Gene doping of mice with PGC1a via ormosil to

produce fitness

Kamran Rowshandel

The question

• Can exercise be substituted?

PGC1a

• Causes mitochondrial biogenesis

• Prevents ROS-mediated degradation and other damage to mitochondria by signaling for the expression of a slew of proteins that keep mitochondria healthy

• Expressed every time you exercise!

Ormosil

• Ormosil is organically modified silica and has many purposes due to physical characteristics it possesses due to the way it is on a “nano” level

• There are multiple ways of getting a gene to be expressed by an animal, but some methods have drawbacks

• Ormosil does not have drawbacks compared to viral gene delivery vectors, which have killed people in the past

The method

• Ormosil vectors are made by mixing the gene and ormosil together in solution and letting sit for 30 minutes

• IV injection into adult mice yields expression of the vector

Naturally,

• A group of 60 adult mice will be injected with the treatment

• A group of 40 adult mice will exercise daily on motorized treadmills

• And a group of 20 adult mice will be sedentary

Around the world in 80 days

• Groups will be examined for indicators of fitness such as muscle development by visual appearance on a daily basis as they are fed and taken care of

• On the final day all mice will be run on motorized treadmills to exhaustion

Just maybe

• Perhaps the group which received treatment will be able to run as long as the mice which exercised daily

• Examination of the muscle tissues in each group by microscope will then be done to find what changes high levels of PGC1a can cause in muscle tissue