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Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
2
INDEX
1. Hematoxylin stains ................................... 4
2. Eosin ................................... 6
3. Staining solutions for histology and cytology ................................... 8
4. Reagents I ................................... 48
5. Reagents II ................................... 55
6. Decalcifying solutions ................................... 58
7. Fixatives ................................... 59
8. Formalins ................................... 59
9. Solvents ................................... 60
10. Mounting and embedding media ................................... 60
11. Legend ................................... 61
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
3
Dear distributors, suppliers and customers,
We are pleased to present our new catalog “Microscopic stains and reagents for cytology, histology and microbiology”
Emmonya Biotech
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
4
1. Hematoxylin stains
Products Code Size DescriptionGill’s hematoxylin I 111601350 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei.111601310 1lGill’s hematoxylin II 111601450 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei.111601410 1lGill’s hematoxylin III 111601550 500ml For standard haematoxylin-eosin
staining of tissue sections. For nuclei111601510 1l
Gill I Gill II Gill III
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
5
Mayer’s hematoxylin 111600250 500ml For standard haematoxylin-eosin staining of tissue sections. For nuclei111600210 1l
Harris’ hematoxylin 111600450 500ml For standard haematoxylin-eosin staining of tissue sections. For nuclei111600410 1l
Weigert’s Hematoxylin 130880100 100 test For trichrome stains130880250 250ml130880500 500ml130880010 1l
Harris Mayer Weigert
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
6
2. Eosin
Products Code Size DescriptionEosin Y 1% aqueous sol. 111000050 50ml Standard haematoxylin-eosin staining
for cytoplasm111000300 300ml111000250 500ml111000210 1l
Eosin Y 5% aqueous sol. 111000050.5 50ml Standard haematoxylin-eosin staining for cytoplasm111000250.5 500ml
111000210.5 1l
Eosin Y 1% aqueous sol. Eosin Y 5% aqueous sol.
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
7
Eosin Y 0.5% alcoholic sol. 111000305 50ml Standard haematoxylin-eosin staining for cytoplasm111000325 250ml
111000350 500ml111000310 1l
Eosin-Phloxin B alcoholic sol. 111002005 50ml Standard haematoxylin-eosin staining for cytoplasm111002025 250ml
111002050 500ml111002010 1l
Eosin Y 0,5% alcoholic sol. Eosin-Phloxin B alcoholic sol.
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
8
3. Staining solutions for histology and cytology
Products Code Size DescriptionAlcian blue pH1 162802050 100test For demonstration of acid mucins in
tissue sections162802250 250ml162802500 500ml162802100 1l
Alcian blue pH2.5 161802050 100test For demonstration of carboxylated and some weakly sulfated mucins in tissue sections
161802250 250ml161802500 500ml161802100 1l
Alcian blue pH1 - P.A.S. 111200100.1 100test For neutral and acid mucins in tissue sections111200101.1 100ml
111200250.1 250ml111200500.1 500ml111200010.1 1l
Alcian blue pH2.5 - P.A.S. 111200100 100test For neutral and acid mucins in tissue sections111200101 100ml
111200250 250ml111200500 500ml111200010 1l
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
9
Alcian Yellow - Toluidine Blue 111300100 100test For screening patient specimens for Helicobacter pilory111300250 250ml
Azan Trichrome 121111100 100test Method for connective tissue, especially to show muscle fibers, collagen, reticulum, kidney glomerular stroma in tissue sections
121111250 250 ml121111010 1l
Brilliant Cresyl Blue for reticulocytes
111400050 100test Blood smears examination for reticulocytes111400125 125ml
111400250 250ml111400500 500ml111401000 1l
Bennhold’s Congo red for amyloid 131003050 100test Stain for amyloid deposits in tissues131003250 250ml131003500 500ml131003100 1l
Highman’s Congo red for amyloid 131103205 100test Stain for amyloid deposits in tissues131103250 250ml131103500 500ml131103100 1l
CytoMax - fast Papanicolau for cyto PAP smear
221222050 100test For cyto PAP and other smears (cytological specimens)221222100 100ml
221222250 250ml221222500 500ml
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
10
Giemsa stain 111120100 100test Giemsa concentrate solution111120250 250ml111120500 500ml111120010 1l
Gomori silver impregnation 114080100 100test Method to show argyrophilic reticular fibers in connective tissue
Gram for microbiology 100802100 100test Differentiation between Gram positive and Gram negative bacteria in different specimens
100802125 100ml100802250 250ml100802500 500ml100802010 1l
Grocott’s methenamine silver nitrate method for fungi
143823100 100test To identify fungi in tissue section143823010 100ml
Haematocolor (hematocolor) 111316050 100test Blood smear examinations111316100 100ml111316250 250ml111316500 500ml
Leishman stain 113273100 100test Blood smear examinations113273125 125ml113273250 250ml113273500 500ml
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
11
Leishman-Wright stain 113273100W 100test Blood smear examinations113273125W 125ml113273250W 250ml113273500W 500ml
Mallory trichrome 120802100 100test Staining solution for connective tissueMasson trichrome Goldner 110802100.1 100test Staining solution for connective
tissue - collagen and muscle fiberMasson trichrome Standard 110802100 100test Staining solution for connective
tissue - collagen and muscle fiberMay Grunwald 111200100 100test Fixative for MGG blood smear
examinations111200250 250ml111200500 500ml111200010 1l
May Grunwald - Giemsa 118080100 100test Blood smear examination118080010 1l
Methenamine Silver Impregnation (P.A.S.M.)
114382100 100test Kit for histology (lamina basalae, argyrophil cells etc.).
Methylene Blue Loffler 112000100 100test Alkaline stain for cell nuclei in smears from different origin and pathogens112000250 250ml
112000500 500ml112000010 1l
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
12
New Methylene Blue for reticulocytes
1114003050 100test Blood smear examination for reticulocytes1114003125 125ml
1114003250 250mlOil Red O for lipids in frozen sections
122092100 100test Lipid staining in frozen sections 122092125 125ml122092250 250ml
Orcein 155802100 100test To demonstrate elastic fibers in tissue sections, recomended for vascular pathology
155802250 250ml
Papanicolau - OG6 112220250 250ml Stain solution I for cyto PAP smears112220500 500ml112220010 1l
Papanicolau - EA65 112230250 250ml Stain solution II for cyto PAP smears112230500 500ml112230010 1l
Papanicolau - EA50 112230250.1 250ml Stain solution II for cyto PAP smears112230500.1 500ml11223010.1 1l
Periodic Acid Schiff (P.A.S.) 113080100 100test Examination of mucosubstances (mucopolysaccharides), lamina basalae, glycogen deposits in liver etc. in tissue sections
113080101 100ml113080250 250ml113080500 500ml
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
13
Rapihem 111317050 100test Blood smears, other cytological specimens and fine needle aspirations examination
111317250 250ml111317500 500ml111317100 1l
Sudan III Propylene Glycol 112700050 100test Triglyceride staining in frozen sections112700250 250ml112700500 500ml112700100 1l
Sudan III alcohol solution 112700050A 100test Staining of triglycerides in frozen tissue sections.112700250A 250ml
112700500A 500ml112700100A 1l
Toluidine Blue stain for hystology 123001050 100test To demonstrate metachromatic substances and mast cells in tissue sections
123001250 250ml123001500 500ml123001100 1l
Van Gieson Trichrome stain 130802100 100test Staining for connective tissue130802125 125ml130802250 250ml130802500 500ml
Weigert for elastic fibers 124001100 100test To demonstrate elastic fibers in tissue sections124001125 125ml
Full instructions for use on www.emmonya.comCopyright Emmonya Biotech Ltd. ©
14
Wright’s Stain for cytology 113273100WP 100test Fine needle aspirations and other cytological specimens examination113273125WP 125ml
113273250WP 250ml113273500WP 500ml
Ziehl Neelsen cold 116450050 100test Microscopic examination of Mycobacteria116450250 250ml
116450500 500ml
15
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
ALC
IAN
BLU
E pH
1
num
ber o
f tes
ts10
0pr
oced
ure
time
50 m
in. (
20 m
in. m
icro
wav
e ov
en m
odifi
catio
n)pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Wea
kly
and
stro
ngly
sul
fate
d ac
id m
ucin
sB
lue
Nuc
lei
Red
to p
ink
Oth
er ti
ssue
ele
men
tsP
ale
pink
A
lcia
n bl
ue is
use
d fo
r the
dem
onst
ratio
n of
aci
d m
ucin
s, w
hich
can
be
pro-
duce
d by
sev
eral
type
s of
epi
thel
ial a
nd c
onne
ctiv
e tis
sue
tum
ors.
Aci
d m
ucin
s al
so
play
role
in c
olla
gen
dise
ases
and
ear
ly s
tage
s of
ath
eros
cler
osis
.
Rea
gent
s:
A –
Alc
ian
blue
sta
inin
g so
lutio
n рН
1B
– N
ucle
ar F
ast R
ed s
tain
ing
solu
tion
Alc
ian
blue
pH
1 re
actio
n - i
ntes
tine
16
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
ALC
IAN
BLU
E pH
2,5
num
ber o
f tes
ts10
0pr
oced
ure
time
~50
min
. (20
min
. mic
row
ave
oven
mod
ifica
tion)
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, in
glas
s da
rk
bottl
e, ti
ghtly
clo
sed
Car
boxy
late
d an
d so
me
wea
kly
sulfa
ted
acid
muc
ins
Blu
e
Nuc
lei
Red
to p
ink
Oth
er ti
ssue
ele
men
tsP
ale
pink
A
lcia
n bl
ue is
use
d fo
r the
dem
onst
ratio
n of
aci
d m
ucin
s, w
hich
can
be
pro-
duce
d by
sev
eral
type
s of
epi
thel
ial a
nd c
onne
ctiv
e tis
sue
tum
ors.
Aci
d m
ucin
s al
so
play
role
in c
olla
gen
dise
ases
and
ear
ly s
tage
s of
ath
eros
cler
osis
.
Rea
gent
s:
A –
Alc
ian
blue
sta
inin
g so
lutio
n рН
2,5
B –
Nuc
lear
Fas
t Red
sta
inin
g so
lutio
n
Alc
ian
blue
pH
2,5
reac
tion
- int
estin
e
17
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
ALC
IAN
BLU
E pH
1 -
P.A
.S.
num
ber o
f tes
ts10
0pr
oced
ure
time
~50
min
. (20
min
. mic
row
ave
oven
mod
ifica
tion)
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Wea
kly
and
stro
ngly
sul
fate
d ac
id m
ucin
sB
lue
Neu
tral m
ucin
sM
agen
taM
ixtu
res
of a
cid
and
neut
ral m
ucin
sB
luis
h to
redd
ish
purp
leN
ucle
i (if
cont
ra-s
tain
ed)
Blu
e to
bla
ck
A
lcia
n bl
ue a
nd P
AS
reac
tions
can
be
com
bine
d to
dem
onst
rate
bot
h ne
utra
l an
d ac
id m
ucin
s in
tiss
ue s
ectio
ns.
Rea
gent
s:
A –
Alc
ian
blue
sta
inin
g so
lutio
n рН
1C
– S
chiff
Rea
gent
B –
Per
iodi
c ac
id s
olut
ion
Alc
ian
blue
pH
1-PA
S re
actio
n - i
ntes
tine
18
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
ALC
IAN
BLU
E pH
2,5
- P.
A.S
.
num
ber o
f tes
ts10
0pr
oced
ure
time
~50
min
. (20
min
. mic
row
ave
oven
mod
ifica
tion)
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, in
glas
s da
rk
bottl
e, ti
ghtly
clo
sed
Car
boxy
late
d an
d so
me
fain
tly a
cid
sulfo
muc
ins
Blu
e
Neu
tral m
ucin
sM
agen
taM
ixtu
res
of a
cid
and
neut
ral m
ucin
sB
luis
h to
redd
ish
purp
leN
ucle
i (if
cont
ra-s
tain
ed)
Blu
e to
bla
ck
A
lcia
n bl
ue a
nd P
AS
reac
tions
can
be
com
bine
d to
dem
onst
rate
bot
h ne
utra
l an
d ac
id m
ucin
s in
tiss
ue s
ectio
ns.
Rea
gent
s:
A –
Alc
ian
blue
sta
inin
g so
lutio
n рН
2,5
C –
Sch
iff R
eage
ntB
– P
erio
dic
acid
sol
utio
n
Alc
ian
blue
pH
2,5
reac
tion
- int
estin
e
19
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inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
ALC
IAN
YEL
LOW
-TO
LUID
INE
BLU
E
num
ber o
f tes
ts10
0pr
oced
ure
time
~25
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Hel
icob
acte
r pilo
ryD
ark
blue
Muc
ins
Yello
wB
ackg
roun
dB
lue
scal
e
S
tain
ing
solu
tion
for s
cree
ning
pat
ient
spe
cim
ens
for H
elic
obac
ter p
ilory
–
spira
l sha
ped
bact
eriu
m im
plic
ated
in g
astri
c in
flam
mat
ion,
pep
tic u
lcer
s an
d ga
stric
ca
ncer
.
Rea
gent
s:
А –
Per
iodi
c ac
idС
– A
lcia
n ye
llow
sta
inin
g so
lutio
nЕ
– S
odiu
m h
ydro
xide
sol
utio
n
В –
Sod
ium
bis
ulfit
e so
lutio
nD
– T
olui
dine
blu
e st
aini
ng s
olut
ion
Alc
ian
yello
w-T
olui
dine
blu
e re
actio
n - i
ntes
tine
20
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
AZA
N T
RIC
HR
OM
E
num
ber o
f tes
ts10
0pr
oced
ure
time
~100
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Col
lage
n, re
ticul
um, a
nd k
idne
y gl
omer
u-la
r stro
ma
Dee
p bl
ue
Mus
cle
Ora
nge
Ery
thro
cyte
s R
ed
R
ecom
men
ded
met
hod
for c
onne
ctiv
e tis
sue,
esp
ecia
lly to
sho
w m
uscl
e fib
ers,
col
lage
n, re
ticul
um, k
idne
y gl
omer
ular
stro
ma
in ti
ssue
sec
tions
.
Rea
gent
s:
А –
Azo
carm
ine
solu
tion
C –
Diff
eren
tiatio
n so
lutio
nE
– S
tain
ing
solu
tion
В –
Ani
line
solu
tion
D –
Pho
spho
mol
ybdi
c ac
id s
olut
ion
Aza
n tri
chro
me
stai
ning
: A) k
idne
y; B
)ute
rus
AB
21
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
BR
ILLI
AN
T C
RES
YL B
LUE
num
ber o
f tes
ts10
0pr
oced
ure
time
~35
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Ret
icul
ocyt
esG
reen
ish
to b
lue
with
dar
k bl
ue ri
bonu
-cl
eopr
otei
n gr
anul
es in
side
Ery
thro
cyte
sG
reen
ish
to b
lue
B
lood
sm
ears
exa
min
atio
n fo
r ret
icul
ocyt
es. B
rillia
nt c
resy
l blu
e is
mor
e us
ed
in h
emat
olog
y la
bora
torie
s th
an in
his
tolo
gy la
bora
torie
s. It
is in
valu
able
for t
he s
tain
ing
of re
ticul
ocyt
es w
here
as it
s us
e in
his
tolo
gy is
lim
ited
to v
ital s
tain
ing.
Rea
gent
s:
Bril
liant
Cre
syl B
lue
phys
iolo
gica
l sol
utio
n
Bril
liant
Cre
syl B
lue
- blo
od s
mea
r
22
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
BEN
NH
OLD
’S C
ON
GO
RED
num
ber o
f tes
ts10
0pr
oced
ure
time
~90
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Am
yloi
dP
ink
to re
d (a
pple
-gre
en b
irefri
ngen
ce
with
pol
ariz
ed li
ght)
Nuc
lei
Blu
e
S
tain
for a
myl
oid
depo
sits
in ti
ssue
s. A
ppro
pria
te fo
r tis
sue
sect
ions
from
5-
12μm
. Am
yloi
d de
posi
ts a
re fo
rmed
in ti
ssue
s sp
onta
neou
sly
or in
ass
ocia
tion
with
a
wid
e ra
nge
of d
isea
ses.
In c
ases
of s
uspe
cted
am
yloi
dosi
s, th
ick
sect
ions
at 1
2 μm
m
ay b
e be
nefic
ial i
n de
mon
stra
ting
min
ute
depo
sits
of a
myl
oid.
Rea
gent
s:
A –
Con
go re
d st
aini
ng s
olut
ion
acco
rdin
g B
enho
ldC
– M
ayer
’s h
aem
atox
ylin
sol
utio
n
B –
Sod
ium
hyd
roxi
de s
olut
ion
Ben
nhol
d’s
cong
o re
d fo
r am
yloi
d - k
idne
y
23
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
HIG
HM
AN
’S C
ON
GO
RED
num
ber o
f tes
ts10
0pr
oced
ure
time
~20
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Am
yloi
dP
ink
to re
d (a
pple
-gre
en b
irefri
ngen
ce
with
pol
ariz
ed li
ght)
Nuc
lei
Blu
e if
cont
ra-s
tain
ed
S
tain
for a
myl
oid
depo
sits
in ti
ssue
s. S
uita
ble
for p
araf
fin s
ectio
ns fi
xed
in
10%
form
alin
. Am
yloi
d de
posi
ts a
re fo
rmed
in ti
ssue
s sp
onta
neou
sly
or in
ass
ocia
tion
with
a w
ide
rang
e of
dis
ease
s. In
cas
es o
f sus
pect
ed a
myl
oido
sis,
thic
k se
ctio
ns a
t 12
μm m
ay b
e be
nefic
ial i
n de
mon
stra
ting
min
ute
depo
sits
of a
myl
oid.
Rea
gent
s:
A- C
ongo
red
stai
ning
sol
utio
n ac
cord
ing
Hig
hman
B- P
otas
sium
hyd
roxi
de s
olut
ion
Hig
hman
’s c
ongo
red
for a
myl
oid
- kin
dey
24
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
CYT
OM
AX
num
ber o
f tes
ts10
0pr
oced
ure
time
3 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Nuc
lei
Viol
et-b
lue
to b
lack
Cyt
opla
sm (e
osin
ophi
lic)
Pin
kC
ytop
lasm
(bas
ophi
lic)
Turq
uois
e to
blu
eC
ytop
lasm
(met
apla
sia
cells
)W
ith b
oth
blue
and
pin
kE
ritro
cyte
sR
edM
icro
orga
nism
sB
lue-
viol
et
S
tain
sol
utio
n fo
r cyt
o PA
P an
d ot
her s
mea
rs (c
ytol
ogic
al s
peci
men
s).
Rea
gent
s:
А –
Har
ris`s
hae
mat
oxyl
in s
olut
ion
C –
Mod
ified
pol
ychr
omic
EA
65 s
olut
ion
B –
2-P
ropa
nol A
bsol
ute
D –
80%
2-P
ropa
nol
Cyt
omax
- cy
to P
AP
smea
r
25
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
GIE
MSA
STA
IN
num
ber o
f tes
ts10
0pr
oced
ure
time
~40
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Lym
phoc
ytes
Ligh
t blu
e cy
topl
asm
Mon
ocyt
esU
sha
ped
nucl
eus
Gra
nulo
cyte
sN
eutro
phils
(3-6
lobe
d nu
cleu
s), e
osin
ophi
ls (2
lobe
d nu
-cl
eus)
, bas
ophi
ls (w
ith d
ark
viol
et to
bla
ck g
ranu
les)
Pla
tele
tsVi
olet
Erit
rocy
tes
Ligh
t red
Blo
od p
aras
ites
Red
B
lood
sm
ears
exa
min
atio
n.
Rea
gent
s:
Gie
msa
con
cent
rate
sol
utio
n
Gie
msa
sta
in -
bloo
d sm
ear
26
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
GO
MO
RI S
ILVE
R IM
PREG
NAT
ION
Gom
ori s
ilver
impr
egna
tion
- kid
ney
num
ber o
f tes
ts10
0pr
oced
ure
time
~30
min
.pr
oduc
t val
idity
1 ye
ar (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15-2
5°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, i
n gl
ass
dark
bot
tle,
tight
ly c
lose
d
Ret
icul
ar fi
bers
and
ner
vous
cel
ls
Bla
ckC
onne
ctiv
e tis
sue
Toba
cco
brow
nC
olla
gen
Gol
d ye
llow
R
ecom
men
ded
met
hod
to s
how
arg
yrop
hilic
retic
ular
fibe
rs in
con
nect
ive
tis-
sue,
esp
ecia
lly to
diff
eren
tiate
col
lage
n fib
ers
from
con
nect
ivum
.
Rea
gent
s:
А –
Pot
assi
um p
erm
anga
nate
sol
utio
nС
– O
xalic
aci
d so
lutio
nE
– A
mm
onia
cal s
ilver
sol
utio
nG
– S
odiu
m h
ypos
ulph
ite s
olut
ion
В –
Aci
d ac
tivat
ing
buffe
rD
– F
erric
am
mon
ium
sul
fate
sol
utio
n F
– N
eutra
l for
mal
in s
olut
ion
27
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
GR
AM
num
ber o
f tes
ts10
0pr
oced
ure
time
5 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Gra
m p
ositi
ve m
icro
orga
nism
sB
lue
Gra
m n
egat
ive
mic
roor
gani
sms
Red
D
iffer
entia
tion
betw
een
Gra
m p
ositi
ve a
nd G
ram
neg
ativ
e ba
cter
ia in
diff
eren
t sp
ecim
ens.
Rea
gent
s:
A –
Cry
stal
Vio
let s
olut
ion
C –
Dec
olor
isin
g so
lutio
nB
– L
ugol
’s s
olut
ion
for G
ram
D –
Saf
rani
n O
alc
ohol
ic s
olut
ion
Gra
m s
tain
ing
- bac
teria
l spe
cim
ents
28
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
GR
OC
OTT
for f
ungi
Gro
cott’
s m
etho
d fo
r fun
gi -
gast
ric ti
ssue
num
ber o
f tes
ts10
0pr
oced
ure
time
~100
min
. (~2
5 m
in. –
mic
row
ave
oven
mod
ifica
tion)
prod
uct v
alid
ity1,
5 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s4°
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Fung
iB
lack
Muc
in
Gre
yB
ackg
roun
d G
reen
To
iden
tify
fung
i in
tissu
e se
ctio
ns. T
he m
ucop
olys
acch
arid
e co
mpo
nent
s of
th
e fu
ngal
cel
l wal
l are
oxi
dize
d to
rele
ase
alde
hyde
gro
ups.
The
ald
ehyd
e gr
oups
then
re
act w
ith th
e si
lver
nitr
ate,
redu
cing
it to
a m
etal
lic s
ilver
, ren
derin
g th
em v
isib
le.
Rea
gent
s:
А –
Chr
omic
aci
d so
lutio
nС
– M
ethe
nam
ine
solu
tion
E –
Bor
ax w
ater
sol
utio
nG
– S
odiu
m th
iosu
lfate
sol
utio
n
В –
Sod
ium
hyd
roge
nsul
fite
solu
tion
D –
Silv
er n
itrat
e so
lutio
nF
– G
old
chlo
ride
wat
er s
olut
ion
H –
Lig
ht G
reen
sol
utio
n
29
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
HA
EMAT
OC
OLO
R (H
EMAT
OC
OLO
R)
num
ber o
f tes
ts10
0pr
oced
ure
time
1 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
B
lood
sm
ears
exa
min
atio
n.
Rea
gent
s:
A –
Met
hano
l abs
olut
eC
– H
aem
atoc
olor
Sol
utio
n II
B –
Hae
mat
ocol
or S
olut
ion
ID
– H
aem
atoc
olor
Pho
spha
te b
uffe
r
Lym
phoc
ytes
Blu
ish
Mon
ocyt
esU
sha
ped
nucl
eus
Gra
nulo
cyte
sN
eutro
phils
(3-6
lobe
d nu
cleu
s), e
osin
ophi
ls (2
lobe
d nu
-cl
eus)
, bas
ophi
ls (d
ark
stai
ned
due
to th
e la
rge
hist
amin
e gr
anul
es)
Pla
tele
tsP
urpl
eE
ritro
cyte
sP
urpl
e-pi
nkis
h
Hae
mat
ocol
or -
bloo
d sm
ear
30
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
LEIS
HM
AN
STA
IN
num
ber o
f tes
ts10
0pr
oced
ure
time
7 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
B
lood
sm
ears
exa
min
atio
n.
B –
Buf
fere
d w
ater
Rea
gent
s:
A –
Leis
hman
sta
inin
g so
lutio
n
Leis
hman
sta
in -
bloo
d sm
ear
Lym
phoc
ytes
Blu
ish
Mon
ocyt
esU
sha
ped
nucl
eus
Gra
nulo
cyte
sN
eutro
phils
(3-6
lobe
d nu
cleu
s), e
osin
ophi
ls (2
lobe
d nu
-cl
eus)
, bas
ophi
ls (d
ark
stai
ned
due
to th
e la
rge
hist
amin
e gr
anul
es)
Pla
tele
tsP
ink-
viol
etE
ritro
cyte
sP
ink
31
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
LEIS
HM
AN
-WR
IGH
T ST
AIN
num
ber o
f tes
ts10
0pr
oced
ure
time
7 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
B
lood
sm
ears
exa
min
atio
n.
B –
Buf
fere
d w
ater
Rea
gent
s:
A –
Leis
hman
-Wrig
ht s
tain
ing
solu
tion
Leis
hman
-Wrig
ht s
tain
- bl
ood
smea
r
Lym
phoc
ytes
Blu
ish
Mon
ocyt
esU
sha
ped
nucl
eus
Gra
nulo
cyte
sN
eutro
phils
(3-6
lobe
d nu
cleu
s), e
osin
ophi
ls (2
lobe
d nu
-cl
eus)
, bas
ophi
ls (d
ark
stai
ned
due
to th
e la
rge
hist
amin
e gr
anul
es)
Pla
tele
tsP
ink-
viol
etE
ritro
cyte
sP
ink
32
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MA
LLO
RY T
RIC
HR
OM
E
num
ber o
f tes
ts10
0pr
oced
ure
time
~20
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Nuc
lei
Red
Ery
thro
cyte
s O
rang
e re
dM
uscl
e R
edC
olla
gen
Blu
e
S
tain
ing
solu
tion
for c
onne
ctiv
e tis
sue.
Rea
gent
s:
А –
Aci
d fu
chsi
n st
aini
ng s
olut
ion
C –
Mal
lory
’s s
tain
ing
solu
tion
B –
Pho
spho
mol
ybdi
c ac
id s
olut
ion
Mal
lory
tric
hrom
e - p
lace
nta
33
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MA
SSO
N T
RIC
HR
OM
E ST
AN
DA
RD
num
ber o
f tes
ts10
0pr
oced
ure
time
~40
min
(+/-
pre-
fixat
ion
time)
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Nuc
lei
Bla
ckM
uscl
e fib
ers,
cyt
opla
sm, e
ryth
rocy
tes
Red
Col
lage
n, m
ucus
Blu
e
S
tain
ing
solu
tion
for h
isto
logy
. Col
lage
n an
d m
uscl
e fib
er s
tain
diff
eren
tially
w
hen
treat
ed s
eque
ntia
lly w
ith A
cid
Fuch
sin,
PTA
/PM
A an
d A
nilin
e B
lue.
Rea
gent
s:
A- W
eige
rt’s
Hae
mat
oxyl
in s
olut
ion
AC
- Aci
d fu
chsi
n st
aini
ng s
olut
ion
E- P
hosp
hotu
ngst
ic a
cid
solu
tion
G- A
cetic
aci
d so
lutio
n
B- W
eige
rt’s
Hae
mat
oxyl
in s
olut
ion
BD
- Pho
spho
mol
ybdi
c ac
id s
olut
ion
F- M
asso
n’s
anili
ne b
lue
solu
tion
Mas
son
trich
rom
e st
anda
rd -
uter
us
34
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MA
SSO
N T
RIC
HR
OM
E G
OLD
NER
Mas
son
trich
rom
e G
oldn
er -
uter
us
num
ber o
f tes
ts10
0pr
oced
ure
time
~40
min
. (+/
- pre
-fixa
tion
time)
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
S
tain
ing
solu
tion
for h
isto
logy
. Col
lage
n an
d m
uscl
e fib
er s
tain
diff
eren
tially
w
hen
treat
ed s
eque
ntia
lly w
ith A
cid
Fuch
sin,
PTA
and
Lig
ht g
reen
.
Rea
gent
s:
A- W
eige
rt’s
Hae
mat
oxyl
in s
olut
ion
AC
- Aci
d fu
chsi
n st
aini
ng s
olut
ion
E- L
ight
gre
en s
tain
ing
solu
tion
Nuc
lei
Bla
ckM
uscl
e fib
ers,
cyt
opla
sm, e
ryth
rocy
tes
Red
Col
lage
nG
reen
ish
B- W
eige
rt’s
Hae
mat
oxyl
in s
olut
ion
BD
- Pho
spho
tung
stic
aci
d so
lutio
n
35
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MAY
GR
UN
WA
LD S
TAIN
num
ber o
f tes
ts10
0pr
oced
ure
time
~2 m
in. (
+ G
iem
sa c
o-st
aini
ng)
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
S
uppl
emen
tary
sta
in fo
r May
Gru
nwal
d –
Gie
msa
blo
od s
mea
r exa
min
atio
n.
Rea
gent
s:
May
- G
runw
ald
Sta
in s
olut
ion
Whi
te b
lood
cel
ls
Fain
tly s
hape
dE
ryth
rocy
tes
Pin
kish
-red
May
Gru
nwal
d st
ain
- blo
od s
mea
r
May
Gru
nwal
d st
ain
only
May
Gru
nwal
d - G
iem
sa
36
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MAY
GR
UN
WA
LD -
GIE
MSA
num
ber o
f tes
ts10
0pr
oced
ure
time
~10-
35 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
B
lood
sm
ear e
xam
inat
ion
and
cyto
diag
nosi
s.
Rea
gent
s:
A –
Met
hano
l abs
olut
eC
– G
iem
sa s
tain
ing
solu
tion
Lym
phoc
ytes
Ligh
t blu
e cy
topl
asm
Mon
ocyt
esU
sha
ped
nucl
eus
Gra
nulo
cyte
sN
eutro
phils
(3-6
lobe
d nu
cleu
s), e
osin
ophi
ls (2
lobe
d nu
-cl
eus)
, bas
ophi
ls (w
ith d
ark
viol
et to
bla
ck g
ranu
les)
Pla
tele
tsVi
olet
Erit
rocy
tes
Red
dish
bro
wn
Blo
od p
aras
ites
Red
B –
May
-Gru
nwal
d st
aini
ng s
olut
ion
D –
Sor
enso
n bu
ffer p
H7
May
Gru
nwal
d G
iem
sa -
bloo
d sm
ear
37
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MET
HEN
AM
INE
SILV
ER IM
PREG
NAT
ION
- P.
A.S
.M.
P.A
.S.M
. - k
idne
y
num
ber o
f tes
ts10
0pr
oced
ure
time
~2 h
.pr
oduc
t val
idity
1 ye
ar (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
4 °C
, in
dark
gla
ss b
ottle
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht,
tight
ly c
lose
d
K
it fo
r his
tolo
gy (l
amin
a ba
sala
e, a
rgyr
ophi
l cel
ls e
tc.).
Rea
gent
s:
А –
Per
iodi
c ac
id s
olut
ion
C –
Hex
amet
hyle
ne te
traam
ine
solu
tion
E –
Gol
d ch
lorid
e so
lutio
n
Lam
ina
basa
lae
Bla
ckN
ucle
i and
bac
kgro
und
Pin
kish
-red
and
gre
en if
con
tra-s
tain
edP
ositi
ve c
ontro
lS
kin,
kyd
ney
В –
Silv
er n
itrat
e so
lutio
nD
– S
odiu
m te
trabo
rate
sol
utio
nF
– Fi
xativ
e
38
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
MET
HYL
ENE
BLU
E LO
FFLE
R
Met
hyle
ne B
lue
Loffl
er: A
) mou
th e
pith
el; B
)blo
od s
mea
r
num
ber o
f tes
ts10
0pr
oced
ure
time
~5 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15-2
5 °C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
A
lkal
ine
stai
n fo
r cel
l nuc
lei i
n sm
ears
from
diff
eren
t orig
in. P
atho
gens
(bac
te-
ria) e
xam
inat
ion.
Rea
gent
s:
Met
hyle
ne B
lue
solu
tion
acco
rdin
g Lo
ffler
Bac
teria
B
lue
Leuc
ocyt
es n
ucle
i B
lue
Epi
thel
ial c
ells
cyt
opla
smS
light
ly b
lue
Epi
thel
ial c
ells
nuc
lei
Blu
e
AB
39
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
NEW
MET
HYL
ENE
BLU
E
New
Met
hyle
ne B
lue
- blo
od s
mea
r
num
ber o
f tes
ts10
0pr
oced
ure
time
~20
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
B
lood
sm
ears
exa
min
atio
n fo
r ret
icul
ocyt
es. R
etic
uloc
ytes
are
juve
nile
red
cells
, whi
ch c
onta
in re
mna
nts
of b
asop
hilic
ribo
nucl
eopr
otei
ns. T
his
mat
eria
l rea
cts
with
the
dye
to fo
rm a
blu
ish
blac
k pr
ecip
itate
of g
ranu
les
or fi
lam
ents
.
Rea
gent
s:
New
Met
hyle
ne B
lue
wat
er, i
soto
nic
solu
tion,
tille
d w
ith a
ntic
oagu
lant
Ret
icul
ocyt
esG
reen
ish
to b
lue
with
dar
k bl
ue
ribon
ucle
opro
tein
gra
nule
s in
side
Ery
thro
cyte
sG
reen
ish
to b
lue
40
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
OIL
RED
O
Oil
red
O -
omen
tum
cry
osta
te s
ectio
n
num
ber o
f tes
ts10
0pr
oced
ure
time
~40
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Li
pid
stai
ning
in fr
ozen
sec
tions
.
Rea
gent
s:
A - P
ropy
lene
gly
col a
bsol
ute
C -
Pro
pyle
ne g
lyco
l 85%
wat
er s
olut
ion
Nuc
lei
Blu
e to
bla
ckLi
pids
RedB –
Sta
inin
g so
lutio
n O
il re
d O
D –
May
er’s
hae
mat
oxyl
in s
olut
ion
41
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
OR
CEI
N fo
r ela
stic
fibe
rs
num
ber o
f tes
ts10
0pr
oced
ure
time
~30
min
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Ela
stic
fibe
rs
Pur
plis
h re
d-br
own
Bac
kgro
und
Alm
ost c
olou
rless
To
dem
onst
rate
ela
stic
fibe
rs in
tiss
ue s
ectio
ns; r
ecom
men
ded
for v
ascu
lar
path
olog
y.
Rea
gent
s:
А –
Pot
assi
um p
erm
anga
nate
sol
utio
nС
– O
xalic
aci
d so
lutio
nЕ
– O
rcei
n so
lutio
n ac
cord
ing
Shi
kata
В –
Aci
d ac
tivat
ion
buffe
rD
– In
cuba
tion
med
iaF
– D
iffer
entia
tion
solu
tion
Orc
ein
for e
last
ic fi
bers
: A) b
lood
ves
sels
; B) k
idne
yA
B
42
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
PAPA
NIC
OLA
U
num
ber o
f tes
ts10
0pr
oced
ure
time
~25
min
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15 -
25 °
C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
S
tain
sol
utio
n fo
r cyt
o PA
P sm
ears
.
N.B
! EA
65 (E
mm
onya
Bio
tech
Ltd
.) is
an
alte
rnat
ive
for E
A50
sta
in, p
ro-
vidi
ng m
ore
inte
nsiv
e cy
topl
asm
sta
inin
g!
Rea
gent
s:
А –
Har
ris`s
hae
mat
oxyl
in s
olut
ion
C –
EA
50/6
5 po
lych
rom
ic s
tain
sol
utio
nВ
– O
rang
e G
-6 s
tain
sol
utio
n
Pap
anic
olau
for c
yto
PAP
smea
rs: A
) EA
50; B
)EA
65
Nuc
lei
Ora
nge-
brow
n to
blu
ish-
blac
kC
ytop
lasm
(ker
atin
ized
)O
rang
e-pi
nkC
ytop
lasm
(bas
ophi
lic)
Blu
e-gr
een
(EA
50) t
o tu
rquo
ise
(EA
65)
Cyt
opla
sm (m
etap
lasi
a ce
lls)
With
bot
h bl
ue a
nd p
ink
Cyt
opla
sm (e
osin
ophi
lic)
Pin
kE
ritro
cyte
sR
edM
icro
orga
nism
sB
lue-
viol
et to
blu
e-bl
ack
AB
43
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
PER
IOD
IC A
CID
SC
HIF
F (P
.A.S
.)
PAS
reac
tion
- gas
tric
tissu
e (s
tom
ach)
num
ber o
f tes
ts10
0pr
oced
ure
time
~35
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C,in
dar
k gl
ass
bottl
e, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
E
xam
inat
ion
of m
ucos
ubst
ance
s (m
ucop
olys
acch
arid
es),
lam
ina
basa
lae,
gl
ycog
en d
epos
its in
live
r etc
. in
tissu
e se
ctio
ns.
Rea
gent
s:
A –
Per
iodi
c ac
id s
olut
ion
C –
May
er’s
hae
mat
oxyl
in s
olut
ionn
Gly
coge
n, fu
ngi,
neut
ral m
ucos
ubst
anc-
es, a
nd s
ome
lam
ina
basa
lae
Mag
enta
Nuc
lei
Blu
e
B –
Sch
iff R
eage
nt
44
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
RA
PIH
EM
num
ber o
f tes
ts10
0pr
oced
ure
time
~ 40
sec
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Cel
lsC
lear
sha
ped
mor
phol
ogy
(nuc
leus
, cy
topl
asm
), du
e to
spe
cim
en o
rigin
! En-
ablin
g in
terp
reta
tion
of th
eir m
etha
bolit
ic
cond
ition
B
lood
sm
ears
, oth
er c
ytol
ogic
al s
peci
men
s an
d fin
e ne
edle
asp
iratio
ns e
xam
i-na
tion.
R
ecom
men
dabl
e fo
r fas
t blo
od s
mea
rs e
xam
inat
ion!
Rea
gent
s:
A –
Rap
ihem
fixa
tive
С –
Rap
ihem
sol
utio
n II
В –
Rap
ihem
sol
utio
n I
D –
Rap
ihem
was
hing
sol
utio
n -
conc
entra
te
Rap
ihem
: A
) Fin
e ne
edle
asp
iratio
n; B
) Blo
od s
mea
rA
B
45
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inst
ruct
ions
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se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
SUD
AN
III -
alc
ohol
sol
utio
n
Sud
an II
I alc
ohol
sol
utio
n - o
men
tum
cry
osta
te s
ectio
n
num
ber o
f tes
ts10
0pr
oced
ure
time
~35
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
S
tain
ing
of tr
igly
cerid
es in
froz
en ti
ssue
sec
tions
.
Rea
gent
s:
A –
50%
Eth
anol
C –
May
er’s
hae
mat
oxyl
in s
olut
ion
Nuc
lei
Blu
e to
bla
ckLi
pids
Ora
nge
B –
Sta
inin
g so
lutio
n S
udan
III (
alco
holic
so
lutio
n)
46
Full
inst
ruct
ions
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se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
SUD
AN
III -
Pro
pyle
ne G
lyco
l
Sud
an II
I Pro
pyle
ne G
lyco
l - o
men
tum
cry
osta
te s
ectio
n
num
ber o
f tes
ts10
0pr
oced
ure
time
~25
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
Tr
igly
cerid
es s
tain
ing
in fr
ozen
sec
tions
.
Rea
gent
s:
A –
Sta
inin
g so
lutio
n S
udan
III
C -
50%
Pro
pyle
ne g
lyco
l wat
er s
olut
ion
Nuc
lei
Blu
e to
bla
ckLi
pids
Ora
nge
B -
85%
Pro
pyle
ne g
lyco
l wat
er s
olut
ion
D –
May
er’s
hae
mat
oxyl
in s
olut
ion
47
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
TOLU
IDIN
E B
LUE
Tolu
idin
e B
lue
stai
ning
: A) s
kin;
B) u
teru
s
num
ber o
f tes
ts10
0pr
oced
ure
time
~6 m
in.
prod
uct v
alid
ity2
year
s (u
nder
reco
men
ded
cond
ition
s)st
orag
e co
nditi
ons
15-2
5 °C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
1.
To
dem
onst
rate
muc
opol
ysac
char
ides
and
oth
er m
etac
hrom
atic
sub
stan
ces
in ti
ssue
sec
tions
. 2. T
o de
mon
stra
te m
ast c
ells
(with
his
tam
ine,
hep
arin
met
achr
omat
ic
gran
ules
) in
tissu
e se
ctio
ns.
Rea
gent
s:
Tolu
idin
e bl
ue s
olut
ion
Tiss
ue s
truct
ure
Visi
ble
like
in s
tand
ard
Hae
mat
oxyl
in/
Eos
in h
isto
logy
sta
inin
g, b
ut in
blu
e sc
ale.
Mas
t cel
lsVi
olet
met
achr
omat
ic s
tain
ing
Pos
itive
con
trol
Ski
n
AB
48
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
VAN
GIE
SON
TR
ICH
RO
ME
Van
Gie
son
trich
rom
e st
aini
ng: A
) ski
n; B
) pla
cent
a
num
ber o
f tes
ts10
0pr
oced
ure
time
~35
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
S
tain
ing
of c
onne
ctiv
e tis
sue.
Rea
gent
s:
A –
Wei
gert’
s H
aem
atox
ylin
sol
utio
n B
C –
Van
Gie
son’
s pi
crof
uchs
in
Nuc
lei
Bla
ckC
olla
gen
fiber
s R
edM
uscl
e, c
orni
fied
epith
eliu
m, e
ryth
rocy
tes
Yello
w
B –
Wei
gert’
s H
aem
atox
ylin
sol
utio
n B
AB
49
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
WEI
GER
T fo
r ela
stic
fibe
rs
Wei
gert
for e
last
ic fi
bers
- ki
dney
num
ber o
f tes
ts10
0pr
oced
ure
time
~50
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
To
dem
onst
rate
ela
stic
fibe
rs in
tiss
ue s
ectio
ns; r
ecom
men
ded
for v
ascu
lar
path
olog
y.
Rea
gent
s:
А –
Per
iodi
c ac
id s
olut
ion
С –
Wei
gert’
s re
sorc
in fu
chsi
n so
utio
nЕ
– C
arm
alum
acc
ordi
ng to
May
er
solu
tion
Ela
stic
fibe
rs
Pur
plis
h re
d-br
own
Nuc
lei
RedВ -
Alc
ohol
ic re
agen
t for
incu
batio
n bo
xD
– A
cid
diffe
rent
iatio
n bu
ffer
50
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
WR
IGH
T’S
STA
IN fo
r cyt
olog
y
num
ber o
f tes
ts10
0pr
oced
ure
time
~2 m
inpr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
- 25
°C
, pro
tect
ed fr
om fi
re a
nd s
unlig
ht, t
ight
ly c
lose
d
Fi
ne n
eedl
e as
pira
tions
and
oth
er c
ytol
ogic
al s
peci
men
s ex
amin
atio
n
Rea
gent
s:
A –
Met
hano
l abs
olut
eС
– C
once
ntra
ted
Pho
spha
te b
uffe
r рН
6,8
В –
Wrg
ht’s
sta
inin
g so
lutio
n
Wrig
ht’s
Sta
in fo
r cyt
olog
y - fi
ne n
eedl
e as
pira
tion
Cel
lsC
lear
sha
ped
mor
phol
ogy
(nuc
leus
, cy
topl
asm
), du
e to
spe
cim
en o
rigin
! En-
ablin
g in
terp
reta
tion
of th
eir m
etha
bolit
ic
cond
ition
Ery
thoc
ytes
Pin
kish
-gre
en
51
Full
inst
ruct
ions
for u
se o
n w
ww
.em
mon
ya.c
omC
opyr
ight
Em
mon
ya B
iote
ch L
td. ©
CO
LD Z
IEH
L-N
EELS
EN
Zieh
l-Nee
lsen
sta
in -
sput
um
num
ber o
f tes
ts10
0pr
oced
ure
time
~45
min
.pr
oduc
t val
idity
2 ye
ars
(und
er re
com
ende
d co
nditi
ons)
stor
age
cond
ition
s15
-25
°C, p
rote
cted
from
fire
and
sun
light
, tig
htly
clo
sed
M
icro
scop
ic e
xam
inat
ion
of M
ycob
acte
ria.
Hig
her c
once
ntra
tion
of b
asic
fuch
sin
and
phen
ol in
Kin
youn
’s c
arbo
l fuc
hsin
sol
utio
n he
lps
pene
tratio
n of
Myc
obac
teria
’s c
ell w
all a
nd re
mov
es th
e ne
ed fo
r pre
-hea
ting
the
slid
es.
Rea
gent
s:
А –
Kin
youn
’s c
arbo
l fuc
hsin
sol
utio
nС
– M
ethy
lene
blu
e (w
ater
) sta
inin
g so
lu-
tion Tu
berc
le b
acill
i B
right
red
Bac
kgro
und
Ligh
t blu
e
В –
Aci
d al
coho
l sol
utio
n
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52
4. Reagents I
Method Reagent StabilityALCIAN BLUE pH 1- PAS A – Alcian blue staining solution рН 1 2
B – Periodic acid solution 2C – Schiff Reagent 2
ALCIAN BLUE pH 2.5– PAS A – Alcian blue staining solution рН 2,5 2B – Periodic acid solution 2C – Schiff Reagent 2
ALCIAN BLUE pH 2.5 A – Alcian blue staining solution рН 2,5 2B – Nuclear Fast Red staining solution 2
ALCIAN BLUE pH 1 A – Alcian blue staining solution рН 1 2B – Nuclear Fast Red staining solution 2
ALCIAN YELLOW – TOLUIDINE BLUE
А – Periodic acid solution 2В – Sodium bisulfite solution 2С – Alcian yellow staining solution 2D – Toluidine blue staining solution 2Е – Sodium hydroxide solution 2
AZAN TRICHROMЕ А – Azocarmine solution 2В – Aniline solution 2C – Differentiation solution 2
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53
D – Phosphomolybdic acid solution 2E – Staining solution 2
BRILLIANT CRESYL BLUE Brilliant Cresyl Blue physiological solution 2BENNHOLD’S CONGO RED A – Congo red staining solution according
Benhold2
B – Sodium hydroxide solution 2C – Mayer’s haematoxylin 2
HIGHMAN’ S CONGO RED A- Congo red staining solution according Highman
2
B- Potassium hydroxide solution 2CYTOMAX А – Harris`s haematoxylin solution 2
B – 2-Propanol Absolute 2C – Modified polychromic EA65 solution 2D – 80% 2-Propanol 2
EOSIN – ACQUEOUS SOLUTION 1%
Eosin Y 1% water solution 2
EOSIN – ACQUEOUS SOLUTION 5%
Eosin Y 5% water solution 2
EOSIN – ALCOHOLIC SOLUTION 0.5%
Eosin Y 0.5% alcoholic solution 2
EOSIN-PHLOXINE ALCOHOLIC SOLUTION
А – Mayer’s haematoxylin 2В – Scott’ s solution (water) 2С – Eosin-Phloxine staining solution 2
GIEMSA STAIN Giemsa concentrate solution 2GILL’S I HAEMATOXYLIN Gill’s 1 Haematoxylin solution 2
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54
GILL’S II HAEMATOXYLIN Gill’s 2 Haematoxylin solution 2GILL’S III HAEMATOXYLIN Gill’s 3 Haematoxylin solution 2GOMORI SILVER IMPREGNATION
А – Potassium permanganate solution 2В – Acid activating buffer 2С – Oxalic acid solution 2D – Ferric ammonium sulfate solution (alum) 2E – Ammoniacal silver solution 1F – Neutral formalin solution 2G – Sodium hyposulphite solution 2
GRAM STAIN A – Crystal Violet solution 2B – Lugol’s solution for Gram 2C – Decolorising solution 2D – Safranin O alcoholic solution 2
GROCOTT’S А – Chromic acid solution 2В – Sodium hydrogensulfite solution 2С – Methenamine solution 2D – Silver nitrate solution 2E – Borax water solution 2F – Gold chloride water solution 2G – Sodium thiosulfate solution 2H – Light Green solution 2
HAEMATOCOLOR A – Methanol absolute 2B – Haematocolor solution I 2
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55
C – Haematocolor solution II 2D - Haematocolor Phosphate buffer 2
HARRIS’S HAEMATOXYLIN Harris’s haematoxylin solution 2LEISHMAN STAIN A – Leishman Staining solution 2
B – Buffered water 2LEISHMAN- WRIGHT STAIN A – Leishman-Wright staining solution 2
B – Buffered water 2MALLORY TRICHROMЕ А – Acid fuchsin staining solution 2
B – Phosphomolybdic acid solution 2C – Mallory’s staining solution 2
MASSON TRICHROME GOLDNER
A- Weigert’s Haematoxylin solution A 2B- Weigert’s Haematoxylin solution B 2C- Acid fuchsin staining solution 2D- Phosphotungstic acid solution 2E- Light green staining solution 2
MASSON TRICHROME STANDARD
A- Weigert’s Haematoxylin solution A 2B- Weigert’s Haematoxylin solution B 2C- Acid fuchsin staining solution 2D- Phosphomolybdic acid solution 2E- Phosphotungstic acid solution 2F- Masson’s aniline blue solution 2G- Acetic acid solution 2
MAY GRÜNWALD - GIEMSA A – Methanol absolute 2
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56
B – May-Grunwald staining solution 2C – Giemsa staining solution 2D – Sorenson (Sorensen) buffer pH7 2
MAYER’S HAEMATOXYLIN Мayer’s Haematoxylin solution 2MAY - GRUNWALD STAIN May - Grunwald Stain solution 2METHENAMINE SILVER IMPREGNATION – P.A.S.M
А – Periodic acid solution 2В – Silver nitrate solution 1C – Hexamethylene tetraamine solution 1D – Sodium tetraborate solution 2E – Gold chloride solution 1F – Fixative 2
METHYLENE BLUE LӦFFLER Methylene Blue solution according LofflerNEW METHYLENE BLUE New Methylene Blue water, isotonic solution,
tilled with anticoagulantOIL RED O A – Propylene glycol absolute
B – Staining solution Oil red OC – Propylene glycol 85% water solutionD – Mayer’s haematoxylin solution
ORCEIN А – Potassium permanganate solutionВ – Acid activation bufferС – Oxalic acid solutionD – Incubation mediaЕ – Orcein solution according ShikataF – Differentiation solution
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57
PAPANICOLAU А – Harris`s haematoxylin solutionВ – Orange G-6 stain solutionC – EA50 polychromic stain solutionC – EA65 polychromic stain solution
PERIODOC ACID SCHIFF (PAS)
A – Periodic acid solutionB – Schiff ReagentC – Mayer’s haematoxylin solution
RAPIHEM A – Rapihem fixativeВ – Rapihem solution IС – Rapihem solution IID – Rapihem washing solution - concentrate
SUDAN III PROPYLENE GLYCOL
A – Staining solution Sudan IIIB – 85% Propylene glycol water solutionC - 50% Propylene glycol water solutionD – Mayer’s haematoxylin solution
SUDAN III ALCOHOL SOLUTION
A – 50% EthanolB – Staining solution Sudan III (alcoholic solution)C – Mayer’s haematoxylin solution
TOLUIDINE BLUE STAIN Toluidine blue solutionVAN GIESON TRICHROME STAIN
A – Weigert’s Haematoxylin solution AB – Weigert’s Haematoxylin solution BC – Van Gieson’s picrofuchsin.
WEIGERT for elastic fibers А – Periodic acid solution
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58
В - Alcoholic reagent for incubation boxС – Weigert's resorcin fuchsinD – Acid differentiation bufferЕ – Carmalum according to Mayer
WEIGERT’S HAEMATOXYLIN A – Weigert’s Haematoxylin solution AB – Weigert’s Haematoxylin solution B
WRIGHT’S STAIN for cytology A – Methanol absoluteВ – Wrght’s staining solutionС – Concentrated Phosphate buffer рН 6,8
COLD ZIEHL-NEELSEN A - Kinyoun’s carbol-fuchsin solutionB - Acetic alcohol solutionC - Methylene Blue solution
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59
4. Reagents II
Products Code Size DescriptionAcid ethanol Shikata 155802125E 125ml Differentiating agent
155802250E 250ml155802500E 500ml
Acid ethanol Weigert 153812125C 125ml Differentiating agent153812250C 250ml153812500C 500ml153812100C 1l
Hidrocloric Acid (1M) 1090570125 125ml Differenting agent1090570250 250ml1090570500 500ml1090570100 1l
Kovach’s indole reagent 119002125 125ml For identification of indole119002250 250ml119002500 500ml119002100 1l
Orcein Acetic 1558011125 125ml For chromosomal staining
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60
Oxalic Acid 1099650125 125ml Decolorant1099650250 250ml1099650500 500ml1099650100 1l
Phosphate buffer pH 6.8 113273125 125ml113273250 250ml113273500 500ml113273100 1l
Phosphate buffer pH 7.2 111316125D 125ml111316250D 250ml111316500D 500ml111316100D 1l
Picric Acid aqueous sol. 1.2% 110901125 125ml For fixatives mixtures110901250 250ml110901500 500ml110901100 1l
Picric Acid alcoholic sol. 110902125 125ml For erythrocytes in trichrome staining110902250 250ml110902500 500ml110902100 1l
Picrofuchsin 1159270125 125ml For cytoplasm, muscle and collagen
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61
Potassium Methabisulfite 0.5% 105057125 125ml Differentiating agent105057250 250ml105057500 500ml105057100 1l
Sodium Thiosulphate 5% 109950125 125ml Decolorant after treatment with lugol solution109950250 250ml
109950500 500ml109950100 1l
Sulphuric Acid 0.5% 109981125 125ml For oxidation with permanganate109981250 250ml109981500 500ml
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62
Product Supplementary Code DescriptionDEK – HL DEK-HL decalcifying solution 111300910 For fibrillar and
lamellar bone structureAcid solution for DEK-HL decalcifying solution 111300910.1
Ammonium solution for DEK-HL decalcifying solution 111300910.2DEK – HN DEK-HN decalcifying solution 111300510 For bone tissue
Sodium solution for DEK-HN decalcifying solution 111300510.1DEK – MF DEK-MF decalcifying solution 111300410 Universal
6. Decalcifying solutions
Products Size Code DescriptionBouin 500ml 11400500 Picric acid/formalin
fixative1000ml 1140010002500ml 114002500
Saccomano 25ml 199001025 Fixative for cytology speciments1000ml 199001000
7. Fixatives for histology
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63
Products Size Code DescriptionFormaline 38-40% 1L 10561000
5L 10561000Formaline 30% w/v 5L 10561000
1L 10561000Formalin 10% solution pH7.2-7.4 buffered
1L 105710005L 105755000
8. Formalins
Products Size Code DescriptionBio Clear 1L 10271000
5L 10275000Prohyol 95 1L 10075000
5L 10071000Prohyol absolute 1L 10775000
5L 10771000
9. Solvents
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64
Products Size Code DescriptionParaffin wax histology 56-58°C 1kg 313110 Lepka 100ml 059801Eurokit 100ml 0405003Canada balsam 100ml 157160598
10. Mounting and embed media
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65
• Acute toxicity (oral, dermal, inhalation), category 4• Skin irritation, categories 2, 3• Eye irritation, category 2A• Skin sensitization, category 1• Specific target organ toxicity following single exposure, category 3 o Respiratory tract irritation o Narcotic effects
Acute toxicity (oral, dermal, inhalation), categories 1, 2, 3
• Flammable gases, category 1 • Flammable aerosols, categories 1, 2 • Flammable liquids, categories 1, 2, 3 • Flammable solids, categories 1, 2
• Skin corrosion, categories 1A, 1B, 1C• Serious eye damage, category 1
11. Legend