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GROUP 3

Mazlina Ismail

Wahurun Iin Mohd Taha

Hayati Atiqah Salleh

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Boesenbergia rotunda (L.) Mansf. Kulturpfl. known as kra-chai & temu kunci.

Characteristics: small herbaceous plant

short, fleshy or slight rhizomes with a few leaves. unique scent and also slightly sharp taste.

(Amy Yap Li Ching et al., 2007).

Scientific Classification

Kingdom : PlantaeOrder : Zingiberales

Family : Zingiberaceae

Genus : Boesenbergia

Species : B. rotunda

INTRODUCTION

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Oftenly used as food ingredient and in folkmedicine treatment

Rhizome (enzymes) as tonics & lotionIngredients

Commercially use in pharmaceuticals products

Pharmacological importance - presence offlavanoids, essential oil and chalcones

(pinostrobin, pinocembrin, cardamonin,alpinetin and boesenbergin molecules)

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Primary health care project of Thailand- rhizome

part to treat dyspepsia

Potential values (biological activities)

antipyretic, analgesic, anti-mutagenic, anti-inflammatory, antioxidant enzymes, anti-humanimmunodeficiency virus 1 (anti-HIV-1) proteaseinhibition, anti-tumour, antibacterial, antifungal,

antispasmodic, and insecticidal activities.

Enhanced the acceleration of wound healing

area

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a) To observe the effect of plant medium supplemented

with different level hormones concentration on

growth ofB. rotunda in vitro culture.

b) To determine the best level different hormones and

hormone combinations on growth ofB. rotunda in

vitro culture.

OBJECTIVES

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Freq of shoot formation in the

explants is different for

different MS salt strength

Supplementation of the PGR -

BAP and NAA (BN) is better for

shoot regeneration in BR

HYPOTHESIS

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MATERIALS

B. rotunda

Use B.rotundas stem

(explants) obtained from in

vitro culture

Hormones

1. Benzyl aminopurine (BAP)

2. 1-naphtalenacetic acid (NAA).3. Indole acetic acid (IAA)

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TREATMENTS

I MSO

II

III

IV

V

VI

MS + BAPa) 0.5mg/L

b)1.0mg/L

c) 2.0mg/L3/4MS

3/4MS + BAPa) 0.5mg/L

b)1.0mg/L

c) 2.0mg/L3/4MS + BN

3/4MS + BI

NAA 0.1mg/L 0.5mg/L

BAP

0.5mg/L a b

2.0mg/L c d

IAA 0.1mg/L 0.5mg/L

BAP

0.5mg/L a b

2.0mg/L c d

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RESULT

MS basalsupplemented

with:

(a)0.5mg/L BAP

(b)1.0 mg/L BAP

(c) 2.0 mg/L BAP

Media

Response Frequency(%)

week 2 week 4I 16.67% 16.67%

II 0.00% 16.67%

III (a) 22.22% 33.33%

III (b) 0.00% 0.00%III (c) 0.00% 0.00%

IV (a) 83.33% 83.33%

IV (b) 50.00% 50.00%

IV (c) 33.33% 66.66%

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MS supp with

+ 0.1mg/L NAA

+ 2.0 mg/L BAP

MS supp with

+ 0.5mg/L IAA

+ 2.0 mg/L BAP

Media

Response freq (%)

week 2 week 4

I 16.67% 16.67%II 0.00% 16.67%

III (a) 22.22% 33.33%

III (b) 0.00% 0.00%

III (c) 0.00% 0.00%

IV (a) 83.33% 83.33%IV (b) 50.00% 50.00%

IV (c) 33.33% 66.66%

V (a) 0.00% 33.33%

V (b) 0.00% 50.00%

V (c) 50.00% 100.00%

V (d) 25.00% 58.30%

VI (a) 0.00% 0.00%

VI (b) 0.00% N/A

VI (c) 66.67% 66.67%VI (d) 100.00% N/A

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Media

% of

adventitious

shoot

generation

I 11.22II 5.61

III (a) 5.61

III (b) N/A

III N/A

IV (a) 6.73

IV (b) 5.61

IV 8.42

V (a) 5.61

V (b) 5.61V 11.22

V (d) 7.22

VI (a) N/A

VI (b) N/A

VI 8.42

MEDIA VI (d) - Shoots formation on MSsupplemented with 2mg/L BAP + 0.5 mg/L IAA

MEDIA V (c) - Shoots formation on MS

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MS

higher shoot production rate agreement with other monocots (K.galanga, Trillium

erectum and T.grandiflorum) (Shirin et al, 2000) (Pence & Sourkp, 1993)

Combination of both BAP+NAA & BAP+IAA

showed multiple shoot formation Previous study by (Geetha, 1993) support this however study

by (Swapna, 2004) did not show the same reult

Similarity between the highest shootformation frequency Media V (c) & VI (d): [BAP] 2.0mg/L

DISCUSSION

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CON

MS produced higher percentage ofmultiple shoot formation than full strength

MS media

Supplementation of 2.0mg/L BAP + auxinworks in multiple shoot formation

Media V (c), V (d), VI (c) and VI (d) showed

higher number of shoot formationThe best media is MS supplemented with

2.0mg/L BAP + auxin

CONCLUSION