Mara KettleRocheSummer 2012

Skills Learned• General Mouse Lab Procedure

and Skills-including husbandry• Experimental Preparation• Experimental Design• Data Collection and Analysis• Scientific Literature• Professional Social Skills

General Mouse Lab Procedure and Skills Proper Handling of Mice

Maintenance Sexing

Behavioral Tests Formalin Test Hot Plate Test Social Interaction

Experimental Preparation* Observed an on-going pain experiment

Formalin Pain Test and Social Interaction

Preliminary Literature Search Pubmed Academic Search Complete

(EBSCOhost.com) Helped Design an Experiment

Short Term Pain Study-Major Focus of my Work

Hypothesis

Stress decreases pain response because it triggers a pain suppression response called SIA

Necessary Components Aversive Stimuli: Stressors:

Restraint (severe) Noxious Pain Stimuli: Pain Test: Hot

Plate

What is SIA?

“a phenomenon of pain suppression that occurs upon exposure to stressful stimuli”1

Accidents

Wars/Battles

Trauma

Experimental Design

16 Male Mice: BTBR T+ tf/J (n=8) C57 BL/6J(n=8)

Restraint Stress

Hot Plate Test

Strains of Mice Used

BTBR C57•An autistic model•Show reduced social interactions, impaired play, low exploratory behavior, and high anxiety

• Used as a control/general purpose strain •Breeds well, long life-span

Experimental Design (cont.)1. Pre-stress pain test

2. Give mice stress Restraint stress for an hour in clear 50ml

centrifuge tubes

3. Post-stress pain test

Experimental Methods• Pre Stress Testing

• Cages were housed outside testing room

• Mice were placed onto hotplate heated 53°C and latency until first pain behavior was measured

• Pain latency was recorded and procedure was repeated until all mice were tested

Restraint Stress

• Method • 50ml centrifuge tubes

• 1 hour duration

• Post-stress testing began immediately following

• post stress pain latency time recorded

Results

P<0.05

P<0.05

Results cont…

Conclusion:

Restraint stress increases hotplate latencies in C57 and BTBR mice

It appears that there is a strain by treatment effect ;C57 mice demonstrate a greater stress induced analgesia”

References

1 Butler RK, Finn DP. Stress-induced analgesia. Prog Neurobiol. 2009 Jul;88(3):184-202. Epub 2009 Apr 22. Review. PubMed PMID: 19393288.

Acknowledgements

Roche ProgramWilliam Paterson UniversityDr. WeissMr. Norman SchanzDr. BennoAbbie Post (mentor) Joon Ho Seo (consultant)

Thank You