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LAMPIRAN

Lampiran 1. Biodata Mahasiswa

Identitas

Nama : Husein Alaydrus

NIM : 22010111110140

Tempat/tanggal lahir : Semarang, 2 Januari 1993

Jenis kelamin : Laki-Laki

Alamat : Jl. Abdulrahman Saleh No. 116 Semarang

Nomor HP : 081327779830

e-mail : husein_alaydrus@ymail.com

Riwayat Pendidikan Formal

1. SD : SD Hidayatullah Lulus tahun : 2005

2. SMP : SMP Hidayatullah Lulus Tahun : 2008

3. SMA : SMAN 3 Semarang Lulus Tahun : 2011

4. FK UNDIP : Masuk Tahun 2011

Keanggotaan Organisasi

1. SENIOR HIMA KU Tahun 2011 s/d 2012

Lampiran 2. Ethical Clearance

Lampiran 3. Surat Izin Penelitian

Lampiran 4. Sodium Warfarin

Sodium warfarin simarc 2 mg

Komposisi:

Sodium warfarin 2 mg

Lampiran 5. Perhitungan dosis

Tabel 3. Konversi Dosis Pages dan Barnes

Hewan

dengan

dosis

diketahui

Hewan yang Dicari Ekivalensi Dosisnya

Mencit Tikus Marmot Kelinci Kucing Kera Anjing Manusia

20g 200g 400g 1,5g 2kg 4kg 12kg 70kg

Mencit 1,0 7,0 12,25 27,8 29,7 64,1 124,2 387,9

Tikus 0,14 1,0 1,74 3,9 4,2 9,2 17,8 56,0

Marmot 0,08 0,57 1,0 2,25 2,4 5,2 10,2 31,5

Kelinci 0,04 0,25 0,44 1,0 1,08 2,4 4,5 14,2

Kucing 0,03 0,23 0,41 0,92 1,0 2,2 4,1 13,0

Kera 0,016 0,11 0,19 0,42 0,45 1,0 1,9 6,1

Anjing 0,08 0,06 0,10 0,22 0,24 0,52 1,0 3,1

Manusia 0,0026 0,018 0,031 0,07 0,076 0,16 0,32 1,0

Lampiran 6. Hasil Statistik

Explore

Hati

NPar Tests

Kruskal-Wallis Test

Case Summaries

Hati

3 .3333 .41633 .2000 .00 .80

3 1.0000 .52915 1.2000 .40 1.40

3 1.9333 .11547 2.0000 1.80 2.00

9 1.0889 .77531 1.2000 .00 2.00

KelompokKontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.292 3 . .923 3 .463

.314 3 . .893 3 .363

.385 3 . .750 3 .000

Kelompok

Kontrol

LD50

LD100

Hati

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

Lilliefors Significance Correctiona.

Ranks

3 2.33

3 4.67

3 8.00

9

Kelompok

Kontrol

LD50

LD100

Total

Hati

N Mean Rank

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Test Statisticsa,b

6.543

2

.038

Chi-Square

df

Asymp. Sig.

Hati

Kruskal Wallis Testa.

Grouping Variable: Kelompokb.

Ranks

3 2.33 7.00

3 4.67 14.00

6

Kelompok

Kontrol

LD50

Total

Hati

N Mean Rank Sum of Ranks

Test Statisticsb

1.000

7.000

-1.528

.127

.200a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Hati

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Ranks

3 2.00 6.00

3 5.00 15.00

6

Kelompok

Kontrol

LD100

Total

Hati

N Mean Rank Sum of Ranks

NPar Tests

Mann-Whitney Test

Test Statisticsb

.000

6.000

-1.993

.046

.100a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Hati

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Ranks

3 2.00 6.00

3 5.00 15.00

6

Kelompok

LD50

LD100

Total

Hati

N Mean Rank Sum of Ranks

Test Statisticsb

.000

6.000

-1.993

.046

.100a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Hati

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Explore

Ukuran hati

Case Summaries

Ukuran hati

9 14.3900 1.24527 14.4700 12.60 16.58

9 13.3400 1.46925 13.5300 10.92 15.32

9 14.2289 1.91015 14.5500 10.70 16.92

27 13.9863 1.57651 13.7600 10.70 16.92

Kelompok

Kontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.138 9 .200* .976 9 .942

.178 9 .200* .925 9 .439

.133 9 .200* .969 9 .884

Kelompok

Kontrol

LD50

LD100

Ukuran hati

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

Test of Homogeneity of Variance

1.009 2 24 .379

.809 2 24 .457

.809 2 21.330 .459

.981 2 24 .389

Based on Mean

Based on Median

Based on Median and

with adjusted df

Based on trimmed mean

Ukuran hati

Levene

Statistic df1 df2 Sig.

Oneway

Explore

Berat hati

ANOVA

Ukuran hati

5.756 2 2.878 1.173 .326

58.864 24 2.453

64.620 26

Between Groups

Within Groups

Total

Sum of

Squares df Mean Square F Sig.

Case Summaries

Berat hati

9 6.5078 .81486 6.6000 5.40 7.68

8 5.0138 .85532 4.8900 4.07 6.64

9 4.1722 .51891 4.2200 3.37 5.11

26 5.2396 1.22839 5.1600 3.37 7.68

KelompokKontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.157 9 .200* .938 9 .558

.198 8 .200* .919 8 .420

.153 9 .200* .978 9 .955

Kelompok

Kontrol

LD50

LD100

Berat hati

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

Test of Homogeneity of Variance

1.496 2 23 .245

1.355 2 23 .278

1.355 2 21.138 .279

1.488 2 23 .247

Based on Mean

Based on Median

Based on Median and

with adjusted df

Based on trimmed mean

Berat hati

Levene

Statistic df1 df2 Sig.

Oneway

Post Hoc Test

Explore

Otak

ANOVA

Berat hati

25.136 2 12.568 22.965 .000

12.587 23 .547

37.723 25

Between Groups

Within Groups

Total

Sum of

Squares df Mean Square F Sig.

Multiple Comparisons

Dependent Variable: Berat hati

Bonferroni

1.49403* .35947 .001 .5659 2.4222

2.33556* .34873 .000 1.4351 3.2360

-1.49403* .35947 .001 -2.4222 -.5659

.84153 .35947 .085 -.0866 1.7697

-2.33556* .34873 .000 -3.2360 -1.4351

-.84153 .35947 .085 -1.7697 .0866

(J) Kelompok

LD50

LD100

Kontrol

LD100

Kontrol

LD50

(I) Kelompok

Kontrol

LD50

LD100

Mean

Difference

(I-J) Std. Error Sig. Lower Bound Upper Bound

95% Confidence Interval

The mean difference is significant at the .05 level.*.

Case Summaries

Otak

3 .0000 .00000 .0000 .00 .00

3 .0667 .11547 .0000 .00 .20

3 .2000 .20000 .2000 .00 .40

9 .0889 .14530 .0000 .00 .40

KelompokKontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

NPar Tests

Kruskal-Wallis Test

Tests of Normalityb

.385 3 . .750 3 .000

.175 3 . 1.000 3 1.000

Kelompok

LD50

LD100

Otak

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

Lilliefors Significance Correctiona.

Otak is constant when Kelompok = Kontrol. It has been omitted.b.

Ranks

3 3.50

3 4.83

3 6.67

9

Kelompok

Kontrol

LD50

LD100

Total

Otak

N Mean Rank

Explore

Ukuran otak

Test Statisticsa,b

2.889

2

.236

Chi-Square

df

Asymp. Sig.

Otak

Kruskal Wallis Testa.

Grouping Variable: Kelompokb.

Case Summaries

Ukuran otak

9 3.1522 .18740 3.1300 2.86 3.44

9 2.8089 .40334 2.6900 2.33 3.47

9 3.3078 .18130 3.3600 3.04 3.54

27 3.0896 .34072 3.1300 2.33 3.54

Kelompok

Kontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.137 9 .200* .967 9 .872

.171 9 .200* .935 9 .528

.194 9 .200* .912 9 .331

Kelompok

Kontrol

LD50

LD100

Ukuran otak

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

Test of Homogeneity of Variance

6.231 2 24 .007

3.574 2 24 .044

3.574 2 15.558 .053

6.047 2 24 .007

Based on Mean

Based on Median

Based on Median and

with adjusted df

Based on trimmed mean

Ukuran otak

Levene

Statistic df1 df2 Sig.

NPar Tests

Kruskal-Wallis Test

NPar Tests

Mann-Whitney Test

Ranks

9 14.39

9 8.56

9 19.06

27

Kelompok

Kontrol

LD50

LD100

Total

Ukuran otak

N Mean Rank

Test Statisticsa,b

7.917

2

.019

Chi-Square

df

Asymp. Sig.

Ukuran otak

Kruskal Wallis Testa.

Grouping Variable: Kelompokb.

Ranks

9 11.78 106.00

9 7.22 65.00

18

Kelompok

Kontrol

LD50

Total

Ukuran otak

N Mean Rank Sum of Ranks

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Test Statisticsb

20.000

65.000

-1.811

.070

.077a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Ukuran otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Ranks

9 7.61 68.50

9 11.39 102.50

18

Kelompok

Kontrol

LD100

Total

Ukuran otak

N Mean Rank Sum of Ranks

Test Statisticsb

23.500

68.500

-1.503

.133

.136a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Ukuran otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Ranks

9 6.33 57.00

9 12.67 114.00

18

Kelompok

LD50

LD100

Total

Ukuran otak

N Mean Rank Sum of Ranks

Explore

Berat otak

Test Statisticsb

12.000

57.000

-2.519

.012

.011a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Ukuran otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Case Summaries

Berat otak

9 1.6533 .04093 1.6700 1.57 1.71

9 1.3733 .12400 1.4000 1.07 1.47

9 1.3289 .17617 1.3500 1.02 1.49

27 1.4519 .19030 1.4700 1.02 1.71

KelompokKontrol

LD50

LD100

Total

N Mean Std. Deviation Median Minimum Maximum

Tests of Normality

.214 9 .200* .926 9 .444

.252 9 .103 .749 9 .005

.233 9 .173 .852 9 .079

Kelompok

Kontrol

LD50

LD100

Berat otak

Statistic df Sig. Statistic df Sig.

Kolmogorov-Smirnova

Shapiro-Wilk

This is a lower bound of the true significance.*.

Lilliefors Significance Correctiona.

Test of Homogeneity of Variance

4.410 2 24 .023

3.639 2 24 .042

3.639 2 17.470 .048

4.083 2 24 .030

Based on Mean

Based on Median

Based on Median and

with adjusted df

Based on trimmed mean

Berat otak

Levene

Statistic df1 df2 Sig.

NPar Tests

Kruskal-Wallis Test

Ranks

9 23.00

9 9.50

9 9.50

27

Kelompok

Kontrol

LD50

LD100

Total

Berat otak

N Mean Rank

Test Statisticsa,b

17.410

2

.000

Chi-Square

df

Asymp. Sig.

Berat otak

Kruskal Wallis Testa.

Grouping Variable: Kelompokb.

NPar Tests

Mann-Whitney Test

NPar Tests

Mann-Whitney Test

Ranks

9 14.00 126.00

9 5.00 45.00

18

Kelompok

Kontrol

LD50

Total

Berat otak

N Mean Rank Sum of Ranks

Test Statisticsb

.000

45.000

-3.585

.000

.000a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Berat otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Ranks

9 14.00 126.00

9 5.00 45.00

18

Kelompok

Kontrol

LD100

Total

Berat otak

N Mean Rank Sum of Ranks

Test Statisticsb

.000

45.000

-3.584

.000

.000a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Berat otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

NPar Tests

Mann-Whitney Test

Ranks

9 9.50 85.50

9 9.50 85.50

18

Kelompok

LD50

LD100

Total

Berat otak

N Mean Rank Sum of Ranks

Test Statisticsb

40.500

85.500

.000

1.000

1.000a

Mann-Whitney U

Wilcoxon W

Z

Asymp. Sig. (2-tailed)

Exact Sig. [2*(1-tailed

Sig.)]

Berat otak

Not corrected for ties.a.

Grouping Variable: Kelompokb.

Lampiran 7. Daftar Gambar

Gambar 5. Makrokopis Hati Kontrol

\

Gambar 6. Makrokopis Hati LD-50

Gambar 7. Makrokopis Hati LD-100

Gambar 8. Diagram Makroskopis Hati

KONTROL

KONTROL

LD-50

LD-50

LD-100 LD-100

UKURAN HATI

BERAT HATI

Gambar 9. Makrokopis Otak Kontrol

Gambar 10. Makrokopis Hati LD-50

Gambar 11. Makrokopis Otak LD-100

Gambar 12. Diagram Makroskopis Otak

KONTROL

KONTROL

LD-50

LD-50

LD-100

UKURAN OTAK

BERAT OTAK

LD-100

Gambar 14. Mikroskopis Hati LD-100 Gambar 15. Diagram Mikroskopis Hati

Gambar 17. Mikroskopis Otak LD-100 Gambar 18. Diagram Mikroskopis Otak

TANDA-TANDA KERUSAKAN ORGAN

HATI, SKOR 2, NEKROSIS, PENGECATAN

HEMATOXILLIN-EOSIN PEMBESARAN

400X

MIKROSKOPIS HATI

TANDA-TANDA KERUSAKAN OTAK

SKOR 0, TAK DITEMUKAN TANDA-

TANDA PERDARAHAN PENGECATAN

HEMATOXILLIN-EOSIN

PEMBESARAN 400X

MIKROSKOPIS OTAK