Fish Reproductio

n

Spawning?Spawning?

Egg MaturationOvulationSpawning

TemperaturePhotoperiod

Spawning?Spawning?

TemperatureTemperature

Pineal Gland

Photoperiod

Opening for pineal glandOpening for pineal glandOpening for pineal glandOpening for pineal gland

Weather Weather

SubstrateSubstrate

Mate Availability&

Pheromones

Mate Availability&

Pheromones

TempTempPhotoperiodPhotoperiod Mate AvailabilityMate Availability

SpawningSubstrateAvailability

SpawningSubstrateAvailability

HealthStatus

WeatherWeather

PheromonesPheromones

Goldfish Spawning

• Feed broodfish • Move to warm holding tanks• Use lights to lengthen photoperiod• Move to shallow spawning tanks• Add spawning mats• Increase depth to cover mats• Use some old water

Brain to PituitaryGonadotropin

HormoneReleasing Hormone

GNRH

Pituitary

Gonadotropin

Pituitary Pituitary to Nadsto Nads

EstrogenTestosterone

Nads to Nads

Estrogen

Vitellogenin= YolkVitellogenin= Yolk

Ovary to liver

Testosterone

Nads to Elsewhere

Eye Pineal Others...

Brain

Pituitary

Testes

GnRH

Gonadotropins

Testosterone

Ovary

Estrogen

Sperm development, Secondary sexual characteristics, Spawning Behavior

ImmatureSperm

GlandularGlandularEpitheliumEpithelium

Swordfish sperm packets in various stages of development

Sperm Heads

Flagella

Gonopodium

Eye Pineal Others...

Brain

Pituitary

Testes

GnRH

Gonadotropins

Testosterone

Ovary

Estrogen

Secondary Sexual Characteristics, egg development, yolk production (liver), spawning behavior, ovulation

Yolk

Shell

Follicle

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

TempTempPhotoperiodPhotoperiod Mate AvailabilityMate Availability

SpawningSubstrateAvailability

SpawningSubstrateAvailability

HealthStatus

WeatherWeather

PheromonesPheromones

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

Leutenizing HormoneReleasing Hormone

LHRH

GNRH

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

DriedPituitary

Too many hormones !

Gonadotropin

Human ChorionicGonadotropin

HCG

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

Eye Pineal Others...

Brain

Pituitary

Testes

Liver

GnRH

Gonadotropins

Testosterone

Ovary

YolkProtein

Estrogen

Secondary Sexual Characteristics

Induced Spawning1. Temp, photoperiod, other cues2. LHRH

1. Replaces GnRH

3. Gonadotropin from dried pituitary1. Clumsy!

4. Human Chorionic Gonadotropin (HCG)

1. Replaces gonadotropin

5. Hormones only finish egg development and spawning

1. Temp, photoperiod, other cues2. LHRH

1. Replaces GnRH

3. Gonadotropin from dried pituitary1. Clumsy!

4. Human Chorionic Gonadotropin (HCG)

1. Replaces gonadotropin

5. Hormones only finish egg development and spawning