Evaluation of sisal post-harvest waste as a potential bioresource for production of oyster mushrooms (Pleurotus sapidus (P969) and

Pleurotus HK-37)

ANTHONY MSHANDETE-UDSM

First Bio-Innovate Regional Scientific Conference, Addis Ababa, Ethiopia, 25-27 Feb 2013

From sisal post-harvest wastes to edible valuable mushrooms crop

BackgroundBIOREFINERY: is the technology for Biomass bioresource conversion into useful biomaterials and/or bio-energy carriers in an integrated manner and thereby it can maximize the economic value of the biomass used while reducing the waste streams produced

From sisal post-harvest wastes to edible valuable mushrooms crop

Challenges to BiorefineryOne of the main challenge; Sustainability of biomass resources supply being is ONE of the key issues for the transition towards the bio-based economy. Therefore the biomass resources needs to be identified from the perspective of supply and demand.

From sisal post-harvest wastes to edible valuable mushrooms crop

What is the problem?

Scarcity of innovative sustainable technologies and their adoption for mushroom production from sisal processing industry in Tanzania particularly SPHW contributes to environmental pollution, emission of GHG and biological resource wastage.

From sisal post-harvest wastes to edible valuable mushrooms crop

Demonstrated potential of SPHWSisal post harvest wastes has been identified quantified and characterized as a bioresource. SISAL INDUSTRY: Generically discards 98% of sisal plant biomass as waste

From sisal post-harvest wastes to edible valuable mushrooms crop

Sisal post harvest wastes bioresource.

SISAL LEAF REMNANTS WASTE SISAL BOLE WASTES

SPHW EACH WEIGHS 19=129 KG

From sisal post-harvest wastes to edible valuable mushrooms crop

Sisal post harvest wastes bioresourceMshandete, A.M., Kibazohi, O and Kivaisi, A.K., (2013). Tanzania sisal industry: Auditing and characterization of sisal post-harvest wastes as a bioresource for biorefining. International Journal of Pure and Applied Sciences and Technology. (IN PRESS). Proposed integrated utilization of sisal post-harvest waste. SEE OVERLEAF

Biofertilizer to sisal plantations

Sisal Post-harvest waste Sisal boles including leaf stubs

Chopping, crushing, leachate extraction

Biomass residue

Leachate

Chemical/fermentation processing

Inulin, organic acids, ethanol, biogas, etc.

Chopping, drying, grinding

Poles

Construction materials

Mushroom cultivationMushrooms: To markets

Spent mushroom substrate

Co-digestion with animal manures for biogas Biogas

From sisal post-harvest wastes to edible valuable mushrooms crop

Technical limitations of SPHW for mushroom cultivation.

SISAL LEAF REMNANTS WASTE SISAL BOLE WASTES

From sisal post-harvest wastes to edible valuable mushrooms crop

How Technical limitations were ADRESSED:

*Pre-treatment in water by complete immersion (fermentation) for 10 days

*The pre-treated SPHW was used as substrate fabricated glass SSF bioreactors

SPHW formulation for mushrooms cultivation in glass bioreactors

•THREE SUBSTRATES:RSL (DRIED);SBW (DRIED) MIXTURE RSL (70%):SBW(30%)

•FIVE SPAWN RATES % (1.5, 2.5, 4, 5 AND 6.5) 200g moist substrate per glass column• •TWO OYSTER MUSHROOMS (PLEUROTUS HK37,PLEUROTUS SAPIDUS P 969)

•Volume 500 ml, Height 27 cm, Inner diameter 4.7 cm (r=2.35cm).•Volume (cm3) occupied by mycelia=3.14xr2xhDensity =mass (g) of spawn/volume (g/cm3)

Colonization rate (mm/day)

SPHW formulation for mushrooms cultivation in I kg plastic bags

*BEST MUSHROOM SEEDS rates of 2.5 and 4% of Pleurotus HK-37*Mixture 30% SBW and 70% SLR *BE of 80-86% *Mushroom yield of 251-270 g/kg moist substrate

Conclusions*Mixed SPHW may be considered a novel substrate for large-scale Pleurotus HK-37 production

*For mushroom commercial ventures bioconversion of 25% of the moist substrate into fresh mushrooms is considered profitable.

*Based on Mushroom yield of 250 g/kg moist substrate (25%), for large scale 2000 bags each 2 kg can produce 1000 kg of fresh mushrooms per month.

Pathways to impact*Knowledge and skills transfer to mushrooms growers and entrepreneurs UDSM has TWO manuals

“Department receives the International Linnaeus Tricentennial Diploma from Her Majesty the Crown Princes Victoria of Sweden, Outstanding Mycological Research of benefit to Society” Award, Sweden

Pathways to impact*Production of high quality MUSHROOM SEEDS MATERIALS FOR GROWERS IN TANZANIA

MUSHROOM CULTURE

MUSHROOM SEEDS OYSTER MUSHROOMS

Acknowledgement: Bio-Innovate Regional Scientific Conference –UNECA, Addis

Ababa