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Flag IP

Date post:	04-Jan-2016
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SA. SL. Flag IP. IgG purified. F3. -ve. Spt7. Gcn5. Ada2. Ubp8. Taf5. Spt3. Spt8. 4741. 250. SA. SA. SL. SL. F3. F3. 150. 1. 2. 3. 4. 5. 6. 7. 8. 9. A. B. spt7 D 0 SPT7. - + - +. Anti-myc Ab. TAP-Flag Spt7. 250 -. 160. 150 -. 1 2. - PowerPoint PPT Presentation

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1

Flag IP Spt7 -ve IgG purified Spt8 Gcn5 Ada2 Ubp8 Taf5 4741 250 SA Spt3 150 1 2 F3 8 3 4 5 6 9 7 SL SA SL F3 16 0 1 2 3 4 Anti-myc Ab A B 0 0.2 0.4 0.6 0.8 1 1.2 1.4 1.6 1.8 2 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 Fraction SAG A SLIK Form 3 670 kD a void 158 kD a Densometry C SA SL F3 TAP-Flag Spt7 - + - + 250- 150- spt70 SPT7 1 2 D

Transcript

Page 1: Flag IP

Flag IP

Spt7-ve

IgG purified

Spt8Gcn5 Ada2 Ubp8 Taf5 4741250

SA

Spt3

1501 2

F3

83 4 5 6 97

SL

SASLF3160

1 2 3 4

Anti-myc AbA B

0

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

1.8

2

15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31Fraction

SAGASLIKForm 3

670 kDavoid 158 kDa

Den

som

etry

C

SASLF3

TAP-Flag Spt7- + - +250-

150-

spt70 SPT7

1 2

D

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