Experimental Evolution

Silke & Elsa

Hopkins Microbiology

• Selection for fit types in a variable population

• Selection pressure: antibiotic

• Mutants survive

• Are NalR mutants less fit in an antibiotic-free environment? Tradeoff?

• Select for NalR mutants (different Nal-Conc) correlation between selective pressure and number of mutants?

• Nucleotide sequence of candidate genes Mutations?

• Recovery of fitness during many generations when selection removed? Duration?

Suggestions:

Maintain selective pressure for NalR Mutant up to “cage fight” ?

Plate replicates and/or various timepoints