PowerPoint-Prsentation

FLAG-PAWS1HA-SMAD1BMPGFP-SMAD4+-+++-------+++++++--++++--+++--IP: GFPIB: HAIB: FLAGIB: P-SMAD1 S206IB: GFPIB: P-SMAD1 S206IB: HAIB: FLAGIB: P-SMAD1IB: GFP

Input

Vogt et al. Figure S1A

IB: SMAD1InputIP: SMAD1-TGFBMPIgGD

+---+GFPGFP-SMAD1GFP-SMAD5GFP-SMAD8FLAG-PAWS1-+--+--+-+---++IB: PAWS1IB: PAWS1

IB: GFPIB: GFPIP-FLAGinputHEK293B

+----+FLAGFLAG-SMAD1FLAG-SMAD1 MH1FLAG-SMAD1 LinkerFLAG-SMAD1 MH2HA-PAWS1-+---+--+--+---+-+IB: HA-PAWS1IB: FLAGIP-FLAGinputHEK293IB: FLAGIB: HA-PAWS1----++CA

MDA-MB-231U2OSHEK 293RKOHaCaTHeLaMCF7PC3KBM7IB: PAWS1 lowIB: PAWS1 highIB: SMAD1IB: GAPDHB

IB: PAWS1 lowIB: PAWS1 highIB: SMAD1IB: GAPDHPAWS1PAWS1BrainHeartLungKidneyLiverSpleenThymusPancreasAdipose tissueMuscleTestisEpididymisOvariesVogt et al. Figure S232P-AutoradLDN-193189 (M)0.0000.0010.0030.0100.0300.1000.3001.0003.00010.00ALK2Coomassie

0.0000.0010.0030.0100.0300.1000.3001.0003.00010.00ALK6

Vogt et al. Figure S3Phospho-PAWS1Peak1Peak2BMPR1 + GST-PAWS1(S610A)Vogt et al. Figure S4ABCP1P2662.6184 +2P953.4414 +P

Vogt et al. Figure S5

IP: PAWS1; IB: PAWS1IB: P-SMAD1IB: SMAD1IB: GAPDHIP: PAWS1;IB: P-PAWS1 S610BMP for min.01545120240480

BxPC3-BMP 2BMP 4/70123ASNSNEDD9fold changeVogt et al. Figure S6ABP