F-Gas compliant -75 °C auto-defrost and draining condenser with R449A and R170 refrigerants, ensure fast, controlled evaporation of all sample types, yet with a low environmental im-pact and global warming potential. Simplified help screens, and improved pre-set routine and automatic programming, allow the most complex, multi-stage evapo-ration methods to be performed quickly and easily, even by occasional users.

SP Scientificwww.spscientific.com

Visible/SWIR Machine Vision and Microscopy Camera

Princeton Infrared Technologies recently launched its compact MV Cam series short-wave-infrared (SWIR) and visible camera that supports a high frame rate at mega-pixel resolution with no ITAR restrictions. The megapixel indium gallium arsenide (In-GaAs) camera provides 1280 x 1024 reso-lution SWIR imagery at up to 95 frames per second, with higher frame rates for user-selectable regions of interest. At 12 µm pixel pitch, the MV Cam InGaAs image sensor yields extremely low dark current and high quantum efficiency, providing sensitivity across the SWIR and visible wavelength bands from 0.4 to 1.7 µm. The standard camera configuration uses a single-stage thermoelectric cooler with no moving parts, integrated in a sealed package to stabilize the image sensor at 20 °C. MV Cam’s advanced digital array (PIRT1280A1-12) generates 14-bit digital image data with no image lag and read noise less than 45 e-.

Princeton Infrared Technologieswww.princetonirtech.com

Improved Image Analysis Method for Steel Quality ControlThe release of Olympus Stream image analysis software version 2.4.2 in-cludes an improved image analysis so-lution to measure and rate non-metal-lic inclusion content in high-purity steel. The mode detects and classifies individual fields on a large scan area, expanding on the software’s capabilities to detect and classify individual inclusions in worst field mode and providing statistical results of inclusion content on the entire scan area ac-cording to three international standards: ASTM E45-18 (method D), ISO 4967:2013 (method B) and EN 10247:2017 (method K). The new version also optimizes Olympus Stream software for the Schott Visiled controller MC 1500, enabling users to easily control LED ring light illumination on their stereo micro-scopes and remove halation caused by LED reflection on the sample surface.

Olympuswww.olympus-ims.com

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Bio-Rad Laboratories 16

Brady Corporation 40

Celisca Center for Life Science Automation 26

Dr. Fritz Faulhaber 41

Empa – Materials Science and Technology 28

Exosomics 42

Hahnemühle Fineart 3

Ibidi 42

Institut für Energie- und Umwelttechnik (IUTA) 24

Keyence UK 41

Metrohm Titelseite, 12

Miltenyi Biotec 41

Olympus 42

Paperless Lab Academy 6

Pepperl+Fuchs 40

Polyscience 19, 21, 23, 25, 27, 29

Princeton Instruments 42

Shimadzu Europa 18, 4. US

Socorex Isba 33

SP Scientific 42

The Francis Crick Institute 14

Thermo Fisher Scientific 41

Ubiquigent 41

Universidad Nacional de Rosario 20

Université Paris-Est Marne-La-Vallée 36

University of Michigan 9

University of Utrecht 32

Vacuubrand 42

Witec 40

YMC Europe 37, 39

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Surfing different wavesApplying different wavelengths, the compact UV-2600/2700i series of research grade UV-VIS spectrophotometers enables high-precision spectral analysis. It is based on the Shimadzu LO-RAY-LIGH® diffraction gratings optical system and covers a wide range of applications such as organic and inorganic compounds, biological samples, optical materials and photovoltaics. User-friendly economic design with smallest footprint in its class, energy saving electronics, comprehensive system control soft-ware including validation, USB connection as well as the widest scope of accessories

Wide wavelength range of up to 1,400 nm enables expanded research of photovoltaics with UV-2600i High absorbance level of the UV-2700i double monochromator optics allows measurement of high density samples up to 8 Abs. Ultra low stray light Shimadzu gratings offering “best in class” performance

www.shimadzu.eu /uv-2600i-uv-2700i

UV-2700i – UV-VIS Spectrophotometer

Shimadzu_GIT_032020.qxp_A4 25.02.20 15:28 Seite 1


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