Hemoglobin Experiment

In the hemoglobin experiment, blood was mixed with potassium ferricyanide in a test tube. This

tube is then placed in a spectrophotometer (seen above) and the amount of cyanmethemoglobin is

determined by measuring the amount of transmittance (%T) of light that passes through the

test tube. The %T can be used to determine the number of grams of hemoglobin that is in 100 ml of

that blood. (See problem on slide 3.)

PROBLEM

(answers are on following slide)

1. If the %T is 44, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on

slide 2.)

2. How many ml of O2 can this same blood carry?

3. If the %T is 30, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2, look at the calculation at the bottom.)

4. How many ml of O2 can this same blood carry?

PROBLEM

1. If the %T is 44, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on

slide 2.)

14.3 g

2. How many ml of O2 can this same blood carry?

14.3 g (Hgb) X 1.34 = 19.16 ml O2

3. If the %T is 30, how many grams of hemoglobin can 100 ml of this blood carry? (See chart on slide 2, look at the calculation at the bottom

(100%-30%) x .256 = 17.92 g

4. How many ml of O2 can this same blood carry?

17.92 x 1.34 = 24.01 ml O2