331 ENDOSULFAN (032) EXPLANATION Endosulfan has been reviewed many times from 1967 to 1989, at which time a full re-evaluation was carried out. As a result of information that proposed MRLs for endosulfan on several brassica crops did not reflect GAP in the USA, the 1992 CCPR placed the recommendations in step 7B, pending review by the JMPR. Additional GAP and residue data have now become available and are reviewed below. USE PATTERN Endosulfan is widely used as an insecticide on fruits, vegetables, cereals and oilseed. GAP information from 21 countries was provided by the main manufacturer, Hoechst AG; this is summarised in Table 1. Table 1. Endosulfan - registered use rates and patterns Country Crop EC/WP /DP Conc Applic. (kg ai/ha) No.of app. PHI (days) Comments Argentina Vegetables EC 35% 0.60 1 7 Post-Flowering Soya bean EC 35% 0.45 1 14 Post-Flowering Sunflower EC 35% 0.50 1 14 Flowering Australia Pineapple EC 35% [0.56-0.7 g/l] 1 - Post-harvest dip Belgium Fruits EC 35% 0.35-1.75 - 28 4 wks pre-harvest Berries EC 35% - 42 Strawberry & EC 35% 0.525 - 28 Before flowering small fruits Mushrooms EC 35% 14 - 21 3 wks pre-harvest Potato EC 35% 0.175-0.525 - 28 4 wks pre-harvest Rape EC 35% 0.6-0.7 - 28 Brazil Soya bean EC 35% 0.26 1 30 Cotton EC 35% 0.525 1 30 Coffee EC 35% 0.525 1 70 Canada Apple & Pear WP 50% 1.6-3.4 Sev. 15 Apricot, Cherry, Peach & Plum EC/WP 40/50% 0.48-0.76 2-3 15 Avoid spray on fruit Grapes WP 50% 1.25-1.5 2 30 Strawberry EC 40% 1.0-5.4 1-2 7 Pre-bud & after harvest WP 50% 1.0-16 1-2 7 Pre-bud & after harvest Broccoli, Brussels sprouts, Cabbage & Cauliflower EC/WP 40/50% 0.55-0.88 Sev. 7 7 day intervals DP 2% 0.8-1.0 Sev. 7 7-10 day intervals Cucumber, Melon, Pumpkin & Squash EC/WP 40/50% 0.5-0.6 1-4 2 7 day intervals DP 2% 0.8-1.0 4 5 7-10 day intervals Egg plant, Peppers & Tomato EC 40% 0.5-0.6 Sev. 2 7 day intervals DP 2% 1.0-1.1 Sev. 5 7-10 day intervals Sweet Corn EC/WP 40/50% 1.0-1.7 1-2 50 Lettuce EC/WP 40/50% 0.8-0.88 1-3 14
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331
ENDOSULFAN (032)
EXPLANATION
Endosulfan has been reviewed many times from 1967 to 1989, at which time a fullre-evaluation was carried out. As a result of information that proposed MRLs for endosulfanon several brassica crops did not reflect GAP in the USA, the 1992 CCPR placed therecommendations in step 7B, pending review by the JMPR. Additional GAP and residuedata have now become available and are reviewed below.
USE PATTERN
Endosulfan is widely used as an insecticide on fruits, vegetables, cereals and oilseed. GAPinformation from 21 countries was provided by the main manufacturer, Hoechst AG; this issummarised in Table 1.
Table 1. Endosulfan - registered use rates and patterns
Country Crop EC/WP/DP
Conc Applic.(kg ai/ha)
No.ofapp.
PHI(days) Comments
Argentina Vegetables EC 35% 0.60 1 7 Post-Flowering
Soya bean EC 35% 0.45 1 14 Post-Flowering
Sunflower EC 35% 0.50 1 14 Flowering
Australia Pineapple EC 35% [0.56-0.7 g/l] 1 - Post-harvest dip
Clementine. In one trial in Spain in 1992, residues were below0.1 mg/kg 14 days after application.
Lemon. Residues in lemons were up to 0.16 mg/kg at 7 days and0.12 mg/kg at 14 days after treatment.
Oranges. Residues from three trials in Spain at GAP rates didnot exceed 0.35 mg/kg at 15 days PHI. In five other trials at aslightly excessive application rate, one residue of 0.54 mg/kgwas observed at 14 days; all other determinations were below 0.4mg/kg.
Pome fruits
Apple. From five trials on apples in Germany, a maximum residueof 0.63 mg/kg was found at 21 days PHI. The current CXL for pomefruits is 1 mg/kg.
Stone fruits
Cherry, Sour. In four trials on Morello cherries in Germany in1983 the maximum residue found at 21 days PHI was 0.03 mg/kg,well within the current CXL of 1 mg/kg.
Peach. Use of a 2.8% dusting powder in five trials on peaches inGermany resulted in residues up to 0.5 mg/kg at 14 or 21 days.Two trials in Spain using 35% EC gave a maximum residue of 0.73mg/kg at 15 days. None of the trials was within current GAP inthese countries.
Plums. In seven trials in Germany, from 1983 to 1989, a maximumresidue of 0.38 mg/kg was found at 21 days PHI.
Grapes. Data were provided on 15 trials on grapes in Germanyfrom 1974 to 1987. At a PHI of 60 to 62 days, residues were inthe range 0.49-0.6 mg/kg.
Strawberry. Three trials on strawberries in Spain in 1985 showedresidues up to 4 mg/kg at 3 days, 2.5 mg/kg at 4 days and 0.84mg/kg at 13 days after application. This treatment is not GAP inSpain.
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Pineapple. Endosulfan is used in Australia for quarantinepurposes as a post-harvest dip. Residues up to 2 mg/kg can beexpected from this procedure which would be within the existingCXL of 2 mg/kg for "Fruits".
Table 2. Residues of endosulfan in fruit from supervised trials.
Crop/Country/Year
EC/WP/DP/%
Appl. No. oftrials
Residues (mg/kg) at intervals(days) after last appl.
The only residue data from the USA that were presented were fromtwo trials on Brussels sprouts carried out in 1964. At 14 daysPHI, residues reached 1.2 mg/kg. From one trial on Brusselssprouts in the UK in 1976, residues reached 0.1 mg/kg at 14 daysand 0.06 mg/kg at 21 days PHI. One trial on broccoli at the GAPrate in Portugal gave 0.44 mg/kg at 28 days PHI, just inconcordance with the existing CXL of 0.5 mg/kg.
Residues from six trials on head cabbage in Germany andPortugal showed maximum residues of 0.73 mg/kg at 27 days; thecurrent CXL is 1 mg/kg. Data from 26 trials on Savoy cabbage inGermany showed no residues above 0.42 mg/kg at 14 days; thecurrent CXL is 2 mg/kg. On cauliflower, four trials in Germanyshowed residues below 0.2 mg/kg at 10 days and 0.1 mg/kg at 14days PHI.
Information from Canada on Chinese broccoli and mustardcabbage showed residues up to 1.3 mg/kg at 7 days.
Table 3. Residues of endosulfan in brassica vegetables fromsupervised trials.
Crop/Country/Year
EC/WP/DP/%
Appl. No.oftrials
Residues (mg/kg) at intervals (days) after last application
Ref.
kg/ha No.
Broccoli
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Crop/Country/Year
EC/WP/DP/%
Appl. No.oftrials
Residues (mg/kg) at intervals (days) after last application
Cucurbits. At a PHI of 7 days, five trials on cucumbers inCanada showed a maximum residue of 0.17 mg/kg from treatments atslightly above the current GAP rate. Greenhouse cucumberstreated in Spain in 1992 showed a residue of about 0.1 mg/kg at7 days PHI.
Greenhouse-grown melons in Spain showed residues up to 0.5mg/kg after 7 days. Residues from trials on squash in Canada andSpain were up to 0.23 mg/kg at 7 days PHI.
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Peppers. Two trials on greenhouse peppers in Spain showedresidues up to about 0.5 mg/kg at 7 days.
Tomato. Data were available from 28 field trials in Germany andone in Canada. At a PHI of 7 days residues were generally below0.2 mg/kg, although in one trial a level of 1 mg/kg was found.
Tomatoes treated in greenhouses in Spain showed residues upto 1 mg/kg at 7 and 15 days PHI, but at rates of applicationthat were above the quoted GAP.
Lettuce. Two trials in Canada gave residues up to 0.15 mg/kg at14 days, well within the current CXL of 1 mg/kg.
Common bean. From 20 trials on common beans in Canada, Germanyand Spain, the maximum residue observed at a PHI of 14 days was0.59 mg/kg. At 3 days PHI, up to 0.97 mg/kg was found.
Broad beans. Twelve trials on broad beans in Germany gaveresidues up to 1.9 mg/kg at 7 days and 0.04 mg/kg at 21 days. Nodata were provided at the GAP PHI of 14 days.
Soya beans. Data were supplied from Australia, 2 trials, andBrazil, 42 trials. At the recommended PHI of 30 days, themaximum residue was 0.6 mg/kg; all of the other findings werebelow this at PHIs ranging from 13 to 101 days.
Potato. Residues from seven trials in Germany were all below0.015 mg/kg at PHIs from 0 to 28 days.
Celery. Two trials in Canada showed residues well below theexisting CXL of 2 mg/kg at 0 to 35 days after treatment.
Table 4. Residues of endosulfan in other vegetables fromsupervised trials.
CropCountry/Year
EC/WP/DP/%
Appl. Residues (mg/kg) at intervals (days) afterlast application
Cotton seed. From eight trials in Spain the maximum residue at aPHI of 15 days was 0.25 mg/kg.
Rape seed. Results from 24 trials on rape seed in Germany showedresidues up to 0.30 mg/kg at 56 days PHI when GAP applicationrates were used. A level of 0.57 mg/kg was found when over twicethe recommended rate was applied.
Sunflower seed. In 5 trials in the USA in 1965 residues up to0.6 mg/kg were found 63 to 69 days after treatment. ULVapplication in the Sudan in 1985 gave residues of 0.008-0.036mg/kg after 109 days.
Table 6. Residues of endosulfan in oilseed from supervisedtrials.
Crop/Country/Year
EC/WP/DP/%
Appl. No. oftrials
Residues (mg/kg) at intervals (days) after last application
Ref.
kg/ha No.
Cotton seed
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Crop/Country/Year
EC/WP/DP/%
Appl. No. oftrials
Residues (mg/kg) at intervals (days) after last application
Cacao. Trials were carried out in Brazil (4) and the IvoryCoast (3). All residues were below 0.02 mg/kg at 28 to 45 daysafter treatment.
Coffee. Trials on coffee were carried out in Brazil (6),Cameroon (60) and Guatemala (4). Results were all expressedseparately as on the surface and in the interior of the greenbeans. All residues were below 0.05 mg/kg at PHIs ranging from30 to 205 days. Table 7. Residues of endosulfan in seeds for beverages fromsupervised trials.
Apples. In two trials on apples in Germany in 1989, fruit withresidue levels of 0.055 and 0.11 mg/kg gave apple juicecontaining less than 0.006 mg/kg, while the respective pomacesheld 0.08 and 0.18 mg/kg. (Hoechst, A47389-90).
Grapes. Grapes treated in Germany in 1984 which containedresidues of endosulfan at levels of 0.49 and 0.55 mg/kg wereused to make wines. These contained less than 0.006 mg/kg, whilethe musts yielded 0.03 and 0.04 mg/kg respectively (Hoechst,A30914-5).
Common beans. In Canada, in 1988, a study was made of the effectof various culinary procedures on residues in beans. Themeasures used included trimming, water rinsing, boiling,blanching, microwave cooking and freezing. The results obtainedare summarized below and show that boiling and trimming off theends are the major causes of residue loss (Canada, 1993).
Wheat. Bread was made from wheat containing residues ofendosulfan at 0.19 mg/kg. Residues in wholemeal bread were 0.13mg/kg, in refined meal bread 0.049 mg/kg and in the bran 0.28mg/kg.
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NATIONAL MAXIMUM RESIDUE LIMITS
The Meeting received information on the following national MRLs.
Commodity MRL, mg/kg
Austr1
Belg2 Can3 Den4 Ger5 Spain
Alfalfa 0.1
Apple 2
Asparagus 1
Barley 0.1
Berries, etc. 1 2
Blackberry 1
Brassicae 1
Broccoli 2
Brussels sprouts 2
Cabbages 2 1
Carrot 0.2
Cauliflower 1 1
Celery 1
Cereals 0.2
Citrus fruits 1
Clover 0.1
Common bean 1 1
Cotton 1
Cotton seed oil(crude)
0.5
Cucumbers 1
Cucurbits 1
Currants, Black 2
Currants, Red &White
1
Dairy products 0.1
Egg plant 1 1
Eggs 0.05
Fat of meat ofcattle
0.2
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Commodity MRL, mg/kg
Austr1
Belg2 Can3 Den4 Ger5 Spain
Fat of meat ofgoats
0.2
Fat of meat ofsheep
0.2
Fruit 2
Goat milk (fatbasis)
0.5
Grapes 1 1
Hazlenut 1
Hops 10
Lettuce, Head 2
Lupins 1
Meat 0.1
Melon 1
Milk (fat basis) 0.5
Milk products (fatbasis)
0.5
Mung beans 1
Mushrooms 0.05
Navy beans 1
Nuts 0.2
Oats 0.1
Oil seeds 1
Olive 1
Onion 0.2
Other vegetables 2
Parsley 0.1
Peanuts 1
Pear 2
Peas 1
Pepper 1 1
Pome fruit 2 1
Potato 0.1 0.1 0.1
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Commodity MRL, mg/kg
Austr1
Belg2 Can3 Den4 Ger5 Spain
Poultry meat 0.2
Pumpkin 1
Rape 0.05 0.5
Raspberry 1
Rice (in husk) 0.1
Rutabaga 0.1
Rye 0.1
Soya bean 1
Spinach 2
Squash 1
Stone fruit 2 2 1 1
Strawberry 1
Sugar beet 0.1
Sunflower 0.1
Sweet corn 0.1
Sweet potato 0.2
Tea (drymanufactured)
30
Tomato 1 1 1
Turnip 0.1
Water 0.04
Watercress 0.1
Wheat 0.1
1 Australia 2 Belgium 3 Canada 4 Denmark 5 Germany
APPRAISAL
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Endosulfan has been reviewed by the JMPR eight times since 1967, including a major re-evaluation in 1989. At the 24th (1992)Session of the CCPR it was pointed out (ALINORM 93/24, paras81-86) that the MRLs for head cabbages, Savoy cabbage andcauliflower did not reflect the residues expected from GAP inthe USA. MRLs for broccoli, Brussels sprouts, head cabbage andSavoy cabbage were therefore held at Step 7B pending review bythe present Meeting. The proposed deletion of the general MRLsfor "Fruit" and "Vegetables, except as otherwise listed", asrecommended by the 1989 JMPR, was also delayed until after thisreview.
Information on the current GAP of 21 countries was madeavailable to the Meeting by the manufacturer, including fulldetails from the USA. A large quantity of residue data that hadnot previously been submitted for review was also provided.
Residue data from supervised trials on many fruits,vegetables, cereals, oilseed and beverage seeds which had notbeen reviewed previously were also provided and are recorded inthe monograph on this compound. The Meeting confirmed that thedata emphasised the desirability of withdrawing the currentgeneral MRLs for fruit and vegetables and replacing them withMRLS for individual commodities, usually at a lower level. Itwas also possible to make recommendations for MRLs on someadditional crops.
The residue data on oranges were adequate to allow an MRLof 0.5 mg/kg to be recommended but the data on clementines andlemons were only in summary form and thus not sufficient toextend the MRL to the citrus fruit group.
Residues from trials on apples, cherries and plums werewithin the current MRLs of 1 mg/kg. Data for peach residuessupported a similar MRL of 1 mg/kg. Residue data on grapes alsoallowed an MRL of 1 mg/kg to be recommended but the strawberrydata were only summaries and were thus inadequate.
A dip treatment of pineapples with endosulfan is requiredin Australia for export quarantine purposes. The resultantresidues are up to 2 mg/kg, within the CXL for "Fruits", and soan MRL of 2 mg/kg was recommended to cover this post-harvestuse.
Unfortunately, the only residue data on brassica cropstreated in the USA concerned two trials on Brussels sprouts thatwere carried out in 1964 and had been reported previously; at a14-day PHI a maximum residue of 1.2 mg/kg was observed. In onetrial on Brussels sprouts in the UK in 1976, 0.1 mg/kg wasfound after 14 days and 0.06 mg/kg after 21 days but the datawere not adequate to support an MRL recommendation. Data fromother countries under their GAP conditions were available forbroccoli, head cabbage, Savoy cabbage and cauliflower; these results were consistent with the existing draft MRLs of0.5, 1, 2 and 0.5 mg/kg, respectively.
For some other vegetables, currently covered by the CXL for"Vegetables, except as otherwise listed", the data presented
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were sufficient to allow recommendations to be made for broadbean, cucumber, melons except watermelon, summer squash andtomato, all at 0.5 mg/kg, and for soya bean at 1 mg/kg. Datafor sweet peppers were inadequate.
Residues on celery (2 mg/kg), common bean (0.5 mg/kg), headlettuce (1 mg/kg) and potato (0.2 mg/kg) were within therespective CXLs.
For cereals, residue data were presented for maize andwheat, allowing recommendations of 0.1 mg/kg and 0.2 mg/kg,respectively, to be made.
Trials on some oilseeds gave sufficient residue data forMRLs to be recommended for cotton seed (1 mg/kg), rape seed (0.5mg/kg) and sunflower seed (1 mg/kg).
New residue data were also available which allowedrecommendations to be made for MRLs on cacao beans and coffeebeans, both at 0.1 mg/kg.
Processing data were available for apples (juice andpomace), grapes (wine and must) and common beans (washing andcooking).
RECOMMENDATIONS
On the basis of the data on residues resulting from supervisedtrials the Meeting concluded that the residue levels listedbelow are suitable for establishing maximum residue limits.
Definition of the residue: sum of alpha- and beta-endosulfan andendosulfan sulphate (fat-soluble).
Commodity Recommended MRL(mg/kg)
PHI onwhichbased,days
CCN Name New Previous
VP 0522 Broad bean 0.5 21 21
VB 0400 Broccoli 0.5 0.5 28
VB 0403 Cabbage, Savoy 2 2 14
VB 0041 Cabbages, Head 1 1 27
SB 0715 Cacao beans 0.1 --- 28
VB 0404 Cauliflower 0.5 0.5 7
VS 0624 Celery 2 2 14
FS 0013 Cherries 1 1 21
SB 0716 Coffee beans 0.1 --- 30
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Commodity Recommended MRL(mg/kg)
PHI onwhichbased,days
CCN Name New Previous
VP 0526 Common bean 0.5 0.5 14
SO 0691 Cotton seed 1 1 15
VC 0424 Cucumber 0.5 21 7
AO20001
Fruits W 2 --
FB 0269 Grapes 1 22 60
VL 0482 Lettuce, Head 1 1 14
GC 0645 Maize 0.1 --- 54
VC 0046 Melons, except Watermelon 0.5 21 7
FC 0004 Oranges, sweet, sour 0.5 22 14
FS 0247 Peach 1 22 15
FI 0353 Pineapple 2 Po 22 --
FS 0014 Plums (including Prunes) 1 1 21
FP 0009 Pome fruits 1 1 21
VR 0589 Potato 0.2 0.2 28
SO 0495 Rape seed 0.5 --- 56
VD 0541 Soya bean 1 21 30
VC 0431 Squash, Summer 0.5 21 7
SO 0702 Sunflower seed 1 --- 63
VO 0448 Tomato 0.5 21 7
AO10002
Vegetables, except asotherwise listed
W 2 --
GC 0654 Wheat 0.2 --- 14
1 Previously "Vegetables, except as otherwise listed", 2mg/kg.
2 Previously "Fruits", 2 mg/kg.
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FURTHER WORK OR INFORMATION
Required (by 1996)
Residue data from supervised trials on brassica crops carriedout in the USA under their current GAP.
REFERENCES
(all references are unpublished)
Australia, 1993. Data on GAP and residues supplied by Australiafor JMPR.
Canada, 1993. Data on GAP and residues supplied by Canada forJMPR.
Hoechst AG, 1993. A large number of residue reports in theA..... series, asindicated in the Tables or text as appropriate.
Portugal, 1993. Data on GAP and residues supplied by Portugalfor JMPR.
Spain, 1993. Data on GAP and residues supplied by Spain forJMPR.
