Enhance the Capacity for Species Identification and Genetic Analysis on Marine Organisms in

the Coral Reef Ecosystems in the Western Pacific (DRMREEF) - PHILIPPINES

PROJECT UPDATE

Ephrime B. Metillo Project Leader-PHILIPPINES

PHILIPPINES DNA BARCODING:

2014 review: very few (barcoded marine

species

Biodiversity = societal needs

Phase 1: 200 Species submitted, 96 species with DNA barcodes

DEPOSITED in Mindanao State University - Iligan Institute of Technology

Natural History Museum

Phase 2: 211 Species submitted without DNA barcodes

1 2 2

2

2

2

Iligan City

Iligan Bay

Bohol Sea

PRISM: Premier Research Institute of Science and Mathematics

Marine Biodiversity Research Laboratory

PCR Machine (MJ Reasearch PTC200 Model

Vortex Centrifuge

Pipettors 1 whole set plus stand

PCR Products microcentrifuge set (with

angle rotor PCR tubes) ...

Refrigerated centrifuge

Reagents: QIAGEN DNEasy Tissue Extraction Kit

(supplies)

DNA bands UV transilluminator (Accuris E3000)

Centrifuge tubes and tips (supplies)

MSU-IIT IOC-WESTPAC

DRMREEF2

Sampling Funds

Other lab equipment

Mupid gel electrophoresis machine

Fume hood

MARINE BIODIVERSITY: DNA BARCORDING LABORATORY

DRMREEF 2 Fish 45 82 211

DRMREEF 1 Fish 48 162 192

Family Species Specimens Group

DRMREEF 1 Lobster 1 1 1

DRMREEF 1 Corals 2 7 7

LUTJANIDAE SCARIDAE

MULLIIDAE SERRANIDAE

HOPES:

- Proposal submitted to Philippine

government approved

- Smooth running of the DNA barcoding lab

- Biodiversity research appreciated by the

public and politician

- International funding support for reef

recruitment studies (techno-demo site)

THANKS TO:

• UNESCO IOC-WESTPAC

• MSU-IIT Administration

• People

FOR SUPPORT AND COOPERATION