To find out more about how semiconductor sequencing technology works and to hear what scientist are doing with the Ion PGM™ sequencer, join the Ion Community at: http://ioncommunity.iontorrent.com

Register for the Life Grand Challenges for your chance to win $1 million, at:www.lifetechnologies.com/grandchallenges

fastest next-gen workflow10X more throughputfastest-selling sequencerall in six monthsIon Personal Genome Machine™ Sequencer

*The content provided herein may relate to products that have not been officially released and is subject to change without notice.

FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE. © 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc.

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Ion 316™ sequencing chiptenfold increase in throughput after just six months

Semiconductor Sequencing

Semiconductor technology has transformed every industry it has touched, from computing to photography to music. And now it’s transforming the life sciences. The Ion Personal Genome Machine™ sequencer is powered by an innovative semiconductor chip which makes it faster, easier to use, and more accessible than any light-based solution. Experience the speed, simplicity, and scalability of semi- conductor sequencing with the Ion PGM™ sequencer.

Ion Torrent has pioneered an entirely new approach to sequencing. Instead of using light as an intermediary to sequence DNA, Ion Torrent uses semiconductor technology and simple chemistry. Ion Torrent translates chemical signals into digital informa-tion, much as a CMOS chip translates photons into pixels.

Ion semiconductor sequencing brings the entire design, fabrication and supply chain infrastructure of the semiconductor industry—a $1 trillion investment—to bear on the challenge of sequencing DNA. Just as the microprocessor enabled desktop computing to displace the mainframe, Ion Torrent semiconductor technology will inevitably democratize sequencing, putting it within the reach of any lab.

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“ The biggest advantage [of the PGM] from my point of view as a public health official is that it’s speedy, and speed is what is needed at the moment.”PROF. DR. MED. DAG HARMSEN UNIVERSITY HOSPITAL, MÜNSTER, GERMANY

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Only minutes of hands-on time 2 H

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Under one hour for short reads

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WORKFLOW TIMES >

fastest next-gen workflow

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01Construct Library

The new Ion Xpress™ Fragment Library Kit offers a novel enzymatic fragmentation process that has reduced library preparation to 3.5 hours, 60 percent faster than six months ago.

+ ION K I T S + ION ONE-T OUCH ™ S Y S T EM + ION P GM ™ SEQUENCER + T OR R EN T SER V ER

Ion Torrent’s single-day workflow is now the fastest in next-generation sequencing, at under eight hours. The time was reduced approximately threefold in just six months, thanks to key breakthroughs at each stage of the workflow:

02Prepare Template

The Ion OneTouch™ System* for template preparation, shipping in the third quarter, has added a component to automate enrichment, the Ion OneTouch™ ES.* Now the entire template preparation process is fully automated and can be completed in three hours, 70 percent faster than six months ago.

03Run Sequence

The new Ion 316™ chip has a two-hour run time for 100 base pair reads, just like the Ion 314™ chip. Run times are reduced to less than one hour when sequencing short reads.

04Analyze Data

The new Torrent Suite 1.4 analysis software cuts analysis time to just 30 minutes on the Ion 314™ chip and one hour on the Ion 316™ chip, while also delivering a significant improve-ment in base-call accuracy.

“ [The PGM] takes the shortest time to generate genomic data.” JUNJIE QIN BGI, CHINA

nothing gets better faster than Ion semiconductor sequencing

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Six months to 10-fold improvement

In the six months since the Ion PGM™ sequencer launched, its throughput has increased tenfold, its workflow has become the fastest in the industry, and it’s now the fastest-selling sequencer in the world, with placements in more than 40 countries. In the second half of the year you’ll see more of the same. Read lengths will double to 200 base pairs and throughput will increase by tenfold—again.

All of these advances are a direct result of Ion’s semiconductor sequencing technology and native chemistry, which make sequencing faster, simpler, and more scalable than ever before.

More Throughput, Better Value

The scalability of Ion semiconductor sequencing will enable a hundredfold increase in throughput this year. The new Ion 316™ chip has 100 Mb of throughput, tenfold more than the original Ion 314™ chip. The Ion 318™ chip, available in the fourth quarter, will deliver 1 Gb of throughput. Each of the three chips will work on the Ion PGM™ sequencer, giving users the flexibility to buy only as much throughput as they need for an experiment, from 10 Mb to 1 Gb.

Ion semiconductor sequencing offers exceptional value as well as perfor-mance. The Ion 314™ chip is now available for just $99 USD, which enables the sequencing community to develop novel methods and applica-tions. Ion Torrent is able to lower the price because of the significant sales volume generated by the Ion 314™ chip and the economies of scale inherent in semiconductor manufacturing.

Better Accuracy

The Ion PGM™ system’s accuracy has improved by an order of magnitude since it was launched six months ago, as a result of the system’s speed and simplicity. Ion Torrent has capitalized on the inherent accuracy of its simple sequencing chemistry, achieving more signal, better signal processing and improved base calling. Accuracy improved quickly in part because the Ion PGM™ sequencer’s two-hour run times enable Ion Torrent R&D teams to quickly test and refine ideas. Improve-ments in accuracy will help Ion Torrent reach 200 base pair reads in the second half of this year and 400 base pair reads in 2012, making many new applications possible for researchers.

More New Products

Additional new products now available include an early-access version of the Ion DNA Barcoding 1-16 Kit* for multiplexed sequence analysis, the Ion Library Quantitation Kit for TaqMan®-based quantitation of Ion DNA libraries, and the Ion Sphere™ Quality Control Kit for template bead quality control.

The Ion PGM™ Sequencer in Customers’ Hands

Scientists turned to the Ion PGM™

sequencer to identify a deadly strain of E. coli in Europe in June because the system’s two-hour run time was faster than anything else on the market. Scientists at BGI sequenced the bacterium within three days, and a team at the University of Münster sequenced the strain and developed a field-deployable test in just five days.

Scientists around the world also have reported exceptional throughput results with their first runs on the Ion 316™ chip. Early-access users at Baylor College of Medicine recently broke Ion Torrent’s internal record for highest output, after reporting 287 Mb from a single Ion 316™ chip run, and the Broad Institute reported up to 250 Mb. Most recently, the Wellcome Trust Sanger Institute reported that one of the first runs of an Ion 316™ chip on their Ion PGM™ sequencer produced a total yield of 194 Mb.

ION SEMICONDUCTOR SEQUENCING RAPIDLY IMPROVING ACCURACY

† www.lifetechnologies.com/ion314data

REMARKABLE SCALABILITY: 10MB TO 1GB IN ONE YEAR (100X)

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10 Mb 100 bp

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316JUL:2011

100 Mb 100 bp

318*Q4:2011

1 Gb 200 bp

To find out more about how semiconductor sequencing technology works and to hear what scientist are doing with the Ion PGM™ sequencer, join the Ion Community at: http://ioncommunity.iontorrent.com

Register for the Life Grand Challenges for your chance to win $1 million, at:www.lifetechnologies.com/grandchallenges

fastest next-gen workflow10X more throughputfastest-selling sequencerall in six monthsIon Personal Genome Machine™ Sequencer

*The content provided herein may relate to products that have not been officially released and is subject to change without notice.

FOR RESEARCH USE ONLY. NOT INTENDED FOR ANY ANIMAL OR HUMAN THERAPEUTIC OR DIAGNOSTIC USE. © 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners. TaqMan is a registered trademark of Roche Molecular Systems, Inc.

CO31580 0711