Hellenic Accreditation System
Annex F1/B15 to the Certificate No. 44-4
SCOPE of ACCREDITATIONof the
Analytical Laboratoryofthe
AGROLAB SA. (Laboratory in Athens)
Materials / ProductsTested
Types of Test / Properties Applied Standards /Techniques
Chemical Testing
1. Fruits andVegetables highwater content
(stone fruits, pomefruits, fruitingvegetables,citrusfruits, root-tubervegetables, stemvegetables, smallfruits, pulsesvegetables, brassicavegetables, bulbvegetables, leafvegetables and freshherbs,miscellaneous(including tropicfruits, as referred toRegulations(EQ396/2005 and(EQ187/2006)
1. Determination of residues of!74 pesticides:Acephate, Acetamiprid, Aldicarb sulfone, Azamethiphos, Azinphos methyl,Azoxystrobin, Benomyl, Boscalid, Brornuconazole, Buprimate, Buprofezin*,Butocarboxim sulfone, Butocarboxim sulfoxide, Carbaryl, Carbendazim, Carbofuran,Carbofuran 3hydroxy, Carpropamid, Chlorbomuron, Chlorfenvinphos cis,Chlorfenvinphos trans, Chlorpyrifos, Chlorpyrifos-methyl, Clofentenzine, Clotdianidin,Cyazofamid, Cymoxanil, Cyproconazole(*), Cyprodinil (*), Demeton-O-sulfoxide,Demeton-S- methyl sulfone, Desmedipham, Diazinon, Dichlofluanid, DMSA (degr.dichlofluanid), Dichlorvos, Dicrotophos, Diethofencarb, Difenoconazole, Diflubenzuron,Dimethoate, Dimethomorph, Dimoxystrobin, Diniconazole{*), Diuron, Dodemorph,Epoxiconazole, Ethiofencarb sulfone, Ethiofencarb sulfoxide, Ethion, Ethiprole,Etofenprox, Famoxadone, Fenamiphos, Fenarimol, Fenazaquin, Fenbuconazole,Fenhexamid, Fenoxycarb, Fenpropimorph, Fenpyroximate, Fenthion, Fenthion sulfoxide,Fludioxonil, Flufenacet, Flufenoxuron, Fluquinconajale, Flusilazole, Flutolanil, Flutriafol(*), Fosthiazate, Furalhiocarb, Halofenozide, Hexaconazole, Hexailumuron, Hexythiazox,Imazalil, Imidacloprid, Indoxacarb, Iprovalicarb, Isoprothiolane, Isoxaflutole, Isoxathion,Kresoxim-methyl, Linuron, Lufenuron, Malathion, Mefenacet, Mephosfolan, Mepronil,Metalaxyl, Metconazoie, Methamidophos, Methidathion, Methiocarb, Methiocarbsulfone, Methiocarb sulfoxide, Methomyl, Methoxyfenozide, Metobromuron, Metoxuron,Monocrotophos, Monolinuron, Myclobutanil, Nitenpyram, Nuarimol, Omethoate,OxadLxyl, Oxamy! (*), Oxycarboxin, Oxydemeton-methyl, Paclobutrazole, Penconazole,Pencycuron, Phenmedipham, Phorate sulfoxide, Phosalone, Phosphamidon, Phosalone,Picoxystrobin, Piperonyl butoxide, Pirimicarb, Pirimicarb desmethyl, Pirimiphos-methyl,Prochloraz, Profenofos, Propargite, Propamocarb, Propiconazole, Propoxur,Propyzamide, Pymetrozine (*), Pyraclostrobin, Pyrazophos, Pyridaben, Pyridaphenthion,Pyridate degradation, Pyrifenox, Pyriinethanil, Pyriproxyfen, Quinoxyfen .Spinosad A,Spinosad D, Spirodiclofen, Spiroxamine, Spiromesifen, Tebuconazole, Tebufenozide,Tebufenpyrad, Tetraconazole, Thiabendazole, Thiacloprid, Thiamethoxam, Thiodicarb,Thiophanate methyl, Thiofanox sulfone, Thiofanox sulfoxide, Thiometon sulfone,Thiometon sulfoxide, Tolclophos-methyl, Tolylfluanid, DMST (degr. tolylfluanid),Triadimephone (*), Triadimenol (*), Triazophos, Trichlorphon, Tricyclazole,Trifloxystrobin, Triflumizole, Triforine, Tritoconazole(*), Vamidothion sulfone,Vamidothion sulfoxide, Zoxamide
* Accredited only for Grapes
Modified method UPLC-MS-MSbased on:
1. Lehotay el aL AOAC Vol.88,No.2,2005 (Modified), 615-629
2. SANCO/12495/2011 oftheEuropean Commission
Page 1 to 8 Annex F1/B15 to the Certificate ESYD No 44-4 21.10.2013
Types of Test / Properties Applied Standards /Techniques
2. Grapes I . Determination of 116 pesticide residues
Aclonifen, Alachlor, Aldrin, Ame&yn, Azinphos-ethyl, a-HCH, b-HCH, Bifenthrin,Bromacil, Bromophos-ethyl, Bromophos-methyl, Brompropylate, Butafenacil,Cadusaphos, Carbophenothion-methyl, Chlorbenzilate, [Chlorfenson 1 + Chlorfenson 2],Chloroneb, Chlorthai-dimethyl, Chlozolitiate, Clethodim, Clomazon, Cyanofenphos,[Cyfluthrin 1 + Cyfluthrin 2+ Cyfluthrin 3+ Cyfluthrin 4], [Cypermethrin 1 +Cypermethrin 2+3+ Cypermethrin4], d-HCH, Dichlobenil, Dichiofenthion, Dicloran,Dieldrin, Diflufenican, Diphenylamine, Dipropethryn, Disulfoton, Endosulfan alpha,Endosulfan beta, Endosulfan sulfat, Endrin, EPN, Ethoprophos, Ethridiazole,Ethofenprox, Fenchlorphos, Fenitrothion, Fenpropathrin, Fenson, Fensulfothion,Fipronil, [Flucythrinate 1+ Flucythrinate 2), Fluotrimazol, Fonofos, Heptachlor,Heptachlor epoxide, Heptenophos, lodofenphos, Iprobenfos, Isodrin, Isofenphos,Isofenphos-methyl, Isazofos, lambda-Cyhalothrin, Leptophos, Lindane (g-HCH),Mecarbam, Mefenpyr-diethyl, Methacrifos, Methoxychlor, Metolachlor, Mirex,Nitrapyrin, Nitrothai isopropyl, o,p-DDD, o,p-DDE, o,p-DDT, o-Phenylphenol,Oxadiazon, p,p-DDD, p,p-DDE, p,p-DDT, Paraoxon-ethyl, Parathion-ethyl, Parathion-methyl, Pebulate, Pentachloraniline, Pentachloranisole, [Pennethrin 1+ Permethrin 2],Phenthoate, Primiphos-ethyl, Procymidone, Propachlor, Propanil, Propetamphos,Propham, Prothiophos, Pyrimicarb desmethyl, Quinalphos, Quinoxyfen, Quintozene, S-421, Sulprofos, Tecnazena Tefluthrin, Terbacil, Tetrachlorvinphos, Tetradifon,Tetramelhrin, Tetrasul, Transfluthrin. Triadimefon, Trichloronate, Vindozolm
Modified method GC-MS-MSbased on
1. Lehotay el ai: AOAC Vol.88,No.2, 2005
2. SANCO/12495/2011 of theEuropean Commission
3. Fruits and Vegetableshigh water content
(stone fruits, pomefruits, fruitingvegetables,citrusfruits, root-tubervegetables, stemvegetables, smallfruits, pulsesvegetables, brassicavegetables, bulbvegetables, leafvegetables and freshherbs,miscellaneous(including tropicfruits, as referred toRegulations(EQ396/2005 and(EC) 187/2006)
1. Determination of Dithiocarbamate pesticide residuesModified method GC-PFPD-S,based on
1. ANDRE DE KOK ET ALL, 6™EUROPEAN PESTICIDERESIDUE WORKSHOP, (2006)
2. SANCO/12495/20II of theEuropean Commission
4. Oil I. Determination of 13 organochloride pesticide residues
[diazinon, dimethoate, chlorpyriplios, fenthion, fenthion oxon, malathion,parathion, methidathion, fenthion sulphoxide, fenthion sulfone]
Modified method based on
A. Journal of AOAC International77(5). 1096(1994)B. DFG Manual of PesticideResidues Analysis METHOD S-9
Page 2 to 8 Annex F1/BI5 to the Certificate ESYD No 44-4 21.10.2013
Materials/Productstested
Types of Test / Properties Applied Standards/Techniques
Oil (continue) 2. Determination of 136 pesticide residues
methyl, Azoxystrobin, Bitettanol, Boscalid, Brornuconazole, Buprimate, Buprofezin,Butocarboxim sulfone, Butocarboxim sutfoxide, Carbaryl, Carbofuran, Carbofuran3hydroxy, Carpropamid, Chlorbrornuron, Chlortenvinphos cis, Chlorfenvinphos trans,Chlorpyrifos-methy], Clotdianidin, Cyazofamid, Cymoxanil, Cyproconazole,Cyprodinil, Demeton-O-sulfoxide, Demeton-S- methyl sulfone, Desmedipham,Diazinon, Dicrotophos, DMSA (degr. dichlofluanid), Diethofencarb, Difenoconazoie,Diflubenzuron, Dirnethoate, Dimethomorph, Dimoxystrobin, Diniconazole, Diuron,Epoxiconazole, Ethiofencarb sulfone, Ethiofencarb sulfoxide, Ethion, Ethiprole,Famoxadone, Fenamidone, Fenamiphos, Fenarimol, Fenbuconazole, Fenoxycarb,Fenthion, Fenthion sulfoxide, Fludioxonil, Flufenacet, Flusilazole, Flutolanil,Flutriafol, Fosalone, Fosthiazate, Furathiocarb, Halofenozide, Hexaconazole,Hexythiazox, Imidacloprid, Indoxacarb, Iprovalicarb, Isoprothiolane, Isoxaflutol,Isoxathion, Kresoxim-methyl, Linuron, Malathion, Mefenacet, Mepanipyrim,Mephosfolan, MeproniK Metalaxy!, Metconazole, Methamidophos, Methidathion,Methiocarb, Methiocarb sulfoxide, Methomyl, Methoxyfenozide, Metoxuron,Monocrotophos, Monolinuron, Myclobutanil, Niiarimol, Omethoate, Oxadixyl,Oxamyl, Oxamy! oxime, Oxycarboxin, Paclobutrazole, Penconazole, Pencycuron,Phenmedipham, Phorate sulfoxide, Phosphamidon, Picoxystrobin, Piperonyl butoxide,Pirimicarb, Pirimicarb desmethyl, Pirimiphos-methyl, Prochloraz, Profenofos,Propiconazole, Propoxur, Propyzamide, Pyraclostrobin, Pyrazophos, Pyridaphenthion,Pyrimethanil, Spiromesifen, Tebuconazole, Tebufenpyrad, Tebufenozide,Tetraconazole, Thiacloprid, Thiamethoxam, Thiodicarb, Thiofanox sulfone, Thiofanoxsulfoxide, Thiometon sulfone, Thiometon sulfoxide, DMST (degr. tolylfluanid),Triadimefon, Triazophos, Tricyclazole, Trifloxystrobin, Triflumizole, Tritoconazole,Vamidothion sulfone, Zoxamide.
Modified method UPLC-MS-MSbased on
I. STEVEN J.LEHOTAY,KATERJNA MASTOVSKA ANDALAN R. LIGHTFIELD:JOURNAL OF AOACINTERNATIONAL VOL. 88, NO.2,2005.
2) C.LENTZA-RIZOS: JOURNALOF AOAC INTERNATIONAL77(5), 1096, (1994).
3. SANCO/12495/2011 of theEuropean Commission
S.Olives !. Determination of 150 pesticide residues
Acephate, Acetamiprid Aidicarb sulfone, Aldicarb sulfoxide, Azamethiphos,Azinphos methyl, Azoxystrobin, Azaconazole, Bitertanol, Boscalid,Bromuconazole, Buprimate, Buprofezin, Butocarboxim sulfone, Butocarboximsulfoxide, Carbaryl, Carbendazim, Carbofuran, Carbofiiran Shydroxy,Carpropamid, Chlorbomuron, Chlorfenvinphos cis, Chlorfenvinphos trans,Chlorpyrifos, Chlorpyrifos-methyl, Clotdianidin, Cyazofamid, Cymoxanil,Cyproconazole, Demeton-O-sulfoxide, Demeton-S- methyl sulfone, Desmedipham,Diazinon, Dichlofluanid, DMSA (degr. dichlofluanid), Dichlorvos, Diethofencarb,Difenoconazoie, Diflubenzuron, Dirnethoate, Dimethomorph, Dimoxystrobin,Diniconazoie, Epoxiconazole, Ethiofencarb sulfone, Ethiofencarb sulfoxide,Famoxadone, Fenamidone, Fenamiphos, Fenarimol, Fenbuconazole, Fenoxycarb,Fenpyroximate, Fenthion, Fenthion sulfoxide, Fenthion Oxon, Fenthion Sulfon,Fludioxonii, Flufenacet, Flufenoxuron, Fluquinconazole, Flusilazole, Flutolanil,Flutriafol, Fosthiazate, Furathiocarb, Halofenozide, Hexaconazole, Hexaflumuron,Hexythiazox, Imazalil, Imidacloprid, Iprovalicarb, Isoprothiolane, Isoxaflutole,Isoxathion, Kresoxim-methyl, Linuron, Malathion, Mefenacet, Mepanipyrim,Mephosfolan, Mepronil, Metalaxyi, Metconazole, Meihamidophos, Methiocanb,Methiocarb sulfone, Methiocarb sulfoxide, Methomyl, Methoxyfenozide,Metoxuron, Monocrotophos, Monolinuron, MyclobutaniL Nitenpyram, Nuarimol,Omethoate, Oxadixyl, Oxarnyl, Oxycarboxin, Oxydemeton-methyl, Paclobutrazole,Penconazole, Pencycuron, Phenmedipham, Phorate sulfoxide, Phosphamidon,Pirimicarb, Pirimicarb desmethyl, Pirimiphos-methyl, Prochloraz, Profenofos,Propargite, Propiconazole, Propoxur. Propyzamide, Pyraclostrobin, Pyrazophos,Pyridaphenthion, Pyrifenox, Pyruneihanil, Pyriproxyfen, Spinosad A, Spinosad D,Spirodiclofen, Tebuconazole, Tebufenpyrad, Tebufenozide,, Teflubenzuron,Tetraconazole, Thiabendazole, Thiacloprid, Thiarnethoxam, Thiodicarb, Thiofanoxsulfone, Thiofanox sulfoxide, Tliiometon sulfone, Thiometon sulfoxide,Tolylfluanid, DMST {degr. tolylfluanid), Triazophos, Tricyclazole, Triflumizole,Triflumuron, Trichlorphon. Tritoconazole, Vamidothion sujfone, Zoxamide
Modified method UPLC-MS-MSbased on
I.STEVEN J.LEHOTAY,KATERINA MASTOVSKA ANDALANR. LIGHTFIELD:JOURNAL OF AOACINTERNATIONAL VOL. 88, NO.2,2005.
2. C.LENTZA-RIZOS: JOURNALOF AOAC INTERNATIONAL77(5), 1096, (1994).
3. SANCO/12495/2011 of theEuropean Commission
2. Determination of 13 organochloride pesticide residues
[a BHC, b BHC, y BHC, d BHC, heptachlor epoxide, 4,4' DDE, 4,4' ODD,4,4' DDT, entrin, dieldrin, endosulfan a, endosulfan b, endosulfan sulphate]
Modified method based on
A.StLeHotay Journal of AOACInternational 88(2).615 mod.
B. Lentza-Rizos Journal of AOACInternational 77(5).I096(1994)mod.
C. DFG Manual of PesticideResidues Analysis METHOD S-9.
Page 3 to 8 Annex F1/B15 to the Certificate ESYD No 44-4 21.10.2013
Materials/ProductsTested
Types of Test / Properties Applied Standards /Techniques
6.Tobacco 1. Determination of 126 pesticide residues
Acetamiprid, Aldicarb sulfoxide, Azinphos methyl, Azoxystrobin, Azaconazole,Boscalid, Bramuconazole, Buprimate, Buprofezin, Butocarboxim sulfoxide, Carbaryl,Caibendazim, Carbofuran, Carboftiran 3-hydroxy, Capropatnid, Chlorfenvinphos cis,Chlorfenvinphos trans, Chtorpyrifos-niethyl, Clothianidin, Cymoxanil, Cyproconazole,Cyprodinil, Demeton-O-sulfoxide, Desmedipham, Diazinon, Dicrotophos, Diethofencarb,Difenoconazoie, Diflubenzuron, Dimethoate, Dimethomorph Dimoxystrobin,Diniconazole, Diuron, Dodemorph, Epoxiconazole, Ethiofencarb sulfone, Ethiofencarbsulfoxide, Ethion, Ethiprole, Ethirimol, Famoxadone, Fenamidone, Fenazaquin,Fenbuconazole, Fenoxycarb, Fenpropidin, Fenpropimorph, Fenpyroximate, Fenthion,Fenthton sulfoxide, Fludioxonil, Flufenacet, Flufenoxuron, Fluquinconazoie, Flusilazole,Flutolanil, Flutriafol, Fosalon, Fosthiazate, Furathiocarb, Halofenozide, Hexaoonazole,Hexythiazox, Imazalil, Indoxacarb, Isoprothiolane, Isoxathion, Kresoxim-methyl,Linuron, Malathion, Mefenacet, Mepanipyrim, Mephosfolan, Mepronil, Metalaxyl,Metconazole, Methidathion, Methiocarb, Methoxyfenozide, Metobromuron, Metoxuron,Monoiinuron, Nuarimol, Oxadixyl, Oxycarboxin, Penconazole, Pencycuron,Phenmedipham, Phorate sulfoxide, Phospharnidon, Picoxystrobin, Piperonyl buloxide,Pirimicarb, Pirimicarb desmethyl, Pirimiphos-rnethyl, Prochloraz, Profenofos, Propargite,Propoxur, Propyzamide, Pyraclostrobin, Pyrazophos, Pyridaben, Pyridaphenthion,Pyrifenox, Pyrimethanil, Pyriproxyfen, Spinosad A, Spinosad D, Tebuconazole,Tebufenpyrad, Tebufenozide, Thiabendazole, Thiacloprid, Thiodicarb, Thiofanox sulfone,Thiofanox sulfoxide, Thiomaon sulfone, Thiometon sulfoxide, Triazophos, Tricyclazole,Trinoxystrobin, Triflumizole, Tritoconazole, Quinoxyfen, Zoxamide
Modified method UPLC-MS-MSbased on
1. Jeong-Ming Lee et a!.: Journal ofChromatography A, 1187 (2008) 25-33,
2. SANCO/12495/2011 of theEuropean Commission
7. Cereals, flour,legumes, dry nuts
1. Determination of 165 pesticide residuesAcephate, Acetamiprid, Aldicarb suifone, Azaconazole, Azamethiphos, Azinphos-methyl, Azinphos-ethyl, Azoxystrobin, Boscalid, Bromuconazole, Bupirimate,Buprofezin, Butocarboxim sulfoxide, Caibendazim, Carbofuran, Carbofuran 3hydroxy,Carpropamid, Chlorbromuron, Chlorfenvinphos, Chlorpyrifos-methyl, Gofentenzine,Clothianidin, Cyazofamid, Cyrnoxanil, Cyproconazole, Cyprodinil, Demeton-S- methylsulfone, Desmedipham, Diazinon, DMSA {degr. dichlofiuanid), Dichlorvos, Dicrotophos,Diethofencarb, Difenoconazoie, Diflubenzuron, Dimethoate, Dimethomorph,Dimoxystrobin, Diniconazole, Diuron, Dodemorph, Epoxiconazole, Ethiofencarb sulfone,Ethiofencarb sulfoxide, Ethion, Ethiprole, Ethirimol, Etofenprox, Famoxadone,Fenamidone, Fenamiphos, Fenazaquin, Fenbuconazole, Fenhexamid, Fenoxycarb,Fenpropidin, Fenpropimorph, Fenpyroximate, Fenthion sulfoxide, Fludioxonil,Flufenacet, Flufenoxuron, Fluquinconazole, Flusilazole, Flutolanil, Flutriafol,Forchlofenuron, Fosthiazate, Furathiocarb, Halofenozide, Hexaconazole, Hexythiazox,Imazalil, Imidacloprid, Indoxacarfa, Iprovalicarb, Isoprothiolane, Isoxaflutole, Isoxathion,Kresoxim-rnethyl, Linuron, Lufenuron, Malathion, Mefenacet, Mepanipyrim,Mephosfolan, Mepronil, Metalaxyl, Metconazole, Methidathion, Methiocarb, Methiocarbsulfone, Methomyl, Methoxyfenozide, Metobromuron, Metoxuron, Monocrotophos,Monoiinuron, Myclobutanil, Nuarimol, Omethoate, Oxadixyl, Oxamyl, Oxamyl-oxime,Oxycarboxin, Oxydemeton-methyl, Paclobutrazole, Penconazole, Pencycuron,Phenmedipham, Phorate sulfoxide, Phosalone, Phosmet, Phosphamidon, Picoxystrobin,Piperonyl butoxide, Pirimicarb, Pirimicarb desmethyl, Pirimiphos-methyl, Prochloraz,Profenofos, Propargite, Propamocarb, Propiconazole, Propoxur, Propyzamide,Pymetrozine, Pyraclostrobin, Pyrazophos, Pyridaben, Pyridaphenthion, Pyrifenox,Pyrimethanil, Pyriproxyfen, Quinoxyfen ,Spinosad A, Spinosad D, Spirodiclofen,Spiroxamine, Spiromesifen, Tebuconazole, Tebufenozide, Tebufenpyrad, Telraconazole,Thiabendazole, Thiacloprid, Thiamethoxam, Thiophanate-methyl, Thiofanox sulfone,Thiofanox sulfoxide, Thiometon sulfone, Thiometon sulfoxide, DMST (degr.tolylfluanid), Triadimefon, Triadimenol, Triazophos, Trichlorphon, Tricyclazole,Trifloxystrobin, Trifiumizole, Triforine. Triticonazole, Vamidothion sulfone,Vamidothion sulfoxide, Zoxamide
In house method UPLC-MS-MSbased on
1. Steven J. Lehotay, KaterinaMastovska & Alan R. Lightfield:Journal of AOACINTERNATIONAL Vol. 88, No. 2,2005.
2. SANCO/12495/2011 of theEuropean Commission
8. Foodstuffs (bakeryproducts, fried potato,dry nuts)
Determination of AcrylamideIn house method UPLC-MS-M1.Steven J. Lehotay, KaterinaMastovska & Alan R.Lightfield: Journal of AOACINTERNATIONAL Vol. 88No2,2005
2., Katerina Mastovska &Steven J. Lehotay, Journal ofAgriculture and FoodChemistry, 2006, 54, 7001-7008.
Page 4 to 8 Annex F1/BI5 to the Certificate ESYD No 44-4 21.10.2013
Materials / ProductsTested
1. Foods and animal feed
2. Milk and dairyproducts
3.Milk and infant & babyfoods containing milk
4. Meat and MeatProducts
Types of Test / Properties
Microbiological Testing
1. Enumeration of total viable count
2. Coliforms (Enumeration)
3. E. coli (Enumeration)
4. Bacillus cereris (Enumeration)
5. Staphylococci coagulase positive Staphylococcus aureus and otherspecies (Enumeration))
6. Yeasts / Molds (Enumeration)
7. Yeasts / Molds (Enumeration)
8. Salmonella spp (detection)
9. Lisleria spp (detection)
10. L monocytogenes (detection)
1 1 . E.coli O157-.H7 (detection)
1 2. Slaphyhcoccal enterotoxin (detection)
1 3. Entero bacteriaceae (Enumeration)
14. C.perjringens (enumeration)
1 5. Anaerobic sulfite reducing bacteria and clostridia (enumeration)
1 6. Campylobacter spp (detection)
1 7. Mesophilic tactic acid bacteria (enumeration)
1 8. Lmonotytogenes (enumeration)
19. Psychrotrophic microorganisms (enumeration)
20. Detection of Salmonella spp.
2 1 . Detection ofListeria monocytogenes1 . Yeasts / Molds (Enumeration)
1. Cronobacter sakazakii (detection)
1 . Pseudomonas spp (Enumeration)
2. Brochothm thermosphacia ^enumeration)
Applied Standards /Teduiiques
ISO 4833: 2003
ISO 4832:2006
ISO 16649-2: 2001
1507932:2004
ISO 6888-2: 1999 (37°C) (+amd2003)
ISO 21 527-1 (a,v>0,95)& 21527-2 :2008(aw<0,95)AFNOR NF V08-036:2003(aw<0,96)AFNOR No BIO 12/23-05/07AFNOR No BIO 12/16-09/05AFNOR No BIO 12/2-06/94
AFNOR no BIO 12/9 -07/02AFNOR No BIO 12/2-06/94
AFNOR no BIO 12/8-07/00
AOAC no 070404
ISO 2 1528-2:2004
ISO 7937:2004
ISO 15213:2003 (37°Q
ISO 10272-1:2006
ISO 15214:1998
ISO 11290-2:1998 + Amd.1:2004
(37°C)
ISO 17410:2001
ISO 6579:2002
ISO11290-l:1996/Amdl:2004ISO 661 1:2004
ISO 22964:2006
ISO 19250:2010
ISO 13722:1996
Page 5 to 8 Annex FI /B15 to the Certificate ESYD No 44-4 21.10.2013
Materials / ProductsTested
5. Foods, animal feed andenvironmentalproduction samples(except primaryproduction stageenvironment)
6. Animal faeces andenvironmental samplesfrom the primaryproduction stage
7. Salmonella isolates
8. Water (water forhuman consumption,surface water, poolwater)
9. Sea water
10. Treated waste waterfrom treatment plant
11. Water forhemodialysis andrelevant treatments
Types of Test / Properties
1. Detection of Salmonella spp.
1 .Detection of Salmonella spp.
1. SerotypingofS. Enteritidis, S. Typhimurium
\ Sulfite reducing clostridia (spores)
2. Salmonella spp (presence/absence )3. Legionella spp (detection - enumeration)
4. P.aemginosa (enumeration)
5. Enumeration of culiurable microorganisms at 37 and 22 °C
6. Coliforms (Enumeration)
7. Faecal Coliforms (Enumeration)
8. E.Coli (KaTct|i£Tpr]an.)
9. Enumeration of Escherichia coli and coliform bacteria
10. Intestinal enterococci (Enumeration)
1 1 . Detection and enumeration of Cl. perfringens (including spores)
1 . Enumeration of culturable microorganisms at 37 and 22 °C
2. Coliforms (Enumeration)
3. Faecal Coliforms (Enumeration)
4. E.Coli (Kara|ieTpr|cm,)
5. Intestinal enterococci (Enumeration)
1. Detection and enumeration of intestinal enterococci
Enumeration of total cultutabie microorganisms at 20 °C (± 2°C)
Applied Standard^/Techniques
AFNORBKR 23/07-10/11
ISO 6579:2002/Amd 1 :2007,ANNEX D
White Kauffhiann Le MinorScheme 2007, 9n EKooon
ISO 6461-2:1986ISO 19250:2010
15011731:1998
ISO 11731-2:2004
ENASO 16266:2006
ISO 6222: 1999
ISO 9308-1:2000
APHA 9222D: 2005
ISO 9308-1 :2000/cor 2007
AFNORBRD 07/20-03/11
ISO 7899-2: 2000
Annex III of J.M.D. Y2/2600/2001,as modified, completed and validuntil nowISO 6222: 1999
ISO 9308-1:2000
APHA 9222D: 2005
ISO 9308-1 :2000/cor 2007
ISO 7899-2: 2000
ISO 7899-2:2000
ISO 13959:2009
Page 6 to 8 Annex F1/BI5 to the Certificate ESYDNo 44-4 21.10.2013
Materials / ProductsTested
Types of Test/ Properties Applied Standards /Techniques
Sampling
1. Samples from surfacesusing swabs and contactplates
1. Horizontal methods for sampling techniques for microbiologytests
ISO 18593:2004
Biological tests
1. Cotton (seeds) Detection of GMO (detection of CMV 35S promoter,terminator, PAT gene, BAR gene, and element CTP2-CP4EPSPS)
NOSInternal method OB.04.611 basedon:1.ISO 21569:20052. Gaudron et al., Eur. Food ResTechnol, 229: 295-305,20093. Grohmann et at., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al., Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Kodama et. al., Journal of AOACInternational vol. 92, No. 1,20096. Macherey-Nagel, NucleoSpinFood kitusing Real-Time PCR
2. Potato and potatoproducts (food, feed, rawmaterials)
Detection of amylopectin potato event EH 92-527-1Internal method OB.04.6112based on1. 15021569:20052. Event-specific Method for theQuantification of AmylopectinPotato Event EH92-527-I UsingReal-time PCR, Protocol EH92-527-1-Community ReferenceLaboratory for GM Food and Feed,CRLVL09/05VP3. Macherey-Nagel, NucleoSpinFood kit.using Real-Time PCR
3. Rice and rice products(food, feed, raw materials)
Detection of Rice
Line LLRICE 62
GM-eventLL601
Bt 63 Rice
Internal method OB.04.6113based on1.15021569:20052. Event-specific method for thequantification of rice line LLRICE62 using Real Time PCR (EventProtocol LLRICE62 - Communityreference laboratory for GM Foodand Feed)3. Multiplex real time PCR for thesimultaneous detection andquantification of DNA from threetransgenic rice species andconstruction and application of anartificial oligonucleotide as referencemolecule. R. Koppel, F. Zimmerli &A. Breitenmoser, Eur. Food ResTechnol (2010) 230:731-7364. Report on the validation of anevent-specific method for thedetection method foridentification of Rice GM-eventLLRICE 601 using a Real TimePCR assay. CommunityReference Laboratory for GMFood and Feed
Page 7 to 8 Annex F I / B I S to the Certificate ESYD No 44-4 21.10.2013
Materials/ProductsTested
Types of Test / Properties Applied Standards /Techniques
Rice and rice products(food, feed, raw materials)
(continue)
5. Report on the verification ofthe performance of a method forthe detection of Bt 63 Rice usinga real time PCR assay.Community Reference Laboratoryfor GM Food and Feed. CRL-EM-02/06, verification report RiceBt636. Macherey-Nagel, NucleoSpinFood kitusing Real Time PCR
4. Soya and soya products(seeds, food, feed, rawmaterials)
Detection of GMO (CMV 35$ promoter, NOS terminator, PAT gene,BAR gene, CTP2-CP4EPSPS element and S-adenosy!-L-methioninesynthesase promoter)
Internal method OB.04.611 basedon:1.15021569:20052. Gaudron et al., Eur. Food ResTechnol, 229: 295-305,20093. Grohmann et al., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al., Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Macherey-Nagel, NucleoSpinFood kit6. C. Bahrdt, et al., Anal BioanalChem 396:2103-2112,20107. Kodama et. al., Journal of AOACInternational vol. 92, No. 1, 2009
using Real-Time PCR
5. Corn and com products(seeds, food, feed, rawmaterials)
Detection of GMO (CMV 35S promoter, NOS terminator, PAT gene,BAR gene and CTP2-CP4EPSPS element)
Internal method OB.04.611 basedon:1.15021569:20052. Gaudron et al., Eur, Food ResTechnol, 229: 295-305,20093. Grohmann et al., J. Agric FoodChem, 57: 8913-8920,20094. Sebah et al.. Project GMOseek,Development of screening methodsfor GMOs, Final Report, 20105. Kodama et. al., Journal of AOACInternational vol. 92, No. 1,20096. Macherey-Nagel, NucleoSpinFood kit
using Real-Time PCR
Site of assesement : Laboratory permanent premises, Industrial area, Markopoulo, AttikiApproved signatories: A. Giannousios, C. Alexandrou, KPapanotas, D.Koraki
This Scope of Accreditation replaces the previous one dated 1 .3.20 1 3The Accreditation Certificate No. 44-3 to EL£TOgp/lEC 1 7025:200^, is valid until 26.1 1 .2017.
Athens,, 21. 10.2013
atories Accreditation Division
Page 8 to 8 Annex F1/B15 to the Certificate ESYD No 44-4 21.10.2013