Extractibles forestiers comme agent antimicrobien :
mise en place d’une chaîne de valeur au Lac St-Jean pour leur exploitation
Nathalie Bourdeau, Patrick Marchand et collaborateurs
2
Who we are?
Why forest extract?
How to obtain a commercial antimicrobial extract from
wood bark ?
Some results of our research
Who we are?
Under the same roof
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Fondamental and applied research on biobased products, biomass pretreatment, vegetal biology and metabolomic
$80M pilot plant
More than 100 industrial partners
Industrial ResearchChair on Environment
and BiotechnologyResearch Group on
vegetal biology
Sani Marc Group4
Sanitationdivision
Food and beverage
division
pool/spadivision
Sani Marc Lab5
• Team LeaderPatrick Marchand, PhD
• 6 Chemists• 2 Microbiologists• 6 Lab Technicians
• 2 chemists dedicated to technical support
• In house regulatory support
Total of 112 years of experience in cleaning and disinfection
Why forestextracts?
Large potential Medical Nutraceutic Cosmetic Assainissement
6Antioxidant
Antiinflammatory
Anticancer
Antimicrobial
Acide caféique
Naringénine
Acide bétulinique
Kaempférol
Quercétine
LupéolCatéchine
Stigmastérol
Coumarin
Rhamnetin
Sani Marc interest
Disinfection represent more than half our sales in chemical
Needs for Greener alternative
Environmental awareness
Circular economy
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Why bark extracts ?
Forest resource in Qc
Need for diversification of forest products and getother high value co-products to support the industry
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http://www.rcinet.ca/fr/2015/11/25/quelle-sante-pour-la-foret-quebecoise-apres-des-annees-de-crise/
http://www.europages.fr/entreprises/sciage%20du%20bois.html
https://www.papnews.com/new-record-high-globally-traded-wood-chips-2016/
How to obtain a commercial antimicrobial extract from wood bark ?
2 industries = 2 realities
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Final extract user industry
Primary resource user industry
ResiduesQuality raw
material
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Harvested in the forest
SawmillPaper
Energy
Usine d’extractibles
Résidus d’extraction
Lumber
Raffinage selon besoins des marchés
How to obtain a commercial antimicrobial extract from wood bark ?
Setting up a value chain Forêt modèle du Lac Saint-Jean :
diversification of forest products to support the economy of the region
Implantation of a forest extract plant in Lac Saint-Jean
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Will of an economic development organization, motivated by the presence of an end user
Biomass conditionning for quality products
Secure biomass supply
Natural antimicrobial for sanitation products
Biomass supply
2015…
«Production of biocidal molecules from forest extracts for the manufacture of sanitation products»
12 Setting up a value chain2016…
Objective : develop a process for a future bark extracts plant in Lac St-Jean, to supply Sani Marc with natural
antimicrobial molecules
Methodology13
SupplyBark residus
caracterisation/preparation:Cleaning – sieving - grinding
Chemical caracterisation of bark residus
Process development
Crude extract screening for antimicrobial activity
Integration in Sani Marc formulation
Validation
250 000 green ton/year of bark (spruce, fir, birch, aspen)
Infrastructure for biomass conditionning(cleaning, sieving, grinding)
Heat
14 Results
SupplySupply
Cogénération St-Félicien
Bark residuscaracterisation/preparation : Cleaning - Sieving - Grinding
Bark residuscaracterisation/preparation : Cleaning - Sieving - Grinding
Fraction over 7mm for optimal yield
2017…
More than 1200 molecules identified
Alkaloids, phenolics, terpens
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Chemical caracterisationof bark residus
Chemical caracterisationof bark residus
0
5
10
15
20
25
30
% (w
/w)
Yield
Bouleau Peuplier Épinette Sapin
Results
Extracts biocidal against 6 microorganisms tested in lab, at 0,44%
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Extracts developmentwith antimicrobial activity
Extracts developmentwith antimicrobial activity
Results
Antibacterial testing method
Adapted from AOAC 960.09 Initial count 1010 bacteria Dissolve 4.5% crude extract in solvent Centrifugation to keep dissolved product only (3%) React with bacteria (1 hours) Neutralize disinfectant product (tween, buffer, thiosulfate,
lecithine) Plate Count 24h and 48 h later To be accepted by health canada we need 6 Logs
reduction
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Integration in Sani Marc formulation
Integration in Sani Marc formulation
Antibacterial efficiency Trial DMSO/water (30%/70%)
Inactivation of QUAT efficiency on E. coli (50 ppm; 10 minutes) 6 log reduction with water
1.5 log reduction with DMSO/water
No efficiency with fir extract in 3% on E. coli 4h, 21°C
Trial in MeOH/water (20%/80%) 4,6 log reduction on E. coli with 3% of fir extract
1h, 21°C
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0
2
4
6
DMSO MeOH
Log
CFU
/ml
Efficiency depends on solvent
Quat Fir
Formulation
Hard for now Crude extract not compltetly solubles (around 60%
soluble). Need to add Solvents
Surfactants
Dark brown solution
Important to purify Easiest to formulate
Diminution of concentration for bactericidal efficiency
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Reactive extrusion for increase the yied
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Process developmentProcess development
Pulp and paper technologies for extraction and separation
Results
21 Futur works Extracts separation and purification
Process development
Preliminary techno-economic analysis
More than one product…By focusing on a primary extraction plant to produce crudeextract, we make available a large diversity of molecules whichcould then be purified for specific markets (chemical, pharma, cosmeceutic,nutraceutic, etc.)
Industries
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Accelarated the research and ensure the technology transfert
Synergy
University-College-Industries Synergy
Acknowledgment