Fermentation: Kinetics of Yeast GrowthAdam ClarkCorey HicksonGroup ENovember 25th, 2013OutlineIntroductionApparatus and ProcedureData Processing and AnalysisResults and DiscussionReferences

IntroductionTheoryWhat is fermentation?Growth of an organism: lag, acceleration, and de-accelerationObjectiveTo study the biological process of yeast growth and to determine the kinetics of the process

Figure 1: Graph of the organism growthFermentation is the growth of microorganisms on an organic substrate Lab ManualThere can also be respiration, which involves air, but well be looking at an actual fermentation process, ie. Low air, causing it to take the fermentation pathway.The lag phase is the organism settling into its new conditions. Little cell mass is generated. 3Apparatus and ProcedureApparatusOmni Culture Plus, RM 20, UVmini 1240, YSI Select, Universal 32 R, GC-2014 Shimadzu Gas ChromatographProcedurePreparation: inoculate the yeastTake samples after the 3 hour lag phase for absorbance, ethanol, and glucose.View a sample under a microscope to see the yeast

Figure 2: diagram of the apparatusData processing and analysisInitial Conditions:90 g/L Glucose4.5 g/L Yeast33.7 0CdX/dt = X, ln(X/X0)= mtMonod kineticsProduct and cell yieldsAcquiring Ks

Figure 3: Graph of the calibration curveTo get Ks, we need to play S vs (t/(1+Ket)*S1 and the intercept will be Ks/mumax

Ks is around 180 for glucose.5Results and DiscussionFrom our calculations, we determined:Yp,ave = Product Yield = 0.264Yx,ave = Cell Yield = 0.13m= 0.156 hr -1

Talk about what the yields mean in layman terms6Design Project Results:Total working hours of 8400 hours per yearTotal batch production time (including downtime) of 21.76 hours386 Batches per year9170 kg of Ethanol produced per year

Talk about assumptions and given details7Yeast Cells

Conclusions and Recommendationsm= 0.156 hr -1386 batches per year with a production of 9170 kg of ethanol produced per yearMonod focuses on the limiting nutrient, S, however growth may not entirely depend on S (ie. Other factors)http://www.rpi.edu/dept/chem-eng/Biotech-Environ/FERMENT/roth1.htmlTalk about recommendations9ReferencesRoth, Charles. "Semester project for Introduction to Biochemical Engineering."Chuck's Biochem work. N.p., n.d. Web. 5 Nov 2013. .Thanks for listening!