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Enzyme-Linked Immunosorbent Assay(Direct ELISA)
Figure 18.14a
Enzyme-Linked Immunosorbent Assay (Indirect ELISA)
Figure 18.14b
Serological Tests
Figure 18.13
Serological Tests
• Direct tests detect antigens (from patient sample).
• Indirect tests detect antibodies (in patient′s serum).
Serological Tests• Agglutination: Particulate antigens• Hemagglutination: Agglutination of RBCs• Precipitation: Soluble antigens• Fluorescent-antibody technique: Antibodies
linked to fluorescent dye.
• Complement fixation: RBCs are indicator.• Neutralization: Inactivates toxin or virus.• ELISA: Peroxidase enzyme is the indicator.
Question 1
• Patient’s serum, influenza virus, sheep RBCs, and anti-sheep RBCs are mixed in a tube.
• Influenza virus agglutinates RBCs.
• What happens if the patient has antibodies against influenza virus?
Question 2
• Patient’s serum, Chlamydia, guinea pig complement, sheep RBCs, and anti-sheep RBCs are mixed in a tube.
• What happens if the patient has antibodies against Chlamydia?