GAPDHa Suppresses Heat Shock Induced DNA Fragmentation in Arabidopsis plant

No trea

ted

44 C

2h

44 C

2h+22

C 2

4h

No trea

ted

BIP:G

FP

GAPa:GFP

44 C 2h + 22 C 24h

A B

GAPDHa Suppresses Heat Shock InducedCell Death in Arabidopsis Plant

DA

PI s

tain

ed

cell

(%)

BIP:GFP GAPa:GFP

untreated

treated

32

0 32 64 96 128 160 192 224

64

- hea

t shock

+ hea

t shock

Co

un

ts

Heat Shock Induces ROS Accumulation in the Arabidopsis Plant

Heat Shock Induces ROS Accumulation in the Arabidopsis Plant

GAPDHa Suppresses ROS AccumulationInduced by Heat Shock stress

un

tre

ate

d

tre

ate

d

Bright Rh123

E

Nucleoside diphosphate kinase (NDPK)- a hexameric protein composed of 17kDa monomer

- catalyze the reversible transfer of the 5’-terminal phosphates fromtriphosphate nucleosides (or their deoxy derivatives) to nucleoside diphosphates to generate NTPs or dNTPs

- in animals, NDPKs have important role in control of cell proliferation, regulation of transcription, and protein phosphotransferase activity

- in plants, it is associated with phytochrome B response, UV-B signaling, heat stress and hormone related responses

- very little is known about the nature of regulation of NDPK gene in eukaryotes.

YNK is induced by oxidative stress

O OHHO

H

COOH

ClCl

DCFH-DA

DCFH

DCFfluorescence

Measurement of Redox-State in the Cell UsingDichlorofluorescein Diacetate (DCFH-DA)

Cell

H2O2OHO O

COOH

Cl Cl

H

O-C-CH3

COOH

H3C-C-O

Cl

OOO

Cl

% DCF stain F P Lw.t. 7.0 3.5 89.9ynk 10.7 36.0 53.3

w.t.

ynk

YNK Regulates ROS Generation

FL1, Rhodamin123 ; FL2, Propidium iodide

YNK Regulates ROS Generation

w. t. Bax ynk1