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Evaluating E -C Water Systems ™ on Evaluating E-C Water ... · Verification and assessment of...

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Evaluating E-C Water Systems™ on three commercial penaeid facilities in Brasil, Panama & Ecuador Evaluating E Evaluating E - - C Water Systems C Water Systems on on three commercial three commercial penaeid penaeid facilities in facilities in Brasil Brasil , Panama , Panama & Ecuador & Ecuador ELCE Technologies …Redefining water…™ Natal, office in Alliance with VerdeAqua.Br Ltda. Rua …….Natal, Rio Grande do Norte, Brasil. [email protected] . .
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Evaluating E-C Water Systems™ onthree commercial penaeid facilities in

Brasil, Panama & Ecuador

Evaluating EEvaluating E--C Water SystemsC Water Systems™™ ononthree commercial three commercial penaeid penaeid facilities in facilities in

BrasilBrasil, Panama, Panama & Ecuador& Ecuador

ELCE Technologies …Redefining water…™Natal, office in Alliance with VerdeAqua.Br Ltda.Rua …….Natal, Rio Grande do Norte, [email protected]

..

Bio E-C Water Systems ®ELCE TechnologiesELCE TechnologiesELCE TechnologiesELCE Technologies…Water redefinedWater redefinedWater redefinedWater redefined…™

Generalities of our new technologies.-

Water, incredibly simple yet, extremely complex. To this date, we have not found a way to replicate it nor create it from scratch in the most advanced laboratories of the world. It is the base of all biological processes. It is the universal conductor where life sparks started millions of years ago on this planet. Combining complex organic molecules, and other nutrients to sustain the very first live organims and non-organic unicelular structures.

After many years of rigorous and sistematic testing we are ready to present E-C Water Systems® “Active Waters” or “energized water” , in its most latent and vigorous form. VerdeAqua Ltda. International Consulting Firm, especialized in environmentally ethical aquaculture, introduces our newest member with in our lines of Clean Technologies autorizaded for Organic Acuaculture in a commercial alliance with technology developer, Elce do Brasil Ltda: Bio EBio E --C Water SystemsC Water Systems ®®..

日日 本本 治治 水水 株株 式式 会会 社社

ELCE Water ActivatorELCE Water Activator

ECWSECWS®® Water SystemsWater Systems™™ The Inside Story :The Inside Story :

The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the The ceramic balls contained inside the ELCEELCEELCEELCEELCEELCEELCEELCE device rotate device rotate device rotate device rotate device rotate device rotate device rotate device rotate and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing and rub against each other due to the water flow passing through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several through the equipment. This friction generates several forms of electrical energy:forms of electrical energy:forms of electrical energy:forms of electrical energy:forms of electrical energy:forms of electrical energy:forms of electrical energy:forms of electrical energy:PyroelectricityPyroelectricityPyroelectricityPyroelectricityPyroelectricityPyroelectricityPyroelectricityPyroelectricity: Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out : Energy generated by electrons coming out of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of of the ceramics and produced by the difference of temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or temperature between the ceramics surface and water, or between the ceramicsbetween the ceramicsbetween the ceramicsbetween the ceramicsbetween the ceramicsbetween the ceramicsbetween the ceramicsbetween the ceramics´́́́́́́́ inner and outer areas.inner and outer areas.inner and outer areas.inner and outer areas.inner and outer areas.inner and outer areas.inner and outer areas.inner and outer areas.PiezoelectricityPiezoelectricityPiezoelectricityPiezoelectricityPiezoelectricityPiezoelectricityPiezoelectricityPiezoelectricity: This is created by the water pressure : This is created by the water pressure : This is created by the water pressure : This is created by the water pressure : This is created by the water pressure : This is created by the water pressure : This is created by the water pressure : This is created by the water pressure changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a changes when passing through the ceramics or by a pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.pressure change between the ceramics themselves.Frictional electricityFrictional electricityFrictional electricityFrictional electricityFrictional electricityFrictional electricityFrictional electricityFrictional electricity: This electricity is produced due to : This electricity is produced due to : This electricity is produced due to : This electricity is produced due to : This electricity is produced due to : This electricity is produced due to : This electricity is produced due to : This electricity is produced due to friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result friction between the ceramics themselves or as the result of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the of collision of the ceramics and the water and the activatoractivatoractivatoractivatoractivatoractivatoractivatoractivator’’ s body.s body.s body.s body.s body.s body.s body.s body.

日日 本本 治治 水水 株株 式式 会会 社社

ELCE Water Activator

About ELCE Ceramics:

Natural stone 100% (Hornfels)

Making 5 micron powder (5 micron), granulating and baking

Baking 1200 degree

Absorption rate 0%(non-absorption)

Specific gravity 2.6

Hardness of mores 6.8 ( as same as crystal)

Raw ore After bakedBefore bake 1mm Ceramics

ELCE Ceramics

日日 本本 治治 水水 株株 式式 会会 社社

Stop motion time

Electrokinetic Potential

Electric double layerSiO2

Water current

Ceramics

+-

SiO2

e-

Water current

no charge

---

SiO2

e-

Water current

positive chargePeltier    EffectHigh to Low heat

電流電流電流電流

--

++

+⊖SiO2 SiO2

Local heat

--- -

++++

Working motion time

E-C Water Systems™Electrokinetic Potential Processing system

ECWSECWS®® Esquema instalado Esquema instalado en micro alga en micro alga -- Modulo Modulo

recirculatorio recirculatorio ::

ECWS S-5 modo recirculatorio

Linea Agua

Alga

externa

ELC

E

S-5

Bioensayo 1

35 lts

Bioensayo 2550 lts

35 lts

F1

550 lts

F2

日日 本本 治治 水水 株株 式式 会会 社社

ELCE Water ActivatorELCE Water Activator

La La Tecnologia Tecnologia ECWSECWS®® Water SystemsWater Systems™™ ::

•VARIOS TESTS DIRIGIDOS

PermeabilidadElectroconductividadTemperaturaFricciònOsmosis InversaDiferenciales de Presiòn

••VARIOS TESTS DIRIGIDOSVARIOS TESTS DIRIGIDOS

PermeabilidadPermeabilidadElectroconductividadElectroconductividadTemperaturaTemperaturaFricciFricciòònnOsmosisOsmosis InversaInversaDiferenciales Diferenciales de de PresiPresiòònn

日日 本本 治治 水水 株株 式式 会会 社社

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1111amount of penetration wateramount of penetration wateramount of penetration wateramount of penetration water(L/h)(L/h)(L/h)(L/h)amount of drain water (L/h)amount of drain water (L/h)amount of drain water (L/h)amount of drain water (L/h)

RO pomp pressure(Mpa)RO pomp pressure(Mpa)RO pomp pressure(Mpa)RO pomp pressure(Mpa)

((((L/h)))) ((((Mpa))))

Installed ECWS®

0000

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80808080

100100100100

120120120120

raw (before) water(raw (before) water(raw (before) water(raw (before) water(μμμμS/cm)S/cm)S/cm)S/cm)

drain water (drain water (drain water (drain water (μμμμS/cm)S/cm)S/cm)S/cm)

passed (RO membrane) water(passed (RO membrane) water(passed (RO membrane) water(passed (RO membrane) water(μμμμS/cm)S/cm)S/cm)S/cm)

6   7   8   9   10   11   12   1   2   3   4   5   6   7   8

Installed ECWS®

-30000-30000-30000-30000

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Installed ECWS®

0000

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passed (RO membrane) water end toxin (EU/L)passed (RO membrane) water end toxin (EU/L)passed (RO membrane) water end toxin (EU/L)passed (RO membrane) water end toxin (EU/L)

raw (before) water end toxin (EU/L)raw (before) water end toxin (EU/L)raw (before) water end toxin (EU/L)raw (before) water end toxin (EU/L)

Installed ECWS®

日日 本本 治治 水水 株株 式式 会会 社社

Test A3 ELCE do Brasil - Aquatec / RN Technician in charge : Eduardo Godoy

Tested Area - Maturation :Date & time test: 01/07/07Date & time end of test:04/08/07

Objectives of the Bio essay:

Verification and assessment of ECWS® de ELCE Technologies influencing the rate of daily copula in four maturation tanks.

Test A3 ELCE do Test A3 ELCE do Brasil Brasil -- Aquatec Aquatec / RN / RN Technician in charge : Eduardo Technician in charge : Eduardo GodoyGodoy

Tested Area Tested Area -- MaturatiMaturatioon :n :Date & time test: 01/07/07Date & time test: 01/07/07Date & time end of test:04/08/07Date & time end of test:04/08/07

Objectives of the Bio essay: Objectives of the Bio essay:

Verification and assessment of ECWSVerification and assessment of ECWS®® de ELCE Technologies influencing the rate de ELCE Technologies influencing the rate of daily cof daily copulaopula in four maturation tanks.in four maturation tanks.

日日 本本 治治 水水 株株 式式 会会 社社

Materials & MMaterials & Meethodsthods Used:Used:

The selected (4) maturation tanks were prepared to receive the The selected (4) maturation tanks were prepared to receive the ““activated wateractivated water”” and and the controls (4) of similar age and equivalent were marked. A fithe controls (4) of similar age and equivalent were marked. A fifth tankfth tank was used as a was used as a reservoir to fill and reservoir to fill and ““activateactivate”” the combined volume of water (8.1 the combined volume of water (8.1 mts mts x 4) x 4)

To To ““activateactivate”” the water we used tow ECWSthe water we used tow ECWS®® model SVmodel SV--2, with an adjusted flow of 2, with an adjusted flow of 34.3 liters per minute. 34.3 liters per minute.

The comparison data of the bioThe comparison data of the bio--essay tanks and control tanks was registered daily essay tanks and control tanks was registered daily along with molting, mortality rate and maturing ripped females.along with molting, mortality rate and maturing ripped females.

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日日 本本 治治 水水 株株 式式 会会 社社

Spawning Females Spawning Females -- Materials & Methods :Materials & Methods :

The Spawning observations registered coveredThe Spawning observations registered covered egg fertilization,egg fertilization, raterate of of eclosieclosiòònn, , nauplii nauplii viability, viability, and and nauplii nauplii per femalesper females in collective in collective spawningsspawnings..

To To ““activateactivate”” the water in this test we used tow ECWSthe water in this test we used tow ECWS®® model SVmodel SV--2, adjusted to a flow 2, adjusted to a flow of 16.61 lit/min. The common spawning tank held 10.3 cubic meterof 16.61 lit/min. The common spawning tank held 10.3 cubic meters of water. This s of water. This represented exactly an activation of represented exactly an activation of 2.62.6 times the total volume as recommended by times the total volume as recommended by Elce Elce TechnologiesTechnologies™™

The comparison data from the bio essay and controls was registerThe comparison data from the bio essay and controls was registered as viable ed as viable naups naups per day and registries of mortalities and abortion were kept as per day and registries of mortalities and abortion were kept as well.well.

日日 本本 治治 水水 株株 式式 会会 社社

References to the volume and dimensions of the Bio essayReferences to the volume and dimensions of the Bio essay::

Every maturation tank was kept at an exact volume of de 8,1 cubiEvery maturation tank was kept at an exact volume of de 8,1 cubic meters.c meters.

Population before the bio essay: 337 total females in Population before the bio essay: 337 total females in ““activatedactivated”” tanks & 368 total tanks & 368 total females in control tanks.females in control tanks.

Population after the bio essay: 295 Population after the bio essay: 295 total females in total females in ““activatedactivated”” tanks tanks & 332 & 332 total total females in control tanks.females in control tanks.

Water parameters (hydrological & environmental ) matched in theWater parameters (hydrological & environmental ) matched in the bio essay tanks bio essay tanks and control tanks as the source water was the same.and control tanks as the source water was the same.

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日日 本本 治治 水水 株株 式式 会会 社社

Registered Observations:Registered Observations:

••Daily log of ripped females,Daily log of ripped females,••Tank number / codeTank number / code••Colored bar code Colored bar code ••Daily Daily naup naup productionproduction••Data initiatedData initiated••Data endedData ended

AVALIA‚ÌO DA PRODU‚ÌO DIçRIA DE NAUPLIOS POR TANQUE

TM 05 TM 12 TM 18 TM 200 0 0 00 0 0 0

0 0 0 1 Produ ‹o di‡ria de nauplios2 0 0 14 0 1 00 0 1 14 1 1 23 1 5

8 10 0 69 7

14 14 4 611 9 10 513 12 5 411 15 4 9

13 12 14 1310 10 7 1218 18 9 1217 13 7 915 8 9 69 15 1 9

010 19 9 613 18 7 713 16 4 14

8 21 7 14 A B13 21 8 11

10 20 5 215 19 6 1314 10 6 7

TM 05 TM 12

Desovas

497,889 00 0

1,327,704 1,778,8101,493,667 1,245,1672,323,482 2,490,3341,825,593 1,600,9292,157,519 2,134,5721,825,593 2,668,215

0 02,157,519 2,134,5721,659,630 1,778,8102,987,334 3,201,8582,821,371 2,312,4532,489,445 1,423,0481,493,667 2,668,215

0 01,659,630 3,379,7392,157,519 3,201,8582,157,519 2,846,096

0 01,327,704 3,735,5012,157,519 3,735,501

0 01,659,630 3,557,6202,489,445 3,379,739

40,992,861 51,051,847

2,323,482 1,778,810

日日 本本 治治 水水 株株 式式 会会 社社

Observations:Observations:

A 24.5%

B 60.8%15,932,44840,992,861 51,051,847

25,614,864

Results and Conclusions of the Bio essay:Results and Conclusions of the Bio essay:

0.0

5.0

10.0

15.0

20.0

25.0

Desovas

The net gain with The net gain with ““Activated waterActivated water”” using the using the ECWSECWS™™ for viable for viable nauplii nauplii was of was of ++60.8%60.8% over the over the total period using the activators in the Activated total period using the activators in the Activated system system vs vs the Controlthe Control

Metodology Metodology ::

* 100%* 100% user user friendlyfriendly

* 100% Eco* 100% Eco

* 100%* 100%CostCost--EffectiveEffective

* 100%* 100% technically technically supportedsupported

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日日 本本 治治 水水 株株 式式 会会 社社

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Test de ELCE do Test de ELCE do Brasil Brasil -- Aquatec Aquatec / RN / RN Technician in Charge : Eduardo Technician in Charge : Eduardo GodoyGodoy

Tested area Tested area -- External Algae production :External Algae production :Date & time of test: 01/07/07Date & time of test: 01/07/07Date & time of end of test : 04/08/07Date & time of end of test : 04/08/07

Objectives of the Bio essay: Objectives of the Bio essay:

Assessment of the performance of the ECWSAssessment of the performance of the ECWS®® activator by ELCE Technologiesactivator by ELCE Technologies™™influencing growth rates of the micro alga influencing growth rates of the micro alga thalassiosira sppthalassiosira spp..

ECWS® by ELCE TechnologiesECWSECWS®® by ELCE Technologiesby ELCE Technologies

日日 本本 治治 水水 株株 式式 会会 社社

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Materials and Methods used in the Bio Essay:

Two pet translucent sacs holding 35 liters of activated water were marked and used along with two control 35 liters bags inoculated at the same time and with a common initial source water

To “activate” the water we used 1 activator ECWS® model S-5, adjusted to a continuous flow of 16.61 lit / min

Daily entries in the count log were registered as a function for algae reproduction and growth as cells per ml.

Materials and Methods used in the Bio Essay:Materials and Methods used in the Bio Essay:

Two pet translucent sacs holding 35 liters of activated water weTwo pet translucent sacs holding 35 liters of activated water were marked and used re marked and used along with two control along with two control 35 liters bags inoculated at the same time and with a common 35 liters bags inoculated at the same time and with a common initial source waterinitial source water

To To ““activateactivate”” the water we usedthe water we used 11 activator ECWSactivator ECWS®® model Smodel S--5, adjusted to a 5, adjusted to a continuous flow of 16.61 lit / mincontinuous flow of 16.61 lit / min

Daily entries in the count log were registered as a function forDaily entries in the count log were registered as a function for algae reproduction and algae reproduction and growth as cells per ml.growth as cells per ml.

Normal waterNormal water

Activated waterActivated water

日日 本本 治治 水水 株株 式式 会会 社社

References and Volumes of the Bio EssayReferences and Volumes of the Bio Essay::

The bio essays were ran in parallel. Two sets of 35 The bio essays were ran in parallel. Two sets of 35 lts lts in pet translucent bags in pet translucent bags (activated and controls) & two sets of 550 (activated and controls) & two sets of 550 lts lts in transparent fiber glass cylinders in transparent fiber glass cylinders (activated and controls).(activated and controls).

Initial Initial inoculum inoculum in pet sacs of 35 in pet sacs of 35 lts lts was : 215.000 cell/ml. On 550 was : 215.000 cell/ml. On 550 lts lts tanks with tanks with activated water & 45.000 c/ml. 237.500 c/ml & 60.000 c/ml respecactivated water & 45.000 c/ml. 237.500 c/ml & 60.000 c/ml respectively in sacs and tively in sacs and control tanks. Evaluation period Period 96 hrscontrol tanks. Evaluation period Period 96 hrs

Population after bio essay: 35 Population after bio essay: 35 lts lts -- 890.000 c/ml 550 890.000 c/ml 550 lts lts -- 230.000 c/ml total in sacs 230.000 c/ml total in sacs and tanks with activated water. 745.000 c/ml and 270.000 c/mland tanks with activated water. 745.000 c/ml and 270.000 c/ml in control tanks in control tanks respectively. respectively. Period 96 hrsPeriod 96 hrs

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日日 本本 治治 水水 株株 式式 会会 社社

Test de ELCE do Test de ELCE do Brasil Brasil -- Pacific Larval Centre / Panama Pacific Larval Centre / Panama Technician in Charge : GerTechnician in Charge : Geròònimo Nunimo Nuññezez

Area Tested Area Tested -- Analysis of Micro alga :Analysis of Micro alga :Date and time test: 11/04/02Date and time test: 11/04/02Date and time test end: 30/04/02Date and time test end: 30/04/02

Objectives of the Bio Essay: Objectives of the Bio Essay:

Proper assessment of the ECWSProper assessment of the ECWS®® activator by ELCE Technologies would influence activator by ELCE Technologies would influence / modify the nutritional profiles of the micro algae / modify the nutritional profiles of the micro algae chaetoceros mullerii chaetoceros mullerii and compare and compare the nutritional over all performance of the the nutritional over all performance of the ““activatedactivated”” micro algae on post larval micro algae on post larval development and survival rates.development and survival rates.

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日日 本本 治治 水水 株株 式式 会会 社社

LAISA LAISA -- Laboratorios Laboratorios de de Analisis Industriales Analisis Industriales S.A. / S.A. / Cdad Cdad de Panama, Panama de Panama, Panama Technician in charge : Prof. Technician in charge : Prof. Isaias Isaias CamachoCamacho

Results : In c/100gResults : In c/100gNormal algaeNormal algae ““Activated AlgaeActivated Algae””

1.1. Fatty acids:Fatty acids:Ac. Ac. CaprilicoCaprilico 53.453.4 97.797.7Ac. Ac. CaproicoCaproico 24.824.8 ------------Ac. Ac. MiristicoMiristico 21.821.8 1.71.7 AOACAOAC--Gas C.Gas C.

2.2. CarotenoidsCarotenoids 1280 1280 ugug 1850 1850 ugug� AOAC � AOAC -- 970.64970.64

3.3. ChlorofilaChlorofila 1420 mg 1420 mg 1780 mg AOAC 1780 mg AOAC -- 940.03940.03

Results : We obtained up to 83% increment in the fraction of fattyAcids. An impressive 44.5% carotenoids and a 24.5% in chlorofil by an independent accredited laboratory. This indexes could have influenced positively the larval growth, uniformity and survival rates with in the LRT’s “fed on activated algae” vs the control LRT’s as presented in summary chart A2

日日 本本 治治 水水 株株 式式 会会 社社

Teste Teste de ELCE do de ELCE do Brasil Brasil -- Aquatec Ltda Aquatec Ltda -- Brasil Brasil Technician in charge : Eduardo Technician in charge : Eduardo GodoyGodoy

Tested area Tested area -- Larviculture Larviculture F 2 :F 2 :Date and time of test: 11/04/02Date and time of test: 11/04/02Date and time of end test: 30/04/02Date and time of end test: 30/04/02

Objectives of the Bio Essay: Objectives of the Bio Essay:

Proper assessment of the activator ECWSProper assessment of the activator ECWS ®® by ELCE Technologies would by ELCE Technologies would influence positively larval development profiles. influence positively larval development profiles. Branchial Branchial development, development, uniformity, and survival.uniformity, and survival.

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日日 本本 治治 水水 株株 式式 会会 社社

Materials and Methods :Materials and Methods :

External phase two (F2) raceways 55 tons in capacity, liner coveExternal phase two (F2) raceways 55 tons in capacity, liner covered (red (pvcpvc))

Initial stocking : 85 Initial stocking : 85 pls pls per liter (pl 3per liter (pl 3--4) Stock at harvest : 80 4) Stock at harvest : 80 pls pls per liter (pl 12per liter (pl 12--13)13)

ECWS model SECWS model S--

Results : We obtained up to 98% uniformity (2 sizes) plResults : We obtained up to 98% uniformity (2 sizes) pl--8 8 vs vs an 81% on the control an 81% on the control raceways. In the observations of raceways. In the observations of branchial branchial development in activated systemsdevelopment in activated systems (A (A RwyRwy)) presented 60%presented 60% of the larvae (plof the larvae (pl--8) 8) with more than 4 with more than 4 branchial lamelae vs branchial lamelae vs 44% 44% in the control at the same age and stage.in the control at the same age and stage. Lesser Lesser consumptionconsumption of diets (of diets (--16%) No 16%) No ““foamingfoaming”” action in the surface of the raceways with activated water usinaction in the surface of the raceways with activated water using ECWSg ECWS®®

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Norma waterNorma water Activated water”Activated water”

日日 本本 治治 水水 株株 式式 会会 社社Bio EBio E--C Water Systems by ELCE TechnologiesC Water Systems by ELCE Technologies

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 Activated water Surface tension reduction

•Redox potential changes. •Formation of salt crystal nucleous in water. •Active permeability of water molecules through the porous structure of hard surface lodging.

•Formation of a protective anticorrosive film on metal surface.

•High penetrating capability through cell membranes in living organisms and plants.

•High sanitary capability. •Deodorizing effect. •Retention of chemical properties and composition of natural water.

 Activated water Surface tension reduction

•Redox potential changes. •Formation of salt crystal nucleous in water. •Active permeability of water molecules through the porous structure of hard surface lodging.

•Formation of a protective anticorrosive film on metal surface.

•High penetrating capability through cell membranes in living organisms and plants.

•High sanitary capability. •Deodorizing effect. •Retention of chemical properties and composition of natural water.

Summary:Summary:BioEssays BioEssays by ELCE by ELCE Technologies Technologies ™™

Bio E-C Water Systems ¨ EEEELCLCLCLC EEEE TeTeTeTe chnchnchnchn oooo llll ogogogog iiii eseseses…Wat…Wat…Wat…Wat erererer rererere ddddeeee finfinfinfin eeee d…™d…™d…™d…™

ELCE WATER ACTIVATING SYSTEMS

TEST E-C Water Systems® - Internacional

Brasil : Aquatec, Natal, RN

Panama : Pacific Larval Centre Inc Ecuador : Sularva S.A.

RESUMEN / RESULTADOS :

TEST COPULA - DIA :

* TM equivalentes e/p Brasil Ecuador Panama Otros

Control 6% 9% nd

Agua Activada AA 10.80% 12% nd

TEST Nuplio x hembra

* ( 1000)

Control 155 140 nd

Agua Activada AA 201 190 nd

TEST Micro algas :

* 35 lts thalassiosira spp.

Control 214% 198% 205%

Agua Activada AA 314% 297% 320%

TEST Micro algas :

* 550 lts thalassiosira spp.

Control 350% 375% 320%

Agua Activada AA 411% 400% 405%

TEST de Uniformidad:

* Rango 2 clases

Control 81% 78% 70%

Agua Activada AA 98% 95% 93%

TEST de Desarrollo Branquial:

* 4 p pl 8

Control 36% 41% 60%

Agua Activada AA 52% 55% 80%

* l. vannamei

* AA = Agua Activada info contacto : [email protected]

* NA = Agua No Activada Gerònimo Nuñez - PLC - Panama

Porcentual de Copula Dia

* Cria larvaria F -1 & 2 Mostraron 12% & 16% respectivamente menor oferta de alimento

* Cria larvaria F- 2 Mostraron 22% mejor performance al test stress & mayor actividad

Conteo / % sobre inoculo

Conteo / % sobre inoculo

Porcentual / Tamanho (mm)

% 4 pares - pl 8

Opinion:Opinion: Studies by ELCE Studies by ELCE Technologies Technologies ™™

� There is a direct relationship in the funcionality ofthe ECWS® units used that affected positively ALL of the bio essays conducted in all aquaculture centers ( Aquatec Ltda, Sularva S.A. & PLC S.A.)

� ECWS ® stimulates daily copula, nauplii viability, quality and quantity of

the two micro algae used in the larval diet, uniformity, development and

survival of the larva.

� ECWS ® affects positively the culture environment preserving for longer

periods the quality of the feeds in the maturation and larval rearing

facilities maduraciòn with an important 18% reduction in offerings due to

slower fouling and leaching of the nutrients.

�� There is a direct relationshipThere is a direct relationshipin the in the funcionality funcionality ofof the ECWSthe ECWS®® units used units used that affected positivelythat affected positivelyALL of the bio essaysALL of the bio essaysconducted in all aquaculture conducted in all aquaculture centers ( centers ( Aquatec LtdaAquatec Ltda, , Sularva Sularva S.A. & PLC S.A.)S.A. & PLC S.A.)

�� ECWS ECWS ®® stimulates daily copula, stimulates daily copula, nauplii nauplii viability, quality and quantity of viability, quality and quantity of

thethetwo micro algae used in the larval diet, uniformity,two micro algae used in the larval diet, uniformity,developmentdevelopmentand and

survival ofsurvival ofthe larva.the larva.

�� ECWS ECWS ®® affects positivelyaffects positivelythe culture environment preserving forthe culture environment preserving forlonger longer

periods theperiods thequality ofquality of the feeds in the maturation and larval rearing the feeds in the maturation and larval rearing

facilities facilities maduracimaduraciòòn n with an important 18% reduction in offerings due to with an important 18% reduction in offerings due to

slower fouling and leaching of the nutrients.slower fouling and leaching of the nutrients.


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