Date post: | 04-Jan-2016 |
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Welcome to Lab!
Be sure to familiarize yourself with safety procedures
○ Where does broken glass go?○ What goes in the biohazard?○ Etc.
Be sure to wear closed-toed shoes to lab everytime!
Properly reading Graduated Cylinder Always use graduated cylinder to
measure volumeDo not use a beaker!
Metric System
Used in measurements of:Length – MeterWeight – GramVolume – LiterTemperature – Celsius°C = (°F – 32°)/1.8
Important Equations to Know
Area of a block = Length x width
Volume of a block = Length x width x height○ 1cm3 (cc) = 1ml
Density = Mass/Volume
Displacement volume =Total volume (after object) – Original volume (before
object)
Temperature conversions:°C = (°F – 32°)/1.8
Practice Problems
1. 51 cm = ______mm
2. 92 cm and 1 mm = ______mm
3. 72 mm = _____cm
4. 317 m = _____cm
5. 1.35 km = _____m
6. 4156 cm = _____m
7. 8000 m = ______km
8. 83 g = _____mg
1. 976 g = _____mg
2. 4000 mg = _____g
3. 3000 g = _____kg
4. 8 L = _____ml
5. 1.75 L = _____ml
6. 985 ml = _____L
7. 7428 ml = _____L
Practice Problem Answers1. 510 mm
2. 921 mm
3. 7.2 cm
4. 31700 cm
5. 1350 m
6. 41.56 m
7. 8 km
8. 83000 mg
1. 976,000 mg
2. 4 g
3. 3 kg
4. 8000 ml
5. 1750 ml
6. 0.985 L
7. 7.428 L
Microscopy Light Microscope
Binocular dissecting microscopeCompound light microscope
Electron MicroscopeTransmission Electron Microscope
○ Thin slices of specimen
Scanning Electron Microscope○ Image of surface of specimen
The Objective Lenses Scanning Objective (4x)
Red color band, smallest one Low-power objective (10x)
Yellow color band High-power objective (40x)
Blue color band Oil Immersion objective (100x)
Gray color bandOnly use with oil! Have to carefully clean
oil off afterwards!!!!!
Magnification
Magnification = ocular x objectiveOcular lense is usually 10
Examples:○ Under 4x objective – magnification of specimen
is 40 x○ Under 10x objective – magnification of
specimen is 100x○ Under 40x objective – magnification of
specimen is 400x
Microscopy Using the Microscope
Find the microscope # assigned to you in the cabinet Carry the microscope with BOTH hands back to the lab bench Plug it in, turn on light Place slide on stage Always view specimen on lowest power first!
○ Focus and center Switch to higher power
○ Focus and center, etc. When done:
Switch lens to lowest power!! Take slide off Clean lens with only lens paper Turn light off Unplug wrap cord around base Place back in cabinet
Things to Remember
Be careful with Microscope Never turn head around! Always clean lenses with lense
paperIn your drawer
You can clean slides with Kimwipes Always put slides back in slide box
in numerical order!!!!!