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Fitzpatrick Institute for PhotonicsDuke University
Tuan Vo-Dinh
Physical Facilities
Fitzpatrick Center for Interdisciplinary Engineering, Medicine, and Applied Science“FCIEMAS”
$100M, 300,000-sqft FacilitiesDedication: November, 2004
Fitzpatrick Institute for Photonics (FIP)120,000-sqft Facility
65 Faculty and Research Groups20 + Departments at Duke
Fitzpatrick Institute for PhotonicsDuke University
Maintain Excellence in Current Core Competencies
• Biophotonics
• Nano and Micro Systems
• Quantum Optics & Information Photonics
• Photonic Materials
• Advanced Photonic Systems
Strategic focus on target applications based on competencies
Develop New Competencies inSelected Target Areas
• Nanophotonics
• Systems Modeling & Theory and Data Treatment
• Novel Spectroscopies
Nanoprobe
Single Cell
Optical Coherence Tomography
Joseph Izatt
Clinical Systems at Duke Medical Center
Company Spin-Off: Optigen, Inc
Breast Biopsy Needle Nimmi Ramanujam
Fourier Domain Low Coherence Interferometry LCI (fLCI): Spectral Characterization of Nuclear
MorphologyAdam Wax
• Can determine longitudinal diameter of nucleus of cells in vitro• Comparison of fLCI with confocal microscopy shows good accuracy
Modality Mean Diameter
Standard Deviation
fLCI 6.9 µm 0.8 µm
Confocal Microscopy
6.8 µm 1.1 µm
R.N. Graf and A. Wax,Opt. Express 13, 4693 (2005).
DyeModule
NitrogenLaser
Poly-chromator
Multi-channelDetector
PC
Optics
Endoscope
BifurcatedOptical Fiber
Minimally Invasive In Vivo Cancer Diagnostics
Clinical Trials: Over 100 patients
98% Sensitivity; 95% Specificity
Halfshell Array as SERS Substrates
Support Nanoparticle Layer
Metal layer
Nanoparticle-based
Substrate Parameters:
• Nanoparticle material
(e.g. alumina, titanium
dioxide, polystyrene,
fumed silica)
• Nanoparticle size (e.g. 50
nm- 500 nm)
• Metal (e.g. silver, gold,
copper)
• Metal thickness (e.g. 50-
100 nm)
Scanning electron micrograph of silver-coated polystyrene microspheres
Surface-Enhanced Raman Scattering (SERS) Nanoparticle Probes
• Advantages of SERS-based labels– Comparable sensitivity to fluorescence– Resistance to photobleaching and
quenching– Enhanced spectral multiplexing (sharp lines
minimal overlap)
Raman Label
Metal Particle
Bioreceptor
• Targeting molecules to be used will include:– Specific bioreceptors
• Antibodies
• DNA constructs that are complementary to a mRNA target sequence
• Enzymes
0
40000
80000
120000
160000
Raman Shift (cm-1)
Ra
ma
n In
ten
sit
y (
a.u
.)
400 600 800 1000 1200 1400 1600
0
40000
80000
120000
160000
Raman Shift (cm-1)
Ra
ma
n In
ten
sit
y (
a.u
.)400 600 800 1000 1200 1400 1600
Nanosensor
Single Cell
Fig. 8
Nanosensor for Single-Cell Analysis
MitochondriaCaspase-7
Cytochrome cCaspase-9
Apap-1
Proca
spas
e-9
Apoptosome
The Biochip TechnologyThe Biochip Technology
• 2-D array of independently operating
photodiodes
• On-board signal amplification and data
treatment
• CMOS-based microelectronics
integrated onto a single platform
• Coupled to compact sampling system
ADVANTAGES:
• Compact design
• Low power consumption• Multiple assays possible on single platform
• Increased throughput • Cost effectiveness
• Microscale sampling capability
Fitzpatrick Institute for PhotonicsBridging the Gap: From the Nano World to Field Devices
Integrated Nano and Micro Systems
Traditional Approach High Cost, Low Volume, Niche Separate Component Packaging
Next Generation Low Cost, High Volume, Pervasive Integrated/Embedded OE Packaging Take OE packaging from discrete to integrated (emulating the IC revolution in the last 50 years) Making tabletop systems into ladybug size
Nan Marie Jokerst, FIP
Nanoparticle Plasmonics for Molecular DetectionA A Lazarides, Duke University
Objective: Dsign and demonstrate reconfigurable plasmonic assemblies for use as sensors in optoelectronic detection systems and in cells
Detection and Transduction: Thermodynamic principles of soft matter assembly
can be used to design self-assembling biomolecule-linked assemblies
Reconfigurable DNA nanostructures can be designed to control interparticle separation and coupling
Plasmonic sensors can be be integrated onto optoelectronic substrates or used as portable signallers in fluid biosamplese or cells
On/ off states of a chip fragment
BIomolecule-driven reconfiguration using DNA nanostructures
Microscopy SpectroscopySpectrum
predicted from structure
Single assembly spectroscopy with Jack Mock and David Smith
with T H LaBean
Self-Assembling DNA Nanostructures Thom LaBean, FIP, Duke University
500 x 500 nm
• Biomolecular self-assembly.
• DNA building blocks.
• Organization of other materials.
• Photonic applications.
• Future directions and applications.
Negative at Optical WavelengthsCloaking Materials
David Smith, Duke University
Properties of Plasmons:
•Surface modes
•Spatial variation of optical wavelengths on a scale <<.
•Large local field enhancements
•Large local density of states
•Contributes to SERS and SERRs phenomena
•Fast (fs) time scales
• Miniaturized systems containing:• Nanoprobes and microsensors• Control and signal processing electronics• Micro and nanofluidics• Wireless alarm/data transmission• Microactuators
• Integrate all of these components into a single mini-micropackage• Patch, Probe, Stamp-sized
• Operates on a coin battery• Continuous monitoring with pre-set alarm conditions and sensing
informationOR
• One shot sensing (disposable probes)• Wireless data download
Technologies for Integrated Nano- and Micro-Systems
BiophotonicsIzatt, Joseph, Program Director Brady, DavidJohnson, G. AllanProvenzale, JamesRamanujam, NimmiShang, AllanVo-Dinh, TuanWarren, Warren*Wax, Adam
Nano/Micro SystemsJokerst, Nan , Program DirectorBrooke, Martin Fair, Richard LaBean, ThomasMassoud, Hisham Tian, JingdongYoshie, Tomoyuki*
Quantum Optics and Information PhotonicsGauthier, Daniel, Program DirectorBaranger, Harold Kim, Jungsang Thomas, JohnWarren, Warren*
Photonic MaterialsSmith, David, Program DirectorBrown, AprilCummer, SteveGlass, Jeff Jokerst, Nan*Massoud, Hisham* Stiff-Roberts, Adrienne
Fitzpatrick Institute for PhotonicsTuan Vo-Dinh, Director
Advanced Photonic SystemsReichert, William, Program DirectorBrady, RachaelChakrabarty, KrishJohnson, KristinaEdwards, GlennGuenther, BobMassoud, Hisham* Ozev, Sule
NanophotonicsLeong, Kam, Program Director Chikolti, AsutoshLazarides, Anne Liu, JieSmith, David*Vo-Dinh, Tuan*Wax, Adam*Yoshie, Tomoyuki
Systems Modeling, Theory & Data TreatmentYang, Weitao, Program DirectorBeratan, David, Dwyer, ChrisKrolik, JeffreyLiu, QingPitsianis, NikosSun, XiaobaiVenakides, Stephanos
Novel SpectroscopiesWarren, Warren, Program DirectorBrady, DavidIzatt, Joseph*Palmer, RichardSimon, John Vo-Dinh, Tuan*Wax, Adam*
A Global Photonics VisionAn Initiative at the New Frontiers
of Science and Technology
Nanosystems & Nanophotonics
Biophotonics Information Photonics& Quantum Optics
NANO BIO INFO
OPTO