Date post: | 21-Dec-2015 |
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Abortive initiation assay
Let R = RNA polymerase, P = promoter (closed), and Po= promoter (open)
R + P RP RPoATP + UTP*
ApUp*U
kf
kr
KB
Abortive transcripts
[ApUp*U]
time
lag
Measure kf and KB from lag time vs. 1/[R]
Lag time
Lag time in abortive initiation assay is inversely proportional to [R].
Lag time = KB kf
1
kf
1x +1
[R]
1
[R]Y-intercept = kf
1
Slope = KB kf
1
Mutations in the δ subunit of RNA polymerase that interfere w/λ repressor-mediated activation of PRM transcription
Effect of mutations in the δ subunit of RNA polymerase on activator-dependent and independent transcription of the lac
promoter