Introduction
What is the traditional developing method?
What is experimental design?
What do we need and what kind of possibilities do we have for designing?
Why is it useful during product development?
Where and how can we use or apply the design results?
Traditional method
Developing a hypothesis
Conducting an experiment to test the hypothesis
Modifying the hypothesis on the basis of the experiment results
Conducting an additional experiment based on the modified hypothesis
Drawbacks of the traditional method
Limited understanding of variable effect
No information about interaction with other
components
The approach is extremely inefficient
Requires too many experiments and
too much time
Expensive
The developer must work until ...
a satisfactory result is found
all possibilities are exhausted
all available time and money are exhausted ...
Because the ...
data processing is slow
the number of errors is high
What kind of possibilities do we have?
Data from previous investigations
Experience, knowledge and intuition
Computer software
neural networks
designing models
Signal transport between neurons
Eletrical signalAxon
Electrical signal
Presynaptic membrane
Postsynapticmembrane
Synaptic gap
Neurotransmittermaterial
Relationship between biological and artificial neural network system (ANN)
Treshold value
LinearTresholdgate
What is artificial neural network?
A modelling tool that discovers a relationship from a database of examples
An automatic mathematical construction method for modelling preparations directly from data
A cost-effective possibility for designing a new preparation
What is needed for design?
Pre-examination results of the substances to be used
Know the final dose or the final pharmaceutical form
Determination of preparation parameters
Test results of the preparation
Application areas of ANN
Pattern recognition Economic and social models Financial sector
Marketing modeling Optimization of investments
Telecommunication Signal Analysis Data compression
Environmental Protection Weather forecast
Biology
Industry Quality control Manufacturing Planning Fault Diagnosis Combine multiple source data
General characteristics of ANN1. It consists of nodes and links between the knots
the weighted input of the input signals is calculated
the amount is compared with the threshold(s)
linear or nonlinear transmission function
their "behavior" changes their behavior and the links between the knots
2. It can be divided into three main parts an interconnected network of nodes
the node activation rule
the learning rule for nodes
Applied ANNsAssociating networksFeature extracting networksNonadaptive networks
Back-propagation model
Bayesian network
(Generalized Regression Neural Network)
Input layer
Hidden layerRegression layer
Output layer
Other applied ANNsBox-BehnkenCentral compositePseudo-randomResilient Propagation (Rprop)Resilient Propagation with MAximum-Posterior (Rprop-MAP)
Algorithm: back-propagation
Determining the number of hidden neurons
M.N. Jadid et al: Eng.Appl.Artif.Intell. 9 (1996) 303-319
J.C. Carpenter et al: AI Expert 10 (1995) 31-33
The optimization of the node number of the "hidden" layer depends on:
the number of inbound and outbound neurons
the number of data used to teach
the "noise level" of the desired value
the complexity of the function you want to teach
the type of neural network
from the activation value of hidden nodes
from the teaching algorithm
How does a neural network work?
Data collection
Determination of variables and parameters
Data input into the computer
Teaching and checking
Application of knowledge
What do we need?
Input parameters Output parameters
effective materials
disintegrants
fillers
binders
glidants
lubricants
etc.
particle size
flow properties
hardness
friability
disintegration time
dissolution rate
etc.
Predicted and experimental dissolution valuesY1 Y2 Y3 Y4
P E P E P E P E
F1 47,00 47,03 74,66 74,69 92,61 92,62 103,4 103,4
F2 21,38 21,36 47,17 47,16 91,38 91,42 102,3 102,3
F3 13,81 13,81 21,61 21,61 33,95 33,94 47,49 47,48
F4 17,03 17,03 26,23 26,22 47,02 46,99 67,21 67,19
F5 14,06 14,06 21,81 21,81 32,86 32,85 49,55 49,54
F6 23,12 23,12 50,35 50,36 80,40 80,44 99,16 99,19
F7 17,02 16,66 30,08 29,81 49,79 49,87 67,44 68,76
F8 17,02 17,37 30,08 30,35 49,79 49,71 67,44 66,11
F9 14,47 14,46 26,46 26,44 42,44 42,41 62,08 62,06
F10 36,11 36,11 65,80 65,80 77,18 77,17 99,43 99,43
Response surfaces of the influence of the percentage of Eudragit L 100 and tablet hardness on the percentage of ASA released after (A) 1 hour, (B) 2 hours, (C) 4 hours, and (D) 8 hours, predicted using the GRNN.
Contour plots of the influence of percentage of Eudragit L 100 and tablet hardness on percentage of ASA released after (A) 1 hour, (B) 2 hours, (C) 4 hours, and (D) 8 hours, predicted using the GRNN.
What does experimental designinvolve?
A practice that employs statistical tools and
methods in scientific experimenting
Variables via which we are attempting to make a
correlation or regression with a measurable input
and output we are trying to predict
What is necessary for designing
Preliminary investigations of the materials
The dosage and the dosage form of the final product
Determination of the parameters of the preparation process
Results of the investigations of the preparations
Design method selection
Central compositeFace centeredBox-BehnkenSimplexEquiradialRandom
Simplex centroidSimplex LatticeHybrid
Material and method
API: Dilthiazem HCl (Dilt)Filler: Vivapur 101 (V101)Disintegrant: Era-Tab (Era-T)Binder: Pharmacoat 603 (P603)
Granule:
Desing mode: Hybrid design
Preparation: Freund CF-360 granulator
Design the „core”
Min. 100 g
Max. 500 g
Min. 300 g
Max. 500 g
Min. 100 g
Max. 500 g
Min. 10 g
Max. 40 gP603
Dilt
Era-T
V101
Designed compositions
Dilt Era-T V101 P603
(g) (g) (g) (g)
MST-1 315 339 346 23
MST-2 144 440 417 36
MST-3 471 413 116 17
MST-4 426 408 166 23
MST-5 374 453 173 19
MST-6 342 378 280 23
MST-7 365 420 215 21
Preparation conditions
Liqu. add. Rotor Temp
ml/min rpm °C
MST-1 10 140 45
MST-2 10 140 45
MST-3 15 160 45
MST-4 15 160 55
MST-5 20 200 55
MST-6 20 200 65
MST-7 20 200 65
Investigated properties
1,649 1,19 0,751 0,15
1,753 1,17 0,747 0,12
1,583 1,29 0,729 0,18
1,651 1,16 0,746 0,23
1,678 1,25 0,757 0,31
1,731 1,22 0,781 0,17
1,939 1,19 0,731 0,19
Density Roundness Hardness Friability
(N) (%)
MST-1
MST-2
MST-3
MST-4
MST-5
MST-6
MST-7
Area Application Applied DoE TYPE
Oral drug delivery Tablet formulation development Multivariate design (fractional factorial design in14 variables, 214–9 design, 35 experiments)
Multivariate design + simplex optimization byModde Optimizer
Fractional factorial designs (two studies); design space definition using a simplified BayesianMonte Carlo simulation
Mixture design
Oral drug delivery –immediate release (IR)
Dispersible tablets development Several factorial experiments at 2–3 factors, 2–3 levels
Immediate release tablet platform Resolution V 25–1 fractional factorial design
Fast dissolving pellets 25–1 fractional factorial design, five factors (fournumeric and one categorical), two levels
Oral drug delivery –modified release (MR)
Gastroretentive dosage form 3-level-3-factor, Box–Behnken design
Areas where it is useful
Area Application Applied DoE TYPE
Inhalation drug delivery Powder for inhalation(formulation and process development)
Half-fractional factorial design with five factors attwo levels with resolution V
Face centered central Composite Design withthree factors at three levels
Risk assessment by Lean QbD Software
Transdermal drug delivery Patch development 24 full factorial design
Iontophoretic delivery Face-centered central composite design (totalnumber of experimental combinations 2k +2k + n0, with k = number of independent variables and n0
= number of repetitions of the experiments at thecenter point)
Cutaneous drug delivery (Topical) Nanoemulsion for leishmaniasis (formulation development)
22 full factorial design
Microsponge-based gel for surgical wounds (development)
3-factor, 3-level Box–Behnken design
Ocular drug delivery PEGylated PLGA nanospheres (optimization and
characterization)
Central composite factorial design
Liquid crystalline nanoparticles (formulation
optimization)
Fractional factorial design 25–1; simplex-lattice
experimental design
Area Application Applied DoE TYPE
Injections Formulation for parenteral nutrition
(development)
D-optimal experimental design (mixture design)
Biopharmaceuticals Toward QbD implementation in the
biopharmaceutical industry
QbD for biopharmaceuticals
QbD for risk assessment and management (Case
Study on Monoclonal Antibody)
Antibody Formulation Robustness Multivariate study (full factorial) including three
factors at two levels
Protein formulation
Nanopharmaceutics Development and industrialization of polymeric
targeted nanoparticle drug delivery platforms
Solid Lipid Nanoparticles for Inhalation (process
development)
Two-level full factorial design (with no center
points and three repetitions for each level)
Area Application Applied DoE TYPE
Pharmaceutical processes Dry powder inhaler capsule filling D-optimal model with design statistics G-
efficiency with three replicates
Excipient micronization Full factorial (three variables at two levels, eight
runs)
Freeze-drying of injectables Design space calculation (according to a cited
model)
Film-formation by spraying Rechtschaffner Res V two-level fractional design
for four variables with center point
Nano-precipitation and nanospray-drying Design model based on integrated-variance
optimal design for surface response
Analytical methods Method (e.g. chromatographic) development
Test methods Adhesion test (for patches) Randomized response surface design, five
factors, 38 runs
In vitro aerosol deposition in human cast (for
inhaled products)
Half-factorial design
Generic drug products Generic product equivalence Fractional factorial design with triplicate center
points
Conclusions
It is necessary to know the properties of the materials
The dosage form and the final dosage of the preparation must be known for the preliminary investigations
It is possible to determine the optimal parameters of the preparation process
It can generally be used for the development of new products