Supplementary Materials

Alginate dialdehyde meets nylon membrane: A versatile platform for facile and green fabrication of membrane adsorbers

M. Kamran Khan, a, b Jianquan Luo, a, b* Zhaoshuai Wang, a, b Rashid Khan, a, b Xiangrong Chen, a

Yinhua Wan a,b*

a State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of

Sciences, Beijing 100190, PR Chinab University of Chinese Academy of Sciences, Beijing 100049, PR China

*Corresponding authors: E-mail: jqluo@ipe.ac.cn (J. Luo); yhwan@ipe.ac.cn (Y. Wan)

Tel/Fax: +86-10-62650673

Alginate dialdehyde (ADA) Molecular weight

Molecular Weight of ADA was determined by using HPLC-GEC. A 30 cm x 0.75 cm TSK-G4000

PW column (Toyo Soda, Tokyo, Japan) preceded by a 2- µm filter (Rheodyne, CA) was equipped

with a Hitachi pump (Model L-6000), a precision injection valve (Rheodyne, 50 µL sample

loading) and a differential refractometer (R401 Waters Associates, France) connected to a

computer for sample detection. Dextran standards and glucose were injected at a

concentration of 1 mg/mL to establish the selectivity curve of the column. Injection volume was

40 µL for all analyses.

The calculated weight of the ADA was 150-200 KDa.

Membrane characterization by ATR-FTIR

The FTIR spectrum of pristine nylon, ADA-nylon, S-ADA-nylon (CEX), Me-affinity, His-affinity and

Pep-affinity are shown in Fig. S1. The peak at 1529 cm-1 corresponds to amides while the peak

at 1625 cm-1 shows carboxyl and carbonyl groups. Successful ADA coating was confirmed by the

appearance of new aldehyde and hydroxyl peaks around 1725 cm-1 and 3400 cm-1 respectively,

along with an increase in the carboxyl peak for ADA-nylon membrane. The disappearance of

aldehyde peak in the remaining scans confirms the successful immobilization of Sulphonic acid,

Electronic Supplementary Material (ESI) for Journal of Materials Chemistry B.This journal is © The Royal Society of Chemistry 2018

Histidine, Peptide and Me-affinity ligands via a Schiff’s base reaction. In the scan for S-ADA-

nylon membrane, the two new peaks for sulphonic groups at 1150 cm-1 and 1346 cm-1

confirmed the successful fabrication of S-ADA-nylon adsorber. The appearance of a broad peak

in Me-affinity nylon scan around 1180 cm- is for the chelated carboxylic groups. Successful

immobilization of histidine and peptide was confirmed by the increase in NH peak due to the

presence of NH groups in histidine and peptide.

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Chelated Carboxylic

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Pep-affinity adsorber N-HC=O

O-H

His-affinity adsorber

Figure S1. ATR-FTIR spectrum for pristine nylon, ADA-nylon, S-ADA nylon (CEX), Me-Affinity, His-

affinity and Pep-affinity adsorbers.

Figure S2. SEM Surface images of pristine nylon (a), CEX (b), Me-affinity (c), His-affinity (d) and

Pep-affinity (e) adsorbers.

Figure S3(a) . EDS spectra of pristine nylon membrane

Surface Scan

Figure S3 (b). SEM/EDS elemental mapping analysis (5000 x) of pristine nylon membrane, including surface topography, and individual elemental distribution map of C, O and N.

Figure S4 (a). EDS Spectra of ADA-Nylon membrane

Surface

Figure S4 (b). SEM/EDS elemental mapping analysis (5000 x) of ADA-Nylon membrane, including surface topography, and individual elemental distribution map of C, O and N.

Figure S5 (a). EDS Spectra of CEX membrane adsorber

Surface

Figure S5 (b). SEM/EDS elemental mapping analysis (5000 x) of CEX membrane adsorber, including surface topography, and individual elemental distribution map of C, O, N and S.

Figure S6 (a). EDS Spectra of Me-affinity membrane adsorber

ScanSurface Scan

Figure S6 (b). SEM/EDS elemental mapping analysis (5000 x) of Me-affinity membrane adsorber, including surface topography, and individual elemental distribution map of C, O, N and Cu.

Figure S7 (a). EDS Spectra of His-affinity membrane adsorber

Surface Scan

Figure S7 (b). SEM/EDS elemental mapping analysis (5000 x) of His-affinity membrane adsorber, including surface topography, and individual elemental distribution map of C, O and N.

Figure S8 (a). EDS Spectra of Pep-affinity membrane adsorber

ScanSurface Scan

Figure S8 (b). SEM/EDS elemental mapping analysis (5000 x) of Pep-affinity membrane adsorber, including surface topography (a), and individual elemental distribution map of C, O and N.

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Figure S9. High performance gel filtration chromatography results of feed, flow-through and

elution fractions obtained by pristine nylon membrane.

Figure S10. AKTA binding and elution chromatograms for the purification of IgG from IgG/HSA mixture by CEX (a), Me-affinity (b), His-affinity (c) and Pep-affinity (d) membrane adsorbers.

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Figure S11. AKTA chromatogram for IgG purification from Human serum by CEX (a) and Pep-affinity (b) adsorbers. The shoulder in the elution peak was due to the absorbance by KSCN.