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1 Factors to consider when ripening avocado Mary Lu Arpaia Univ. of CA Riverside, CA [email protected] Limitations to avocado postharvest handling v Time after harvest (fruit age) v Stage of ripeness – more difficult to handle “ripe” fruit v Mismanagement at any point in the handling chain v Fruit maturity 22 24 26 28 30 32 34 36 38 40 42 44 0 10 20 30 40 50 60 70 80 90 100 % Unsound fruit (5% threshold) Age when ripe (days) 2000 2001 2002 y = 0 + 0.02762*exp(days/5.203) r 2 = 0.82, n= 50, p<0.001 Dixon, Pak and Cutting Time after harvest influences the percentage of unsound fruit What we know about the avocado and why it responds to ethylene A climacteric fruit showing an increase in respiration and ethylene production during ripening Influenced by maturity, time after harvest, temperature and atmosphere 0 1 2 3 4 5 6 Days at 68F 0 50 100 150 0 100 200 300 400 Carbon Dioxide Ethylene ml CO 2 /kg/hr ul C 2 H 4 /kg/hr Adapted from Eaks (1978) for ‘Hass’
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Page 1: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

1

Factors to consider when ripening avocado

Mary Lu ArpaiaUniv. of CARiverside, CA

[email protected]

Limitations to avocado postharvest handling

v Time after harvest (fruit age)v Stage of ripeness – more difficult

to handle “ripe” fruitv Mismanagement at any point in the

handling chainv Fruit maturity

22 24 26 28 30 32 34 36 38 40 42 440

10

20

30

40

50

60

70

80

90

100

% U

nsou

nd fr

uit (

5% th

resh

old)

Age when ripe (days)

2000 2001 2002

y = 0 + 0.02762*exp(days/5.203)r2 = 0.82, n= 50, p<0.001

Dixon, Pak and Cutting

Time after harvest influences the percentage of unsound fruit

What we know about the avocado and why it responds to ethylene

• A climacteric fruit showing an increase in respiration and ethylene production during ripening

• Influenced by maturity, time after harvest, temperature and atmosphere

0 1 2 3 4 5 6

Days at 68F

0

50

100

150

0

100

200

300

400Carbon DioxideEthylene

ml C

O2/

kg/h

r

ul C2 H

4 /kg/hr

Adapted fromEaks (1978) for ‘Hass’

Page 2: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

2

External Chilling Injury Internal Chilling Injury

Susceptibility to low storage temperatures Body Rot

Stem End Rot

PostharvestDiseases

Relationship between dry matter (maturity) and final peel color

Final Peel Color = 3.06261- 0.00264DW+0.0020DW2

where DW = Dry weightR2 = 0.621 ***

1.0

1.5

2.0

2.5

3.0

3.5

4.0

4.5

5.0

5.5

6.0

10.0 22.8

Peel

Col

or

Dry Matter (%)

Actual Final Color

Predicted Final Color

Why Ripen Avocados?

Increase UniformityDecrease Checkerboarding

Untreated, fruit ripening may range from a few days to even weeks within a carton

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3

Maturity and “days to ripe”

Eaks, 1980, JASHS (Harvest August - June)

0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17

Oil Content (%)

4

6

8

10

12

14

16

18

20Days to Soft

1000 ppm propyleneno yes

Ethylene hastens ripening regardless of stage of maturity

Average Days to Eating Ripeness (<1.5 lbf) in response to 24 hour treatment of 40 ppm ethylene.

1/25/00 3/7/00 4/18/00 6/1/00 7/11/00 8/22/00

Harvest Date

0

5

10

15Days

EthyleneNO YES

RAIN

HassSeasonality/Quality

EarlySeason MidSeason LateSeasonEthylenetreatmentishighlyrecommended(checkerboarding)

Respondswelltoethylenetreatment

Littleornoethyleneneeded

Fruit moreproneto:• Shrivelingwhenripe• Decay• Internaldisorders• Mostsusceptibletolow

temperature

Bestfruitquality:• Noshriveling• Little decayexcept

whenharvestedafterrain

• Mosttolerantoflongtermstorage

Fruit moreproneto:• Decay• Internaldisorders• Difficulttostorelong

term• Unevenripening• Germinatedseed

Afterripefruitmaybe:• Notfullycolored• Waterytexture• Blandorgrassyflavor

Afterripefruitwillbe:• Green-blacktoblackin

color• Creamytexture• Goodtoexcellentflavor

Afterripefruitmaybe:• Blackincolor• DifficulttoPeel• Creamytodrytexture• Fulltorancidflavor

Time after harvest

• Ethylene has maximum benefit within 1-2 weeks of harvest

• Imported fruit if conventional shipment will need less time (24 hours or less)

• Imported fruit if CA shipped or 1-MCP treated may need longer treatment times

Time after harvest

California ‘Hass’; average of 3 harvests; 3 grower lots per harvest, 2006

0

5

10

15

0 days 7 days 14 days 28 days

Day

s to

< 1

.5 lb

f

Days at 5C

0 hr

24 hr

48 hr

Time after harvest decreases the impact of ethylene

Page 4: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

4

Even within lots of fruit there is variability in ripening – a way to control this is sorting by degree of ripeness into different categories

0 0g 3 3g 0 0g 3 3g 0 0g 3 3g 0 0g 3 3g 0 0g 3 3g 0 0g 3 3g0

5

10

15

20

25

Suggested treatment times for California ‘Hass’ avocados

• Early season fruit (November –February) 36 – 72 hours

• Mid-season fruit (March – June) 24 – 36 hours

• Late season fruit (July – October) 8 - 24 hours +/- ethylene

Management Issues

TemperatureVentilation/Air exchanges

üCareful MonitoringüPrompt Movement of fruit üWhat is the proper stage of ripeness?üWhere do you ripen the fruit?

Factors under your control

Educate yourself about the potential differences between varying sources of fruit – there are differences

• Pre-ripening inspection• Ripening management• Postripening management

Page 5: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

5

Ripening Management

• Uniform heating and cooling is ABSOLUTELY ESSENTIAL• Refrigeration needs to control the heat (6000 BTU per

pallet)• Forced air ripening is critical (1000 cfm/pallet) • Venting (preferably flow through, keep CO2 below 1%)• Source of Ethylene – as low as possible; physiologically you

only need ~10 ppm but practically use 60 - 100ppm• Fruit needs to be easily accessible in ripening room for

monitoring; especially if fruit is of varying arrival condition or multiple lots of fruit

• Keep good records

Can I use a banana ripening

room for avocado ripening?

Some considerations

0

50

100

150

200

250

300

350

Banana at 14C Banana at 18C Avocado at 20C

0

10000

20000

30000

40000

50000

60000

70000

Banana at 14C Banana at 18C Avocado at 20C

Peak Respiration (mg/kg-hr)

Refrigeration Load(BTU/T-day)

Can I use a banana room….. With some practical modifications

Refrigeration during ripening and cooling of fruit likely to be insufficient in a banana room

– Hot spots within pallet– Uneven ripening

Do not to overload the room

Page 6: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

6

Ethylene dose considerations

• Ethylene concentration– >20 ppm; no more than 100 ppm

• Fruit Maturity– Less mature; longer treatment

• Time after Harvest– With increasing time after harvest;

shorter durations needed

How much to apply?

Short exposures to ethylene can trigger ripening; threshold is believed to be around 10 ppm

Commercial application of 60 -100 ppm is recommended

Source: I. L. Eaks, UC, Riverside

10, 100 ppm

1 ppm

0 ppm

Temperature Management

• Avocados have a VERY high rate of respiration during ripening = HEAT

• Efficient warming/cooling of fruit essential

• Airflow essential to maintain proper pulp temperature (20C)

Impact of high temperatures– Delayed/uneven ripening– Increased decay

The impact of Temperature (24 or 48 hours) on ripening performance of ‘Hass’ avocado

0

2

4

6

8

10

12

14

16

20 25 30 35

Daysto

Ripe

Temperature(C)during24or48hrHolding

05101520253035

20 25 30 35

Stem

End

Rot(%

)

Temperature(C)during24or48hrHolding

0

5

10

15

20

25

20 25 30 35

Body

Rot(%

)

Temperature(C)during24or48hrHolding

dc

ba

cbc

aba

b

bab

a

§ High temperatures are DETRIMENTAL§ The outcome is delayed or inhibited

ripening and increased decay§ Keep temperatures below 21 C

No significant difference due to duration

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7

Ripening temperature influences final peel colorCox et al, 2004, PH Biol. Tech.

‘Hass’ AvocadoRipening Hass at different temperatures

6567697173757779818385

0123456789

15⁰C 18⁰C 20⁰C 23⁰C 25⁰C

HueAn

gle

Daysto

Ripe

RipeningTemperature

DaystoRipeHueangle

Ripening ManagementWhen do you turn off the gas?

• You don’t need the gas until ripe; a short duration treatment will “trigger” ripening

• Fruit may soften but may not color – maturity and other factors involved

• The best way to gauge the rate of softening is with a penetrometer…not your fingertips or buttons “popping”

• Fruit maturity is an important variable

The penetrometer is a tool to judge the relative stage of ripeness

How long can I hold ripe or partially ripe fruit?

• Fruit continue to soften in storage• Partially ripened fruit held at 50F

(10C) can develop pink staining especially if held longer than 1 week

• Fruit can be successfully held at 34F (1C) for up to a week

• Is there a difference in eating quality?

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8

Managing Ripe Fruit• Decay increases with increasing ripeness;

accelerates in “overripe” fruit• Don’t hold fruit for long periods of time that are

partially ripe – increased chilling injury• Bruising increases with advancing ripeness –

Protect fruit• Peel color at “slicing” or “guacamole” ripe does

not necessarily mean the fruit needs to be completely black!

These are issues wherever fruit are ripened

The outcome of “ripe” fruit

Ripe fruit at retail level has greatly increased consumption,

HOWEVER…..• Greater challenge in

temperature management

• Fruit sensitivity to damage greatly enhanced

Considerations for successful avocado ripening

• Temperature management is CRITICAL– Too high; ripening

inhibited and increased decay

– Too low; ripening is slowed and lose benefit

• Fruit Maturity– More mature; less time

• Time after Harvest– After storage; less time

• Avoids delays in marketing• Minimize fruit handling

Checklist

Know the history of the fruit

Quality; don’t use stressed fruit

Standardize fruit size and maturity

Uniform warming and cooling

Careful monitoring; don’t overripen

CONSUMER/MARKET Education

Additional information

• Contact [email protected]

• UC Davis Postharvest Center websitehttp://postharvest.ucdavis.edu/

• California Avocado Commission websitehttp://www.californiaavocado.com/ripening-and-handling/

• General information on avocadoswww.avocadosource.com

Page 9: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

9

HassSeasonality/Quality

EarlySeason MidSeason LateSeasonEthylenetreatmentishighlyrecommended(checkerboarding)

Respondswelltoethylenetreatment

Littleornoethyleneneeded

Fruit moreproneto:• Shrivelingwhenripe• Decay• Internaldisorders• Mostsusceptibletolow

temperature

Bestfruitquality:• Noshriveling• Little decayexcept

whenharvestedafterrain

• Mosttolerantoflongtermstorage

Fruit moreproneto:• Decay• Internaldisorders• Difficulttostorelong

term• Unevenripening• Germinatedseed

Afterripefruitmaybe:• Notfullycolored• Waterytexture• Blandorgrassyflavor

Afterripefruitwillbe:• Green-blacktoblackin

color• Creamytexture• Goodtoexcellentflavor

Afterripefruitmaybe:• Blackincolor• DifficulttoPeel• Creamytodrytexture• Fulltorancidflavor

Back to the consumer - FlavorRelationship between oil and acceptabilityp Lee et al, 1983, J. Amer.

Soc. Hort Sci.p Minimum acceptable taste

score = 7p Demonstrated close

relationship between oil and dry matter

p HASS varietyn Oil content = 11.2%n Dry weight equivalent =

22.8%Taste and oil development during maturation of 'Fuerte'fruit grown at Irvine.

Sensory panel analysis

• Texture• Flavor• Overall acceptability

Avocado texture and acceptability

Perc

ent r

espo

nse

Page 10: Factors to consider when Limitations to avocado ripening ...postharvest.ucdavis.edu/files/261292.pdf · Avocado flavor and acceptability Sweetalso associated with higher hedonic scores

10

Avocado flavor and acceptability

Sweet also associated with higher hedonic scores but at lower levels of response (30%)

Avocado season

Harvests taken throughout the season

Jan Sept

Analyzed samples from each harvest for sensory and quality attributesas well as aroma volatile content

What is the effect of maturity on flavor and what drives these flavor changes?

All samples were ripened to a similar firmness

Changes in key sensory attributes and “grassy” volatilecontent associated with increasing maturity

• Avocados were more liked with increasing maturity• This was associated with increased richness and creaminess and decreased grassiness• The decrease in grassiness paralleled a decline in volatiles with grassy aromas

Summary1. Avocados that were most liked by our sensory panelists were

described as having a creamy, smooth, buttery texture with richness, nuttiness and a minimum of grassy flavor.

2. Avocados contain aroma volatiles that likely help determine the flavor

3. Aldehydes, such as hexanal, were clearly associated with grassy flavor

4. Ripening led to a decline in soluble carbohydrates and in a number of aroma volatiles, hexanal being the most prominent


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