Good afternoon, Agents.

Please take out a writing utensil and place your backpack below your

desk.

Crime Scene Investigation: Macromolecule Identification

Period 4 Debriefing Meeting

Crime scene #UNI091811

At 3:15 PM on Friday, September 16th 2011, an iPod belonging to Jane Doe was stolen.

Remnants of food found at the scene have been taken as evidence. Four suspects have been questioned, and food items from their lockers confiscated.

LIBRARY 9/18/2011

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CASE #UNI091811

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CASE #UNI091811

Sketch by crime artist

Your task is to identify the food item in the evidence based on your biochemistry knowledge of macromolecules.

So what do you know about macromolecules?

CARBOHYDRATES

PROTEIN NUCLEIC ACID

LIPID

monosaccharide

amino acid

fatty acid

nucleotide

polysaccharide

Each team will be given 3 types of samples: 1.Dry evidence2.Liquid evidence3.One food sample from the

suspects:SUSPECT FOOD SAMPLE CONFISCATED

A Strawberry jam cookiesB Buttered breadC PretzelsD Bean burrito

TEAM NAME FOOD SAMPLE

Team Red Strawberry jamTeam Orange Cookies

Team Yellow Butter

Team Green Bread

Team Blue PretzelsTeam Purple Beans

You have been assembled into color-coded teams.

The results of each team’s suspect food data will be compiled at 1:15 PM with our second debriefing meeting.

From this meeting, you will be able to identify your unknown evidence and single out the culprit.

We will take the time now to assign a number 1-6 to each

agent in your team.Agent #1: Polysaccharide expertAgent #2: Monosaccharide expertAgent #3: Protein expertAgent #4: Lipid expertAgent #5: RecorderAgent #6: Recorder

Agent #1: Polysaccharide expert

You will use Iodide to determine the presence of starch.

BASIC PROCEDURE1.Put on lab apron, gloves, and

goggles.2.Label test tubes3.Pour out dry evidence into weigh

boat. Use back end of spoon to scoop and pour evidence.

4.Add water to the line, tap to mix well.

5.Add biochemical indicator, tap to mix well.

6.Relay observation to recorder.

Agent #1: Polysaccharide expert

Add 5 drops of Iodide to determine the presence of starch.

Iodide solution changes from

clear orange to deep blue-black in presence of

starch

Agent #2: Monosaccharide expert

Add 8 drops of Benedict’s solution to determine the presence of glucose.

Benedict’s solution changes from clear

blue to thick yellow-orange-red in presence of

glucose

Agent #3: Protein expert

Add 8 drops of Biuret solution to determine the presence of protein.

Biuret’s solution changes from clear

light blue to lavender-brown in

presence of protein

Agent #4: Lipid Expert

Smear the liquid sample onto a paper bag to determine the presence of lipids.Only you will be responsible for the LIQUID evidence.

You will have until 1:27 PM to complete this investigation.

Good luck teams.

CASE #UNI091811

• Return unused dry evidence back into tube

• Place dirty test tubes into water tub—don’t worry about gunk!

• Throw away lipid brown papers

• Return racks, water bottles into shoe box.

Actual Samples and EvidencePolysaccharide test Monosaccharide test

Protein test

Thank you scientists!TEAM NAME CULPRIT EVIDENCE

Team Red C PretzelsTeam Orange D Bean burrito

Team Yellow A Strawberry jam

cookiesTeam Green C Pretzels

Team Blue D Bean burrito

Team Purple B Buttered bread