ISTAISTAForest Tree and Shrub Seed Forest Tree and Shrub Seed
CommitteeCommittee20020077 -- 20020088
ISTA Annual Meeting, BolognaJune 17, 2008
Fabio Gorian and Zdenka Procházková
ItalyBeti Piotto
BrazilIsolde D. Kossmann FerrazMembers:
United StatesKari Fiedler
NorwayHeidi Røsok Bye
CanadaDale Simpson United StatesVictor Vankus
United KingdomMoctar Sacande
United KingdomHugh W. Pritchard
BrazilAntonio MedeirosGermanyStefanie KrämerCanadaDave Kolotelo United StatesRobert P. Karrfalt United KingdomPeter G. Gosling SlovakiaElena Foffova
ItalyFabio GorianVice-chair:Czech RepublicZdenka Prochazkova Chair:
1. Introduction of New MethodsA.A.Rules DevelopmentRules Development
2.Introduction of New Species
5^ FTS WORKSHOPJUNE 12-14, 2008 PERI , ITALY Local Organizers: F. Gorian + S. Pasquini
C. Workshops and seminarsC. Workshops and seminars
GOALTo compare „wet“ and „dry“ preparation with either
transverse or longitudinal cut method.WHY?To improve specifications related to alternative
methods by getting more data from accredited / non-accredited laboratories and monitoring results achieved by „different“ methods.
A1. Introduction of new methodsComparative TEZ tests of Abies
22 labs interested (13 ISTA / 9 non ISTA)9 labs experienced and prefer „wet“ method4 labs prefer „dry“ (vacuumed) method9 labs no experience with Abies (5 ISTA labs)
Recent situation1 seedlot of stored seedsDistribution of samples for „wet“ and „dry“ preparation method + germination test (21 days at 3/5 °C, 28 days at 20/30 °C)
STA – data evaluation
A1.Comparative TEZ tests of Abies
A2. Introduction of new speciesRENGER
(National Network on Germplasm)
A2. Introduction of new speciesCalabria Calabria RegionRegion
7099,9ND0,05CISTUS CRETICUS L.
8699,5ND0,28CALICOTOME SPINOSA (L.) Link
ND99,6ND0,06ADENOCARPUS COMPLICATUS (L,) Gay G.
6199,9730,515JUNIPERUS OXYCEDRUS L. subsp. MACROCARPA (S. et S.) Ball
3199,9440026,2QUERCUS FRAINETTO Ten.
7898,912,24,8HIPPOPHAE RHAMNOIDES L.
8499,111,60,135ROSA SEMPERVIRENS L.
7199,51,60,15CISTUS SALVIFOLIUS L.
6999,51,20,15CISTUS SALVIFOLIUS L.
8299,96,20,0036CALICOTOME SPINOSA (L.) Link
8999,95,90,06CYTISUS VILLOSUS Porret
6599,91,50,15CISTUS SALVIFOLIUS L.
8499,95,90,01TELINE MONSPESSULANA L.
8999,96,40,03CYTISUS VILLOSUS Porret
GerminationPurity1000 WProductionSpecies
A2. Introduction of new speciesCalabriaCalabria’’s s SpeciesSpecies
A2. Introduction of new speciesMonte BaldoMonte Baldo
Cistus albidus L.MEMORANDUM OF COLLABORATIONORTO DI NOVEZZINA (Vr)
• VERBASCUM PHOENICEUM• PULSATILLA ALPINA• PRIMULA SPECTABILIS• ANTHYLLIS MONTANA• TRIFOLIUM RUBENS• AQUILEGIA EINSELEANA• AQUILEGIA ALPINA• ARGYROLOBIUM ZANONII• DIGITALIS GRANDIFLORA • ACHILLEA OXYLOBA• FUMANA PROCUMBENS • MALVA ALCEA • NEPETA CATARIA• POTENTILLA ERECTA
CALLIANTHEMUM KERNERANUM Freyn
Phillyrea latifolia L.TTZ : 81% viable seeds
Phillyrea latifolia L.
Cistus albidus L.
Pretreatment : 90 sec at 120°C Germination : 91% (10 gg at 15/25°C)
Cistus albidus L.Germination : 98% (7 gg at 20°C)
Gypsophila papillosa Porta.
Area : 2.5 Kmq
Gypsophila papillosa Porta.Gentiana lutea L.Pretreatment : GA3 0.2 mMGermination : 95% (10 gg at 15/25°C)
C. Workshop and SeminarC. Workshop and Seminar5^ FTS5^ FTS WorkshopWorkshop
Peri 12Peri 12--14 14 JuneJune 20082008
20 participants13 countries
FTSFTS WORKSHOPWORKSHOP12 JUNE 12 JUNE -- FIRST DAYFIRST DAY
chairchair: : SteffiSteffi KrKräämermerActivity report about FTS Activity report about FTS ((Z. Z. ProchazkovaProchazkova))
TEZ test (TEZ test (S. S. KrKräämermer))theoretical aspects + preparation of theoretical aspects + preparation of Abies alba,Abies alba,Acer Acer pseudoplatanuspseudoplatanus,, CarpinusCarpinus betulusbetulus andandCrataegusCrataegus monogynamonogyna seedsseeds
TetrazoliumTetrazolium
FTSFTS WORKSHOPWORKSHOP12 JUNE 12 JUNE -- FIRST DAYFIRST DAY
chair: Steffi KrämerDesiccation tolerance in seed of the New Zealand Flora
(C. McGill)Oak testing in Austria (I. Strohschneider)Discussion on moisture sample sizes (Z.Prochazkova) Discussion on Jatropha curcas (F. Gorian)
FTSFTS WORKSHOPWORKSHOP13 JUNE 13 JUNE -- SECOND DAYSECOND DAY
chair: Leena PietiläSampling (L. Pietilä)theoretical aspects practical aspects with Acer pseudoplatanus, Abies alba, Carpinus betulus and Crataegus
monogyna
TEZ test - evaluation : Crataegus monogyna,Abies alba (S. Krämer)
SamplingSampling
FTSFTS WORKSHOPWORKSHOP13 JUNE 13 JUNE -- SECOND DAYSECOND DAY
Sampling: sample for moisture content Sampling: sample for moisture content determination; sample reduction determination; sample reduction ((L. L. PietilPietilää))
TEZ testing on dormant California native TEZ testing on dormant California native shrubs (shrubs (M.AberleM.Aberle))
FTSFTS WORKSHOPWORKSHOP14 JUNE14 JUNE--THIRD DAYTHIRD DAY
chairchair: : ZdenkaZdenka ProchazkovaProchazkova
MOI test: theoretical and practical aspects (S. Pasquini)
MOI test: quality assurance and ISTA Moisture Committee (C. McGill)
Measurement of water activity on forest tree seed(F. Colas / P. Baldet)
TEZ test – evaluation: Acer pseudoplatanus;Carpinus betulus (S. Krämer)
Visit at CNBF Peri
Workshop Evaluation
1st TROPICAL FOREST TREE SEED WORKSHOP, 2009 – Curitiba, Brazil:
Approved Working Programme and Membership of ISTA Committees 2007-2010
Local Organizer: Antonio Medeiros, Member of ISTA Forest Tree and Shrub Seed CommitteeABRATES Forest Tree and Shrub Seed Committee Chair
• Date August 28 - 31, 2009
• Topics Physiology and Methodology
• LocationEstação Embratel Convention Center, Curitiba, Estado do Paraná - Brazil
Organizers
Fabio Gorian – ISTA, Member of Forest Tree and Shrub Seed Committee
Isolde Ferraz – ABRATES-Member of Forest Tree Seed Committee; ISTA-Member of Forest Tree and Shrub Seed Committee
Osvaldo Ohlson – ABRATES-Member of Forest Tree Seed Committee.
José França-Neto – ABRATES-Finance Director; ISTA-Member of Tetrazolium Committee
Goals- training and disseminationof knowledge in tropical treeseed science and technology
- to improve relationships between ISTA andtropical seed analysts from Central and South America
- to exchange experiences between ISTA and tropical seed analysts
Promotion and Organization- ISTA- ABRATES - BRAZILIAN MINISTRY OF AGRICULTURE, LIVESTOCK AND SUPPLY
Invitations (22)Guatemala (1)Honduras (1)Nicaragua (1)Costa Rica (1)Panamá (1)Colombia (1)Equador (1)Peru (1)Brazil (12)
Programme (draft )Purity for tropical forest tree seedTetrazolium testGermination testTropical forest tree seed storage
Technical Visiting to the Atlantic Rain Forest sites** Guides from Municipal Botanical Museum of Curitiba
and Municipal Botanical Garden of Curitiba
http://www.corpoforestale.it/cnbfperi
Rain Forest BiodiversityRain Forest Biodiversity• Rain forest in the world
Only 6% Earth surface but they have more species that in the rest of the world.
Rain forest in Amazon Region1 Ha (100mx100m) more than 1.500 differents
species of plants, more than UK (244.820 km²)
Tree species diversity (number)Amazon Region4.000 - 5.000North America ~650Central Europe ~50
Rain Forest BiodiversityRain Forest Biodiversity
EthiopiaEthiopia
UgandaUganda
RwandaRwanda
BurundiBurundi
TanzaniaTanzania
• ISTA secretariat asked toour FTS Committee to try totest this economic and enviromental importantspecies.
• The questions are:1. Is there anyone who is
experienced with Jatropha?2. Is there anyone who is
interested in it?
JatrophaJatropha curcascurcas L.L.(Barbados (Barbados nutnut or or PhysicPhysic nutnut))
Jatropha curcas, is a perennial poisonousshrub (normally up to 5m high) belonging to the Euphorbiaceae.
It is an uncultivated non-food wild-species.
• The plant, originating in Central America, whereas it has been spread to other tropical and subtropical countries as well and is mainlygrown in Asia and in Africa.
• It is used as a living fence to protect gardensand fields from animals. It is resistant to a high degree of aridity and as such does not compete with food crops.
• The seeds contain 30% oil that can be processed toproduce a high-quality biodiesel fuel, usable in a standard diesel engine.
• Fruits : fruits are produced in winter, or it may produce severalcrops during the year if soil moisture is good and temperatures are sufficiently high.
• Seeds: the seeds become mature, when the capsule changesfrom green to yellow
• It is usually multiplied by cuttings, because this gives fasterresults than multiplication by seeds.
Fruits and Seeds
• If planted in hedges, the reported productivity of Jatropha is from 0.8 kg. to 1.0 kg. of seed per meter of live fence. The seed production isaround 3.5 tons / hectare (Seed production ranges from about 0.4 tons per hectare in first year to over 5 tons per hectare after 3 years).
Jatropha curcas have limitednatural regeneration and artificially propagated by meansof seed. It is a cross-pollinatedand propagation through seed
lead to lot of genetic variability in term of growth, biomass, seed yield and oil content. Besides, the problems of low viability and recalcitrant nature of oil seeds limits the sexual propagation. On the other hand, Clonal techniques will help in overcoming the biological problems in hinderingmass propagation of this Tree BorneOilseed species.
Jatropha curcas(and other species of the genus)
GerminationGermination -- Charlotte Seal + Hugh PritchardViability – TEZMoisture content - MOI
Thank you for your attention