57
FUSION 4000 & 6000 USER MANUAL
FUSION 4000 & 6000USER MANUAL
2
EU Declaration of Conformity (DoC)....................................................................................................................6
General Description...............................................................................................................................................7
Warranty and Repair Information........................................................................................................................9
Warranty Information................................................................................................................................9
Repair Facilities and Parts........................................................................................................................10
Dead Pixel Policy......................................................................................................................................10
Serial Numbers.........................................................................................................................................10
Calibrations.........................................................................................................................................10
Product Overview..................................................................................................................................................11
Principle of Operation..............................................................................................................................11
Controlled Elements.................................................................................................................................12
Specifications...............................................................................................................................................15
Pump Setup...........................................................................................................................................................17
PoweringOn/OffthePump....................................................................................................................17
Loading a Syringe.....................................................................................................................................18
AdjustingtheSafetyNut/StallRod........................................................................................................21
Pump Operation......................................................................................................................................22
Mode Selection Screen............................................................................................................................24
Pressure&TemperatureSettings(Fusion6000only).......................................................................25
Basic Mode............................................................................................................................................................26
Syringe Selection......................................................................................................................................26
Volume......................................................................................................................................27
Infusion/Withdrawl.................................................................................................................................28
Flow Rate..................................................................................................................................................28
Start-Time Delay......................................................................................................................................29
Step Loop..................................................................................................................................................29
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Options...........................................................................................................................................................30
Flow-Rate Units........................................................................................................................................30
Options....................................................................................................................................31
Enable Volume Lock.................................................................................................................................31
Multi-Step Mode...................................................................................................................................................32
Setup.......................................................................................................................................32
Syringe Selection.....................................................................................................................................32
Total Steps................................................................................................................................................33
Loop All.....................................................................................................................................................33
Sub-Loops...........................................................................................................................................33
Flow-Rate Units........................................................................................................................................34
Step Parameters...................................................................................................................................................34
Volume.......................................................................................................................................35
Pump Control.......................................................................................................................................................36
System Settings....................................................................................................................................................39
Pump Control by Computer...............................................................................................................................41
Cable Requirements............................................................................................................................................41
Connecting ............................................................................................................................................................41
COM Port Settings....................................................................................................................................42
Pump Commands................................................................................................................................................43
ChemyxPumpControllerPrograms.................................................................................................................48
Pump Maintenance.............................................................................................................................................49
Lubricating thePump.............................................................................................................................49
Lubrication Instructions.........................................................................................................................49
Approved Lubricants...............................................................................................................................49
PumpAccessories................................................................................................................................................50
4
Appendices.................................................................................................................................................52
AppendixA:SyringeVolume/DiameterRefference............................................................................52
AppendixB:AvailableParts....................................................................................................................56
AppendixC:ContactInformation...........................................................................................................57
5
INTELLECTUAL PROPERTYAllIntellectualProperties,asdefinedbelow,ownedbyorwhichisotherwisethepropertyofChemyxInc.oritssuppliersrelatingtotheChemyxsyringepumps,includingbutnotlimitedto,accessories,
partsorsoftwarerelatingthereto(ChemyxSyringePumps),areproprietarytofederalandstatelaws,andinternationaltreatyprovisions.IntellectualPropertyincludesbutisnotlimitedto,inventions
(patentableorunpatentable),patents,tradesecrets,copyrights,software,firmware,computerprograms,andrelateddocumentationandotherworksofauthorship.Moreover,youagreethatyouwill
not,andwillnotattemptto,modify,preparederivativeworksof,reverseengineer,disassembletheChemyxsyringepumps,decompileorotherwiseattempttocreatesourcecodefromtherelated
software/firmware.NotitletoorownershipinIntellectualPropertyistransferredtoyou.AllapplicablerightsoftheIntellectualPropertyshallremainwithChemyxanditssuppliers.
6
Declaration of Conformity
ThePDFversionofthelatestDoCcanbedownloadedatwww.chemyx.com/support
7
General Description
PleasereadthefollowingsafetyprecautionstoensurepersonalsafetyandoperationallongevityoftheChemyxsyringepump.Chemyxisnotresponsiblefortheequipmentifusedinamannernotspecifiedbythemanufacturer;warrantycoverageprovidedbytheequipmentmaybedroppedasaresult.
CHEMYX PRODUCTS ARE NOT APPROVED FOR CLINICAL USE ON HUMANS.
Chemyxisnotresponsiblefortheuseofpowersuppliesoutsidethestatedelectricalspecificationsorfailuretoswitchthepowerconverterfrom220Vto110Vwhileinthe220Venvironmentorvice versa.
Theproductshouldbeproperlygrounded.
Use Proper Power Supply
Ground Product
Safety Information
WarrantycoveragewillbelostifthepumpisopenedwithoutauthorizationfromChemyx.Donottouchanyelectricalconnectorsonorintheproduct.
Eventhoughthepumpcanoperateatextremelyfastspeeds,theusermustdeterminetheproperflowrateforanygivenapplication.Forinstances,pumpingat90mL/minusinga20-gaugeneedlewillcausestallsand/orpotentialburstingofthesyringe.Chemyxisnotresponsibleforanydamagethatmightresultfromsituationssimilartotheexampleabove.
Do Not Open The Pump
Do Not Operate With Suspected Failures
8
Donotplacefingersbetweenthepusherblockandtheendblockwhilethepumpisrunning.
Readalllabelsontheproducttoensureproperusage.
Theuserisresponsibleforwettinggroundglasssyringesandsettingandtighteningthesafetycollar/bar appropriately.
Pinch Hazard
Observe all Warning labels on Product
Chemyx is not Responsible for Syringe Damage
9
Warranty and Repair InformationChemyxprovidesatwo-yearwarrantyfromtheshipmentdateforitspumpsagainstdefectsinmaterialsandworkmanship.Chemyxwillrepairanyproductthatprovesdefectiveduringitsstatedwarrantyperiod.
Theforegoingwarrantywillnotapplytodamageresultingfrom:
YoumustcontactChemyx(call+1281-277-5499orvisitwww.chemyx.com/contact-us)beforereturningaproduct.ChemyxwillissueaReturnAuthorization(RA)numbertoyou.
Return products to:
ChemyxInc.
10905CashRoad|Stafford,TX77477USA.
Warranty Information
Improper or inadequate maintenance or operation.
Unauthorizedmodificationormisuseoftheproduct.
Operationoutsidetheelectricalspecificationsfortheproduct.
Operationoutsidethetemperaturespecificationsfortheproduct.
User-inducedinternalandexternalcontaminationsoftheinstrument.
Failure to use proper surge protection.
Improperproductreturn,packaging,andshipping
Removing serial number from syringe pump
10
Chemyxcanrepairanysyringepumpwithoutmajordamage.YoumustcontactChemyx(call+1281-277-5499orvisitusatwww.chemyx.com/contact-us)beforeshippingaproductforrepair.ChemyxwillissueaReturnAuthorization(RA)numbertoyou.
Return products to:
Chemyx Returns
10905CashRoad|Stafford,TX77477USA
DuringtheLCDMonitormanufacturingprocess,itisnotuncommonforoneormorepixelstobecomefixedinanunchangingstate.Thevisibleresultisafixedpixelthatappearsasanextremelytiny,darkorbrightdot.Inalmosteverycase,thesefixedpixelsarehardtoseeanddonotdetractfromdisplayqualityorusability.Adisplaywiththreetosevenbrightordarkdotsisconsiderednormalandwithinindustrystandards.Ifyourscreendisplaysmorethansevendeadpixelsduringthewarrantyperiod,yoursystemwillqualifyforwarrantyreplacement.PleaseseetheLimitedWarrantysectionfordetailsonhowtoactivatea warranty claim.
Theserialnumberislocatedontheback,topleftcornerorcenterofthepumpunderasmallbarcode.Removaloftheserialnumberlabelwillvoidthewarranty.
Chemyxpumpsarepre-calibrateduponarrivaltoyoursite.Allcalibratedparametersarewithinstatedaccuracyandprecisionspecifications of the pump. Although the pump is highly accurate, syringes of differentmaterials can attribute significantvolumeerrors.Typically,theseerrorscanrangefrom1%forglasssyringesto5%forplasticsyringes.Chemyxisnotresponsiblefor errors generated from syringes.
Repair Facilities and Parts
Dead Pixel Policy
Serial Numbers
Calibrations
11
Product Overview
The Chemyx Fusion product line serve a wide variety of applications. The Chemyx Fusion 4000 is configured with twoindependentlycontrolledsyringepumpchannelswhiletheChemyxFusion6000isahigh-pressuresyringepumpdesignedfordosingviscoussolutions,andsimi-solidmaterialinjectionsagainstpressure.Theunits’flexibilitywithsyringetypesandsizesandanadvancedsoftwareinterfacemakethishigh-pressuresyringepumpsintegrableintoanyoperationorlaboratoryworkflow.Thoroughlydurablebothinsideandout,theChemyxFusionsyringepumpsaredesignedtoprovideyearsofconstantandreliable service.
Asyringepumpisasmall,positive-displacementpumpusedtograduallytransferprecisevolumesoffluid.AllChemyxFusion-seriessyringepumpsaredrivenviaasteppermotor.Thissteppermotorpreciselyturnsaleadscrewthatisthreadedthroughapusherblock,whichcausesthepusherblocktomove.Whenthepumpisininfusionmode,thepusherblockpushesagainsttheplungerofasecuredsyringe,causingthefluidtobeejectedatanaccurateandpreciserate.
Ifthepumpiscapableofwithdrawal,theplungerofthesyringeisheldbybracketsonthepusherblock.Whenthesteppermotorturnsintheoppositedirection,thepusherblockmovessuchthatthesyringeplungerispulled,thusdrawingfluidintothesyringe.
FortheFusionSeriessyringepumps,thepusherblockmovestotherightforinfusionandtotheleftforwithdrawal.
Principle of Operation
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AllFusionSeriespumpshaveasingleleadscrewthroughwhichthepusherblockisthreaded.Thepusherblockmoveswhentheleadscrewturns.Thetwoguiderodskeepthepusherblockhorizontalandperfectlyperpendiculartotheleadscrew.FortheFusion4000thescrewknobonthepusherblockdisengagesthepusherblockfromtheleadscrew,whichallowsthepusherblocktobeeasilymovedtoanewpositionaslongasthebuttonisheld.Tightingtheknobwilllockthepusherblockbackinplace.AsfortheFusion6000thepusherblockcanonlybemovedbackandforthfromthepumpscreeninthemenusection.
Syringesareplacedonthesyringe-holderblockinthev-shapedgrooves,orchannels,andheldsecurelyusingsyringeclamps.TheFusion4000hastwoindependentsyringechannels,whiletheFusion6000hasonelargesyringechannels.
TheFusion4000hasasafetycollarwhiletheFusion6000hasastallrodtohelpprotecttheintegrityofsyringesbykeepingthepusherblockfrompushingonacompletelydepressedsyringe.
Controlled Elements
Syringe Holder Block
Safety Rod Lead Screw
PusherBlock
Syringe Clamp
SyringeChannelsFusion 6000 pump components
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TheFusionSeriesusesabright,4.25”,resistive,LCDtouchscreenwithanintuitivegraphicaluserinterface(GUI)forsettingupdetailedpumprunmethods.ButtonsandentryfieldswithintheGUIareallselectableusingthetouchscreen.Asaresistive,LCDtouchscreen,theinterfacecanbeoperatedwithanyobject,notjustthetouchofafingertip.Selectingelementsbytouchonlyrequiresasmallamountofpressure;however,pleasedonotusesharpobjectsastheymaydamagethescreen.
TheFusionSeriesalso includesphysicalnavigationcontrols,allowingusers tomanuallymaneuverbetweenselectableGUIelementswithouttouchingthescreen.InputtingnumbersandletterswithintheGUIisdoneusingthephysicalnumerickeypad.Thephysicalpumpcontrolkeysareusedtostart,pause,andstoppumpactivity.
ThepowerLEDandpump-controlLEDsallowuserstoeasilydiscernwhetherthepumpisonandwhetheritiscurrentlyrunning,paused, or stopped.
Navigation Keypad
Front Panel of Pump
Controls
Power LED Pump Controls LCDTouchscreen Numeric Keypad
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TheFusion4000 iscapableofboth infusionandwithdrawlwithtwoindependentlycontrolledpumps.TheFusion4000uses adjustable retainingbraketson thepusherblock andthe syringeholderblock, that keepeach individual syringechannelinplaceduringinfusionandwithdrawl.
InadditiontohavingallofthesamefeaturesandcomponentsoftheFusion4000,theFusion6000iscapableofbothinfusionandwithdrawalonasinglepumpchannel.TheFusion6000also uses an adjustable retaining brackets on the pusherblockand thesyringeholderblock thatkeep thesyringe inplaceduringbothinfusionandwithdrawal.
Bracket Release Knob
Bracket Release Knob
Flange Retaining Bracket
Flange Retaining Bracket
Plunger Retaining Bracket
Plunger Retaining BracketBracket Release Knob
Fusion 4000 Specific Components
Fusion 6000 Specific Components
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Specifications
Specification Fusion 4000 Fusion 6000
Movement Modes
Infusion/Withdrawal
Infusion/Withdrawal
SyringeSizesW(Min/Max) 0.5μL/140mL 0.5μL/255mL
Flow Rate Range (Smallest Syringe)
0.0001 to 1.08μL/min
0.0001 to 1.5679μL/min
Flow Rate Range (Largest Syringe)
0.0002 mL/minto 128 mL/min
0.0002 mL/minto 128 mL/min
DriveMecha-nism
0.9°steppermotorwith1/128 microstepping
0.9°steppermotorwith1/48 microstepping
Step Resolution 0.012μm/step 0.046μm/step
Nominal Linear Force 29kg(65lbs) 227kg(500lbs)
Accuracy ±<0.35% ±<0.35%
Reproducibility ±<0.05% ±<0.05%
Operation Temp 4°C to 40°C (40°F to 104°F)
4°C to 40°C (40°F to 104°F)
Storage Temp -10°C to70°C (14°Fto158°F)
-10°C to 70°C(14°Fto158°F)
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Specifications (Continued)
Specification Fusion 4000 Fusion 6000
Relative Humidity 20%-80% 20%-80%
Data Ports RS-232 (DB9 and USB-B), TTL (USB-B)
RS-232 (DB9 and USB-B), TTL (USB-B)
Height 16 cm (6.3 in.) 18 cm (6.9 in.)
Width(Length) 27 cm (10.8 in.) 27 cm (10.8 in.)
Depth 20 cm (7.8 in.) 21 cm (8.2 in.)
Weight 4.5kg(10lb) 5.9kg(13lb)
Screen 4.25inchdiagonalLCD 4.25inchdiagonalLCD
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Pump SetupToprovidepowertothepump,anIEC60320C13cableshouldbepluggedintothepowersocketonthebackofthepump.Thecableshouldbepluggedintoanappropriatepowersource(110Vor220V)withpropergrounding.Adjustthevoltage-converterswitchonthebackofthepumpsuchthatthevaluedisplayedontheswitchcorrespondswiththevoltageofthepowersupply.
Thepowerswitchforthepumpislocatedontheback,abovethepowersocket.Uponflippingtheswitchtothe“on”position,theblueLEDpowerlightonthefrontofthepumpwillimmediatelyturnon,alongwiththeredLEDlightontheStopkey.Inafewseconds,abeepwillsound,andtheLCDscreenwilldisplayaninitialstartupscreen.
The startup screen displays the version of the firmwarecurrentlyinstalledonthepumpatthetopofthescreen.Thestartupscreenwilldisplayforapproximatelyfivesecondsasindicatedbythecountdownontherightofthestartupscreen.
Afterthestartupscreen,theLCDscreenwilldisplaythelastmodethepumpwasin.
Powering On/Off the Pump
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TheFusion4000seriescanaccommodateuptotwosyringes,whiletheFusion6000canholdonelargesyringeupto200mL.TheFusion6000canbeexpandedto4channelsusingtheChemyx4-Channel100mLSyringeRackaccessory.ThevolumeoftheFusion4000syringescanvaryfromaminimumof0.5μLuptoamaximumof140mLandtheFusion6000syringecanvaryfrom0.5μLto200mL.Syringesofanymaterial(e.g.,glass,plastic,stainlesssteel,etc.)canbeused;however,someusecasesmayrequiresyringesthataremoreaccurateanddurable.
Loading a Syringe
Fusion 4000
2 31 4
1. Ifnecessary,moveandtightentheinfusionsafetycollar(seeAdjustingtheSafetyCollar).
2. Whileunscrewingtheknobonthepusherblock,theblockwillreleasetoslidethepusherblockallthewaytotheright.
3. Loosenthescrewknobsfortheflangeretainingbracketandadjustthebracketsuchthattheflangeofthesyringebarrelwillfitinbetween.Donottightenthebracket,yet.
4. Pulluponthespring-loadedsyringeclampandplacethesyringeinoneofthechannelsoftheholderblock.Ensurethattheflangeofthesyringebarrelisflushagainsttheedgeoftheholderblock.Slowlylowerthesyringeclamptosecurethesyringe in place.
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5 76 8
Fusion 4000 (Continued)
5. Loosenthebracketclampknobfortheplungerretainingbracketandadjustthegapsuchthattheplungercapofthesyringemayfit.
6. Unscrewtheknobtoreleasetheblockandslidethepusherblocktotherightuntilitisflushagainstthesyringeplunger.Theplungercapforthesyringeshouldfitinthegapbetweenthebracketandthepusherblock.
7. Tightenthebracketclampknobfortheplungerretainingbrackettosecurethesyringeplungerinplace.
8. Movetheflangeretainingbracketflushagainsttheflangeofthesyringebarrelandtightenthebracketclampknobstosecurethesyringebarrelflangeinplace.
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1. If necessary,move and tighten the infusion safety rod(seeAdjustingtheSafetyRod).
2. TomovethepusherblockusetheForwardandReversefucnctionsthatcanbefoundonthemenuscreenofthepump.Movethepusherblockallthewaytotheright
3. Unscrewthesyringeclampandplacethesyringeinoneofthechannelsofthesyringe-holderblock.Ensurethattheflangeofthesyringebarrelisflushagainsttheedgeofthesyringe-holderblock.Slowly lowerandscrewthesyringeclamptosecurethesyringeinplace.
4. Usethemenuforwardandreversefunctionstomovethe
Fusion 6000
2
1
3
5 6 78
pusherblocktotheleftuntilitisflushagainstthesyringeplunger.
5. Loosenthebracketclampknobfortheplungerretainingbracketandadjustthegapsuchthattheplungercapofthesyringemayfit.
6. Usethemenuforwardandreversefunctiontomovethepusherblocktotheleftuntilitisflushagainstthesyringe
plunger.Theplungercapforthesyringeshouldfitinthegapbetweenthebracketandthepusherblock.
7. Tightenthebracketclampknobfortheplungerretainingbrackettosecurethesyringeplungerinplace.
8. Movetheflangeretainingbracketflushagainsttheflangeofthesyringebarrelandtightenthebracketclampknobstosecurethesyringebarrelflangeinplace.
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TheFusionSeriespumpcangenerateasignificantamountof linearforce.Whilethis isextremelyusefulfordispensingviscousfluidsathigherflowrates,thehigherlinearforceoftheFusionseriescanalsopotentiallydamagethesyringesinuse.Onesourceofpotentialdamageofasyringemayoccurwhentheplungerofthesyringeiscompletedepressed,andthepusherblockisstillpushingagainstthesyringe.Dependingonthematerialofthesyringe(i.e.,glassorplastic)andthethicknessoftheplunger,thepressurefromthepusherblockcouldbend,distort,orjustbreakthesyringe.
Toprotectagainstthispotentialproblem,Fusion4000syringepumpsusesafetycollarsandFusion6000useastallrodtophysicallystopthepusherblockbeforeitcancompletelydepressthesyringeplunger.Whenapusherblockhitsthesafetymechanismandcannolongermoveforward,thepumpwillstoprunning,emitalongbeep,andenterpump-stalledmode.
Adjusting the Safety Nut/ Stall Rod
Adjusting the safety collar requires theuseof thehex key,convenientlystoredonthebackleftofthepump,rightabovethe power switch. Loosening the hex screw on the safetycollarallowsforthecollartobeslidfreelyalongtheguiderodandthensecurelytightenedtosetthepositionofthesafetycollar.Thepositionofthesafetycollarshouldbesetslightlyfurtherfromthesyringe-blockholderthanthetopofsyringeplungerwhenfullydepressed.
TheFusion6000usesastall rod insteadofa collar to limitthedistance thepusherblockmay travel.By loosening thesafetybarclampknobontopofthepusherblock,thesafetybarcanbeslidleftorright.Thesafetybarshouldbesetsuchthatthe lengthofthebarexposedontherightsideofthepusherblock is longer thanthedistancefromthetopofafullydepressedsyringe plungertotheedgeofthesyringe-holderblock.Oncethesafetybarhasbeensettotheproperlength,tighteningthesafetybarclampknobwillkeepthebar from moving.
Fusion 4000
Fusion 6000
Caution:On the Fusion Series, during withdrawal, a syringe plunger can potentially be pulled out from the syringe, which could cause the contents of the syringe to leak out. Users should be especially aware of the length of the plunger and the point where the withdrawing pusher block will exceed that length. There are no safety collars on the Fusion Series to prevent too much withdrawal.
Stall Rod adjustment
Safety collar adjustment
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TheLCDscreendisplaysagraphicaluserinterface(GUI)fromwhichallthepumpsettingsandrunparametersareentered.Wheneveran itemiscurrentlyselected,thecolorschemeoftheelementtypicallyturnstoadarkershadeofthecolor.Forexample,thelightbluebuttonsturndarkbluewhenselected,andthewhitebackgroundsurroundinganunselectededitabletextfieldturnsbluewhenthefield isselectedandavailable forediting.Also,whenaGUIelement isselected, thetopbluebarwillshowtheactivityperformed(buttonsonly)orshowthelimitstothevaluesthatmaybeenteredfortheselectedfield(editablefieldsonly).
Selecting the itemsthatwillbemodifiedcanbedonetwoways: (1)using theLCDtouchscreenor (2)using thenavigationkeypad.
AlloftheelementsoftheGUIcanbeinteractedwithbytouch.Typicaltouchinteractionsaretappingafingeronabuttonortextfieldorslidingafingertoscroll.Asaresistivetouchscreen,theseinteractionsarenotrestrictedtoonlyunglovedfingertips.Manytypesofobjectscanbeusedtoselectitemsonthescreenwithonlyveryslightpressure,butavoidusingsharpobjects,astheymaypermanentlydamagethescreen.
ElementsoftheGUImayalsobeselectedusingthenavigationkeypadonthefrontoftheinstrumenttomovebetweentheelements.
Toactivate(or“press”)abuttonusingthenavigationkeypad,selectthebuttonelementsuchthatitishighlightedandthenpresstheENTERkeyatthecenterofnavigationkeypad.EditablefieldscanimmediatelybeediteduponselectionwithouttheneedtopresstheENTER key.
Pump Operation
Operating Interface
Limitsforselectedfields
Selected Fields
Unselected Field
Tip:Users may find the navigation keypad easier to use for scrolling through items in a scrollable list, such as the list of syringes in the syringe library.
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AllofthescreensintheFusionSeriesGUIexistinahierarchy,withtheModeSelectionscreenatthetop.Enteringanewscreenisperformedsimplybyactivating,ortapping,abuttonoriconthatopensthenewscreen.
Mosteditablefieldswillrequiretheusertoenternumbers,whichisdoneusingthenumerickeypadonthefrontoftheinstrument.
Uponfirstselectinganeditablefield,pressinganynumberwillfirstcleartheoldvalueandreplaceitwiththenewvalue.Thisonlyapplieswhenfirstselectingthefield.Onafieldthathasalreadybeenselectedandedited,anyfurthereditswilljustappendthenumbertotheend.Insuchacase,toclearthecurrentvalueinthefield,pressthewhiteCkeyonthenumerickeypad.
Valuesenteredinaneditablefieldareaccepteduponpressingthe ENTER key, selecting a newelement,orpressingtheStart key.Valuesthatexceedthe limitsof theeditablefield(showninthebluebaratthetopofthescreen)areautomaticallycorrectedtobewithinthelimitsuponacceptingthevalue.
Toreturntothepreviousscreen,taporactivatethered“X”buttoninthetoprightofthescreen.Thiswill“close”thecurrentscreen,andreturntothescreenthatwasusedtoentertheclosedscreen.ThiscanalsobeaccomplishedbypressingtheStopkeyonthefrontoftheinstrumentwhenthepumpisnotrunning.
Navigating Between Screens
Entering Numbers
PhysicalNumericKeypad
24
Mode Selection ScreenThe Mode Selection screen is the top levelmenufortheFusionseriespumps.TheFusion4000 screen allows users to select between pumpsandCycleMode.TheFusion6000screenallows users to select flow and pressure andtemperaturecontrolingprofiles.
Fusion 4000 Mode Selection
Fusion 6000 Mode Selection
25
Pressure & Temperature Settings (Fusion 6000 Only)TheTemperature Settings screen allows you tocontrol theoptional syringeheaters for theFusion6000pump.Theoptionsonthescreenallowyoutostarttheheaterwithflow,controltemperature, pre-heat the syringe and thenstartthepumpaswellassettingatimeronthesyringeheater.Whileusing the syringeheatertheonlywaytoturnthehearteroffistounplug,to set temperatre below room temperature or usetheheatertimer.
The heater is started and stopped with everystartandstopofflow,pausingthepumpdoesnot stop the heater. The “Pre-heat then startflow”optionwillpause theflowandwiatuntilthe target temperature is reached then startflow;asepcialstatustextwillbeshownifactive.
The Pressure Settings screen allows you to control the prssure for the syringe for theFusion6000pump.Theoptionsonthescreenallowyou to stopflowat a targetpressureorholdatargetpressure.Whileusingthepressurefunctionalitytheonlywaytoturnthepressuresensor off is to unplug or to set temperatureveryhigh.
When“Stopflowattargetpressure”optionwilldisplay a special stop status text if pressure is reached. The option “Hold target pressureand modulate flow” when selected will slowdownfloaorspeedupflowtotargetflowrateto maintain constant pressure. This will alsodisplay a special status message if active and removal of edit rate.
Fusion 6000 Temperature Setting
Fusion 6000 Pressure Setting
26
Basic Mode (or Single-Step Mode) is used for applications that do not require advanced automation, variable volumeand rate settings, step looping, or ramping rates.
The Basic Mode screen can be reached by tapping on thewaterdropleticonontheMode Selection screen.
Basic Modeallowsuserstoselectthesyringebeingused,tosetavalidvolumeandrate,andtochoosewhethertoinfuseorwithdraw.
ThepumpcanonlybestartedinBasic ModewhiletheBasic Mode screen is displayed.
Basic Mode
Syringe Selection
Basic Mode Screen
Foreveryapplication,thefirstrequiredstepforsetupistoselecttheinnerdiameter(ID)ofthesyringe(s)beingused.Thisvalueisimportantforthesyringepumptoaccuratelycalculatethetransferrateandthetotaltransferredvolumefortheliquidinthesyringe.Therearetwoapproachestoenteringthisvalue:(1)enteracustominnerdiameteror(2)usethebuilt-insyringelibraryto import an inner diameter.
Enteringacustominnerdiameter(ID)isassimpleastypingintheIDinmillimetersintheSyringeeditablefield.ThemaximumandminimumvaluesfortheIDaredisplayedinthebluebaratthetopofthescreenwhiletheSyringeeditablefieldisselected.Thisvalueisalsolimitedtoonlythreedecimalplaces,whereanyadditionaldecimalsortrailingzeroesaredropped.IfthevalueoftheVolumefieldissmaller/largerthanthemin/maxvolumeallowedfortheinputinnerdiameter,thevalueintheVolumefieldwillbeadjustedtobewithinrange.Otherwise,nochangestothevolumewilloccur.
TappingtheFind SyringebuttontotherightoftheSyringe editablefieldwillopenanewSyringe Library screen. Syringe manufacturers are found in the list on the left. Selecting amanufacturerwill display all of the syringes available fromthatmanufacturerinthelistontheright.Thelistofsyringestypicallyindicatesthevolumeofthesyringefirst,followedbytheinnerdiameterofthesyringe.Bothlistscanbescrolledthroughbydraggingafingeronthescrollbar;however,theeasiestapproachinthisscreenmaybetousethenavigationkeypadtoscrollthroughthelists.Alistofthesyringesinthelibrary can be found in Appendix A.
Syringe Library Screen
27
Whenaparticularsyringeisselectedfromthelistontheright,anImport buttonwillappearnexttotheselecteditem.Tappingthe Importbutton (orpressingtheEnterkeyonthenavigationkeypad)will import the innerdiameterassociatedwiththeselectedsyringeandexittheSyringe Library screen.
Uponimportofasyringefromthesyringelibrary,theSyringeeditablefieldwillbeupdatedtothenewinnerdiameter,andtheVolumeeditablefieldwillbeupdatedtothevolumeoftheimportedsyringe.PleasenotethatthevolumeunitsandvaluesfortheVolume and RatefieldsmaychangetomatchtheunitsofvolumedisplayedforthesyringeintheSyringe Library.
Volume
TheVolumefieldrepresentsthetotalvolumeofliquidthatthepumpwilltransfer(infusionorwithdrawal)beforestopping.Theamountoftimethatthepumpwillrunwiththesettargetvolumecanbedeterminedbydividingthevolumebytheflowrate.
Thevalueenteredcanbeanyintegerordecimalwithinthelimitsforthesyringe.Themin/maxvolumelimitscanbefoundinthebluebaratthetopofthescreenwhentheVolumefieldisselected.Anyenteredvaluebeyondtheselimitswillbeautomaticallyadjustedtotheminimumormaximumlimit,whicheveriscloser.Thetotalnumberofdecimalplacesislimitedtofive.
ThevolumecanbestatedinunitsofmLorμL.TheunitsusedforthevolumearedependentuponthesetunitsofvolumefortheflowrateintheOptions screen.
Tocontinuouslyrunthepumpandusetheentirevolumeofthesyringe,userscansetthevolumetothetotalvolumeofthesyringeortothemaximumlimit.However,usersarestronglyencouragedtosetthesafetycollar/bar(seeAdjustingtheSafetyCollar)sothatthepumpdoesnotpotentiallyruinthesyringe.
Tip:Because changing the values of the syringe ID may possibly change the values and units for volume and rate, selecting a syringe or entering the syringe ID should always be the first step when setting up a run.
Important Note:If more than one syringe will be used at the same time, it is recommended that the all of the syringes be of the same inner diameter. Because Fusion pumps calculate the rate that the pushing block moves based on the input syringe inner diameter and flow rate, syringes with larger or smaller inner diameters will transfer more/less volume than indicated by the GUI.
Important Note:The maximum volume limit for a syringe assumes that the length of the syringe is the length of the pump. Therefore, this maximum volume limit is almost always larger than the actual volume the syringe can hold.
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Flow RateTheFlow Ratefieldsindicateshowfastthepusherblockwillmove.Theamountoftimethatthepumpwillruncanbedeterminedbydividingthesetvolumebytheflowrate.
In Multi-Step Mode,theflowrateduringthestepcanbestaticorvariable,whichissetbytheratefieldsineachstep.Step 1 representstheflowratewhenthestepstartswhileStep 2representstheflowratewhenthestepends(seeStaticFlowRateandVariableFlowRateformoredetails)andsoforthtoothersteps.
Foreachfield,thevalueenteredcanbeanyintegerordecimalwithinthelimitsforthesyringe.Themin/maxflowratelimitscanbefoundinthebluebaratthetopofthescreenwhentheRatefieldisselected.Anyenteredvaluebeyondtheselimitswillbeautomaticallyadjustedtotheminimumormaximumlimit,whicheveriscloser.Thetotalnumberofdecimalplacesislimitedtofive.
Theratecanbestatedwithfourdifferentunits:mL/min,mL/hr,μL/min,andμL/hr.Adjustingtheunitswillaffectthemin/maxlimitsfortheRatefield.TheunitsforratecanbesetinOptionsduringthemulti-stepsetup.
Caution:Flow rate is often set for the needs of the experiment; however, users should be aware that setting high flow rates, especially for viscous fluids, may require high linear force beyond the limits of the pump which may cause the pump to stall out. Additionally, high flow rates can exert tremendous pressure within a syringe that may cause glass or plastic syringes to burst. In the case of high-pressure experiments, users should consider using Chemyx Stainless-Steel Syringes and the high-pressure Chemyx Fusion 6000 pump.
Infusion/Withdrawal
TheFusionSeriesiscapableofbothinfusionandwithdrawal.Onthesystems,anInfusion/WithdrawaltogglebuttonshouldappeartotherightoftheVolumefield.Thetextandarrowdirectiononthebuttonindicatesthemodethatiscurrentlysetanddirectionthatthepusherblockwillmoveuponstartingtherun.Tappingthebuttonwillswitchbetweenthetwomodes.
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Step LoopTheStep Loopfielddetermineshowmanyadditionaltimestosequentiallyperformtheparticularstep.Thisisthelowest-levelloopthatiterateswheneverthatparticularstepisreachedandcompleted.
TheStep Loopfieldtakesanyintegerfrom0to99.Avalueof0indicatesthatthemethodwillrunthesteponlyonce.Anyvalueabovezerorepresentsadditionaltimestorunthestep.
InthePump Runningscreen,thenumberofremainingloopsofthecurrentstepappearsontheright,nexttotherepeaticon.
Start-Time DelayTheDelayfieldrepresents theamountof time (inminutesandseconds) todelaystarting thepumpafter thecurrentstepbegins.
Whenenteringthisvaluewiththenumerickeypad,thedecimalkeyisusedtoindicatetheseparationbetweenminutesandseconds.
Toremovethedelay,simplypresstheclearkey(C)orsetthetimetozero.
Example:To set a delay of 1 min and 30 seconds, press the “1” key, then the decimal key, and finally the “3” and “0” keys.
Example:In a multi-step method of five steps (1, 2, 3, 4, 5) where Step 4 has a Step Loop of “2”. The method will run the following steps sequen-tially: 1, 2, 3, 4, 4, 4, 5. When the step loop is set to “2”, Step 4 is repeated twice more. If the entire method also has a Loop All setting of “1”, the method would run the following steps sequentially: 1, 2, 3, 4, 4, 4, 5, 1, 2, 3, 4, 4, 4, 5.
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Flow-Rate Units
TheFusionSeriesiscapableofbothinfusionandwithdrawal.Onthesystems,anInfusion/Withdrawaltogglebuttonshouldappearwhen theVolumefield is selected.Thebubble form indicates themode that is currentlysetanddirection that thepusherblockwillmoveuponstartingtherun.
Theflow-rateunitsdeterminewhichunitsofvolumeandtimewillbeusedfortheVolume and Ratefields.ItalsodeterminestheunitsforthePriming/Bolus Rate.ThissettinginBasic ModealsochangestheunitsforMulti-Step Mode.
Note:Even if the units have changed, the values within the Volume and Rate fields will not change, which will result in significant changes in the flow rate and volume transferred. Additionally, the min/max limits for these fields will also be changed, so if the current values for volume and rate exceed the new limits, they will be adjusted to be within those set limits.
Volume Settings screen
Options
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Options
Enable Volume Lock
Primingallowsfortheusertotemporarilyrunthepumptoremovedead/emptyvolumeinthesyringeneedleortubingpriortorunningtheexperiment.
The rateatwhichprimingoccurs is set in thePriming/Bolus Rate field. Thevalueenteredcanbeany integerordecimalwithinthelimitsforthesyringe.Themin/maxflowratelimitscanbefoundintheorangebaratthetopofthescreenwhenthe Priming/Bolus Ratefieldisselected.Anyenteredvaluebeyondtheselimitswillbeautomaticallyadjustedtotheminimumormaximumlimit,whicheveriscloser.Thetotalnumberofdecimalplacesislimitedtofour.
TheunitsforthePriming/Bolus RatearedeterminedbytheFlow-RateUnitssettinginOptions.Adjustingtheunitswillaffectthemin/maxlimitsforthePriming/Bolus Ratefield.
ToenterprimingmodeonFusionpumps,pressandholdtheStartkeywhileinthemainscreenofBasic Mode.
Thisfunctionhasbeendeprecatedandisnolongersupported.
Caution:Flow rate is often set for the needs of the experiment; however, users should be aware that setting high flow rates, especially for viscous fluids, may require high linear force beyond the limits of the pump which may cause the pump to stall out. Additionally, high flow rates can exert tremendous pressure within a syringe that may cause glass or plastic syringes to burst. In the case of high-pressure experiments, users should consider using Chemyx Stainless-Steel Syringes and the high-pressure Chemyx Fusion 6000 pump.
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Multi-Step Modeisusedformoreadvancedapplicationsthatmayrequirenumeroussteps,multipledifferentvolumeandratesettings, step looping, or ramping rates.
The Multi-Step ModescreencanbereachedbytappingontheMenuiconontheMode Selection screen.
Thepumpcanonlybestarted inMulti-Step Modewhen theMulti-Step Mode screen isshowing.Thisalsoholds true forcomputercontrolofthepumpthroughaserialconnection;thepumpmustbeswitchedtotheMulti-Step Modescreenonthepumpinterfacefirst.
Multi-Step Mode
Setup
Syringe Selection
In Multi-Step Mode, the left side of the screen shows a list ofselectableitems.Thefirst(topmost)iteminthislistisSetup. Tapping SetupwilltakeuserstotheMulti-Step Method Setup screen,wherethe syringe inner diameter (ID), total number of steps, number ofloops,andflow-rateunitscanbeset.
Syringe selection for Multi-Step ModeworksinmuchthesamemannerasseeninBasic Mode.
ChangesinthesyringeIDwillaffectthemin/maxvaluesfortheVolumefieldinallsteps.However,unlikeBasic Mode, importing asyringefromtheSyringeLibrarywillnotchangethevolumesineachsteptothevolumeofthesyringeunlesstheyexceedthevolumelimitsofthenewsyringe.Allvaluesineachstepthatareoutsidethesenewlimitswillbeadjustedtobewithinthelimits.
Multi-Step Mode Setup screen
Tip:Because changing the values of the syringe ID may possibly change the values and units for volume and rate, selecting a syringe or entering the syringe ID should always be the first step when setting up a run.
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Total Steps
Loop All
Sub-Loops
TheTotal Stepsfielddetermineshowmanystepswilloccurinthemethod.EachstephasindependentvaluesforVolume, Rate, Delay, and Infusion/Withdrawal.Uponenteringthenumberofsteps,thelistontheleftofthescreenwillupdatetoshowallofthesteps.Eachoneofthestepswillhavetheirownsetofparametersthatwillneedtobeset(seeStepParameters).
Aminimumof1andmaximumof99stepscanbeenteredasamethod.
AddingstepsisaccomplishedbyincreasingtheTotal Steps,whichwilladdthenewstepsattheendofthelist.DecreasingtheTotal Stepswillremovestepsfromtheendofthelist.Ineithercase,thevaluesintheStepParametersforeachexistingstepdoesnotchangeupontheadditionordeletionofsteps.
Inthecasethatthenumberofstepsinthelistontheleftsideofthescreenexceedstheavailablespaceonthescreen,abluetrianglearrowwillappearbelowthelistindicatingthatitcanscroll.Thislistcanscrolltwoways:(1)theusercandragtheirfingerupanddownonthelisttoscrollthelistandthentapontheitemofinterest;or(2)theusercanusetheup/downarrowsinthenavigationkeypadtoscrollthroughtheitems.
TheLoop Allfielddetermineshowmanyadditionaltimesthepumpwillperformallofthesteps.Thisisthehighestlevelloopandisthelastlooptoiterateafterallthestepsandsteploopshavecompleted.
Thisfunctionhasbeendeprecatedandisnotsupported.Please leave these fields empty.
TheLoop Allfieldtakesanyintegerfrom0to99.Avalueof0indicatesthatthemethodwillrunthroughtheentiretyofstepsonlyonce.Anyvalueabove0representsadditionaltimestorunallofthesteps.
Inthepumprunningscreen,thecurrentloopnumberandtotalnumberofloopsareindicatedinthebluebaratthetopofthescreen.
Example:In a multi-step method of three steps (1, 2, 3) where the Loop All is set to “2”. The method will run the following steps sequentially: 1, 2, 3, 1, 2, 3, 1, 2, 3. As shown, the method will loop through all three of the steps two additional times.
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Flow-Rate UnitsTappingtheVolume or Pump Ratebuttonopensascreenthatallowsflow-rateunitstobeset.Theflow-rateunitdetermineswhichunitofvolumeandtimewillbeusedfortheVolume and Ratefieldsforallofthesteps.ThissettinginMulti-StepModealsochangestheunitsforBasicMode.
Every step in themulti-stepmethod will have a selectableentryinthelist.Atthetopofthescreenyouwillfindaplacetoenteryoursyringvsizeaswellasthenumberofstepsthemulti-stepmethod to take. Tapping the stepwill allow youtochange theVolumeandRate.Thearrowson this screenrepresentinfuseorwithdrawforeachstep.
Important Note:Even if the units have changed, the values within the Volume and Rate fields will not change, which will result in significant changes in the flow rate and volume transferred. Additionally, the min/max limits for these fields will also change, so if the current values for volume and rate exceed the new limits, they will be adjusted to be within the limits.
Multi-Step Mode Options screen
Multi Step Program Screen
Step Parameters
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VolumeTheVolumefieldrepresentsthetotalvolumeofliquidthatthepumpwillattempttotransfer(infusionorwithdrawal)beforecompletingthestep.Theamountoftimethatthepumpwillattempttorunwiththesetvolumecanbedeterminedbydividingthevolumebytheflowrate(aslongastheflowrateisstatic).
Thevalueenteredcanbeanyintegerordecimalwithinthelimitsforthesyringe.Themin/maxvolumelimitscanbefoundinthebluebaratthetopofthescreenwhentheVolumefieldisselected.Anyenteredvaluebeyondtheselimitswillbeautomaticallyadjustedtotheminimumormaximumlimit,whicheveriscloser.Thetotalnumberofdecimalplacesislimitedtofive.
ThevolumecanbestatedinunitsofmLorμL.TheunitsusedforthevolumearedependentuponthesetunitsofvolumefortheflowrateintheOptionsscreenduringthemulti-stepsetup.
Tocontinuouslyrunthepumpandusetheentirevolumeofthesyringe,userscansetthevolumetothetotalvolumeofthesyringeortothemaximumlimit.However,usersarestronglyencouragedtosetthesafetycollar/bar(seeAdjustingtheSafetyCollar)sothatthepumpdoesnotpotentiallyruinthesyringe.
Important Note:Even if the units have changed, the values within the Volume and Rate fields will not change, which will result in significant changes in the flow rate and volume transferred. Additionally, the min/max limits for these fields will also change, so if the current values for volume and rate exceed the new limits, they will be adjusted to be within the limits.
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Thepump’sactionsarecontrolledbytheStop, Pause, and Start keysonthefront.Inordertostartarun,thescreenshouldbeshowingeithertheBasic Mode (for basic runs) or Multi-Step Mode(formulti-stepruns)mainscreens.PressingtheStartkeyineitherotherthesescreenswillstartarunwiththecurrentlysetparameters.
Amount Functions
Start
PressingtheStart buttononcewillstartanewrunwiththecurrentlydisplayedparametersorresumeapausedrun.ThisbuttonisonlyfunctionalwhenaruniscurrentlypausedorwhenthedisplayisshowingtheBasic Mode or Multi-Step Mode screensthatshowthecurrentparameters.
PressingtheStartbuttonwhileadelaycountdownisoccurringwillimmediatelyskiptheremainingdelaytimeandstarttherun/step.
PressingandholdingtheStartbuttonwillbeginpriming,wherethepumpwillcontinuouslyrunatthePrime/BolusRateaslongasthebuttonisbeingpressed.ThePrime/Bolus Rate is set in Basic Mode > Options. Priming is only available in Basic Mode.
ThegreenLEDlightontheStartbuttonwillbeonwhilethepumpisrunning.
Pause
Whilethepumpisrunning,pressingthePausebuttonwillpausethecurrentrun.Pausingallowsthepumptocontinuethesamerunfromthepointwhenthepauseoccurred.
TheyellowLEDlightonthePausebuttonwillbeonwhenthecurrentrunhasbeenpaused.
Stop
Duringarun,pressingtheStopbuttonwillimmediatelystoptherun,andreturnthedisplaytotheBasic Mode or Multi-Step Mode screen.
Whileoutsideofanactiverun,pressingtheStopbuttonwillexitthecurrentscreen.Thisresultisthesameasactivatingthe“X”buttoninthetoprightcornerofthescreenbytouchornavigationalkeypad.
TheredLEDlightontheStopbuttonwillbeonwheneverthepumpisneitheractivelyrunningnorpaused.Thelightwillalsobeonforactiverunsthatarecurrentlyinadelay before starting a step.
TheredLEDlightwillblinkifthepumphasstalled.
Uponstartingapumprun,thescreenwillswitchtotheSystem Statusscreen.TherearetwodifferentversionsofthisscreendependinguponwhetherthepumpwasstartedinBasic Mode or Multi-Step Mode.
Pump Control
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Run Status
The Run Status screen for Basic Modehighlightsthecurrentactivityofthepumpduringarun.
TheElapsed Timefieldindicateshowmuchtimehaspassedsincethepumphasstartedphysicallyrunning,notincludinganystart-time delay. The Infusion Timefieldshowsthecalculatedtotaltimeforthecurrentruntofinish,barringanypauseorstallintheinstrument.
Thetworectangularblocksonthescreenillustratethetransferofvolumeduringtherun.Theleftrectanglerepresentsthevolumeinthesyringewhiletherightrectanglerepresentsthevolumetransferredinoroutofthesyringe.Whilethepumpisrunning,asmallarrowwillappearbetweenthetworectanglesindicatingthedirectionofflow.Dependinguponthedirectionofthearrow,thenumberswithineachrectanglewithchangeasvolumeistransferred.
Thecoloredtextdirectlynexttothevolumerateamountindicatesthecurrentstatusofthepump:green-running;yellow-pausedordelayed;andred-stoppedorstalled.
The Volume,andRatefieldsindicatetheparametersthatweresetforthecurrentlyrunningmethodintheBasic Mode main screen.
TappingtheEdit Ratebuttonallowsuserstochangetheflowrateofthepumpinthemiddleoftherun.
Basic Mode System Status screen
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Start Options
Start Options
When starting the Fusion Series pump a display of startoptions will be shown. User will be required to select oneoptionbasedon thepump thatneeds tobestarted.Otherfunctionsarealsoavalibletopauseandstopthepumponcethepumphasstarted.
Pause Options
Stop Options
Start Options
Pause Options
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Thebaudrateistherateatwhichinformationistransferredthroughtheserialconnection.Thisrateisoftenlimitedbythetypeof serial connection used.
TheFusionSeriespumpsprovidetwopossiblebaudrates:9600and38400.
SerialconnectionsusingtheUSB-Bportonthebackofthepumpshouldusethefasterbaudrateof38400.
SerialconnectionsusingtheDB9portonthebackofthepumpwillneedtousetheslowerbaudrateof9600.
See Pump Control by Computer for more information.
Baud Rate
TheSystem SettingsscreencanbereachedbytappingontheMenuiconontheMode Selection screen.
System Settings
System Settings screen
Power Settings screen
TheSystem SettingsscreencanbereachedbytappingonthePowericonontheMode Selection screen.
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TheFusion4000andFusion6000pumpscangenerateuptoamaximumof65lbsand500lbsoflinearforce,respectively.Somesyringesmadeofmorefragilematerialsmaynotbeabletowithstandthefulllinearforceofthepump.OntheFusionSeriespumps,themotorpower,andthusthelinearforce,canbedecreasedintheSystem Settings screen.
Toadjustthissettingbytouch,tapthesectionofthepercentagetogglethatrepresentsthedesiredmotorpowerpercentage(1000%or50%).
TheLCDdisplaygoestosleepafter1hourafterthelastinteractionwiththepumpcontrols.Whenthedisplaygoestosleep,thepumpisstillon.Itwillcontinuetorunthecurrentmethodandisabletoreceivecommandsviacomputerconnection.
Motor Force
LCD Power-Off Time
Important Note:Even if the units have changed, the values within the Volume and Rate fields will not change, which will result in significant changes in the flow rate and volume transferred. Additionally, the min/max limits for these fields will also change, so if the current values for volume and rate exceed the new limits, they will be adjusted to be within the limits.
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Pump Control by ComputerTheFusionSeriessyringepumpscanbecontrolledwithanexternalcomputerusinganRS232serialconnection.
Twotypesofconnectionsareavailableforexternalcomputercontrol:DB9andUSB,cablesforeachareavailableforpurchasethroughChemyx(call1-281-277-5499orvisitwww.chemyx.com/contact-us).
Mostcomputeroperatingsystems (e.g.,Windows,macOS,Linux,etc.)havebuilt-in terminalprograms thatcanbeused totypeinthecommandsthatrunthepump.OnWindowsXPandearlier,HyperTerminalcanbeusedtocontroltothepump.OnmacOSandLinux,thescreencommandinTerminalcanalsobeusedtocontrolthepump.Therearealsonumerousthird-partyprogramsforserialconnectionsthatexistforeachoperatingsystemthatmaybeeasiertouseandprovidemorefeatures.Athird-partyprogramwillbenecessarytoconnectwithcomputersrunningWindowsVistaandlater.
For tipsonoperating thebuilt-in terminalprogramsand informationonavailable third-partyprograms,please visitwww.chemyx.com/support.
FortheDB9serialconnection,aMale-to-FemaleDB9cablemustbeused.Donotuseany“nullmodem,”“crossover”,or“crossed-over”cables.Thecablecanbeofanylengthupto50ft;however,thespeedoftheconnectionisoftendetrimentallyaffectedatlengthsover15ft.
ForaUSBserialconnection,aUSB-AtoUSB-Bcableshouldbeused.ThemaximumpossiblelengthofasingleUSBcableis15ft;however,USBcablesshouldbelessthan10ftforoptimumperformance.MultipleUSBcablescanbechainedtogethertomakealongerconnectionaslongasthehubconnectingeachindividualcableisconnectedtoapowersupply.
AUSB-to-DB9serialadaptercanbeusedtoconnectaDB9serialconnectiontotheUSBportonacomputer.
Alloftheseconnectionsrequiretheproperdriverstobeinstalledonthecomputer.Typically,mostcurrentoperatingsystemsalreadycontaindriversfortheUSBserialconnection.TheymayalsoalreadyhavedriversfortheDB9serialconnection.USB-to-DB9adapterstypicallyneedtohaveaUSB-to-serialdriverinstalled.Formoreinformationondownloadingandinstallingdriverspleasevisitwww.chemyx.com/support.
DB9 Serial Cable (Male-to-Female) USB-A to USB-B cable
Cable Requirements
Connecting
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Regardlessoftheoperatingsystembeingused,theterminalprogramcontrollingtheserialconnectionshouldusethefollowingconnection settings:
ThebaudratesettingfortheserialconnectionmustmatchthebaudratesetinthepumpsettingsGUI(SeeBaudRateinSystemSettings).
Generally,thepumpshouldbesettousethehigherbaudrate(38400)ifaUSB-onlyconnectionisused.However,ifaDB9-onlyorUSB-to-DB9connectionisused,thebaudratemustbesetto9600.
Typically,ifthebaudrateisnotsetcorrectly,therewillbegarbledornoresponsetoanycommands
COM Port Settings
Baud Rate 9600 or 38400
Data Bits 8
Parity None
Stop Bits 1
Flow Control None
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Regardlessoftheoperatingsystembeingused,theterminalprogramcontrollingtheserialconnectionshouldusethefollowingconnection settings:
Command Description
help Displays a list of available commands.
start Startsthepumpwiththecurrentlysetparameters.Theparametersthatwillrundependuponwhichmode(Basic Mode or Multi-Step Mode)thepumptouchscreenisshowing.
pause Pausesthecurrentpumprun.Theruncanberesumedfromthispointusingthe start com-mand.
stop Endsthecurrentrun.Theruncannotberesumedandcanonlyberestartedfromthebegin-ning.
set diameterX
Setstheinnerdiameter(ID)ofthesyringeinmillimeters.
ThissettingsimultaneouslyaffectsthesyringeIDusedinbothBasic Mode and Multi-Step Modesettings,whichwillalsoaffectthevolumeandrateparametersinbothmodes.
Xcanbeanintegerordecimal(uptothreedecimalplaces)from0.103mmto40.000mm.
Example set diameter 11.73
Returnsthediameter.
setunitsX
Setstheunitsusedfortherateofinfusion/withdrawal.Thisparameteralsosetstheunitsfortransfervolume,wherethetransfervolumeunitwillbethesameasthevolumeunitusedintherate.
ThissettingsimultaneouslychangestheunitsusedinbothBasic Mode and Multi-Step Mode settings.
Xcanbeanintegerfrom0to3.Eachintegerrepresentsaspecificrateunit.
0 = mL/min1=mL/hr2=μL/min3=μL/hr
Example set units 2
Returnstheintegercorrespondingtothesetrateunit.
Pump Commands
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Command Description
set volume X,X,X,...
Setsthetransfervolume.Thepumpwillrununtilthevolumeinfusedorwithdrawnequalsthisvolume.Thiscommandalsosetswhetherthepumpisinfusingorwithdrawing(Fusion200only)andthenumberofstepsusedinMulti-StepMode.
Xmustbeanintegerordecimal(uptofivedecimalplaces)withinthevolumelimitsforthesyringe(Seereadlimitparametercommand).Anegativesigncanbeaddedinfrontofanyvaluetoindicateaswitchtowithdrawalmode(Fusion200only).
For Basic Mode, only one value is required. Entering only one value will automatically switchthepumptoBasicMode.
Example set volume 25.0
TosetvolumesinMulti-StepMode,thepumpmustbemanuallyswitchedtoMulti-StepModeonthepump.Foreachstep,avaluemustbeentered,separatedbycommasandnospaces.Thenumberofstepswillbeautomaticallyadjustedtothenumberofvaluesentered(i.e.,enteringonlythreevalueswillchangethenumberofstepstothree).
Example set volume 3,-2,4
Returnsthetransfervolume,transferrate,andtransfertime(inBasicMode).
Returnsonlythetransfervolumes(inMulti-StepMode).
settimeX,X,X,...
Setsthetargettimeforapumprun.Thiswilladjustthetransferratesothattherunwilllastfortheinputtime.Thetargetvolumewillremainthesame.ThiscommandalsosetsthenumberofstepsusedinMulti-StepMode.
Xmustbeanintegerordecimal(uptofivedecimalplaces).Theresultingtransferratemustbewithintheratelimitsforthesyringe(Seereadlimitparametercommand).
For Basic Mode, only one value[ is required. Entering only one value will automatically switchthepumptoBasicMode.
Example set time 1.0
TosettargettimesinMulti-StepMode,thepumpmustbemanuallyswitchedtoMulti-StepModeonthepump.Foreachstep,avaluemustbeentered,separatedbycommasandnospaces.Thenumberofstepswillbeautomaticallyadjustedtothenumberofvaluesentered(i.e.,enteringonlythreevalueswillchangethenumberofstepstothree).
Example set time 1.0,0.5,1.3
Returns target time and rate (in Basic Mode).
Returns no values (in Multi-Step Mode).
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Command Description
setrateX,X,X,...
Setsthetransferrateforinfusion/withdrawal.
Xmustbeanintegerordecimal(uptofivedecimalplaces)withintheratelimitsforthesyringe (See read limit parameter command).
For Basic Mode, only one value is required.
Example set rate 1.0
TosetratesinMulti-StepMode,thepumpmustbemanuallyswitchedtoMulti-StepModeonthepump.Foreachstep,avaluemustbeentered,separatedbycommasandno spaces.
Example set rate 1.0,0.5,1.3
Ifthenumberofvaluesislessthanthenumberofsteps,theremainingstepswillgainthesameratevalueasthelastenteredvalue.Anyextravaluesbeyondthenumberofsteps will be truncated.
VariableratesmayalsobesetinMulti-StepMode.Rate1andRate2foreachstepcanbesetusingabackslash.Eachstepisstillseparatedbyacomma.
Example set rate 0.5/1.0,0.5/1.0,1.3
Returnstheflowrateandtransfertime(inBasicMode).
Returnstheflowrate1andflowrate2foreachstep(inMulti-StepMode).
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Command Description
setdelayX,X,X,...
Setsthestarttimedelay(inminutes)priortoeachstepofapumprun.Uponstartingtheastep,thepumpwillwaituntilthedelaytimeelapsesbeforestartingtotransfervolume.
Xmustbeanintegerordecimal(uptofivedecimalplaces)withintheratelimitsforthesyringe(Seereadlimitparametercommand).
For Basic Mode, only one value is required. Entering only one value will automatically switchthepumptoBasicMode.
Example set delay 1.0
TosetratesinMulti-StepMode,thepumpmustbemanuallyswitchedtoMulti-StepModeonthepump.ForeachstepofMulti-StepMode,avaluemustbeentered,separated by commas and no spaces.
Example set delay 1.0,0.5,1.3
Ifthenumberofvaluesislessthanthenumberofsteps,theremainingstepswillgainthesamedelayvalueasthelastenteredvalue.Anyextravaluesbeyondthenumberof steps will be truncated.
Returnsthedelaytime(inBasicMode).
Returnsthedelaytimeforeachstep(inMulti-StepMode).
read limit parameter
Returnsthemin/maxvaluesthatcanbesetfortransfervolumeandtransferrate.Thereturnedvaluesaredependentonthesyringeinnerdiameter(ID).Thetransfervolumelimitsaretypicallygreaterthanthemaximumvolumeofthesyringebeingused.
Returns: max rate, min rate, max volume, min volume
dispensed volume Returnsthetransferredvolumeforthecurrentrun/step.Ifnorunhasstarted,returnsthetransferredvolumeforthelastrun/step.
elapsed timeReturnstheelapsedtime(inminutes)forthecurrentrun/step.Ifnorunhasstarted,returnstheelapsedtimeforthelastrun/step.Elapsedtimedoesnotincludeanydelay time.
view parameter
Returnsthecurrentlysetparameters(BasicModeonly).
Returns:rateunits,syringeinnerdiameter,transferrate,primingrate,time(inwholenumberminutes),transfervolume,timedelay(inwholenumberminutes)
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Command Description
status
Returnsthecurrentstatusofthepump.Thereturnedintegercorrelatestothestatusofthepump.
0: Pump stopped1: Pump running2: Pump paused3: Pump delayed4: Pump stalled
Returns:integerthatrepresentsthecurrentstatusofthepump.
hexw2XXXXXXstart
Sets multiple parameters in one command (Basic Mode only).
Xvariablesinorder:units: see set units commandtransfermode:0-infusion,1-withdrawalsyringe ID: see set diameter commandtransfervolume:seesetvolumecommand;nonegativestransfer rate: see set rate commandstart time delay: see set delay command
ExampleThe following example shows the command using these parameters: units = mL/min; transfer mode = withdrawal; diameter = 23.05 mm; volume = 1.2 mL; rate = 3.0 mL/min; delay = 30 sec (0.5 min) hexw2 0 1 23.04 1.2 3.0 0.5
Astartcommandcanbeaddedafterthesixparameterstoimmediatelystarttherunwiththenewparameters.
Example hexw2 0 1 23.04 1.2 3.0 0.5 start
Allsixparametersdonotneedtobeentered;however,allparametersuptotheparameter(s)ofinterestmustbealsobeincluded.Theotherparametersnotenteredwillremainastheyarecurrentlyset.
Example hexw2 0 1 23.04 2.4
save setting Deprecated command.
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Command Description
setprimerateX
Setsthepriming/bolusrate(BasicModeonly).
Xmustbeanintegerordecimal(uptofivedecimalplaces)withintheratelimitsforthesyringe(Seereadlimitparametercommand).
Only one value is required.
Example set primerate 1.0
Returnsthepriming/bolusrate(inBasicMode).
restart Powersoffandthenautomaticallypowersonthepump.
Insomecases,dependingonthesoftwareusedtoconnecttothepump,eachcommandmayneedtobefollowedbyalinefeedcharactersuchas“\n”or“\r”toexecute.Ifacommanddoesnotappeartowork,tryadding“\r”or“\n”totheendoftheline.
Whenthepumpdoesnotrecognizeacommand,itwillreturntextsimilartothefollowing:
Exampleset units 1 \r
ExampleCommand not recognized-type in “help”and press enter to see a command list.
In addition to the manual control of the pumps through a terminal, Chemyx provides pump controller programs as anunsupportedopen-sourceapplicationswritteninPython,aLabVIEWpackage,andaMATLABpackage.Moreinformationonthesetoolscanbefoundonthewebsiteatwww.chemyx.com/support.
Chemyx Pump Controller Programs
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Pumplubricationisthemostimportantpartofregularpumpmaintenance.Properlubricationensureslongservicelife,smoothflowperformance,andprotectionagainstcorrosivelabfumes.Thepumpmaynotbeadequatelycleanedandlubricatedifthefollowingoccur:(1)grindingsoundsarecomingfromtheleadscrew;(2)theblockreleasebuttondecouplesundertheloadbeforethepumpstalls;or(3)thepumpstallswithincreasedfrequency.
Please contact Chemyx (call +1 281-277-5499 or visit www.chemyx.com/contact-us) to confirm the safe use of a differentlubricant.
1. Cleancontactsurfaceswithacleancloth.Removeanydebrisontheleadscrewandtheguiderods.
2. Applylubricantalongthelengthoftheleadscrewandtheguiderods.
3. Presstheblock-releasebuttonandslidethepusherblocktotheleftedge.
4. Runthepumpend-to-endwithoutasyringetoworkthelubricantintothecomponents.
Pumplubricationshouldbeperformedeverythreemonths.
Pump Maintenance
• Engine/motor oil
• Silicone oil
• Machineoil
• Toolingoilor“WD-40”
• Lithium-basedgrease
Lubricating the Pump
Lubrication Instructions
Approved Lubricants
50
TheChemyxFootswitchprovidesuserstheabilitytostart,pause,orstopthepumpfromashortdistancewiththetapofafoot.
TheChemyx4-ChannelSyringeRackfortheFusion6000providesuserswithanexpansionofthenumberofsyringesthatcanbeusedsimultaneously.The4channelsyringerackcanaccommodateupto4syringeswithvolumesfrom 10 mL to 100 mL.
TheChemyx Footswitch for Fusion Series pumpshas aUSB-B cable thatplugsintotheTTLportonthebackofthepump.
TousetheFootswitch,thepumpmustbeineithertheBasicModeorMulti-StepModescreens.ThebasiccommandsaregivenbyacertainnumberofclicksoftheFootswitch.
AsingleclickoftheFootswitchwillstartthepumprunorpausethepumprundependingonwhetherthepumpiscurrentlyrunning.
AquickdoubleclickoftheFootswitchwillcompletelystopthepumprun.
Footswitch
Footswitch
Using the Footswitch
Pump Accessories
Footswitch
4-ChannelSyringeRack
4-Channel Syringe Rack (Fusion 6000 Only)
51
Attaching the Rack
Fusion 200
SyringeHolderwith10Channels
Flange Retaining Bracket
Clamp Extensions
Clamp Extensions
Plunger Retaining Bracket
Syringe Clamp
52
Size Diameter
6.00 mL 9.53mm
20.00 mL 19.13 mm
50.00mL 28.6 mm
Size Diameter
0.50mL 4.64 mm
1.00 mL 4.64 mm
2.00 mL 8.93 mm
2.50mL 8.66 mm
3.00 mL 8.93 mm
5.00mL 11.73 mm
10.00 mL 14.68 mm
20.00 mL 19.60 mm
30.00 mL 22.70 mm
50.00mL 28.03 mm
60.00 mL 28.60 mm
Size Diameter
0.50μL 0.10 mm
1.00μL 0.15mm
2.00μL 0.21 mm
5.00μL 0.33 mm
10.00μL 0.48 mm
25.00μL 0.73 mm
50.00μL 1.03 mm
100.00μL 1.46 mm
250.00μL 2.30 mm
500.00μL 3.26 mm
1.00 mL 4.61 mm
2.50mL 7.28 mm
5.00mL 10.30 mm
10.00 mL 14.57mm
25.00mL 23.03 mm
50.00mL 32.57mm
Size Diameter
1.00 mL 4.64 mm
3.00 mL 4.64 mm
5.00mL 12.07 mm
10.00 mL 14.50mm
20.00 mL 19.13 mm
30.00 mL 21.69 mm
60.00 mL 26.72 mm
Size Diameter
1.00 mL 4.80 mm
1.00 mL 6.70 mm
2.00 mL 6.70 mm
2.00 mL 9.20 mm
3.00 mL 10.30 mm
5.00mL 12.20 mm
10.00 mL 15.00mm
20.00 mL 19.00 mm
30.00 mL 22.50mm
50.00mL 25.50mm
100.00 mL 34.00 mm
Chemyx Stainless Steel Hamilton Glass
Hoshi
BD Glass
BD Plastic
AppendicesAppendix A: Syringe Volume/Diameter Reference
53
Size Diameter
1.00 mL 4.66 mm
2.00 mL 6.90 mm
2.50mL 9.10 mm
5.00mL 12.62 mm
10.00 mL 14.34 mm
20.00 mL 19.68 mm
30.00 mL 22.44 mm
50.00mL 28.80 mm
100.00 mL 36.68 mm
Size Diameter
0.25mL 3.45mm
0.50mL 3.45mm
1.00 mL 4.50mm
2.00 mL 8.92 mm
3.00 mL 8.99 mm
5.00mL 11.70 mm
10.00 mL 14.70 mm
20.00 mL 19.58mm
30.00 mL 22.70 mm
50.00mL 29.00 mm
Size Diameter
0.25mL 2.60 mm
0.50mL 3.20 mm
1.00 mL 4.30 mm
2.00 mL 6.30 mm
3.00 mL 7.30 mm
5.00mL 9.50mm
Size Diameter
2.00 mL 9.12 mm
5.00mL 12.34 mm
10.00 mL 14.55mm
20.00 mL 19.86 mm
30.00 mL 23.20 mm
50.00mL 27.60 mm
Size Diameter
1.00 mL 4.71 mm
3.00 mL 9.50mm
5.00mL 12.91 mm
10.00 mL 15.40mm
20.00 mL 19.30 mm
30.00 mL 23.40 mm
50.00mL 29.00 mm
Size Diameter
1.00 mL 4.70 mm
2.50mL 9.70 mm
5.00mL 12.48 mm
10.00 mL 15.89mm
20.00 mL 20.00 mm
30.00 mL 22.50mm
50.00mL 28.90 mm
JMC Air-Tite Plastic Norm-Ject Plastic
Popper & Sons Glass
Ranfac
Natsume
Nipro
54
Size Diameter
25.00μL 0.73 mm
50.00μL 1.03 mm
100.00μL 1.46 mm
250.00μL 2.30 mm
500.00μL 3.26 mm
1.00 mL 4.61 mm
2.50mL 7.28 mm
5.00mL 10.30 mm
10.00 mL 14.57mm
Size Diameter
1.00 mL 4.70 mm
2.00 mL 6.40 mm
3.00 mL 9.30 mm
6.00 mL 13.10 mm
12.00 mL 15.40mm
25.00mL 21.00 mm
30.00 mL 23.00 mm
50.00mL 29.00 mm
Size Diameter
1.00 mL 4.65mm
3.00 mL 8.94 mm
6.00 mL 12.70 mm
12.00 mL 15.90mm
20.00 mL 20.40 mm
35.00mL 23.80 mm
50.00mL 26.60 mm
Size Diameter
1.00 mL 4.73 mm
3.00 mL 9.00 mm
5.00mL 13.04 mm
10.00 mL 15.79mm
20.00 mL 20.18 mm
30.00 mL 23.36 mm
60.00 mL 29.45mm
Size Diameter
1.00 mL 4.73 mm
1.00 mL 6.50mm
3.00 mL 8.65mm
5.00mL 13.00 mm
10.00 mL 15.80mm
20.00 mL 20.15mm
30.00 mL 23.10 mm
50.00mL 29.10 mm
SGE Glass Terumo Japan
TopSherwood Plastic
Terumo
55
Size Diameter
10.00μL 0.46 mm
25.00μL 0.73 mm
50.00μL 1.03 mm
100.00μL 1.46 mm
250.00μL 2.30 mm
500.00μL 3.26 mm
1000.00μL 4.61 mm
Size Diameter
1.00 mL 4.65mm
3.00 mL 8.94 mm
6.00 mL 12.70 mm
12.00 mL 15.90mm
20.00 mL 20.40 mm
35.00mL 23.80 mm
60.00 mL 26.60 mm
Unimetrics
Kendall Monoject Plastic
56
Item Description Part Number
Fusion100Touch 07100
Fusion200Touch 07200
Fusion400Touch 07400
Item Description Part Number
Footswitch(w/USB-Bconnectioncable) 50002
DB9 Serial Cable (Male-to-Female) 50007
USB Serial Cable (USB-B-to-USB-A) 50017
10-ChannelSyringeRack(Fusion200only) 50003
Item Description Part Number
Stainless-Steel Syringe (6 mL) SS6
Stainless-Steel Syringe (20 mL) SS20
Stainless-SteelSyringe(50mL) SS50
O-Ring Replacement 10-pack (6 mL) CP6
O-Ring Replacement 10-pack (20 mL) CP20
O-RingReplacement10-pack(50mL) CP50
Fusion Series Syringe Pumps
Options and Accessories
Stainless-Steel Syringes
Appendix B: Available Parts
57
Chemyx Inc.
10905CashRd.
Stafford,TX77477
Webpage:www.chemyx.com
ContactUs:www.chemyx.com/contact-us
Phone:+1281-277-5499
Fax:+1281-277-5499
OfficeHours:Monday–Friday,8:30am–5pm(CentralTime)
Formorearticlesandtutorialvideos,visit:www.chemyx.com/support
Follow Us
LinkedIn:www.linkedin.com/company/chemyx
Twitter:www.twitter.com/chemyxinc
Appendix C: Contact Information
