GAS CHROMATOGRAPHY

Mr. Maywan Hariono

One of chromatography technique that use a gas as a carrier (mobile phase)

The separation is based on the volatility of sample

Same with HPLC, GC is one of advance chromatography method and use Rt as a separation parameter

Phase??

Stationary phase: octadecyl silane, octasilane, aminosilane

Mobile phase (gas) : He, N2, H2, Ar

The principle and instrumentation of GC

Principles

The volatile sample in the liquid matter is injected to the column.

The temperature of column is setted in which sample can be vaporized.

The gas is pumped and then it carries the sample (gas) through the column.

The sample which has higher boiling point/melting point will be retained by column, in contrast the lower one will be carried through the column faster.

Means the higher b.p/m.p wil be lower the Rt and vice versa

Principles

The information from GC machine will be translated to the graph by software

Komponen2 GC

1. syringe2. Injection port3. column4. GC oven5. detector6. Carrier gas7. recorder

GC Component

a. Syringe 5-10 microliter in volume

GC Component

b. Injection port

GC Component

c. Column

Factors influence the GC separation performance

i.Boiling point/melting pointii. Column temperatureiii. Column lengthiv. The speed of gas streamv. Internal diameter of column

GC APPLICATIONS

1. Pharmaceutical industry2. Herbal medicine industry3. Environmental analysis4. Cosmetics analysis

adavntages

i. Precise and accurateii. Sensitive

Disadvantages GC

i. Time consumingii. Only volatile sample can be

determined using GCiii. The sample is damaged after

running, can not be recollected.

Rujukan

1.David G Watson(2005). Pharmaceutical Analysis. Second edition. Pp: 236-265

2.http://www.en.wikipedia.org/wiki/Gas-chromatography.html

3.http://orgchem.colorado.edu/hndbksupport/GC.html