Fiber photometryEllen Koch

Data Binge

November 30, 2018

Fiber photometry

• Measure neural activity such as GCAMP of

a cell population in a brain area of interest

• Can access deep brain structures

• Freely moving animals

• Chronic preparation

Nature Protocols 2014

Cell 2014

Nature Letters 2013

Tucker

Davis

Technologies

Data analysisRaw signals

Control fitted to Signal

Control subtracted from Signal,

then normalized to calculate %dF/F

Data analysisRaw signals

Control fitted to Signal

Control subtracted from Signal,

then normalized to calculate %dF/F

Data analysisRaw signals

Control fitted to Signal

Control subtracted from Signal,

then normalized to calculate %dF/F

Experimental Plan

2+ weeks 1+ weeks

Virus Injection Fiber implant Slice experiments

and fiber placement

Photometry

experiments

AAV9-Syn-GCAMP7f

Surgery

Surgery

GCAMP injected mice - Open Field• Activity in striatum correlated with movement of the mouse in an open field

• Boxes = mouse not moving

GCAMP injected mice – Pick up

GCAMP injected mice - Rotarod

D2-GCAMP: Open Field

D2-GCAMP: Rotarod

Thy1 GCAMP: Prefrontal cortex

Pick up Rotarod

PFC: froot loop

Analysis: Mean dF/F

GCAMP activation and rotarod motor learning• Average GCAMP activity reduces as mouse’s performance improves

Accelerating Rotarod

Analysis: Peak frequency and amplitude

Z Scores

Multi Fiber PhotometryKim et al. 2016

Sych et al. 2018

Thanks for listening!