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EDWIN
2015/09/18
DIY ATOMIC FORCE MICROSCOPE(AFM)
&
LIFELONG LEARNING
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
Exploring the micro world by an optical microscope
Seeing is believing?
Diffraction limit of light
100~20k USD We can not “see” the nanoworld
Scanning Electron Microscope (SEM)
http://www.jeolusa.com/PRODUCTS/ScanningElectronMi
croscopes(SEM)/SEM-FIB/JIB-
4600FMultiBeam/tabid/543/Default.aspx
500k~2M USD
Image only conductive samples
Electron gun
DetectorMagnetic lens
Electron
“touching” the nanofeatures ….
Image both conductive and non-conductive samples in
air, liquid and vacuum environments
50k~500k USD https://en.wikipedia.org/wiki/Atomic_force_microscopy
Atomic force microscope (AFM)
Exploring the Nano world By a very sharp tip….
Year 2013, Francois was looking for
an affordable AFM that can be easily built
10
Nano optics
replaced by DVD OPU Head Nano scanner
replaced by piezo buzzers
Metal parts replaced
by structures made of PCBs Optical table replaced
by handheld anti-vibration table
4 techniques make the AFM affordable
Inspired by Sebastian Butefisch
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
13
The DIY AFM kit
http://www.stromlinet-nano.com/
http://www.stromlinet-nano.com/
Primary / junior high school children
assembling / using the DIY AFM
Tearing the parts AFM base Buzzer scanner
AFM head Within 2 hours Measuring nanostructures
Lego2Nano Lego2Nano Lego2Nano
15
Move the AFM
from Clean Room to Class Room
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
Microscope for children
in the past 100 years: 1,000x
Onion cells
Area: 200 x 200 mm
Optical microscope for children
Microscope for children
Now: Magnification 100,000,000 x
DNA origami on Mica substrate
Area: 5 x 5 mm, AC mode
AFM for children
DNA origami
Sample prepared by Bryan Wei @Tsinghua University http://www.stromlinet-nano.com/
AFM topographic image
Area: 5x5 mm
Optical image
Area: 600x600 mm
Measuring surface of an eraser
AFM topographic image
Area: 15x15 mm
Optical image
Area: 600x600 mm
Measuring fresh leaf surface
AFM topographic image
Area: 8x8 mm
Optical image
Area:900x900 mm
Measuring data tracks on a
DVD disk
22
AFM Measurement
3D printer
Nano topography data 3D “Solid data”
New idea!
Science project demonstration
AFM data & 3D printing
DIY AFM Laser microscope mode
Imaging astrocytes
1000X OM phase contract image
with Oil lens (Contrast: 0.143) Laser microscope mode (Contrast: 0.224)
10 mm 10 mm
Wesley W. HSIAO,Hsien-Shun LIAO,Hsing-Hung LIN, Yueh-Lun LEE, Chia-Kwung FAN, Chien-Wei LIAO, Po-Yen LIN, En-Te HWU,
and Chia-Seng CHANG , Analytical Sciences, 29(9), 885 (2013)
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
LEGO2NANO Summer school
14~28th August 2015
Reinvent the DIY AFM
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
Air pollution (PM 2.5) particles found
on the DVD data tracks in Beijing
Diameter ~2,300 nm (PM2.5)
Outline
• Different microscopes for different scales
• Hands-on learning: DIY AFM
• Microscope for children
• Lifelong learning: LEGO2NANO summer school
• Air pollution (PM 2.5) particles detection
• Online science participation: Crowdcrafting
Crowdcrafting and maybe EpiCollect
BY Daniel Lopez Martinez @ LEGO2NANO 2015 SUMMER SCHOOL
Thank you !!